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Citations for Takara Bio :
401 - 450 of 1000 citations for Mouse Anti Nipah Virus Glycoprotein G AE6 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... the transfer plasmid (pBABEpuro-based or pLZRS-IRES-ΔNGFR/GFP-based) and pCMV-VSV-G were co-transfected into GP2-293 packaging cells using CalPhos mammalian transfection kit (Takara/Clontech) according to manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was collected after centrifugation at 65,000 g for 35 min and was incubated with TALON® Metal Affinity Resin (TaKaRa) for 2 h at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... The non-solubilized fraction was separated by centrifugation at 47850 x g for 1 h at 4 °C and 4 mL of cobalt-charged TALON resin (Clontech, Takara) was added to the solubilized fraction for batch binding (3 h at 4 °C) ...
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bioRxiv - Cancer Biology 2024Quote: ... with psPAX2 and pMD2.G viral particles harvested during the 16-72 h window post-transfection were concentrated with Lenti-X (Clontech) and stored at -80°C ...
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bioRxiv - Cell Biology 2020Quote: Mouse monoclonal E-cadherin antibody (HECD-1) was purchased from Takara Bio Inc ...
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bioRxiv - Genetics 2019Quote: ... GAL4 DNA-BD Mouse Monoclonal Antibody (Takara Bio USA, Inc. #630403), and GAPDH Mouse Monoclonal Antibody (CB1001-500UG 6C5 ...
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bioRxiv - Microbiology 2021Quote: ... Gal4-based two-hybrid screening against the mouse cDNA library (Clontech) was performed as described before (Mitsuzawa et al. ...
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bioRxiv - Developmental Biology 2022Quote: ... Predicted enhancer elements were PCR-amplified from mouse genomic DNA (Clontech) and cloned into the respective LacZ expression vector (Osterwalder et al ...
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bioRxiv - Bioengineering 2022Quote: ... EGFP was detected using the JL-8 mouse monoclonal antibody (Clontech). For IHC ...
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bioRxiv - Neuroscience 2023Quote: ... primary mouse cortical neurons were transfected with pEGFP-C1 vector (Clontech) at 3 days in vitro (DIV ...
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bioRxiv - Cell Biology 2023Quote: ... A cDNA library generated from mouse placenta total mRNA (Takara, 636672) served as template to amplify the domains by PCR ...
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A gene desert required for regulatory control of pleiotropic Shox2 expression and embryonic survivalbioRxiv - Developmental Biology 2023Quote: ... Predicted enhancer elements were PCR-amplified from mouse genomic DNA (Clontech) and cloned into a Hsp68-LacZ expression vector for random integration114 ...
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bioRxiv - Plant Biology 2020Quote: ... About 2 μg of the isolated RNA was used to synthesize the first-strand cDNA using reverse transcriptase enzyme (Takara Bio, Clontech, USA). The cDNA was diluted seven times with sterile Milli-Q water (1:7 ratio) ...
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bioRxiv - Cancer Biology 2020Quote: ... 1 µg of total RNA served as a template for cDNA synthesis with a PrimeScript™ 1st strand cDNA Synthesis Kit (Takara). GAPDH and 18S ribosomal RNA were amplified as reference genes for target mRNA ...
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bioRxiv - Cell Biology 2021Quote: ... lentiviral shRNA expression constructs were transfected together with packaging vectors (VSV-G, RSV-Rev and GAG-Pol) into 293T producer cells using CalPhos Transfection Kits (Takara Bio) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... 1 μg of total RNA from each condition was converted into cDNA using PrimeScript RT reagent with gDNA Eraser kit (Takara Clontech) and MJ Research PTC-220 Dyad PCR System (Conquer Scientific) ...
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bioRxiv - Immunology 2022Quote: ... The g block and linearized plasmid backbone were ligated together using the In-fusion HD cloning kit (Takara Bio, catalog #638920).
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bioRxiv - Microbiology 2021Quote: ... 1 μg of RNA was used for library preparation with the SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian (Takara Bio), following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... These were then packaged into lentivirus via cotransfection with CMV-Gag-Pol (Harvard #dR8.91) and pVSV-G (Clontech, part of #631530) plasmids into production HEK293T cells adapted to grow in serum-free conditions (OHN media ...
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bioRxiv - Neuroscience 2020Quote: ... pHR-CNIH2-ID4-HA was co-transfected with psPAX2 and pMD2.G into HEK293T Lenti-X cells (Takara/Clontech, Cat# 632180). 72 hours after transfection ...
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bioRxiv - Neuroscience 2020Quote: ... pHR-CNIH2-ID4-HA was co-transfected with psPAX2 and pMD2.G into HEK293T Lenti-X cells (Takara/Clontech, Cat# 632180). 72 hours after transfection ...
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bioRxiv - Physiology 2022Quote: ... NS was induced by intraperitoneal injection of 0.5 μg/g body weight of the chemical AP20187 (Takara Bio Inc. Kusatsu, Japan), in 8-10 weeks-old male transgenic mice ...
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bioRxiv - Genomics 2020Quote: ... The cDNA synthesis was carried out by using 5 g of total RNA or 1 g of PAPed RNA with RT primer (5-TTTTTTTTUUUTTTTTVN-3) by PrimeScript II Reverse Transcriptase (TaKaRa Bio). The full-length cDNAs were selected by Cap Trapper method 60 ...
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bioRxiv - Immunology 2021Quote: ... The retroviral transfer vector was co-transfected with pVSV-G retroviral envelope-expressing plasmid into the GP2-293 cell line (Clontech-TaKaRa) using Lipofectamine 2000 reagent (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... packaging (pCAG-HIVgp) and envelope (pCMV-VSV-G-RSV-Rev) plasmids were co-transfected into Lenti-X 293T cells (Takara Bio) using Fugene 6 reagent (Promega) ...
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bioRxiv - Microbiology 2022Quote: ... The resultant lysate was cleared by centrifugation at 30 000 x g for 20 min and applied to Talon CellThru Co2+ chelating resin (TaKaRa-Clontech). The protein bound resin was washed with 3 column volumes (CV ...
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bioRxiv - Plant Biology 2022Quote: ... 1 μg of total RNA was used to synthesize first-strand cDNA with PrimeScript RT enzyme together with gDNA eraser (Takara, Japan). qRT□PCR was performed on a Roche LightCycler 480 (Roche Diagnostics ...
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Ferroportin Depletes Iron Needed for Cell Cycle Progression in Head and Neck Squamous Cell CarcinomabioRxiv - Cancer Biology 2022Quote: Viral particles were created via co-transfection of the pLVX-tet-FPN vector and 4th generation packaging vectors using the Lenti-X packaging Single Shots (VSV-G) (Takara Bio) in Lenti-X HEK 293T cells ...
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bioRxiv - Microbiology 2023Quote: ... 293T cells were cotransfected with 2.4 μg of proviral DNA construct and 0.6 μg of VSV-G expression vector using TransIT-LT1 reagent (Takara, Cat# MIR2306) into 293T cells (3 × 106) ...
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bioRxiv - Neuroscience 2023Quote: ... The non-solubilized fraction was separated by centrifugation at 47850 x g for 1 h at 4 °C and 4 mL of cobalt-charged TALON resin (Clontech, Takara) was added to the solubilized fraction for batch binding (3 h at 4 °C) ...
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bioRxiv - Plant Biology 2023Quote: ... Each sample (2 μg) of total RNA was used to synthesize first-strand cDNA with a PrimeScript RT Kit with gDNA eraser (Takara, RR0447A). RT-qPCR was performed using a Roche LightCycler 480 system with a SYBR Fast qPCR Kit (KAPA Biosystems ...
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bioRxiv - Neuroscience 2023Quote: ... We then synthesized these sequences as G-blocks (Integrated DNA Technologies) and cloned them using Infusion kit (Takara biosciences catalog # 638948) into pSMART-HC-Kan (Lucigen ...
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bioRxiv - Cancer Biology 2023Quote: ... 293T cells were transfected with each of the above-mentioned vectors and Lenti-X Packaging Single Shots (VSV-G) (Clontech, 631275) in the presence of reduced-serum Opti-MEM medium (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... the transfer plasmid (pBABEpuro-based or pLZRS-IRES-ΔNGFR/GFP-based) and pCMV-VSV-G were co-transfected into GP2-293 packaging cells using CalPhos mammalian transfection kit (Takara/Clontech) according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... lysates were centrifuged at 31,000 x g for 45 min at 4°C and the supernatant was mixed with nickel-nitrilotriacetic acid (Ni-NTA) beads (Takara, 635653) at 4°C for 2 h ...
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bioRxiv - Cell Biology 2021Quote: ... anti-HA (Clontech), anti-GST (Santa Cruz Biotechnology) ...
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bioRxiv - Cell Biology 2020Quote: ... anti-STEM121 (Takara; Dilution 1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... vector used was AAV9-CaMKII::G-CaMP7 (9.4 × 1012 vg/mL)35 after 10-fold dilution with phosphate buffered saline (PBS) (T900, Takara BIO INC, Japan) before injection ...
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bioRxiv - Neuroscience 2021Quote: Auto-TDP-43-HA was then packaged into lentivirus (LV) via co-transfection with CMV-Gag-Pol (Harvard #dR8.91) and pVSV-G (Clontech, part of #631530) plasmids into production HEK293T cells adapted to grow in serum-free conditions (OHN media ...
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bioRxiv - Neuroscience 2022Quote: ... vector encoding AAV9-CaMKII::G-CaMP7 (Titer: 9.4 × 1012 vg/mL) after 40- fold dilution with phosphate buffered saline (PBS) (T900; Takara Bio, Inc., Japan) was used41 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Total RNA was extracted using Sepasol-RNA I Super G (Nacalai Tesque) according to the manufacturer’s instructions and reverse-transcribed using PrimeScript Reverse Transcriptase (Takara Bio, Shiga, Japan). Expression of cytokines was quantified using a Thermal Cycler Dice Real Time System (Takara Bio ...
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bioRxiv - Genomics 2021Quote: ... Purified RNA samples were diluted to 1 μg/μL and pooled to perform cDNA synthesis by utilizing PrimerScript first strand cDNA synthesis kit (Cat. no. 6110A, Takara Bio, Japan), following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... Gaillardia × grandiflora ‘Arizona Apricot’ and Antirrhinum majus using Sepasol RNA I Super G (Nacalai Tesque) and purified with a high-salt solution for precipitation (Takara Bio Inc.). A five-micro gram of total RNA was used for the gel blot analysis ...
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bioRxiv - Immunology 2023Quote: ... Plasmids were packed into lentiviral particles using the Lenti-X cell line and Lenti-X Packaging Single Shots (VSV-G) (Takara, Cat.No. 631276) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... The crude lysates were centrifuged at 35’000 g for 30 min and the cleared lysates incubated with 200 μL Ni-NTA agarose beads (Takara Bio, Shiga, Japan) for 1h at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... Lentivirus-containing conditioned media were centrifuged at 500 x g for 5 min at 4°C to remove cellular debris and concentrated using Takara LentiX (Takara Cat# 631231). Briefly ...
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bioRxiv - Immunology 2022Quote: ... The SMARTer Mouse TCR a/b Profiling Kit (Takara, Cat. No. 634403) was used to amplify both TCRα and TCRβ sequences ...
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bioRxiv - Cell Biology 2024Quote: ... The mouse PG cDNA was generated by PrimeSTAR Max DNA Polymerase (Takara) to skip 150 bp ...
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bioRxiv - Physiology 2022Quote: ... Kidney fibrosis was induced by one daily intraperitoneal injection of 0.2 μg/g body weight for 5 days of the chemical AP20187 (Takara Bio Inc. Kusatsu, Japan), in 8-10 weeks-old male transgenic mice ...
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bioRxiv - Molecular Biology 2021Quote: ... One µg of total RNAs was subjected to reverse transcription into cDNAs using PrimeScriptTM RT reagent Kit with gDNA Eraser (Takara, cat. no. RR047A) according to manufacturer’s protocol ...