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Citations for Takara Bio :
401 - 450 of 6112 citations for Cystatin C ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... Total RNA was isolated using Quick-RNA miniprep kit (ZymoResearch, CA, USA) and 1 μg of RNA was used to generate cDNA using SMART MMLV reverse transcriptase (Takara Bio USA, Inc.). Transcript abundance of an ABA biosynthesis gene ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: The cDNA was synthesized using 1 µg of RNA from each treatment using the PrimeScript™ RT reagent Kit with gDNA Eraser (TaKaRa Dalian, China). RT-qPCR was performed on a Bio-Rad CFX96 (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... Approximately 1 µg of total RNA was used for reverse transcription using the PrimeScript™ RT reagent kit with gDNA Eraser (Takara, Beijing, China). After the transcribed cDNAs were 5-fold diluted ...
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bioRxiv - Molecular Biology 2023Quote: ... the first-strand cDNA was synthesized from 1 μg total RNA using PrimeScript™ II 1st Strand cDNA Synthesis Kit (Takara, Beijing, China) following the manufacturer’s instructions.
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bioRxiv - Plant Biology 2023Quote: Genomic DNA was removed from 1 μg purified RNA using gDNA Eraser (PrimeScript RT Reagent Kit, Perfect Real Time) following manufacturer’s instructions (Takara Bio Inc., Kusatsu, Japan). Reverse transcription and cDNA was synthesized using PrimeScript Reverse Transcriptase (TaKaRa Bio) ...
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bioRxiv - Immunology 2021Quote: ... cDNA was synthesized for 90 minutes at 42°C with 170 U SMARTscribe reverse transcriptase (Takara, Clontech) in a total volume of 20μl containing 1.7U/μl RNasin (Promega) ...
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bioRxiv - Immunology 2021Quote: ... cDNA was synthesized for 90 minutes at 42°C with 170 U SMARTscribe reverse transcriptase (Takara, Clontech) in a total volume of 20μl containing 1.7U/μl RNasin (Promega) ...
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bioRxiv - Cell Biology 2019Quote: ... The blots were incubated overnight at 4°C with a mouse anti-GFP antibody (RRID:AB_2323808, 63281, Clontech) at a 1:2,000 dilution in blocking buffer ...
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bioRxiv - Cell Biology 2019Quote: The plasmid pDendra2NatMX was constructed by ligation of the NheI-HpaI Dendra2 fragment from pDendra2-C (Clontech) and the PvuII linearized pAG25 vector (Addgene) ...
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bioRxiv - Cancer Biology 2020Quote: ... Complex formation was induced in these cells by treating with AP21967 (A/C ligand heterodimerizer; Takara Bio). MDA-MB-231-iDimerize-c-Met-β1/luc cells were created by lentiviral transduction of MDA-MB-231-iDimerize-c-Met-β1 cells and subsequent confirmation of bioluminescence ...
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bioRxiv - Synthetic Biology 2019Quote: ... A 1000X stock of rapalog (A/C heterodimerizer AP21967) was obtained commercially (0.5mM, Takara Cat. No. 635056) and stored at −20°C ...
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bioRxiv - Microbiology 2019Quote: ... The NusA tag of GfsB was cleaved with a HRV 3C protease at 4°C (Takara Bio) and removed by Ni-agarose ...
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bioRxiv - Microbiology 2021Quote: ... complementary to the DENV 3’UTR at 65°C for 3 hrs using ExpressHyb hybridization buffer (Clontech). Autoradiographs were quantified using ImageQuant (GE Healthcare).
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bioRxiv - Cell Biology 2022Quote: ... full-length GIP-2 cDNA with a C-terminal V5-His6 tag was inserted into pColdI (TAKARA) and used to inoculate rabbits and rats ...
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Bilateral regulation of EGFR activity and local PI dynamics observed with superresolution microscopybioRxiv - Cell Biology 2024Quote: ... EGFR fused with 6xHis at the C-terminus was cloned into the pTRE-Dual vector (TaKaRa Bio).
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Effects of Sanhuang plaster on expression of MyD88, TRAF6, MIP-1β and IL-23 in rats infected by MRSAbioRxiv - Molecular Biology 2022Quote: ... Real-time fluorescence quantitative PCR kit and reverse transcription kit(TaKaRa Co., Ltd.); MyD88 Anti-body TRAF6 Anti-body MIP-1β Anti-body and IL-23 Anti-body (GeneTexCo. ...
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bioRxiv - Biophysics 2020Quote: ... using a cDNA synthesis kit (PrimeScript 1st strand cDNA Synthesis Kit, Takara Bio), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Adeno-X Maxi Purification Kit and Adeno-X Rapid Titer Kit from Clontech were used for purification and titration ...
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bioRxiv - Cancer Biology 2023Quote: ... Adeno-X Maxi Purification Kit and Adeno-X Rapid Titer Kit from Clontech were used ...
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bioRxiv - Immunology 2021Quote: ... 24-well non-tissue culture plate wells were pre-coated with RetroNectin® (Takara Bio USA, Inc.) according to manufacturer’s instructions and then day 10 cultured Tregs were added at 0.3 x 106 cells/well in 0.3mL of cell culture media ...
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bioRxiv - Molecular Biology 2020Quote: Lysis plates were created by dispensing 0.4 μl lysis buffer (0.5U Recombinant RNase Inhibitor (Takara Bio, 2313B), 0.0625% Triton™ X-100 (Sigma ...
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bioRxiv - Immunology 2020Quote: ... Activated NK cells were transduced with retroviral supernatants on day +4 in human fibronectin-coated plates (Clontech Laboratories ...
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bioRxiv - Cancer Biology 2020Quote: ... Each well of the 96-well plate contained 3µL lysis buffer (10U Recombinant RNase Inhibitor (Takara Bio), 0.2% Triton X-100 (Sigma-Aldrich) ...
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bioRxiv - Systems Biology 2022Quote: ... In brief we sorted labeled T cells into 384 well plates (Armadillo) containing 3μl of Smart-seq3 lysis buffer (0.04μl RNAse inhibitor (40U/μl RRI, TaKaRa), 0.1% Triton X-100 ...
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bioRxiv - Microbiology 2022Quote: ... treatment was used for cDNA library preparation using Make Your Own “Mate & Plate™” Library System (TaKaRa). All the procedures were followed according to the manual provided ...
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bioRxiv - Microbiology 2022Quote: ... Make Your Own “Mate & Plate™” Library System and Yeast Transformation System 2 were purchased from TaKaRa. Synthetic-defined (SD ...
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bioRxiv - Cancer Biology 2022Quote: ... Retroviruses corresponding to different CAR constructs were transduced into activated T cells in retronectin-coated plates (Takara). Cultures were allowed to expand for 10 days in their media supplemented with 300 IU ml-1 IL-2 ...
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bioRxiv - Immunology 2019Quote: ... Retroviral supernatant was added to non-treated retronectin-coated (10 µg/ml) 6-well plates (Takara Bio) and spun for 30 min (1,200 x g ...
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bioRxiv - Plant Biology 2021Quote: ... the cDNA library was prepared using the “mate and plate” library preparation system (Clontech, Mountain View, USA). The prepared library ...
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bioRxiv - Cancer Biology 2019Quote: Lysis plates were created by dispensing 0.4 µl lysis buffer (0.5U Recombinant RNase Inhibitor (Takara Bio, 2313B), 0.0625% TritonTM X-100 (Sigma ...
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bioRxiv - Immunology 2023Quote: ... Transduction was performed on non-tissue culture 24-well plates previously coated with 0.5mL of RetroNectin (Takara) at a concentration of 25 μg/mL in PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... Activated T cells were transduced with the retroviral supernatant using retronectin-coated plates (Takara Bio Inc, T100B). T cells were harvested after three days and expanded in complete medium (45% RPMI-1640 (Corning ...
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bioRxiv - Plant Biology 2019Quote: ... A first-strand cDNA was obtained from 1 μg of total RNA using a PrimeScript® RT reagent kit (Takara Bio, Inc., Otsu, Japan). The sequences of candidate genes were obtained from the local alkaligrass EST database using a BLASTn program ...
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bioRxiv - Molecular Biology 2020Quote: ... The first-strand cDNA was synthesized with 1 μg of total RNA using a PrimeScript™ 1st Strand cDNA Synthesis Kit with gDNA Eraser (TaKaRa Biotechnology, Dalian, China). For sequencing analysis of the PxRdls ...
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bioRxiv - Developmental Biology 2020Quote: ... Lentiviral supernatant titers were determined by Lenti-X p24 Rapid Titer Kit (supplemental Table 1) according to manufacturer’s protocol (Takara Bio USA, Inc. California, U.S.A).
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bioRxiv - Cell Biology 2019Quote: ... The cDNA was synthesized by reverse transcription using 1 μg of total RNA as a template with a PrimeScript™ RT Reagent Kit with gDNA Eraser (Takara, Beijing, China, RR047A). The SYBR® Premix Ex Taq TM II kit (Takara ...
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bioRxiv - Neuroscience 2020Quote: ... and a portion (1 ng) of the RNA were subjected to reverse transcription (RT) with a SMARTer™ PCR cDNA Synthesis Kit (Clontech, Mountain View, CA). Then ...
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bioRxiv - Evolutionary Biology 2022Quote: ... with 0.1 µL of Taq Polymerase 5 U.µL-1 (TaKaRa Ex Taq® Kit MgCl2 Free Buffer) (TaKaRa Ex Taq, TaKaRa Bio, Shiga, Japan), 2.5 µL PCR Buffer 10X ...
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bioRxiv - Cancer Biology 2022Quote: Human c-Jun sequence was amplificated using the cDNA of MCF7-BM02-1 and then transferred to pMXd3-PEF1-IRES-Puro vector using an In-Fusion Advantage PCR Cloning Kit (Clontech Laboratories Inc., CA, USA). The sequence encoding the transactivation domain of c-Jun in pMXs-Jun-IH was kindly provided by Dr ...
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bioRxiv - Neuroscience 2023Quote: ... A total of 1 µg of RNA was reverse transcribed to complementary DNA using the PrimeScript™ RT reagent Kit (Takara Bio, Otsu, Japan), according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... From cortical neurons subjected to OGD we generated 1) library to detect circularRNAs and mRNA and lncRNAs using SMARTer® Stranded Total RNA Sample Prep Kit (Takara Bio USA, Inc.) and 2 ...
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bioRxiv - Systems Biology 2022Quote: ... The SMART-Seq Library Prep Kit and Unique Dual Index Kit (TaKaRa, cat# R400745) were used to generate barcoded template library for sequencing ...
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bioRxiv - Genetics 2021Quote: ... followed by a final extension at 72 °C for 10 min by using Takara Taq polymerase (Clontech, #TAKR001). To remove the DsRed cassette ...
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bioRxiv - Microbiology 2020Quote: ... for 15 min at 37°C in the presence of 80 U of recombinant RNase inhibitor (Takara Bio). Next ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the membrane was incubated overnight at 4°C with an anti-GFP monoclonal primary (JL-8; Clontech 632381) at 1:5,000 dilution ...
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bioRxiv - Genetics 2019Quote: ... following 0.5 mM IPTG induction for 4 h at 25°C with subsequent purification using TALON chromatography (Clontech) as described 34 ...
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bioRxiv - Developmental Biology 2020Quote: ... musculus Dyrk2 cDNA was cloned into pcDNA4/TO-C-3xFLAG using the In-fusion HD cloning system (Clontech). Dyrk2 cDNA clone #6808145 was purchased (ThermoFisher Scientific) ...
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bioRxiv - Developmental Biology 2022Quote: ... The expression of Dunk protein was confirmed by Western blot using c-Myc Monoclonal Antibody (Takara, Cat# 631206). Then ...
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bioRxiv - Genomics 2021Quote: Histone H4 was then cloned into the multiple cloning site of the pDendra2-C vector (Clontech catalog# 632546). The dCas9-3xGFP and C9-sgRNA plasmid for CRISPR imaging were a kind gift of Dr ...
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bioRxiv - Neuroscience 2022Quote: ... pcDNA3.1-DCCR1343H-HA (c.4028G>A, p.R1343H) was derived from pcDNA3.1-DCCWT-HA using In-Fusion (Clontech 639648). Next ...