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Citations for Takara Bio :
4601 - 4650 of 5458 citations for Rat Insulin Like Growth Factor Binding Protein 4 IGFBP4 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... 500 ng of total RNA was used for the cDNA synthesis reaction using PrimeScript™ RT Master Mix kit (TaKaRa RR036A) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Pooled samples were processed using SMARTer chemistry (SMART Seq® v4 Ultra® Low Input RNA Kit for Sequencing, Takara Clontech), according to manufacturer’s instructions to generate cDNA libraries for mRNA sequencing ...
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bioRxiv - Cell Biology 2021Quote: ... lentiviral shRNA expression constructs were transfected together with packaging vectors (VSV-G, RSV-Rev and GAG-Pol) into 293T producer cells using CalPhos Transfection Kits (Takara Bio) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... 1 μg of total RNA from each condition was converted into cDNA using PrimeScript RT reagent with gDNA Eraser kit (Takara Clontech) and MJ Research PTC-220 Dyad PCR System (Conquer Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... with primers BA1569/BA1570 and cloned into RSFDuet-1 using BamHI/EcoRI sites with an In-Fusion HD Cloning Plus kit (Takara Bio). To make pBA2276 (His6-Designed zinc finger) ...
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bioRxiv - Neuroscience 2022Quote: AAVs were packaged via calcium phosphate transfection of HEK293 cells as detailed in (McClure et al 2011) and purified using an AAVpro purification kit (Takara Bio).
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bioRxiv - Developmental Biology 2022Quote: ... An aliquot of 1 μg of the total RNA was used to synthesize cDNA using PrimeScript™ RT reagent Kit with gDNA eraser (Takara). qRT-PCR analysis was performed on a StepOnePlus Real-Time PCR system (Applied Biosystems ...
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bioRxiv - Cell Biology 2022Quote: Cells cultured in either 12- or 24-well plates were washed twice with cold PBS and harvested using a TaKaRa MiniBEST universal RNA extraction kit (Takara, Japan). RNA was purified using the same kit according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... was used as the template for real-time RT-PCR performed according to the manufacturer’s protocol using the One Step TB Green PrimeScript PLUS RT-PCR kit (Takara, Cat# RR096A) and the following primers ...
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bioRxiv - Pathology 2022Quote: ... 5 ng of total RNA was used with the SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian (TAKARA, Japan), according to the manufacture’s instruction ...
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bioRxiv - Biochemistry 2022Quote: ... The barcodes were introduced immediately after the Tae1 coding sequence via Gibson assembly cloning (In-Fusion HD Cloning Kit – Takara Bio). Our Next-Generation sequencing strategy comprised two stages ...
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bioRxiv - Biochemistry 2022Quote: ... Normalization was made by measuring the β-Gal activity after 1-hour incubation of 7.5 µl of cellular extract with 50 µl of β-Gal substrate (β-Gal detection kit II, Clontech).
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bioRxiv - Immunology 2022Quote: ... The g block and linearized plasmid backbone were ligated together using the In-fusion HD cloning kit (Takara Bio, catalog #638920).
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bioRxiv - Microbiology 2022Quote: ... The PCR product was purified and ligated into the pET15b vector with a C-terminal His tag to create plasmid pET-AdpA by using the ClonExpress™ II One Step Cloning Kit (TaKaRa). The plasmid was transformed into E ...
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bioRxiv - Immunology 2022Quote: ... Total RNA was then subjected to genomic DNA (gDNA) elimination and reverse-transcription according to PrimeScript™ RT reagent kit protocols (Takara). Briefly ...
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bioRxiv - Plant Biology 2022Quote: ... . cDNA was synthesized by the use of PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) according to the reverse transcription protocol (Takara). The resulting cDNA was subjected to relative quantitative PCR using a SYBR Premix Ex TaqTM kit (TaKaRa ...
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bioRxiv - Plant Biology 2022Quote: ... The 1 μg total RNAs were used to synthesize cDNA with the PrimeScript™RT reagent Kit with gDNA Eraser (Takara). qRT-PCR was carried out with SYBR Premix Ex Taq II Premix (Takara ...
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bioRxiv - Molecular Biology 2022Quote: ... ceranae-inoculated workers’ midguts at 7 dpi and 10 dpi were respectively isolated using RNA Extraction Kit (TaKaRa company, Dalian, China). cDNA was synthesized through reverse transcription with oligo dT primer and used as templates for qPCR assay ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA was synthesized by the use of PrimeScript™RT reagent Kit with gDNA Eraser (Perfect Real Time) according to the reverse transcription protocol (Takara). The resulting cDNA was subjected to relative quantitative PCR using a SYBR Premix Ex Taq™ kit (TaKaRa ...
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bioRxiv - Immunology 2022Quote: ... added with RT-PCR master mix and IgG VH/VL primers per well and performed with RT-PCR following the one step RT-PCR kit protocol (Takara, RR057A). Then ...
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bioRxiv - Biochemistry 2022Quote: ... encoding a KkRseP derivative having an internal PA14 tag: Lys54-EGGVAMPGAEDDVV-His55) was constructed by using the In-Fusion HD Cloning Kit (Takara Bio) as follows ...
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bioRxiv - Bioengineering 2022Quote: ... Purified insert DNA was cloned into the linearized modRNA plasmid (5MCS3)(K et al., 2018) using the In-Fusion Cloning Kit (Takara Bio). The DNA template for modRNA synthesis was PCR amplified from the successfully cloned 5MCS3 plasmid followed by PCR purification using DNA Clean & Concentrator-5 (Zymo Research) ...
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bioRxiv - Cancer Biology 2022Quote: ... Truncations of ENDOD1 and mutations of TP53 were obtained by fusing different PCR fragments using In-Fusion cloning kit (Clontech, 639650). Primers used in this study were listed in Supplementary Table S3.
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bioRxiv - Neuroscience 2021Quote: Isolated RNA was prepared for RNA sequencing using the SMART-seq v4 Ultra Low Input RNA kit (Takara Bio USA 634888). The Nextera XT DNA Library Prep Kit (Illumina FC-131-1024 ...
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Targeted attenuation of elevated histone marks at SNCA alleviates α-synuclein in Parkinson’s diseasebioRxiv - Neuroscience 2020Quote: ... the collected nuclei were pelleted down immediately and lysed in 100 μL RA1 buffer from the Nucleo Spin RNA XS Kit (Takara, 740902.50). This kit is optimized for isolating RNA from as little as one cell ...
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bioRxiv - Immunology 2020Quote: ... and RNA-Seq libraries were prepared from 250 pg of total RNA using SMARTer Stranded Total RNA-Seq Kit v2 (Takara Bio). After QC using TapeStation 4200 High Sensitivity DNA tapes (Agilent ...
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bioRxiv - Microbiology 2021Quote: ... 1 μg of RNA was used for library preparation with the SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian (Takara Bio), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and a library for whole-genome sequencing was prepared using the PrepX ILM DNA library kit (Takara Biosciences, Mountain View, CA) for the Apollo 324 next-generation sequencing (NGS ...
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bioRxiv - Molecular Biology 2019Quote: ... total RNA (1 μg) was reverse-transcribed into cDNA using the PrimeScript RT reagent Kit with gDNA Eraser (Takara, Shiga, Japan), and relative mRNA expression was determined by real-time PCR using FastStart Universal SYBR Green Master [Rox] (Roche ...
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bioRxiv - Molecular Biology 2020Quote: ... and hERG mutants were generated by PCR-mediated site-directed mutagenesis using an In-Fusion HD Cloning Kit (Takara, Otsu, Japan) as described previously (Kume et al. ...
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bioRxiv - Molecular Biology 2020Quote: The ORFs encoding PxRdls were cloned into a pT7TS vector using the In-Fusion HD Cloning Kits (Takara Biotechnology, Dalian, China) and ...
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bioRxiv - Cell Biology 2020Quote: ... an MBP-tag and a TEV protease recognition site (His-MBP-TEV) by Infusion® HD Cloning kit (Takara Bio, USA). The fidelity of the constructs was confirmed by gel electrophoresis and sequencing.
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bioRxiv - Microbiology 2021Quote: ... Then PCR application using specific primer pairs (Supplemental Table 2) designed for H7N9 virus and Takara One-step RT-PCR kit (Takara, China) segment by segment ...
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bioRxiv - Molecular Biology 2020Quote: ... Amplicons were isolated from the agarose gel using the PCR cleanup and gel extraction kit (ClonTech, Takara USA, Mountain View, CA) and the nucleotide sequence of amplicons determined by Sanger direct sequencing (GENEWIZ ...
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bioRxiv - Molecular Biology 2020Quote: ... Amplicons were isolated from the agarose gel using the PCR cleanup and gel extraction kit (ClonTech, Takara USA, Mountain View, CA) and the nucleotide sequence of amplicons determined by Sanger direct sequencing (GENEWIZ ...
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bioRxiv - Microbiology 2020Quote: ... PCR amplification on the cleaned libraries was performed as follows: A reaction mix using LA Takara Hot Start kit (Takara Bio) was prepared according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... zroraa LBD deletion DN -: 5′- ctgattatgatctagagtccaggccggattgatcagg-3 and inserted into a Tol2-lyzC-mcherry-2A backbone by using infusion cloning kit (Takara #638920). The construction method for neutrophil-specific Cas9 expression and the guide RNA expression fish lines has been described in our previous study [40] ...
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bioRxiv - Immunology 2020Quote: ... Tcrb amplicons were prepared using a 5’RACE-based protocol with the SMARTer Mouse TCR α/β Profiling Kit (Takara #634402) following the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Cells cultured in either 12 or 24-well plates were washed twice with cold PBS and harvested using TaKaRa MiniBEST Universal RNA extraction kit (Takara, Japan). RNA was purified using the same kit according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: 800pg total RNA from each sample pool was subjected to library construction according to manufacturer’s instructions (SMARTer® Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian, Takara Bio). Libraries were barcoded ...
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bioRxiv - Microbiology 2021Quote: ... was used as the template for real-time RT-PCR performed according to the manufacturer’s protocol using the One Step TB Green PrimeScript PLUS RT-PCR kit (Takara, cat# RR096A) and the following primers ...
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bioRxiv - Microbiology 2021Quote: ... The copy number of viral RNA was standardized with a SARS-CoV-2 direct detection RT-qPCR kit (Takara, cat# RC300A). The fluorescent signal was acquired using a QuantStudio 3 Real-Time PCR system (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2021Quote: ... and the reverse transcription products were amplified using the Agilent-Strata gene MxReal-Time qPCR system with a PrimeScript™ RT Reagent Kit (Takara). Reverse transcription products were amplified using an Agilent-Strata gene MxReal-Time qPCR system with TB Green® Premix Ex Taq™ II (Tli RNaseH Plus ...
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bioRxiv - Microbiology 2021Quote: ... The amplified fragments were cloned into the SmaI site of pPTR I using an In-Fusion HD cloning kit (Takara Bio). Transformants were selected on minimal agar medium with pyrithiamine.
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bioRxiv - Microbiology 2021Quote: ... The amplified fragment was cloned into the SmaI site of pPTR I using an In-Fusion HD cloning kit (Takara Bio). The pPTR I-GFP-nrdA plasmid was used to transform the A ...
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bioRxiv - Microbiology 2021Quote: ... The amplified cDNAs were cloned into the indicated plasmids using an In-Fusion HD cloning kit (Clontech, Mountain View, CA, USA). The sequences of all plasmids were confirmed by Eurofins Genomics (Tokyo ...
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bioRxiv - Bioengineering 2020Quote: ... 50 ng of total RNA from each sample was amplified and synthesised to cDNA with SMART-seq (SMART-Seq v4 Ultra Low Input RNA Kit, Takara Bio). Then ...
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bioRxiv - Bioengineering 2020Quote: ... Viral vectors were purified using an AAVpro® Purification Kit (All Serotypes) (catalog no. 6666; Clontech Laboratories, Mountain View, CA, USA) 48 hours after viral transduction ...
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bioRxiv - Molecular Biology 2022Quote: ... Equal amounts of RNA samples (200 ng) were used to generate complementary DNA using a PrimeScript RT reagent kit (TaKaRa Bio) with oligo-dT and random primers ...
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bioRxiv - Microbiology 2022Quote: ... NIH) and then cloned into pLVX-IRES-zsGreen1 vector using an In-Fusion HD cloning kit (Clontech, Mountain View, CA, USA). The GAD mutant of ORF1 inactivating the polymerase was generated by QuikChange (Stratagene ...