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Citations for Takara Bio :
401 - 450 of 2247 citations for DNA Mismatch Repair Protein Msh3 MSH3 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2022Quote: ... RNA and DNA were extracted from the larvae using a NucleoSpin RNA XS and a NucleoSpin RNA/DNA Buffer Set (Takara Bio). If individuals were homozygous at two CSD loci (male genotype ...
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bioRxiv - Immunology 2024Quote: ... tag followed by a stop codon at the C-terminal were synthesized (Integrated DNA Technologies) and amplified by PCR using Prime STAR Max DNA polymerase (Takara Bio). The PCR product was purified using a PureLink PCR purification kit (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR on genomic DNA from all replicates was performed using Titanium Taq DNA Polymerase and PCR buffer (Clontech Takara Cat# 639208) according to The Broad Institute Genetic Perturbation Platform’s protocol for PCR of sgRNAs from genomic DNA for Illumina sequencing (https://portals.broadinstitute.org/gpp/public/resources/protocols) ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR on genomic DNA from all replicates was performed using Titanium Taq DNA Polymerase and PCR buffer (Clontech Takara Cat# 639208) according to The Broad Institute Genetic Perturbation Platform’s protocol for PCR of sgRNAs from genomic DNA for Illumina sequencing (https://portals.broadinstitute.org/gpp/public/resources/protocols) ...
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bioRxiv - Synthetic Biology 2021Quote: ... The following antibodies were used: Anti-GFP antibody (JL-8 from Takara, Shiga, Japan), anti-Flag antibody (M2 from Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... Monoclonal Antibody (JL-8) (Clontech). Anti-mouse IgG was used as a secondary antibody.
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bioRxiv - Biochemistry 2022Quote: ... or anti-GFP antibody (Clontech) for 1 hour at room temperature followed by a second incubation with HRP-conjugated secondary antibody (Santa Cruz Biotechnology) ...
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bioRxiv - Cell Biology 2020Quote: ... Equal amounts of protein were incubated with TALON beads (Clontech) pre-equilibrated with purification buffer at RT for 1.5 h with overhead rotation ...
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bioRxiv - Plant Biology 2020Quote: ... the proteins were purified by TALON Metal Affinity Resin (Clontech) and filtered with Amicon Ultra 0.5ml (30K ...
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bioRxiv - Synthetic Biology 2021Quote: ... concentrated proteins were determined those concentrations with Bradford (Takara Bio) or BCA (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... The solubilized protein was purified by Talon-resin (Clontech/Takara) using the hexa-histidine-tag fused at the N-terminus of the protein ...
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bioRxiv - Cell Biology 2020Quote: ... The solubilized protein was purified by Talon-resin (Clontech/Takara) using the hexa-histidine-tag fused at the N-terminus of the protein ...
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bioRxiv - Cell Biology 2021Quote: ... which encodes the chaperone protein tag (TaKaRa Bio, Shiga, Japan), following the manufacturer protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Cyan fluorescent protein (CFP) expression plasmid was pECFP-C1 (Clontech). Plasmids pCAGGS-CD4-Myc (Addgene ...
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bioRxiv - Genomics 2023Quote: ... and tdTomato fluorescent protein using in-Fusion cloning (Takara Bio). The 5-plasmid system includes a packaging vector (pHAGE-H2B-NanoLuc-T2A-tdTomato) ...
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bioRxiv - Cell Biology 2024Quote: ... and protein concentration was estimated by BCA Kit (Takara-#T9300A). For the assay ...
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bioRxiv - Molecular Biology 2023Quote: ... sfGFP-tagged proteins were visualized with monoclonal anti-GFP (Takara). Anti-Sty1 polyclonal antibody (Jara et al ...
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bioRxiv - Biochemistry 2023Quote: ... protein complexes were purified from by Ni2+-NTA (Takara Bio) affinity chromatography (Dsl1:Qb:Qc and His7-Tip20:Sec20 ...
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bioRxiv - Molecular Biology 2024Quote: ... The protein was next cleaved by adding 3C protease (Takara Bio ...
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bioRxiv - Biochemistry 2024Quote: ... Recombinant proteins were purified by His TALON gravity column (Clontech) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Genomic DNA samples from the patients’s paired tumor tissues and WBCs were used to prepare DNA libraries for DNA sequencing with the ThruPLEX Tag-seq Kit (Takara Bio, USA). The libraries were then pooled and hybridized with pre-designed probes for 95 targeted genes (Integrated DNA Technologies ...
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bioRxiv - Biochemistry 2023Quote: ... and 0.5 µl used as a source of genomic DNA in 20 µl PCR reactions with PrimeStar Hot Start DNA polymerase (Takara Bio, R010A). Amplified DNA was subcloned with StrataClone Blunt PCR cloning kit (Agilent Technologies ...
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bioRxiv - Plant Biology 2023Quote: ... genomic sequences at the length of 700∼2 000 bp were amplified from genomic DNAs with PrimeStar Max DNA polymerase (Takara, cat. R045A). The resulting fragments were cloned into vector backbones derived from pPY22 (mNeonGreen-Hygromycin B ...
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bioRxiv - Cell Biology 2022Quote: ... The pcDNA5 FRT/TO HA FLAG Hu AASS was then digested with BamHI/ApaI and the DNA fragment was ligated into the pcDNA5 FRT/TO vector using a DNA Ligation Kit (#6023; TaKaRa, Kusatsu, Japan), resulting in pcDNA5 FRT/TO Hu AASS ...
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bioRxiv - Cancer Biology 2024Quote: ... Genomic DNA samples from the patients’s paired tumor tissues and WBCs were used to prepare DNA libraries for DNA sequencing with the ThruPLEX Tag-seq Kit (Takara Bio, USA). The libraries were then pooled and hybridized with pre-designed probes for 95 targeted genes (Integrated DNA Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... and 0.5 μl used as a source of genomic DNA in 20 μl PCRs with PrimeStar Hot Start DNA polymerase (Takara Bio, R010A). The amplified DNA was subcloned with StrataClone Blunt PCR cloning kit (Agilent Technologies ...
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bioRxiv - Developmental Biology 2021Quote: PCRs for were undertaken using ExTaq DNA Polymerase (TaKaRa). Primers used are listed below.
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bioRxiv - Biochemistry 2022Quote: ... Miniprepped DNA was transformed into STELLAR Competent Cells (Clontech) and plated on carbenicillin LB agar plates (as per pPNL6’s resistance marker) ...
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bioRxiv - Neuroscience 2021Quote: ... the DNA was incubated in an InFusion (Takara Bio) reaction with BamHI linearised pGEX-6p-3 ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA ligation was done using standard ligation techniques (Takara DNA Ligation kit ver.2.1 ...
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bioRxiv - Molecular Biology 2021Quote: ... using a Random Primer DNA Labeling Kit (TaKaRa, 6045) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... The SMARTer ThruPLEX DNA-Seq kit (Takara, Rockville, MD) was utilized to generate libraries from 10 μl of sheared cDNA template ...
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bioRxiv - Genomics 2021Quote: ... PrimeSTAR GXL DNA Polymerase (Takara Bio Inc., Shiga, Japan) was used.
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bioRxiv - Zoology 2021Quote: ... Genomic DNA was subsequently removed using DNase I (Takara). Meta-transcriptome library preparation of each pool was carried out using the TruSeq™ Stranded Total RNA Sample Preparation Kit from Illumina (San Diego ...
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bioRxiv - Microbiology 2021Quote: ... and genomic DNA was removed using DNase I (TaKaRa). The RNA-seq transcriptome library was prepared following the TruSeqTM RNA sample preparation kit from Illumina (San Diego ...
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bioRxiv - Biochemistry 2020Quote: DNA polymerases PrimeSTAR Max (TaKaRa Bio Inc., Shiga, Japan) and KOD-Plus (Ver.2 ...
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bioRxiv - Plant Biology 2021Quote: ... or PrimeSTAR HS DNA polymerase (R010A, Takara Clonetech Ltd). The fragments were introduced into pENTR-D-TOPO vector by pENTR™ Directional TOPO Cloning Kit (K2400-20 ...
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bioRxiv - Neuroscience 2020Quote: ... and genomic DNA was removed using DNase I (TaKara). Then RNA quality was determined by 2100 Bioanalyser (Agilent ...
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bioRxiv - Plant Biology 2022Quote: ... polymorpha cDNA using PrimeSTAR HS DNA polymerase (Takara Bio) with primers CDS_MpGUN1_At_gib_F and CDS_MpGUN1_At_gib_R ...
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bioRxiv - Microbiology 2022Quote: ... and Ex Taq DNA Polymerase (TaKaRa Bio, Cat.:RR001), to add sequencing indexes to each amplicon ...
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bioRxiv - Microbiology 2022Quote: ... PCR was performed using PrimeSTAR Max DNA Polymerase (Takara) with the cDNA as template and virus-specific primers 5’-CAC TCT TTC CCT ACA CGA CGC TCT TCC GAT CT NNN GGT CAC TAG TTG CCT CAT CCG G-3’ and 5’-GAC TGG AGT TCA GAC GTG TGC TCT TCC GAT CTG GTG CAT CTG ATC TCA TTA TTG-3’ ...
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bioRxiv - Molecular Biology 2022Quote: ... pGBKT DNA-BD and pGADT7-AD vectors (Takara Bio) containing cDNAs encoding regions of MYPN ...
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bioRxiv - Molecular Biology 2020Quote: DNA ligation was performed using standard ligation techniques (Takara DNA Ligation kit ver ...
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bioRxiv - Molecular Biology 2022Quote: ... and DNA Single Index Kit - 12S Set A (Takara) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Total RNA was treated with genomic DNA Eraser (Takara) to remove any remaining genomic DNA ...
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bioRxiv - Microbiology 2021Quote: ... The DNA fragments amplified by high fidelity PCR (Takara) were digested with the appropriate restriction enzymes ...
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bioRxiv - Genomics 2021Quote: ... 1 μL of T4 DNA ligase (Takara Bio Inc.) was added ...
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bioRxiv - Evolutionary Biology 2020Quote: Gflex DNA polymerase (1.25 units/μl) (TaKaRa Bio Inc.), 200nM of each primer ...
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bioRxiv - Cell Biology 2020Quote: ... DNA contamination was eliminated with gDNA Eraser (Takara, RR047A) and reverse transcription of mRNA was conducted using the PrimeScript™ RT reagent Kit (Takara ...
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bioRxiv - Biochemistry 2021Quote: ... and 1.5 U of rTaq DNA polymerase (TaKaRa, Japan). Thirty-five cycles of amplification were performed in a Stratagene Robocycler model 96 thermocycler after initial denaturation of DNA at 95°C for 5 min ...