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Citations for Takara Bio :
4451 - 4500 of 6263 citations for Arg8 Vasopressin AVP ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... It was subsequently cloned into BamHI site in the pLenti CMV-IRES-GFP by In-Fusion cloning kit (Clontech, 639649) to generate lentiviral packaging plasmids(pLentiCMV-Y437H-IRES-GFP) ...
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bioRxiv - Microbiology 2020Quote: ... The 1.5 kb PCR product was isolated and purified from agarose gel using the NucleoSpin Gel and PCR Clean-up kit (Takara). The product was subjected to Sanger dideoxy sequencing at GENEWIZ (San Diego ...
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bioRxiv - Microbiology 2020Quote: ... Total protein was extracted from 2,000 midguts using the lysis buffer supplied in the Capturem IP & Co-IP kit (Takara, 635721). The extracted proteins were divided into two equal parts and used for immunoprecipitation (IP) ...
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bioRxiv - Biochemistry 2021Quote: ... Lep system including the sequences of interest in the P2 region were subcloned into KpnI linearized pCAGGS in-house version using In-Fusion HD cloning Kit (Takara) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: Whole Genome Amplification (WGA) was performed on 300 single cells for each Time by means of SMARTer PicoPLEX Single Cell WGA kit (Takara) following manufacturer’s protocol as previously described24 ...
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bioRxiv - Cancer Biology 2020Quote: ... RNAseq libraries were generated using total RNA as input with the SMART-Seq V4 Ultra Low Input RNA kit (Clontech). The cDNA libraries were confirmed on the Bioanalyzer using the High Sensitivity DNA kit (Agilent) ...
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bioRxiv - Cancer Biology 2020Quote: Transcriptome sequencing on low input total RNA samples (up to 10 ng) was carried out using SMART-Seq v4 Ultra Low Input RNA Kit (Clontech), according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Lentiviral vectors expressing KDELR2 or a control empty vector were titered using the Lenti-X p24 rapid titer kit (Takara). 8 × 105 SH-SY5Y in 2 mL growth media were plated in a 6-well plate ...
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bioRxiv - Cell Biology 2021Quote: ... RT-PCR was performed using the One Step TB Green PrimeScript PLUS RT-PCR Kit (Perfect Real Time; Takara, Japan) and Thermal Cycler Dice® Real Time System Lite (TP700 ...
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bioRxiv - Cell Biology 2020Quote: ... The purified fragment was cloned into BamHI-SalI site of pLenti-PGK-GFP(34) with In-Fusion cloning HD kit (TAKARA). HEK293T cells were transfected with the pLenti-PGK-Tacc3 or pLKO.1 TRC (control ...
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bioRxiv - Biophysics 2020Quote: Target DNA containing the 5′-TTTA-3′ PAM was ordered from IDT and cloned into a pET28-MHL vector using the In-Fusion Cloning Kit (ClonTech). Plasmids were linearized before usage ...
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bioRxiv - Cell Biology 2020Quote: ... 1 μg of total RNA from each condition was converted into cDNA using PrimeScript RT reagent with gDNA Eraser kit (Takara Clontech) and MJ Research PTC-220 Dyad PCR System (Conquer Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... Libraries for RNA-sequencing were prepared using the SMARTer Stranded Total RNA-Seq Kit v2 Pico Input Mammalian library preparation protocol (Takara Bio USA ...
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bioRxiv - Immunology 2022Quote: Cell death was measured by quantification of the lactate dehydrogenase (LDH) release into the cell supernatant using LDH Cytotoxicity Detection Kit (Takara). Briefly ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was isolated using the TRIzol method and the expression of miRNAs analyzed by qRT-PCR with the Mir-X miRNA qRT-PCR TB Green kit (Takara Bio Inc ...
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bioRxiv - Microbiology 2022Quote: ... The viral RNA copy number was standardized with a SARS-CoV-2 direct detection RT-qPCR kit (Takara, Cat# RC300A). Fluorescent signals were acquired using QuantStudio 3 Real-Time PCR system (Thermo Fisher Scientific) ...
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bioRxiv - Physiology 2022Quote: ... cDNA was generated and amplified using the Smart-SEQ HT Kit for low RNA input according to the manufacturer’s recommendations (Takara Bio), which was used for generating sequencing libraries with the Nextera XT kit ...
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bioRxiv - Cell Biology 2022Quote: ... and LSK+ or LSK- RNA-seq libraries were prepared using SMART-Seq v4 Ultra Low Input RNA Kit (Takara, 634888) and KAPA HyperPrep kit (KAPA ...
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bioRxiv - Biochemistry 2022Quote: ... cDNA synthesis was carried out using the Primescript™ RT reagent kit with gDNA eraser (perfect real time) (TaKaRa, JP) following the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2022Quote: Trophectoderm biopsies containing 5-10 cells from blastocyst-stage embryos (n=24) were processed for RNA-seq using a commercial kit (Takara Bio ...
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bioRxiv - Molecular Biology 2022Quote: ... First-strand cDNA was synthesized from the total RNA using a PrimeScript™ RT Reagent Kit with gDNA Eraser (Takara) following the manufacturer’s protocols ...
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bioRxiv - Immunology 2022Quote: ... – PU6-pAzpa was co-transfected with plasmids expressing full-lengths mutant 2B4 TCR and CD3 subunits using Xfect transfection kit (TaKaRa). For incorporating pAzpa into TCR sites ...
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bioRxiv - Plant Biology 2022Quote: ... PCR fragments representing either the OsSOG1 promoter+GUS gene or OsSGL promoter+GUS gene were inserted into the AscI/AvrII site using an In-Fusion HD cloning kit (Takara) between attL1 and T35S in pE(L1-L2)hpt ...
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bioRxiv - Microbiology 2022Quote: ... Then RNA was reverse-transcribed and amplified using One Step PrimeScriptTM III RT-qPCR Mix kit (Takara Bio, Kyoto, Japan). Primers and probes ...
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bioRxiv - Genetics 2022Quote: ... and 2 ng of total RNA was amplified with SMART-Seq v4 Ultra Low Input RNA kit (Clontech; version “091817”). Sequencing libraries were prepared with NEBnext Ultra DNA Library Prep Kit (New England Biolabs ...
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bioRxiv - Cell Biology 2022Quote: ... reverse transcription and cDNA synthesis were performed using the SMART-Seq v4 Ultra Low RNA Kit for the Fluidigm C1 System (Takara) reagents ...
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bioRxiv - Cell Biology 2022Quote: ... For each specimen 500 ng of total RNA was reversed transcribed into cDNA using PrimeScript RT reagent kit (TaKaRa RR037a) and oligo dT primers according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... PtoATPE and PtoPSBB) were co-transformed into the Y1HGold yeast strain using the Matchmaker One-Hybrid Library Construction and Screening Kit (Clontech), respectively ...
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bioRxiv - Microbiology 2022Quote: ... One μg of total RNA was used as input for SMARTer Stranded Total RNA Sample Prep Kit-HI Mammalian (Clontech). Sequencing was performed on the NextSeq500 instrument (Illumina ...
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bioRxiv - Molecular Biology 2022Quote: ... Ribo-depleted sequencing libraries were prepared with the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (TaKaRa Biotech) following manufacturer instructions and sequenced with Genewiz/Azenta on an Illumina NovaSeq S4 machine using a 2 x 150 bp pair-end read configuration to a minimum depth of 30 million reads per sample ...
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bioRxiv - Biophysics 2022Quote: ... 5’ and 3’-RACE-Ready cDNA templates were synthesized using a SMARTer® RACE 5’/3’ Kit (Takara Bio, USA) and subsequently used to amplify 5’ and 3’ end sequences of P ...
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bioRxiv - Bioengineering 2022Quote: ... the gel-purified RNA was reverse transcribed into cDNA using a PrimeScript RT Reagent Kit with random primers (TAKARA, RR037B), followed by PCR with primers that can amplify transcripts across the splice junction ...
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bioRxiv - Bioengineering 2022Quote: The cells were confirmed to be mycoplasma-negative every few months using a CycleavePCR Mycoplasma Detection Kit (CY232, Takara Bio).
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bioRxiv - Cell Biology 2022Quote: ... and inserted between SpeI and NotI restriction sites (pFastBac1-hCAP-D3-[3C]-StrepII) by using restriction enzymes and a DNA ligation kit (TaKaRa). To create the hCAP-H2 construct with an N-terminal StrepII-tag (pFastBac-StrepII-[3C]-hCAP-H2) ...
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bioRxiv - Developmental Biology 2022Quote: ... Single-cell cDNAs were prepared on Fluidigm C1 system by using SMARTer v4 Ultra Input Low RNA kit for the Fluidigm C1 System (Clontech). Only single-cell cDNAs confirmed by imaging data were performed quality check (QC ...
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bioRxiv - Developmental Biology 2022Quote: ... the maximuM amount (10.5 µl) was used for library preparation using the SMART-Seq HT kit (Takara, Cat. No. 634437) followed by Nextera XT DNA Library Preparation Kit (Illumina ...
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bioRxiv - Cell Biology 2022Quote: ... full length double-stranded cDNA was prepared from 1 ng of total RNA according to the manufacturer’s instructions for the SMART-Seq v4 Ultra Low Input RNA Kit® (Clontech). Quantity and quality of the amplified full length double-stranded cDNA were assessed using both Qubit (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... RNA-Seq libraries were generated with the SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian (v1 or v2; Takara) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... DNAs of TOMM20-N-10-CLIPf and SNAPf-Clathrin-15 were directly PCR amplified from mammalian expression constructs and subcloned into lentiviral pHR-SFFV vector (BamHI/NotI) using In-Fusion HD Cloning kit (Clontech).
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bioRxiv - Neuroscience 2020Quote: ... Total RNA was extracted from the isolated tissue and was subjected to reverse transcription (RT) with the use of a NucleoSpin RNA extraction kit (U955C, Takara) and PrimeScript RT reagent kit (RR037 ...
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bioRxiv - Neuroscience 2020Quote: ... oligo(dT)-enriched RNA underwent 14 cycles of amplification using the SMARTer Ultra Low RNA Kit for Illumina Sequencing (Clontech). After cDNA fragmentation ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA libraries were produced using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara 634894, Lot 1709695A) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: 500 ng of the extracted total RNA were subjected to the first-strand cDNA synthesis with the primescript RT reagent kit (Takara). Quantitative real-time PCR was performed with Thunderbird SYBR qPCR mix (Toyobo ...
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bioRxiv - Plant Biology 2021Quote: The Pro197Ser-mutated ALS genes and the native form of MvALS1 gene were inserted into the pCAMBIA1390 vector under the cauliflower mosaic virus 35S promoter using the In-Fusion DH Cloning Kit (TaKaRa), as described previously (Iwakami et al. ...
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bioRxiv - Plant Biology 2021Quote: ... after-ripened Col and ref6c seeds collected at different imbibition time points were subjected for total RNA extraction using Minibest plant RNA extraction kit (Takara). Three independent biological replicates were performed ...
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bioRxiv - Plant Biology 2021Quote: ... The amplicons from SlACS2-prom1k-pPLV-F/R were cloned into pPLV451 using an In-Fusion HD Cloning kit (Clontech), to construct pACS2-GFPx3 and pACS2mut-GFPx3 ...
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bioRxiv - Microbiology 2020Quote: ... gene was then PCR amplified and purified then cloned into plasmid pLC29144 using In-Fusion HD Cloning plus kit (Takara). All PCR products and linearized vector were purified (Macherey and Nagel ...
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bioRxiv - Microbiology 2020Quote: ... overhangs upstream and downstream of the gene of interest were amplified (primers sequences available in Table S1) and purified then cloned into linearized pCM433 using In-Fusion HD Cloning plus kit (Takara), primer design was done using primer design tool (Takara) ...
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bioRxiv - Developmental Biology 2021Quote: ... Libraries were prepared from 50 ng of total RNA/sample as input using Ribogone and a SMARTer Universal Low Input RNA Kit (Clontech). For inDrops ...
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bioRxiv - Genomics 2019Quote: ... The IsoSeq libraries were created starting from 1µg of total RNA per sample and full-length cDNA was then generated using the SMARTer PCR cDNA synthesis kit (Clontech) following PacBio recommendations set out in the IsoSeq method (pacb.com/wp-content/uploads/Procedure-Checklist-Iso-Seq-Template-Preparation-for-Sequel-Systems.pdf) ...