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Citations for Takara Bio :
4101 - 4150 of 4901 citations for Uridine Triphosphate UTP CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: cDNA was produced in triplicates from three μg of RNA by prime script 1st strand cDNA synthesis kit (Takara-cat # 6110A). Gene expression was scrutinized in reverse transcription-quantitative polymerase chain reaction (RT-qPCR ...
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bioRxiv - Cell Biology 2020Quote: ... Full-length Nrg cDNA obtained from DGRC (clone GH03573) was inserted into the linearized backbone (EcoRI, SfiI) using an In-Fusion HD Cloning Kit (Takara Bio). Site-specific attp40 insertion into the fly genome was performed by Bestgene ...
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bioRxiv - Developmental Biology 2020Quote: ... or into a 200 μl tube containing 5 μl of lysis buffer and 5% RNase inhibitor (for scRNA-seq) (lysis buffer and RNase inhibitor from SMART-Seq v4 Ultra Low Input RNA Kit, Takara, # 634892). Once the dissociation process began ...
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bioRxiv - Developmental Biology 2021Quote: ... and limited cDNA amplification was performed using the SMARTer Ultra Low Input RNA Kit for Sequencing – v4 (Takara Bio USA, Inc.). The resulting full-length cDNA was fragmented and tagged ...
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bioRxiv - Synthetic Biology 2021Quote: ... Aliquots of 1 μg of the treated RNA were used for cDNA synthesis with oligo dT using PrimeScript™ RT-PCR kit (Takara). cDNAs (0.4 μl ...
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bioRxiv - Molecular Biology 2020Quote: ... Illumina data from human HEK293T cells were processed with the SMARTer® smRNA-Seq Kit for Illumina (Takara, Cat. Nos. 635029) following guidelines ...
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bioRxiv - Molecular Biology 2020Quote: ... A full-length cDNA library was constructed for each sample using the TeloPrime Full-Length cDNA Amplification Kit V1 (Lexogen) and amplified using PrimeSTAR GXL DNA Polymerase (Takara Bio) with 22 PCR cycles of 98 °C denaturation for 10 seconds ...
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bioRxiv - Neuroscience 2021Quote: ... RNA samples were reverse transcribed into cDNA and amplified using Smartseq v4 Ultra Low Input RNA Kit for Sequencing (Takara #634889), per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... RNA quality was assessed using an Agilent Bioanalyzer and ten nanograms of total RNA used as input for library preparation using the SMARTer Stranded Total RNA-Seq V2 Pico Input Mammalian kit (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... The full-length cDNA of the NIC1-locus ERF genes were amplified using PrimeScript First-Strand cDNA Synthesis Super Mix Kit (Takara, Japan) from the first-strand cDNA with gene-specific primers with suitable restriction enzyme sites (Additional file 3 ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was reverse-transcribed from 800 ng of total RNA using the PrimeScript™ RT reagent Kit with gDNA Eraser (Takara). Gene-specific primers with appropriate amplification efficiency (0.95–1.05 ...
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bioRxiv - Plant Biology 2021Quote: ... 1 μg of the total RNA from the leaves was reverse-transcribed for 30 min at 42°C in a 20-μl reaction volume using a High Fidelity PrimeScriptTM RT-PCR kit (Takara, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2021Quote: ... which contains a six-amino acid His-tag for the creation of a N-terminal fusion using the In-Fusion HD cloning kit (Clontech, USA).
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bioRxiv - Developmental Biology 2019Quote: ... and subjected to direct cDNA synthesis using the SMART-Seq v4 ultra Low Input RNA Kit for Sequencing (Clontech Laboratories #634888). Approximately 250pg of cDNA was used for preparation of dual-indexed libraries using the Nextera XT DNA Library Preparation Kit (Illumina # FC-131-1002 ...
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bioRxiv - Microbiology 2021Quote: ... leuC and leuD were measured by qRT-PCR using a SYBR Premix Ex Taq (perfect real time) kit (TaKaRa, Dalian, China). The recA gene was used as an internal control for B ...
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bioRxiv - Synthetic Biology 2020Quote: ... Single-transgene pSurvivin-driven PPs were designed and made in-house using In-Fusion HD Cloning Kits (Takara Bio, CA, USA). First ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... One microgram of total cellular RNA was converted to single-stranded cDNA using the PrimeScript 1st Strand cDNA Synthesis Kit (Takara Bio). The cDNA (1 μl ...
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bioRxiv - Microbiology 2020Quote: ... The 5’ RACE reaction was performed with an ac13-specific primer (ac13-GSP1) using a SMARTer® RACE 5’/3’ Kit (TaKaRa) according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The promoter fragment was ligated into the ApaI-linearized pRS41H-lacZ vector using an In-Fusion kit (Takara Bio USA Inc.). The sequence-checked plasmid was transformed into yeasts using the lithium chloride method (Gietz and Woods 2002).
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bioRxiv - Genomics 2021Quote: ... Sequencing libraries of the two MNase treated samples and the input gDNAs were prepared with the PrepX™ DNA Library Kit (Takara). The libraries were used as a template to generate 50 bp paired end (PE ...
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bioRxiv - Plant Biology 2021Quote: ... The Y2H assay was performed following the manual provided in the kit “Matchmaker Gold Yeast Two-Hybrid” (Clontech, Mountain View, USA). The list of VirE2 interacting clones has been given in Fig.S1.
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bioRxiv - Immunology 2020Quote: ... and cDNA libraries prepared and sequenced according to the Illumina TruSeq RNA Sample Preparation Guide for SMARTer Universal Low Input RNA Kit (Clontech, USA) and a HiSeq 2000 system (Illumina ...
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bioRxiv - Immunology 2021Quote: ... First strand cDNA synthesis was performed with 4 μg of total RNA per reaction using PrimeScript™ II 1st Strand cDNA Synthesis Kit and oligo-dT primer (TAKARA) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... was used to clone SARS-CoV-2 S gene into the SFG backbone using the In-Fusion Cloning kit (Takara Bio). pSFG-SARS-CoV-2 S D614G was cloned from pSFG-SARS-CoV-2 S plasmid using the Q5 Site-Directed Mutagenesis Kit (New England BioLabs) ...
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bioRxiv - Systems Biology 2020Quote: ... cDNA libraries were synthesized with 4 ng of total RNA input to the Clontech SMARTer smRNA-seq kit (Takara Bio 635034) using the manufacturer’s suggested protocol ...
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bioRxiv - Genetics 2019Quote: ... The total amount of DNA-free RNA obtained from each tissue was reverse transcribed using the PrimeScript RT reagent Kit (TaKaRa, Japan) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... N-terminal deletion constructs for REH2 (ΔN and ΔNds) and H2F1 (ΔN) were prepared using the In-Fusion® HD Cloning Kit (639648; Clontech). Two PCR amplicons were prepared in each case using CloneAmp™ HiFi PCR Premix (638500 ...
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bioRxiv - Genetics 2021Quote: Isolated RNA from OSF and control tissues was immediately subjected to realtime RT PCR (using Takara PrimescriptTM one step realtime RT PCR kit) (Takara, Japan) analysis for detection of changes in expression of NQO1 mRNA according to the manufacturers protocol ...
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bioRxiv - Neuroscience 2020Quote: ... Each cell was then aspirated a final time and deposited into an individual 0.5mL tube containing 9.5ul of nuclease-free water and 1ul of 10x Reaction Buffer (Smart-Seq V4 Ultra Low Input RNA kit, Takara Bio) and allowed to incubate at room temperature for 5 minutes before being stored at −80deg until cDNA synthesis ...
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bioRxiv - Microbiology 2021Quote: ... Total RNA was reverse transcribed using PrimeScriptTM RT reagent Kit with gDNA Eraser (Perfect Real Time) according to the manufacturer’s protocol (Takara, Kusatsu, Japan). DNA and cDNA samples were subjected to 16S rRNA gene amplicon sequencing ...
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bioRxiv - Plant Biology 2021Quote: ... was used for the first strand cDNA synthesis following the manufacturer’s instructions (SMARTer RACE cDNA Amplification Kit (Clontech, Cat. No.634923)) ...
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bioRxiv - Plant Biology 2021Quote: ... First-strand cDNA was synthesised from 1 μg of total RNA using PrimeScript 1st-Strand cDNA Synthesis Kit (Takara Bio, Japan) as per manufacturer’s protocol ...
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bioRxiv - Physiology 2020Quote: ... 1μg RNA was reverse transcribed into single-stranded cDNA using the SuperScript First-strand Synthesis Kit (Takara Bio Inc., Tokyo, Japan). Gene-specific primers were designed by Beacon Designer 7.9 software (Premier Biosoft International ...
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Altered TGFB1 regulated pathways promote accelerated tendon healing in the superhealer MRL/MpJ mousebioRxiv - Cell Biology 2021Quote: Samples of 1ng of total RNA were pre-amplified with the SMARTer Ultra Low Input kit v4 (Clontech, Mountain View, CA) per manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... The cloned fragment was inserted into the plasmid pPICZαA containing the AOX1 promoter by replacing the fragment between the EcoR I site and Xba I site using an In-Fusion HD cloning kit (Clontech, Japan). To express recombinant proteins with a fluorescent tag ...
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bioRxiv - Microbiology 2020Quote: ... The PCR products were purified and fused into a ClaI-digested plasmid with an In-Fusion HD cloning kit (TAKARA, Japan). Mutants for overexpressing HFB2 (TgOPB38530 ...
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bioRxiv - Neuroscience 2021Quote: ... 1 μg total RNA was used for cDNA synthesis using RNA to cDNA EcoDry™ Premix (double-primed) kits (Clontech, UK). qRT-PCR reactions were performed in triplicate using a Corbett Rotorgene 6000 Real-Time PCR machine with Sensimix SYBR No-Rox (Bioline ...
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bioRxiv - Genomics 2021Quote: ... The IVT RNA is transferred into cDNA with random primers with SMART MMLV kit by following the manufactory protocol (TaKaRa, 639524). 100ng RNA was used for each library preparation ...
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bioRxiv - Microbiology 2020Quote: ... was amplified using primers C.9 and C.10 and cloned into pOPINF using the In-Fusion HD EcoDry cloning kit (Takara Bio).
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bioRxiv - Physiology 2021Quote: ... First-strand cDNA was synthesized from total RNA using a PrimeScript RT Reagent Kit with gDNA Eraser (Takara Bio, Shiga, Japan).
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bioRxiv - Biochemistry 2021Quote: ... Hsf1 CS mutant (C35S/C103S/C153S/C373S/C378S) was then constructed through site-directed mutagenesis using PrimeSTAR Mutagenesis Basal Kit (Takara Bio). All the protein samples were expressed in BL21(DE3 ...
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bioRxiv - Cell Biology 2021Quote: ... where mApple cDNA was inserted between Nhel/EcoRI sites and the GLI1 gene or its variants inserted into EcoRI downstream of mApple using the InFusion cloning kit (Takara Bio).
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bioRxiv - Microbiology 2021Quote: ... The copy number of viral RNA was standardized with a SARS-CoV-2 direct detection RT-qPCR kit (Takara, Cat# RC300A). The fluorescent signal was acquired using a QuantStudio 3 Real-Time PCR system (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... was used as the template for real-time RT-PCR performed according to the manufacturer’s protocol using the One Step TB Green PrimeScript PLUS RT-PCR kit (Takara, Cat# RR096A) and the following primers ...
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Stem cell-derived macrophages as a new platform for studying host-pathogen interactions in livestockbioRxiv - Immunology 2021Quote: ... Filtered lentivirus was either stored in aliquots at −80°C or further purified and concentrated using the Lenti-X Maxi Purification kit (Takara #631234) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... reverse transcription and cDNA amplification were performed on the Fluidigm C1 machine according to the manufacturer’s mRNA-seq protocol using the SMARTer Ultra Low RNA Kit for the Fluidigm C1 System (Takara Bio #634833). cDNA amplicons (∼3 µL ...
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bioRxiv - Developmental Biology 2021Quote: ... 350 ng RNA samples in 6 µL nuclease-free water) were generated using the Clontech SMARTer smRNA-Seq kit using 8 PCR cycles (Takara Bio). 30 µL of the PCR reaction was purified with AMPure XP beads (Beckman Coulter A63880 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... qRT-PCR was performed by using One Step SYBR® PrimeScript® PLUS RT-RNA PCR Kit (TaKaRa Biotechnology, Dalian, China). Relative expression levels were analyzed by using the 2−ΔΔCT method.
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bioRxiv - Biochemistry 2021Quote: ... falciparum cDNA followed by Ligation Independent Cloning into HindIII/KpnI-cleaved plasmid pOPIN F (82) using the In-Fusion HD EcoDry Cloning Kit (Takara Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Pooled samples were processed using SMARTer chemistry (SMART Seq® v4 Ultra® Low Input RNA Kit for Sequencing, Takara Clontech), according to manufacturer’s instructions to generate cDNA libraries for mRNA sequencing ...