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Citations for Takara Bio :
401 - 450 of 5010 citations for Glutathione Colorimetric Detection Kit 4 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... A tomato Mate & Plate library was constructed and used for screening with the Matchmaker Gold Yeast Two-Hybrid System (Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: iPSCs SFCi55 and hESCs RUNX1-GFP were plated at 3 × 105 cells per a well of a 6 well plate and reverse transfected with 2 μg of DNA using the Xfect Transfection reagent (Clontech) and analyzed 2 days later.
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bioRxiv - Immunology 2022Quote: ... Lentiviral supernatants were spun onto nontreated wells plates coated with 30 μg/mL Retronectin reused up to three times (T100B, Takara), at 2000 x g for 2 hours at 32°C and replaced with activated human CD8+ T cells in fresh RPMI supplemented with 30 U/mL IL-2.
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bioRxiv - Plant Biology 2022Quote: ... which was conducted with the “Make Your Own “Mate & Plate™” Library System” according to the manufacturer’s instructions with minor modifications (Clontech). Oligo-dT primers provided in the kit were used for first-strand cDNA synthesis with 2 µg total RNA as input ...
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FTSH3 facilitates complex I degradation through a direct interaction with the complex I subunit PSSTbioRxiv - Plant Biology 2023Quote: ... Mating was carried out in a 96-well plate according to manufacturer’s instructions (Matchmaker GAL4 Two Hybrid System (Clontech/TakaraBio). Mated diploid strains were serially diluted and plated on - Leu–Trp double dropout (DDO ...
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bioRxiv - Plant Biology 2023Quote: ... The cDNA library was prepared from rice flowers and ligated to the pGADT7-Rec vector using the Make Your Own “Mate and Plate” Library System (Takara). A second cDNA library was prepared from rice leaves under salt stress ...
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bioRxiv - Neuroscience 2023Quote: ... and AAV1-syn-F14F15S-sTpEptTA_v2 were made by transfecting 8×15c plates (per virus) of HEK 293T cells using Xfect transfection reagent (Takara 631318). Briefly ...
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Induction of PARP7 Creates a Vulnerability for Growth Inhibition by RBN2397 in Prostate Cancer CellsbioRxiv - Cancer Biology 2023Quote: ... BBQ and FICZ as indicated at 37°C in 96-well plates for 5 days prior to measuring viability using WST reagent (Takara) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... and GC B cells (live singlet CD19+ CD4− IgDlo CD71+CD38int CD20+ CXCR5+) were sorted using a FACSAria II into 96-well plates containing 2 μL Lysis Buffer (Clontech) supplemented with 1 U μl−1 RNase inhibitor (NEB) ...
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bioRxiv - Cell Biology 2023Quote: ... Fractionated CD34+ cells from Animals #2 and #3 were cultured overnight on RetroNectin-coated plates (Takara, T100B, Mountain View, CA) in X-VIVOTM 10 (Lonza ...
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bioRxiv - Molecular Biology 2023Quote: Lentivirus was produced using a standard protocol64 from a 15 cm plate of 25M lenti-x 293T cells (Clontech #632180) via polyethylenimine (PEI ...
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bioRxiv - Cancer Biology 2023Quote: ... 150,000 cells were seeded in 6-well plates 1 day before transfection with 1 μg pp53-TA-Luc vector (Clontech) and 0.015 μg pRL-CMV-Renilla (Promega ...
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bioRxiv - Cell Biology 2024Quote: iPSCs SFCi55 and hESCs RUNX1-GFP were plated at 3 × 105 cells per a well of a 6 well plate and reverse transfected with 2 μg of DNA using the Xfect Transfection reagent (Clontech) and analyzed 2 days later.
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bioRxiv - Genetics 2024Quote: ... 0.2*106 mESCs were seeded in a well of a 12-well plate in 2iL and transduced the next day with 1ml of 5x concentrated (lenti-X, Clontech) and filtered viral supernatant with 8 ng/μl polybrene (Sigma Aldrich) ...
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bioRxiv - Cancer Biology 2024Quote: ... Meanwhile, untreated 6-well cell culture plates (Falcon, 351146) were coated with 15 µg/ml retronectin solution (Takara Bio, T100A) overnight at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... approximately 20% confluent HEK293T cells seeded in 12-wells plates were transfected with 300ng ZNRF3 encoding plasmid and 50ng GFP expression plasmid (pEGFP-C1, Clontech) using Lipofectamine 2000 ...
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bioRxiv - Plant Biology 2023Quote: ... the Y2HGold strain was transformed with the bait vector (pGBKT7-SR45) and then mated with strain Y187 containing an Arabidopsis cDNA library (Mate and Plate Library-Universal Arabidopsis, Clontech) (Stankovic et al. ...
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bioRxiv - Immunology 2019Quote: ... Viral titers were measured by a kit (Lenti-X p24 Rapid titer kit, #632200. Takara, Kusatsu, Japan). To suppress Mul1 expression ...
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bioRxiv - Neuroscience 2020Quote: ... and reverse transcribed using complementary DNA (cDNA) synthesis kit (PrimeScript 1st strand cDNA Synthesis Kit, Takara Bio). The synthesized cDNA was subjected to qPCR using KAPA SYBR (KAPABiosystems ...
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bioRxiv - Neuroscience 2020Quote: ... Viral titer was determined using a qPCR Lentivirus Titration Kit (Lenti-X, qRT-PCR Titration Kit, Takara). For smaller scale virus preparation ...
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bioRxiv - Neuroscience 2020Quote: ... in a 100 mm dish using a calcium phosphate transfection kit (CalPhos Mammalian Transfection kit, Clontech, 631312). Two days post-transfection ...
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bioRxiv - Synthetic Biology 2022Quote: ... The kit used for qPCR experiments was PrimeScript™ II 1st Strand cDNA Synthesis Kit (TaKaRa, Japan), HiScriptII 1st Strand cDNA Synthesis Kit (Vazyme Biotech Co. ...
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bioRxiv - Developmental Biology 2021Quote: ... was incubated with antibodies at 4°C for one hour: 3 µl (0.5 µg/µl) c-Myc monoclonal antibody (Cat. No. 631206, TaKaRa), or 1 µl (1.1 µg/µl ...
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bioRxiv - Neuroscience 2020Quote: ... before addition of 4 mM imidazole and incubation on a rotor (111 RPM) with TALON metal affinity resin (Takara, Cat.# 635503 ...
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bioRxiv - Biochemistry 2021Quote: ... The cell lysate was centrifuged at 40,000 × g for 45 min at 4 °C and the supernatant was loaded onto a cobalt affinity column (Clontech). After washing the column with 20 bed volume of 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... The lysate was clarified at 26,915 x g for 50 minutes at 4°C and then incubated with TALON metal affinity resin (Clontech). His-Nsp15 was eluted from the resin with 250 mM imidazole ...
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bioRxiv - Microbiology 2020Quote: ... 4 μL of 10 × PCR Buffer (Mg2+ plus) provided with the TAKARA Taq™ Hot Start Version (TAKARA, Japan), 0.08 μL of TransScript® Reverse Transcriptase [M-MLV ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant was clarified by centrifugation (292,055 g, 60 min, 4°C) and bound to 2 ml of TALON IMAC resin (Clontech) overnight with 10 rpm rotation in the presence of 20 mM imidazole and NaCl added up to 800 mM ...
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bioRxiv - Neuroscience 2021Quote: ... followed by an incubation overnight at 4°C with antiDsRed rabbit polyclonal primary antibody (1:1000, 632496 Takara Bio) diluted in a triton buffer (0.3% Triton X-100 diluted in PBS-DEPC) ...
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bioRxiv - Neuroscience 2021Quote: ... followed by an incubation overnight at 4°C with antiDsRed rabbit polyclonal primary antibody (1:1000, 632496 Takara Bio) diluted in a triton buffer (0.3% Triton X-100 diluted in PBS-DEPC) ...
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bioRxiv - Biochemistry 2020Quote: ... The lysate was clarified at 26,915 × g for 50 minutes at 4°C and then incubated with TALON metal affinity resin (Clontech). His-Thrombin-TEV-Nsp15 variants were eluted with 250 mM imidazole and further purified by gel filtration on a Superdex-200 column (GE Healthcare ...
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bioRxiv - Biophysics 2021Quote: ... The resulting supernatant was collected by ultracentrifugation (150,000 × g, 1 hour, 4 °C) and applied onto an immobilized metal-ion affinity chromatography column (Talon, Clontech) equilibrated with 40 mM Tris ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... A plasmid encoding the G protein chimera GqGi3 bearing the core of human Gαq and the last 4 amino acid of Gαi3 was generated by primer mutagenesis and In-Fusion HD Cloning (Clontech) in a pcDNA3.1 vector ...
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bioRxiv - Pathology 2023Quote: ... Cells were harvested by centrifugation at 4,000 rpm for 20 min at 4℃ and resuspended in 20 ml xTractor buffer (Clontech, TaKaRa Biomedical Technology [Beijing] Co. ...
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bioRxiv - Pathology 2023Quote: ... Cells were harvested by centrifugation at 4,000 rpm for 20 min at 4℃ and resuspended in 20 ml xTractor buffer (Clontech, TaKaRa Biomedical Technology [Beijing] Co. ...
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bioRxiv - Developmental Biology 2023Quote: ... They were then incubated O/N at 4°C with the corresponding primary antibody: anti-DsRed (1:500; Clontech), mouse anti-GFP ([1:100] ...
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bioRxiv - Genomics 2022Quote: ... Paris) were prepared following the kit manufacturer’s recommendations (SMART-Seq v4 Ultra Low Input RNA Kit from Takara). Samples were sequenced on a NextSeq 500 Illumina device with MidOutPut cartridge to generate a total of 2 × 87 M reads of 75 bp ...
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bioRxiv - Genetics 2021Quote: ... The qPCR procedure was carried out according to the kit instructions (TB Green Premix Ex Taq kit, TakaRa). Primers used were as follows:
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bioRxiv - Immunology 2020Quote: ... and reverse transcribed using a first-strand cDNA synthesis kit (PrimeScript™ 1st strand cDNA Synthesis Kit, Takara) according to the manufacturer’s protocol ...
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Deletion or inhibition of PTPRO mitigates diet-induced hepatic steatosis and inflammation in obesitybioRxiv - Immunology 2022Quote: ... Protein concentrations were assessed with the BCA protein assay kit (Takara BCA Protein Assay Kit, Takara, Shiga, Japan). SDS-PAGE and Western blotting were performed as previously described (Shintani et al. ...
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bioRxiv - Bioengineering 2022Quote: ... prepared with a SMARTer Stranded Total RNA-Seq Kit v3 – Pico Input Mammalian kit (Takara Bio Inc., Japan) with 0.75 ng and bulk-sequenced using 100 bp paired-end reads at a depth of 50 million reads ...
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bioRxiv - Genomics 2022Quote: ... Libraries were prepared using the SMARTer® Stranded Total RNA-seq Kit v2-Pico Input Mammalian kit (Takara). Two technical replicates were generated for each experiment ...
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bioRxiv - Microbiology 2022Quote: ... Complementary DNA (cDNA) was synthesized using the PrimeScript RT reagent kit (Perfect Real Time Kit, Takara Biochemicals, China). According to the vendor’s instructions ...
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bioRxiv - Neuroscience 2023Quote: Libraries were constructed with the SMARTer® Stranded Total RNA-Seq Kit – Pico Input Mammalian kit (Clontech Laboratories), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: Sample libraries were prepared with SMART-Seq v4 Ultra Low Input RNA Kit (Takara Clontech Kit Cat# 63488). Libraries were sequenced on a NovaSeq 6000 (Illumina ...
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bioRxiv - Developmental Biology 2023Quote: Sample libraries were prepared with SMART-Seq v4 Ultra Low Input RNA Kit (Takara Clontech Kit Cat# 63488). Libraries were sequenced on a NovaSeq 6000 (Illumina ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA-seq libraries were prepared using the SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian kit (Takara) due to retirement of the ScriptSeq v2 kit ...
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bioRxiv - Cell Biology 2024Quote: ... Complementary DNA (cDNA) was synthesized using the PrimeScript™ RT reagent Kit with gDNA Eraser Kit (TaKaRa, Japan) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... Retroviruses encoding a second-generation CAR targeting HER-2 was obtained from the supernatant of the GP+E86 packaging line spun together with T cells onto RetroNectin-coated (10 μg/ml) 6-well plates (Takara Bio) and incubated overnight before the second viral transduction ...
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bioRxiv - Cancer Biology 2021Quote: ... freshly harvested single cells were sorted into 96-well plates containing the lysis buffer (0.2% triton-X-100, 1U/µl RNase inhibitor (Takara Bio, 2313A). Reverse transcription was performed using SuperScript II (ThermoFisher Scientific ...