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Citations for Takara Bio :
4101 - 4150 of 5255 citations for Rat Myristoylated Alanine Rich C Kinase Substrate MARCKS ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Deletion of the predicted helix motif in the hCAP-H N-tail was performed using PrimeSTAR Mutagenesis Basal Kit (TaKaRa). Primers used in the deletion were as follows ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was quantified using Naodrop and 1μg RNA was used for cDNA synthesis using commercial cDNA preparatory kit (Takara 6110A). For gene analysis the cDNA was amplified using gene specific primers by qPCR with Takara 2X SYBR Green Mix (Takara RR420A ...
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bioRxiv - Immunology 2022Quote: ... All resulting RNA was used as an input for complementary DNA synthesis using the SMART-Seq v4 kit (Takara Bio) and 10 cycles of PCR amplification ...
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bioRxiv - Genetics 2022Quote: ... total RNA extraction and separation of residual DNA contamination were excuted using Plant RNA Extraction Kit (TaKaRa MiniBEST, kyoto, Japan), and RNase-free DNase I (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2022Quote: ... The cloNAT cassette was amplified (LJ42 and LJ43) and inserted into the XhoI site in front of the Nse3 gene using the In-fusion cloning kit (Takara). A 650bp-long genomic sequence (upstream of Nse3 ...
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bioRxiv - Immunology 2022Quote: 150 tuft cells were sorted as CD45lo EpCAM+SigF+CD24+ directly into lysis buffer from the SMART-Seq v4 Ultra Low Input RNA Kit (Takara) and cDNA was generated following manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and cloned into SacI- and Xho-digested pGL3-Promoter vector using the Infusion®HD Cloning kit (Takara Bio #639650). Elements were cloned upstream of the sv40 promoter within the pGL3-Promoter vector ...
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bioRxiv - Microbiology 2022Quote: ... The amplified fragment was cloned into the BamHI site of pET-21a(+) using In-Fusion HD cloning Kit (Takara Bio), and the NotI fragment of the resulting plasmid was then inserted in pJB861 to generate pJBacv ...
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bioRxiv - Microbiology 2022Quote: ... total RNA underwent reverse transcription using a PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) (RR047A, Takara). The Bio-Rad iQ5 system was used to perform a qPCR procedure with the Hieff® qPCR SYBR Green Master Mix (No Rox ...
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bioRxiv - Immunology 2022Quote: ... selected RNAs from cDCs were used to amplify the cDNA using the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech-Takara). Amplified (1 ng ...
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bioRxiv - Immunology 2022Quote: ... Libraries for mRNA sequencing were prepared starting from 10 ng tot RNA for each sample by using the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech-Takara). All samples were sequenced on an Illumina NextSeq 550 at an average of 17,5 million 75-bp single-end reads.
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bioRxiv - Microbiology 2022Quote: ... and E484Q mutations of the SARS-CoV-2 spike protein were determined by qPCR using mutation detection kits purchased from Takara Bio (Shiga ...
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bioRxiv - Molecular Biology 2022Quote: Y1H library screening was performed by using the Matchmaker Gold Yeast One-Hybrid Library and Screening kit (Clontech,CA, USA). The promoter fragments of CaPR1 were inserted in the pAbAi vectors ...
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bioRxiv - Systems Biology 2022Quote: ... cDNA was synthesized from 1 μg of total RNA using the PrimeScript RT reagent kit (Perfect Real Time) (Cat No RR037A, TaKaRa) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... First-strand cDNA was synthesized from 1 μg of total RNA using the PrimeScript™ RT Master Mix Kit (Takara). SYBR Premix Ex Taq™ kit (Takara ...
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bioRxiv - Plant Biology 2022Quote: ... pOsSHR2::OsSHR2 or pOsPIN5c::OsPIN5c and inserted into the KpnI digested vector pCam23A (Sun et al., 2016) backbone by using the In-Fusion kit (Clontech), with primers are listed in Supplementary Table 4.
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bioRxiv - Molecular Biology 2023Quote: ... The ORF of zebrafish znf598 (ENSDARG00000014945) was amplified by RT-PCR and cloned into pCS2+ via XhoI/XbaI restriction sites using DNA Ligation Kit (TAKARA). To generate znf598 point mutation in ORF (znf598 C13/16A) ...
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bioRxiv - Molecular Biology 2023Quote: ... wild-type ORF was amplified using point mutated primers and cloned into pCS2+ via XhoI/XbaI restriction sites using DNA Ligation Kit (TAKARA). The ORFs of zebrafish etf1b (ENSDARG00000043976 ...
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bioRxiv - Molecular Biology 2023Quote: ... The ORF of zebrafish rpl36 (ENSDARG00000100588) was amplified by RT-PCR and cloned into pCS2+ via EcoRI/XhoI restriction sites using DNA Ligation Kit (TAKARA). The ORF of zebrafish znf598 (ENSDARG00000014945 ...
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bioRxiv - Plant Biology 2023Quote: ... and converted to cDNA using the SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing (Takara Bio), following the manufacturer’s instruction ...
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bioRxiv - Plant Biology 2022Quote: ... which was then cloned into the Aor51HI site in pUGW2 35S 97 using the In-Fusion cloning kit (TaKaRa Bio). The 2.5-kb HindIII-SacI fragment in the resulting plasmid ...
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bioRxiv - Microbiology 2023Quote: ... The resultant fragments were cloned into the EcoRI and NotI recognition sites of pCAG neo using HD Cloning Kit (TaKaRa). All recombinant proteins were fused with a his-tag and expression was confirmed using an anti-his tag antibody.
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bioRxiv - Microbiology 2023Quote: ... and were quantified by real-time RT-PCR using One Step TB Green PrimeScript RT-PCR Kit II (Perfect Real Time) (TaKaRa) and QuantStudio 3 Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... cDNAs were prepared from 1 ng of total RNA using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (TaKaRa) according to the manufacturer guidelines for cDNA synthesis and amplification ...
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bioRxiv - Microbiology 2022Quote: ... Ct values were obtained from PCR tests conducted as administrative tests at the Toyama Institute of Health using the SARS-CoV-2 direct detection RT-qPCR test kit from Takara Bio Inc ...
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bioRxiv - Microbiology 2022Quote: ... The cDNA was acquired by using the Prime Script RT reagent kit with genomic DNA (gDNA) eraser (TaKaRa, Beijing, China). The SYBR Premix Ex Taq II kit (TaKaRa ...
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bioRxiv - Neuroscience 2023Quote: 10 ng RNA from each worm sample was used as input for cDNA synthesis using a SMART-Seq v4 Ultra Low Input RNA kit (Takara). Sequencing libraries were prepared from 500 pg of cDNA with a Nextera XT DNA Library Prep kit (Illumina) ...
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bioRxiv - Neuroscience 2022Quote: ... the BamHI/BsrGI digested PCR product was ligated to the linearized backbone using the DNA Ligation Kit - Mighty Mix (TaKaRa) resulting pPB-ef1a-NLS-EGFP-T2A-puro.
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bioRxiv - Neuroscience 2022Quote: ... These guides were individually cloned into pAAV-U6-sasgRNA-CMV-mCherry-WPREpA at the BstXI and XhoI restriction enzyme sites using the In-Fusion HD cloning kit (Clontech). rAAV vectors were generated using similar plasmids and cloning methods as was referenced in (Matharu et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... The titer of harvested lentivirus was established using the Lenti-X RT-qPCR Titration Kit (Takara Bio, Cat. no. 631235). A representative calibration plot and LV tittering of pAIO is added as Supplementary data S4.
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bioRxiv - Molecular Biology 2022Quote: ... Probes for FLuc (500 bp) were generated using gel-purified PCR amplicons containing GFP sequence and a BcaBEST Labeling kit (Takara) and [α-32P]-dCTP (PerkinElmer) ...
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bioRxiv - Molecular Biology 2022Quote: ... Amplified mTurbo and FLAG-cyp40 fragments were introduced into XbaI site of pUASp-K10-attB vector using In-Fusion HD Cloning Kit (Takara). To generate UASp-mTurbo-FLAG-cyp40deltaTPR ...
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bioRxiv - Microbiology 2022Quote: ... RNA was then purified and resuspended with SMART-Seq v4 Ultra Low Input RNA Kit (Takara Bio Inc., Shiga, Japan) according to the manufacturer’s instructions for mRNA amplification using 5’ template switching polymerase chain reaction (PCR) ...
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bioRxiv - Molecular Biology 2022Quote: ... Detection relied on a 32P-labeled DNA probe specific for GFP sequence synthesized with a random priming labeling kit (Takara) followed by exposure to film and development as described (43).
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bioRxiv - Microbiology 2022Quote: ... The resulting mutant BAC was electroporated into NEB 10-beta cells and purified using the Nucleobond BAC 100 kit (Takara).
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bioRxiv - Molecular Biology 2022Quote: ... Libraries for RNA sequencing were prepared from 5 ng RNA/sample using the SMARTer Stranded Total RNA-Seq Kit v2 -Pico Input Mammalian (Takara) according to the manufacturer’s instructions using 12 PCR cycles for amplification ...
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bioRxiv - Molecular Biology 2022Quote: ... The freshly extracted plasmids were transformed into yeast strain Y2H gold by using a yeast transformation kit (TaKaRa, Beijing, China) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... were subjected to RNA-seq library preparation using SMARTer Stranded Total RNA-Seq Kit – Pico Input Mammalian (Cat. No. 635005, TAKARA). All the libraries were analysed through a Bioanalyzer for quality control ...
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bioRxiv - Physiology 2022Quote: ... cDNA was generated from 200ng of RNA using PrimeScript RT reagent Kit with gDNA Eraser (Takara Bio; Cat. No. RR047A) according to the manufacturer’s directions ...
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bioRxiv - Microbiology 2022Quote: ... A CVB3 genome encoding a NanoLuc luciferase (NLuc) reporter was generated using the In-Fusion HD Cloning Kit (Takara, 638909). NanoLuc luciferase was cloned from the pLenti6.2-Nanoluc-ccdB ...
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bioRxiv - Microbiology 2022Quote: ... was used as the template for real-time RT-PCR performed according to the manufacturer’s protocol using the One Step TB Green PrimeScript PLUS RT-PCR kit (Takara-Bio) and the following primers ...
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bioRxiv - Microbiology 2022Quote: ... The viral RNA copy number was standardized with a SARS-CoV-2 direct detection RT-qPCR kit (Takara, Cat# RC300A). Fluorescent signals were acquired using QuantStudio Real-Time PCR system (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... 1 ng of total RNA was pre-amplified with the SMARTer Ultra Low Input kit v4 (Clontech, Mountain View, CA) per manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2022Quote: ... as described previously.15 Alkaline phosphatase (ALP) staining was also performed for pluripotency characterization using TRACP & ALP double-stain Kit (Takara) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Transient transfection was performed with the use of HilyMax liposome transfection reagent (Dojindo Laboratories) or CalPhos Mammalian Transfection Kit (Clontech). Unless otherwise noted ...
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bioRxiv - Microbiology 2024Quote: ... The RNA samples were then reverse transcribed employing a One-Step SYBR PrimerScript reverse transcription (RT)-PCR kit (TaKaRa, Japan). Nested PCR conditions were as follows ...
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bioRxiv - Molecular Biology 2024Quote: ... the annealed oligos were ligated into the digested lentiCRISPRv2 backbone using the DNA ligation kit (Mighty Mix) (Takara, Cat# 6023). Afterward ...
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bioRxiv - Microbiology 2024Quote: ... Total RNA was extracted using RNApure FFPE Kit (CWBIO, Jiangsu, China) and then treated with DNase I (TaKaRa, Kyoto, Japan) to remove DNA contaminants ...
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bioRxiv - Plant Biology 2024Quote: Rapid amplification of cDNA ends (5’ RACE) was used to determine the transcripts of the RB gene using the SMARTer RACE cDNA Amplification Kit (Clontech) according to the protocol ...
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bioRxiv - Biochemistry 2024Quote: ... The RNA was quantified and 1.5 μg of RNA was used to prepare cDNA using PrimeScript 1st strand cDNA Synthesis Kit (Takara Bio) and random hexamer primer ...