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Citations for Takara Bio :
4451 - 4500 of 5592 citations for Mouse Antigen peptide transporter 2 TAP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Total RNA (0.5ng/mL) was added to lysis buffer from the SMART-seq V4 Ultra Low Input RNA kit for Sequencing (Takara), and reverse transcription was performed followed by PCR amplification to generate full-length amplified cDNA ...
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bioRxiv - Immunology 2020Quote: ... RNA treated with DNase and the cDNAs were synthesized with PrimeScript™ RT reagent Kit with gDNA Eraser (TaKaRa, #RP047A). Samples without reverse transcriptase were also added as control ...
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bioRxiv - Cell Biology 2021Quote: Transfection of Halo-tagged AnkB440 plasmids were conducted in HEK293T cells grown in 10 cm culture plates using the calcium phosphate transfection kit (Takara) and 8 µg of plasmid ...
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bioRxiv - Developmental Biology 2021Quote: ... and cloned into into modified pJet:attB:mCherry vector (Roberts et al., 2014) containing phiC31 attB site and mCherry reporter using In-Fusion HD Cloning Kit (TaKaRa/Clontech) according to the manufacture instructions ...
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bioRxiv - Genetics 2019Quote: ... luteus isolates was used in a genome walking procedure using the Universal GenomeWalker™ kit (Clontech, Mountain View, California, US) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... Libraries from 4-cell embryos were also prepared using 18-30 nt gel purified RNA using the SMARTer smRNA-Seq Kit (Clontech) and NEXTflex-Small-RNA-Seq (New England Biolabs ...
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bioRxiv - Genomics 2019Quote: ... The 48-μL preparation volume for reverse transcription (RT) contains 1X SMARTer Kit 5X First-Strand Buffer (5X; Clontech, 634936), 2.5-mM SMARTer Kit Dithiothreitol (100 mM ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The concentrations of the host and the parasitic RNAs were measured by RT-qPCR (PrimeScript One Step RT-PCR Kit (TaKaRa)) with sequence-specific primers (Supplementary text).
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bioRxiv - Genetics 2020Quote: ... The products obtained were subsequently subjected to semi-nested PCR with a LA Taq Hot Start polymerase kit (TaKaRa Biotechnology). The first round of amplification was performed with primers ...
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bioRxiv - Immunology 2021Quote: ... 293T Lenti-X cells were seeded onto 10-cm Petri dishes and transiently transfected (CalPhos™ Mammalian Transfection Kit, Clontech Laboratories ...
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bioRxiv - Neuroscience 2020Quote: ... And then the DNA fragment encoding 4R1N tau were inserted into the linearized pHR-mCh-Cry2Olig backbone using In-Fusion Cloning Kit (Takara). The resulting constructs were fully sequenced to confirm the absence of unwanted substitutions.
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bioRxiv - Immunology 2022Quote: ... or the expression vector LentiCRISPRv2 (coding for guideRNA) in a 3:1:3 ratio for 8 hours using calcium phosphate transfection (CalPhos Mammalian Transfection Kit, Takara Bio Europe ...
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bioRxiv - Developmental Biology 2022Quote: ... first-strand cDNA synthesis and subsequent cDNA amplification by 22 cycles of polymerase chain reaction (PCR) were performed using the SMARTer stranded RNA-seq kit (Takara). The synthesized first-strand cDNA and amplified cDNA were purified using Ampure XP Beads (Beckman Coulter) ...
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bioRxiv - Immunology 2022Quote: ... RNA concentrations were determined using the Qubit high-sensitivity RNA assay and small RNA libraries were created using the Clontech SMARTer smRNA-Seq preparation kit according to manufacturer instructions (Takara). Small RNA inserts were further enriched using AMPure bead selection (Beckman Coulter ...
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bioRxiv - Immunology 2022Quote: ... and RNA quality (Bioanalyzer) were measured again before proceeding with cDNA libraries construction using the SMARTseq v4 Ultra-Low Input kit (Clontech).
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bioRxiv - Molecular Biology 2022Quote: ... Aliquots of 2.0 ug of the total RNA were collected for cDNA synthesis by using Clontech Oligo dT cDNA synthesis kit (Takara Bio USA ...
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bioRxiv - Biochemistry 2022Quote: ... and the next 1μg of total eligible RNA was reverse transcribed using the cDNA synthesis kit (Code No. 6110A, Takara, Japan) for quantitative real-time PCR (RT-qPCR) ...
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bioRxiv - Cell Biology 2022Quote: ... The amplicons were then cloned into the pJFRC7 5x UAS vector (ref) using Infusion cloning kit (Takara Bio, catalog #638948). Sequenced clones were sent to BestGene for injection and stock generation.
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bioRxiv - Biochemistry 2022Quote: ... 127926 and 127924). shTnsB mutants (Fig. 7) were generated in the pHelper vector using the In-Fusion cloning kit from Takara and primers containing the desired point mutations ...
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bioRxiv - Biochemistry 2022Quote: A DNA fragment coding HA-tagged Dcp2 was amplified by PCR and cloned into pIExZ vector (Izumi et al, 2020) by In-fusion cloning kit (Takara).
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bioRxiv - Biochemistry 2022Quote: cDNA fragment of Gtsf1 was amplified by RT-PCR from BmN4 total RNAs and cloned into pIExZ vector (Izumi et al, 2020) by In-fusion cloning kit (Takara).
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bioRxiv - Biochemistry 2022Quote: A cDNA fragment of Gtsf1L was amplified by RT-PCR from BmN4 total RNAs and cloned into pIZ vector by In-fusion cloning kit (Takara).
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bioRxiv - Cell Biology 2022Quote: ... a polymerase chain reaction using 5’-TCTAGAGCTACTAACTTCAGCCTGCTG-3’ / 5’ - CGGTGGATCCCCTTCTTCC-3’ primers on Ibidi USA 60101 LifeAct-GFPtag2 plasmid was cloned with In-Fusion HD enzyme kit (Takara) into the pLVX vector (Clontech ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The cDNA samples were cloned into a pUC19 vector (PCR-amplified with primers 11 and 12) using In-Fusion HD Cloning Kit (Takara). After transformation into Escherichia coli ...
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bioRxiv - Cell Biology 2022Quote: ... Full-length and N-terminally deleted versions of HaloTag-hCAP-H vectors were constructed by fusing the 3’ ends of the corresponding cDNAs with a HaloTag fragment using In-fusion HD Cloning Kit (TaKaRa). The following primers were used the construction of these HaloTag-hCAP-H vectors ...
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bioRxiv - Cell Biology 2022Quote: ... Deletion of the predicted helix motif in the hCAP-H N-tail was performed using PrimeSTAR Mutagenesis Basal Kit (TaKaRa). Primers used in the deletion were as follows ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was quantified using Naodrop and 1μg RNA was used for cDNA synthesis using commercial cDNA preparatory kit (Takara 6110A). For gene analysis the cDNA was amplified using gene specific primers by qPCR with Takara 2X SYBR Green Mix (Takara RR420A ...
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bioRxiv - Immunology 2022Quote: ... All resulting RNA was used as an input for complementary DNA synthesis using the SMART-Seq v4 kit (Takara Bio) and 10 cycles of PCR amplification ...
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bioRxiv - Genetics 2022Quote: ... total RNA extraction and separation of residual DNA contamination were excuted using Plant RNA Extraction Kit (TaKaRa MiniBEST, kyoto, Japan), and RNase-free DNase I (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2022Quote: ... The cloNAT cassette was amplified (LJ42 and LJ43) and inserted into the XhoI site in front of the Nse3 gene using the In-fusion cloning kit (Takara). A 650bp-long genomic sequence (upstream of Nse3 ...
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bioRxiv - Immunology 2022Quote: 150 tuft cells were sorted as CD45lo EpCAM+SigF+CD24+ directly into lysis buffer from the SMART-Seq v4 Ultra Low Input RNA Kit (Takara) and cDNA was generated following manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and cloned into SacI- and Xho-digested pGL3-Promoter vector using the Infusion®HD Cloning kit (Takara Bio #639650). Elements were cloned upstream of the sv40 promoter within the pGL3-Promoter vector ...
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bioRxiv - Microbiology 2022Quote: ... The amplified fragment was cloned into the BamHI site of pET-21a(+) using In-Fusion HD cloning Kit (Takara Bio), and the NotI fragment of the resulting plasmid was then inserted in pJB861 to generate pJBacv ...
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bioRxiv - Microbiology 2022Quote: ... total RNA underwent reverse transcription using a PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) (RR047A, Takara). The Bio-Rad iQ5 system was used to perform a qPCR procedure with the Hieff® qPCR SYBR Green Master Mix (No Rox ...
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bioRxiv - Immunology 2022Quote: ... selected RNAs from cDCs were used to amplify the cDNA using the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech-Takara). Amplified (1 ng ...
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bioRxiv - Immunology 2022Quote: ... Libraries for mRNA sequencing were prepared starting from 10 ng tot RNA for each sample by using the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech-Takara). All samples were sequenced on an Illumina NextSeq 550 at an average of 17,5 million 75-bp single-end reads.
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bioRxiv - Molecular Biology 2022Quote: Y1H library screening was performed by using the Matchmaker Gold Yeast One-Hybrid Library and Screening kit (Clontech,CA, USA). The promoter fragments of CaPR1 were inserted in the pAbAi vectors ...
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bioRxiv - Systems Biology 2022Quote: ... cDNA was synthesized from 1 μg of total RNA using the PrimeScript RT reagent kit (Perfect Real Time) (Cat No RR037A, TaKaRa) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... First-strand cDNA was synthesized from 1 μg of total RNA using the PrimeScript™ RT Master Mix Kit (Takara). SYBR Premix Ex Taq™ kit (Takara ...
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bioRxiv - Plant Biology 2022Quote: ... pOsSHR2::OsSHR2 or pOsPIN5c::OsPIN5c and inserted into the KpnI digested vector pCam23A (Sun et al., 2016) backbone by using the In-Fusion kit (Clontech), with primers are listed in Supplementary Table 4.
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bioRxiv - Molecular Biology 2023Quote: ... The ORF of zebrafish znf598 (ENSDARG00000014945) was amplified by RT-PCR and cloned into pCS2+ via XhoI/XbaI restriction sites using DNA Ligation Kit (TAKARA). To generate znf598 point mutation in ORF (znf598 C13/16A) ...
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bioRxiv - Molecular Biology 2023Quote: ... wild-type ORF was amplified using point mutated primers and cloned into pCS2+ via XhoI/XbaI restriction sites using DNA Ligation Kit (TAKARA). The ORFs of zebrafish etf1b (ENSDARG00000043976 ...
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bioRxiv - Molecular Biology 2023Quote: ... The ORF of zebrafish rpl36 (ENSDARG00000100588) was amplified by RT-PCR and cloned into pCS2+ via EcoRI/XhoI restriction sites using DNA Ligation Kit (TAKARA). The ORF of zebrafish znf598 (ENSDARG00000014945 ...
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bioRxiv - Plant Biology 2023Quote: ... and converted to cDNA using the SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing (Takara Bio), following the manufacturer’s instruction ...
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bioRxiv - Plant Biology 2022Quote: ... which was then cloned into the Aor51HI site in pUGW2 35S 97 using the In-Fusion cloning kit (TaKaRa Bio). The 2.5-kb HindIII-SacI fragment in the resulting plasmid ...
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bioRxiv - Microbiology 2023Quote: ... The resultant fragments were cloned into the EcoRI and NotI recognition sites of pCAG neo using HD Cloning Kit (TaKaRa). All recombinant proteins were fused with a his-tag and expression was confirmed using an anti-his tag antibody.
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bioRxiv - Microbiology 2023Quote: ... and were quantified by real-time RT-PCR using One Step TB Green PrimeScript RT-PCR Kit II (Perfect Real Time) (TaKaRa) and QuantStudio 3 Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... cDNAs were prepared from 1 ng of total RNA using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (TaKaRa) according to the manufacturer guidelines for cDNA synthesis and amplification ...
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bioRxiv - Microbiology 2022Quote: ... The cDNA was acquired by using the Prime Script RT reagent kit with genomic DNA (gDNA) eraser (TaKaRa, Beijing, China). The SYBR Premix Ex Taq II kit (TaKaRa ...
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bioRxiv - Neuroscience 2023Quote: 10 ng RNA from each worm sample was used as input for cDNA synthesis using a SMART-Seq v4 Ultra Low Input RNA kit (Takara). Sequencing libraries were prepared from 500 pg of cDNA with a Nextera XT DNA Library Prep kit (Illumina) ...