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Citations for Takara Bio :
4401 - 4450 of 4901 citations for Uridine Triphosphate UTP CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: Complementary DNA (cDNA) was generated from previously extracted total RNA (900 ng) using the SMARTER RACE 5’/3’ kit (Takara Bio USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... One microgram of purified RNA from each sample was used for reverse transcription reaction to generate cDNA with a high-capacity cDNA reverse transcription kit (Takara, Dalian, China) and the resulting cDNA was then used for qPCR on a real-time PCR detection system (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... The qPCR reactions were done on Roche LightCycler® 96 PCR system with the SYBR Premix Ex Taq™ Kit (TAKARA, RR420A). Gene expression levels were normalized to GAPDH and compared to gene expression levels in hESCs ...
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bioRxiv - Plant Biology 2021Quote: ... The PCR products were purified using Dyne PCR Purification Kit (DyneBio, South Korea) and cloned into pPZP211 vectors using Takara T4 DNA Ligase (Takara Bio, Japan). Escherichia coli (DH5α ...
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bioRxiv - Plant Biology 2021Quote: ... 1 ug of total RNA was reverse-transcribed into cDNA using PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) (Takara, Japan). qPCR or qRT-PCR was performed using TB Green® Premix Ex Taq™ II (Takara ...
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bioRxiv - Microbiology 2021Quote: ... The WNV replicon RNAs were isolated from the pellet and reverse-transcribed using Prime Script™ RT reagent Kit with gDNA Eraser (Takara, Japan). Quantitative PCRs were performed using a SYBR PremixEX Taq II (RT)-PCR kit (Takara ...
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bioRxiv - Microbiology 2021Quote: ... The LAMP1 gene was then sub cloned into the pEF1α-mCherry-N1 vector using the In-Fusion® HD Cloning Kit (Clontech, Takara, Japan), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... and subsequently cellular RNA was extracted at 6 hpi using a CellAmp Direct RNA Prep Kit (3732, Takara Bio Inc., Shiga, Japan). The qRT-PCR assays were performed to quantify the amount of SARS-CoV-2 RNA with QuantStudio 3 instrument (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... This was replaced with a golden gate-compatible (BsaI restriction enzyme site-flanked) MCS from pICH86988 using In- Fusion cloning according to kit instructions (Takara Bio, USA) to generate a golden gate cloning-compatible version of the pK18mobsacB vector ...
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bioRxiv - Synthetic Biology 2021Quote: ... The amplified fragments were cloned into a modified pET16b expression vector (Table 2) by using In-Fusion cloning kit (Takara, Shiga, Japan). The sequence of the inserts of the resulting plasmids was verified by Sanger sequencing ...
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bioRxiv - Microbiology 2020Quote: ... RNA was reverse-transcribed into cDNA using random hexamer primers and the PrimeScript RT Reagent Kit with gDNA Eraser (Perfect Real Time; Takara, Dalian, China). For the target genes ...
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bioRxiv - Microbiology 2020Quote: ... RNAs extracted from 100 µl of virus-containing supernatants or PBMCs were amplified by using a PrimeScript® RT reagent Kit (Perfect Real Time) (Takara Bio) and 5’RACE was performed by using a 5’RACE System for Rapid Amplification of cDNA Ends ...
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bioRxiv - Microbiology 2020Quote: ... Ad5 stocks were tittered by detection of the Ad hexon protein using Adeno-X™ Rapid Titer Kit (Cat. No. 632250, Takara, Clontech). The Ad5 CPE was dose-dependent ...
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bioRxiv - Cancer Biology 2021Quote: ... and the total extracted RNA was reverse-transcribed into cDNA for polymerase chain reaction amplification using the real-time polymerase chain reaction SYBR Green kit (TaKaRa, Guangzhou, China). The reverse transcription steps were as follows ...
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The pan-cancer lncRNA PLANE regulates an alternative splicing program to promote cancer pathogenesisbioRxiv - Cancer Biology 2020Quote: ... cDNA was synthesized from 1 μg of total RNA using the PrimScriptTM RT reagent Kit with gDNA Eraser (TaKaRa, #RR047A; Dalian, China). Of the resultant cDNA ...
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bioRxiv - Cell Biology 2021Quote: 5’ and 3’ RACE reactions were performed to isolate full-length lncEry from the total RNA of MEP cells using the 5’- and 3’-Full RACE Kits (TaKaRa, Dalian, China) in accordance with the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... and the RNA library was prepared with the SMARTer Stranded Total RNA Sample Prep Kit - Low Input Mammalian (Takara catalog number 634861) and sequenced as described previously (29).
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bioRxiv - Cancer Biology 2022Quote: ... 0.25 ng to 10 ng of total RNA was used for cDNA library preparation using a kit suitable for RNA isolation at pico-molar concentrations (MARTer Stranded Total RNA-Seq Kit v2-Pico Input Mammalian) following manufacturer recommended protocol (Clontech/Takara Bio #635005). Sequencing was performed by the UM DNA Sequencing Core ...
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bioRxiv - Cell Biology 2022Quote: ... SEPT2 constructs in pTRIP TRE Bi plasmids and all interface mutants in pTRIP TRE Bi plasmids were cloned using seamless cloning (In-Fusion HD Cloning Plus Kit from Takara Bio, 638910). All pcDNAs and all wild-type SEPT7 and SEPT9-containing constructs in pTRIP TRE Bi plasmids were generated with classical cloning ...
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bioRxiv - Plant Biology 2022Quote: The coding region of each P450 gene was cloned into pCAMBIA1390 vector with the In-Fusion DH Cloning Kit (TaKaRa, Kusatsu, Japan) or SLiCE reaction (Motohashi ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA (500 ng) from the worms was reverse transcribed into cDNA by using a PrimeScript RT Reagent Kit (TaKaRa, Dalian, China) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: Between 1-3 ng of the nuclear RNA was used in the SMART-seq v4 Ultra Low Input RNA Kit for Sequencing (Takara, cat # 634888) according to the manufacturer’s protocols ...
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bioRxiv - Microbiology 2022Quote: ... cDNA was prepared from 2 µg of each RNA sample using the PrimeScript II 1st strand cDNA synthesis kit (TaKaRa, Shiga, Japan) with 40 units of RNasin Plus RNase inhibitor (Promega ...
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bioRxiv - Cell Biology 2022Quote: ... and a separate master mix was prepared for each primer set using the TB Green® Premix Ex Taq II (TLi RNaseH Plus) reagent kit (RR820A, Takara Bio). The final master mix contained primers at a concentration of 0.4 µM and a 1X concentration of TB Green® Premix Ex Taq II (TLi RNaseH Plus) ...
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bioRxiv - Bioengineering 2022Quote: 500 ng of total RNA extracted from cell culture were subjected to reverse transcription, using ISOGEN II (NIPPON GENE, Tokyo, Japan) and PrimeScript RT reagent Kit (TaKaRa, Kusatsu, Japan). The resulting cDNA was subjected to real-time PCR analysis using glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Neuroscience 2020Quote: For SVZ tissue libraries were prepared using SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing (Takara Bio USA) performing 10 cycles of amplification ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... served as template in a 20 μl qRT-PCR using the TB Green Premix Ex Taq II (Tli RNaseH Plus) kit (Takara Bio Inc.) and the Roche LightCycler 96 system (Roche ...
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bioRxiv - Developmental Biology 2021Quote: SMART-Seq v4 Ultra Low Input Kit for Sequencing was used for full-length cDNA synthesis and amplification (Clontech, Mountain View, CA), and Illumina Nextera XT library was used for sequencing library preparation ...
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bioRxiv - Plant Biology 2021Quote: ... A 1 μg aliquot of RNA was used for the synthesis of the cDNA first strand using a PrimeScriptTMRT reagent Kit containing gDNA eraser (TaKaRa, Shiga, Japan). The cDNA was used as the template and primers in TableS1 were used to PCR amplify the sequence ...
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bioRxiv - Molecular Biology 2020Quote: ... Pooled RNA from two previously identified positive NP swab samples was used for first strand cDNA synthesis (Takara primescript kit cat # 6110A). 20 μl of the cDNA reaction was diluted ten-fold and used for primary PCR ...
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bioRxiv - Molecular Biology 2021Quote: ... The chip was then loaded again on Fluidigm C1 and cDNA was synthesised and pre- amplified in the chip using Clontech SMARTer kit (Takara Clontech, Cat# 634833). In batch B ...
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Targeted attenuation of elevated histone marks at SNCA alleviates α-synuclein in Parkinson’s diseasebioRxiv - Neuroscience 2020Quote: ... The RNA was eluted according to the protocol in 10 uL nuclease free water and equal amounts of RNAs from different samples were immediately subjected to amplification-based cDNA synthesis using the SMARTer PCR cDNA Synthesis Kit (Takara, 634925, 639207). We have standardized the amplification cycle to 15 for 2-10 ng of initial RNA template used for the reaction ...
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bioRxiv - Microbiology 2020Quote: ... Cell supernatants were harvest 24 hours post-infection and RNA was extracted by a viral RNA extraction kit (Takara, Cat no. 9766). The number of viral copies was detected by real-time quantitative reverse transcription PCR (qRT-PCR ...
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bioRxiv - Neuroscience 2021Quote: ... After the immortalization procedure the absence of virus was verified using the Lenti-X p24 Rapid Titer Kit (Takara Bio USA, #632200).
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bioRxiv - Microbiology 2021Quote: Equal amounts of RNA were converted into cDNA using Prime Script™ RT Reagent Kit with gDNA Eraser (Perfect Real Time) (RR047A, Takara-Bio) and then diluted with 80μl nuclease-free water ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were later processed for single cell RNA-seq using SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara Cat# 634894) as previously described41,45.
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bioRxiv - Neuroscience 2021Quote: ... were produced and purified in the house using an established protocol (Challis et al., 2019) and commercial purification and titration kits (Takara Bio, USA).
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bioRxiv - Plant Biology 2020Quote: ... and one microgram of the DNase-treated RNA was used for cDNA synthesis using PrimeScript RT reagent kit (Takara Bio, CA, USA). The expression analysis was performed using TB green Premix Ex Taq II (Takara Bio ...
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bioRxiv - Microbiology 2022Quote: 1 μg of RNA was reverse transcribed into cDNA using Prime Script™ RT Reagent Kit with gDNA Eraser (Perfect Real Time) (RR047A, Takara-Bio) and then diluted 5-fold with nuclease-free water ...
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bioRxiv - Pathology 2022Quote: ... and expected amplicons of NS1 and VP1 genes were purified using Nucleospin® Gel and PCR clean-up kit (Takara Bio, Japan). Purified PCR amplicons were individually subjected to Barcode-Tagged sequencing (Celemics ...
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bioRxiv - Evolutionary Biology 2022Quote: ... library was prepared according to the Isoform Sequencing protocol (Iso-Seq) using the Clontech SMARTer® PCR cDNA Synthesis Kit (Clontech Laboratories (now Takara Laboratories), 634926 ...
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bioRxiv - Genetics 2022Quote: SMART-Seq v4 Ultra Low Input Kit for Sequencing was used for full-length cDNA synthesis and amplification (Clontech, Mountain View, CA), and Illumina Nextera XT library was used for sequencing library preparation ...
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bioRxiv - Immunology 2022Quote: ... cDNA was synthesized to a total of 500ng RNA in a 20 μL system using PrimeScript™ RT Reagent kit (TAKARA, #RR036A). q-PCR were performed on anlytik jena (qTOWER 2.0 ...
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bioRxiv - Immunology 2022Quote: ... SMART-Seq v4 Ultra Low Input Kit for Sequencing was used for full-length cDNA synthesis and amplification (Clontech, Mountain View, CA), and Illumina Nextera XT library was used for sequencing library preparation ...
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bioRxiv - Neuroscience 2022Quote: ... double-stranded cDNA whole transcriptome library was synthesized from 1µg of total RNA with Takara Bio’s SMARTer Stranded Total RNA Sample Prep Kit – HI Mammalian (Takara Bio, Cat. No. 634876) and SMARTer RNA Unique Dual Index Kit (Takara Bio ...
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bioRxiv - Molecular Biology 2022Quote: ... qRT-PCR was perfor med using a qPCRsoft (Analytik Jena AG, Germany) with a SYBR®Green PC R Master Mix Kit (Takara Bio), according to the manufacturer’s instructions ...
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bioRxiv - Zoology 2019Quote: ... the cDNA ends of both genes were obtained via 5’- and 3’-RACE using the SMARTer™ RACE cDNA amplification kit (Clontech, USA). Resulting PCR products were directly sequenced and full-length cDNAs of the putative mudskipper desaturase and elongase were constructed by aligning overlapped regions of the cDNA fragments.
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bioRxiv - Evolutionary Biology 2019Quote: ... This vector was generated by splicing PCR products of the MpALSm gene with the 35S promoter together with the MpEF1α promoter into a XhoI/PstI-digeststed pCAMBIA backbone using the Clontech In-Fusion kit (Clontech Cat. #: 638909) and the primers indicated in Table 1 ...
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bioRxiv - Molecular Biology 2019Quote: The transcription initiation and termination sites of OGRU were detected by 5’ and 3’ RACE using a SMARTer® RACE 5’/3’ Kit (Clontech, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... Full-length cDNA fragments were transcribed and amplified from poly-A RNA in each single cell using the SMARTer Ultra Low RNA kit (Takara Bio, 634832). Tagmentation of cDNA was performed and sequencing libraries were prepared using the Nextera XT DNA sample preparation kit (Illumina ...