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Citations for Takara Bio :
4201 - 4250 of 5458 citations for Rat Insulin Like Growth Factor Binding Protein 4 IGFBP4 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... 5’-RACE cDNA was obtained from bulk-sorted splenic B cells of each mouse with SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (TaKaRa). The immunoglobulin PCRs were set up with Platinum Taq High-Fidelity DNA Polymerase (Life Technologies ...
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bioRxiv - Pathology 2020Quote: ... and then 8μL of each was reverse transcribed into cDNA by using the commercial company's reverse transcription kit (TAKARA No. 6110A). The full-length spike gene (3882 nt ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: 5’ and 3’ cDNA ends were subsequently amplified by RACE PCR using the SMARTer RACE cDNA Amplification Kit and Advantage HF2 DNA polymerase (Clontech) with gene-specific primers derived from the initial RT-PCR product (Supplemental Information ...
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bioRxiv - Genetics 2021Quote: ... This mutated fragment was replaced by the original homologous sequence of the pJet-ebony_mut-mCherry vector by fusing the mutated fragment and the PCR product amplified from the pJet-ebony-mCherry vector using the In-Fusion HD Cloning Kit (TaKaRa) with the following primers ...
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bioRxiv - Genetics 2021Quote: ... Reverse transcription was then performed using the oligo (dT) primer and Avian myeloblastosis virus reverse transcriptase contained in the TaKaRa RNA PCR kit (TaKaRa). We conducted RT-PCR using KOD One (TOYOBO ...
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bioRxiv - Genetics 2021Quote: ... fust-1 genomic fragment containing sequences from 2181bp upstream ATG to just before stop codon was cloned into the Sma I site of pHK-mRFP vector in frame with mRFP by In-Fusion HD Cloning Kit (Takara). This plasmid was further used to generate backbone structure containing fust-1 promoter and mRFP ...
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bioRxiv - Cell Biology 2019Quote: ... First-strand cDNA was synthesized from l μg of RNA using the PrimeScript RT Reagent Kit with gDNA Eraser (catalogue No. RR047A, TaKaRa). qPCR was performed in triplicate in 20-μl reactions containing SYBR Premix Ex Taq II (catalogue No ...
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bioRxiv - Microbiology 2021Quote: ... extracted RNA was reverse-transcribed into cDNA using the PrimeScript™ RT reagent Kit with gDNA Eraser (TaKaRa, Dalian, China). The gene quantitative PCR was performed using TB green Premix EX Taq (Takara ...
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bioRxiv - Cell Biology 2020Quote: ... we first inserted the mAID sequence after 3xFlag in pCRISPaint-3xFlag-puro (Schmid-Burgk et al., 2016) using the In-Fusion HD Cloning kit (Takara) to generate pCRISPaint-3xFlag-mAID-puro ...
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bioRxiv - Immunology 2019Quote: ... cDNA libraries for alveolar macrophages were constructed and amplified using the SMARTer Stranded Total RNA-Seq Kit v2 – Pico Input Mammalian (Clontech) per the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... was added to mitigate DNA contamination prior to first-strand cDNA synthesis with PrimeScript™ RT reagent kit (Takara Bio) from approximately 1 g total RNA ...
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bioRxiv - Neuroscience 2020Quote: ... Ten nanograms of total RNA were amplified and converted into cDNA using a SMART-Seq v4 Ultra Low Input RNA kit (Clontech). Next ...
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bioRxiv - Molecular Biology 2020Quote: ... pnYK vector was created from pnYC vector (58) by homologous recombination between the chloramphenicol and kanamycin genes resistance gene using the In-Fusion kit (Clontech) following the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2021Quote: ... Two-point five microliters of the polyadenylated sample was used for cDNA synthesis by the PrimeScript RT reagent kit with gDNA eraser (TaKaRa), with the y625 primer in Table S1 ...
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bioRxiv - Molecular Biology 2021Quote: ... Homology arms complementary to the pUC19 cloning vector were then added to the PCR product with another PCR in order to clone the RACE products with the In-Fusion HD Cloning kit (Takara). Individual clones were selected and Sanger sequenced.
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bioRxiv - Microbiology 2020Quote: ... Approximately 1,000 ng of RNA was reverse transcribed using the PrimeScript RT Reagent Kit with gDNA Eraser (TaKaRa, Dalian, China) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... For analysis of the NAD metabolome HEK293T cells were transfected with 1µg of pEGFP-C1 Empty Vector or pEGFP-C1-CMV-PARP10 using CalPhos Mammalian Transfection Kit (Takara Bio). 6 hours later the cells were treated with chemical treatments in DMEM + 10% FBS at 37°C 5% CO2 for 18h.
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bioRxiv - Microbiology 2020Quote: ... according to the manufacturer’s instructions and detected by RT-qPCR assays with a One-Step PrimeScript RT-PCR kit (Takara, Japan) using SARS-CoV-2-specific primers on an Applied Biosystems 7500 Real-time PCR System.
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bioRxiv - Synthetic Biology 2020Quote: ... The amplified fragments were gel-purified and fused using an In-Fusion® HD Cloning Kit (Takara Bio, Shiga, Japan). Full sequences of the plasmids used in this study are provided in Supplementary Information I ...
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bioRxiv - Bioengineering 2020Quote: ... and recovery of ribosome footprints were performed as described previously.[55] Footprints were dephosphorylated and cDNA libraries were prepared using the SMARTer smRNA-Seq Kit (Clontech), with an additional rRNA depletion step prior to PCR amplification ...
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bioRxiv - Pathology 2020Quote: ... Single stranded cDNA was synthesized with the oligo(dT) primer using PrimeScript™ RT reagent Kit with gDNA Eraser (Takara), the obtained cDNAs were analyzed by real-time PCR ...
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bioRxiv - Cell Biology 2020Quote: ... according to the manufacturer’s protocol.Forty ng cDNA was used as templates for RT-PCR using SYBR® Premix Ex Taq kit (TaKaRa,#RR820A) using LightCyclerW 480 (Roche ...
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bioRxiv - Cell Biology 2020Quote: Four hundred ng total RNA was employed for cDNA synthesis using ProtoScript II First Strand cDNA Synthesis kit(TaKaRa,#RR036A) according to the manufacturer’s protocol.Forty ng cDNA was used as templates for RT-PCR using SYBR® Premix Ex Taq kit (TaKaRa,#RR820A ...
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bioRxiv - Molecular Biology 2020Quote: ... The amplified DNAs were subcloned into the pCK-FLAG vector (CMV promoter-driven vector) at the BamHI and XhoI sites using In-Fusion HD Cloning Kit (Clontech). For dsRBD-deleted DICER ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The purification of the digested DNA and the ligation to the GenomeWalker adaptors were carried out according to the manufacturer’s instructions of the GenomeWalker Universal Kit (Clontech Laboratories). PCR reactions contained in a total volume of 50 µl 1x Long PCR buffer with MgCl2 ...
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bioRxiv - Plant Biology 2019Quote: ... and oligodT primer were used for cDNA synthesis with PrimeScript RT Reagent Kit (Perfect Real Time) following the manufacturer’s instructions (Takara Bio). An amount of 5 μl of 25X dilute cDNA was used as a template for ddPCR reaction (total 20 μl ...
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bioRxiv - Developmental Biology 2019Quote: ... 10 ng total RNA was reverse transcribed and full-length cDNA was specifically amplified by 8 PCR cycles using SMART-Seq v4 Ultra Low Input RNA Kit (Clontech) (31) ...
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bioRxiv - Plant Biology 2019Quote: Rapid amplification of 5’ cDNA ends (5’RACE) of CREF3 transcripts was performed using SMARTer RACE 5’/3’ Kit (Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... Total RNA was used to synthesize cDNA with a PrimeScript II 1st Strand cDNA Synthesis Kit (D6210A; TaKaRa, Dalian, China) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... The cDNA was generated through a 3’-RACE-Ready cDNA synthesis reaction using the SMARTer RACE cDNA amplification kit (Clontech), following the user manual ...
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bioRxiv - Developmental Biology 2020Quote: ... and then further into the ClaI site of the avian replication-competent retrovirus vector RCASBP(B) (Li, Monckton, & Godbout, 2014) with the In-Fusion HD cloning kit (Clontech). The mutant construct that converts glutamine (Q ...
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bioRxiv - Molecular Biology 2019Quote: ... Total RNA was reverse transcribed to complementary DNA (cDNA) using a Prime Script™ RT Master Mix Kit (TaKaRa, China) and the following procedure ...
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bioRxiv - Cell Biology 2019Quote: ... PCR fragments containing M1 Spastin amino acids 197-328 was inserted into the C-terminus of FP-M11-92 linearized by BamHI enzyme using In-Fusion cloning kit (Takara). DsRed-M11-92-197-328 was constructed by replacing mApple in mApple-M11-92-197-328 with DsRed2 using NheI and EcoRI restriction sites ...
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bioRxiv - Microbiology 2019Quote: ... Reverse transcription was performed on 2 μg of total RNA using the Primescript Reverse Transcription Kit with a mixture of oligo-dT primers and random hexamers (Takara) after TURBO™ DNase (ThermoFisher ...
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bioRxiv - Molecular Biology 2019Quote: ... and then each RNA sample (1 μg) was reverse transcribed using the Prime Script first-strand cDNA synthesis kit (catalog no. 6110A; TaKaRa). The internal control for qRT-PCR experiments was the 18S rRNA gene of BPH ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Forward (control) and reverse (target) RNA probes were synthesized from plasmids amplified via PCR (Advantage HD Polymerase Kit, Takara Bio) using T7 or SP6 RNA Polymerases (ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2019Quote: ... Total RNA was reverse transcribed into cDNA using SYBR® Premix DimerEraser™ (perfect Real Time) Kit (Takara, Shiga, Japan). Six pairs of specific primers were designed to amplify the genes selected from multiple comparisons (Table S1) ...
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bioRxiv - Microbiology 2019Quote: ... A 500 ng of the RNA was used for the 5’RACE reaction with SMARTer RACE 5’/3’ kit (Clontech), according to manufacture’s instruction ...
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bioRxiv - Genetics 2020Quote: ... Microglial RNA was isolated using the TRIzol method and cDNA libraries were generated using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing Components (Takara) according to the manufacturer’s protocol.The libraries were sequenced as 150 bp paired-end reads with an average read depth of 42 million (range 16-98M ...
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bioRxiv - Genetics 2020Quote: ... The guide for Cas9 was obtained by direct hybridization of the oligos and inserted in the same plasmid by Gibson assembly (In-Fusion HD cloning kit; Clontech) using the BtgZI sites.
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bioRxiv - Microbiology 2020Quote: ... Cellular RNA was isolated at 6 hours post-infection (hpi) using a CellAmp Direct RNA Prep Kit (3732; Takara, Japan) and culture medium collected at 24 hpi or 27 hpi was diluted 10-fold in water ...
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bioRxiv - Genetics 2021Quote: ... Samples of 500 ng of RNA were reverse transcribed using PrimeScript™ RT Reagent Kit (Perfect Real Time) (TAKARA, RR037A). qPCR was performed using TB Green Premix Ex Taq reagent (TAKARA ...
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bioRxiv - Cell Biology 2019Quote: ... RNA with an OD value (A260/A280) of 1.8–2.0 was reversed transcribed to cDNA by using a PrimeScriptTM RT-PCR kit (Takara, Kusatsu, Japan). cDNA was amplified by Takara Taq™ (DR001AM ...
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bioRxiv - Cell Biology 2021Quote: ... Viral RNA was quantified using a One Step TB Green PrimeScript PLUS RT-PCR Kit (Perfect Real Time) (Takara Bio) on a StepOnePlus real-time PCR system (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... All constructs were generated with two-insert seamless cloning (In-Fusion HD Cloning Plus Kit from Takara Bio, Cat# 638910) using NheI/BamHI linearized plasmid backbones (Addgene plasmid #54759 ...
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bioRxiv - Microbiology 2020Quote: ... 5′-RACE cDNA was obtained from bulk-sorted B cells of each animal with SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (TaKaRa). The Ig PCRs were set up with Platinum Taq High-Fidelity DNA Polymerase (Thermo Fisher ...
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bioRxiv - Neuroscience 2021Quote: ... AP-MDGA1 and AP-MDGA2 were generated by replacing the V5 tag of the V5-MDGA1 and V5-MDGA2 constructs respectively by the 14 amino acids AP tag (5’GGCCTGAACGAtATCTTCGAGGCCCAG AAGATCGAGTGGCACGAG3’) using the HD-In-Fusion kit (Takara). The linker 5’GGAGGATCAGGAGGATCA3’ was added after the AP tag ...
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bioRxiv - Physiology 2021Quote: ... as well as the left and right homology arms were assembled and cloned into SmaI-digested pBluescriptII SK(-) in a single enzymatic reaction using the In-Fusion Cloning Kit (TAKARA). gRNA vectors were constructed in pDCC690 ...
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bioRxiv - Plant Biology 2021Quote: Double-stranded RNA (dsRNA) of GFP and CHS were synthesized using the in vitro Transcription T7 Kit (Takara, Ohtsu, Japan). Briefly ...
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bioRxiv - Genetics 2021Quote: ... Quantitative PCR was performed using KAPA SYBR Fast qPCR Kits (Nippon Genetics, Japan) on Dice Real Time System Single Thermal Cycler (Takara) or CFX96 Real- Time System (BioRad ...