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Citations for Takara Bio :
3951 - 4000 of 6421 citations for Rat Epithelial membrane protein 1 EPN3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... cDNA library preparations were conducted using the SMART-Seq v4 Ultra Low Input kit from TaKaRa (Cat. # 634894) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... RNA was amplified using SMARTer® Universal Low Input RNA Kit for sequencing (Takara, Mountain View, CA, USA). Ribosomal RNA was depleted using RIboGone™-Mammalian rRNA removal kit (Takara ...
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bioRxiv - Molecular Biology 2021Quote: ... The respective PCR products were then cloned into pcDNA5_FRT_TO_3xFlag(N) using In-Fusion® HD Cloning Kit (Cat. No. 639650, Takara).
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bioRxiv - Microbiology 2021Quote: ... Reverse transcription was done using PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) (Takara, #RR047A). qPCR reaction was prepared by TB Green® Premix Ex Taq™ (Tli RNaseH Plus ...
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bioRxiv - Systems Biology 2021Quote: ... Ribosomal RNA was depleted using the RiboGone – Mammalian Kit protocol (Cat #634847, Takara Bio USA, Mountain View, CA). The SMARTer Universal Low Input RNA Kit protocol v2 (Cat #634938 ...
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bioRxiv - Microbiology 2020Quote: ... converted to single stranded cDNA and amplified using the SMARTer PCR cDNA Synthesis Kit (Takara Bio, Kusatsu, Japan), and IsoSeq libraries were constructed with equimolar cDNA fractions (0.5X and 1X ...
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bioRxiv - Cell Biology 2021Quote: Total RNA was isolated from wild-type or transiently transfected cells with MiniBEST Universal RNA Extraction Kit (Takara), an additional DNase I (NEB ...
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bioRxiv - Cell Biology 2020Quote: ... 100 ng of total RNA was reverse-transcribed using the PrimeScript RT Reagent Kit (Takara Bio, Shiga, Japan) and a T100 thermal cycler (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2021Quote: ... total RNA was extracted using TRIzol reagent (Fermentas) and reverse transcribed using the mRNA Selective PCR Kit (TaKaRa) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: RNA was quantified (by Nanodrop) and retrotranscribed to cDNA (PrimeScript™ RT-PCR Kit - Takara Bio Cat. # RR014A). Real time PCR was performed with KAPA SYBR® FAST qPCR Master Mix (2X ...
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bioRxiv - Cell Biology 2021Quote: Total genomic DNA of HeLa cells was extracted after irradiation using Total genomic DNA extract kit (TAKARA, China). Human nuclear GAPDH gene (forward ...
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bioRxiv - Biochemistry 2022Quote: ... AAV-GFP and AAV-Mafa were purified with the AAV pro Extraction Solution Kit (6235, Takara, Shiga, Japan), and the titers were determined with a Quick Titer AAV Quantification Kit (VPK-145 ...
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bioRxiv - Physiology 2022Quote: ... cDNA was synthesized using 2 μg RNA using the PrimeScript™ RT reagent Kit with gDNA Eraser (TaKaRa). Gene specific primers for quantitative real-time PCR (qRT-PCR ...
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bioRxiv - Plant Biology 2022Quote: ... and 3’ UTR region was cloned into the PacI site in pZH_OsU6sgRNA_SpCas-wt (Endo et al., 2019) using an In-Fusion HD cloning kit (Takara). Two pairs of 20-nt annealed oligonucleotides targeting exon 4 of the OsSOG1 gene were cloned into the BbsI site of the pU6gRNA-oligo (pU6::sgOsSOG1-2 and pU6::sgOsSOG1-3 ...
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bioRxiv - Microbiology 2022Quote: ... a one-step reverse transcription-PCR (RT-PCR) assay was conducted with a PrimeScript kit (Takara, Tokyo, Japan), and Viral genomic terminal sequences were determined using commercial 5’ and 3’ RACE (rapid amplification of cDNA ends ...
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bioRxiv - Molecular Biology 2022Quote: ... The resulting cDNA was subjected to relative quantitative PCR using a SYBR Premix Ex Taq™ kit (TaKaRa) on a Roche LightCycler 480 real-time PCR machine ...
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bioRxiv - Biophysics 2022Quote: ... 3’-RACE-Ready cDNA template was synthesized using a SMARTer® RACE 5’/3’ Kit (Takara Bio, USA) and subsequently used to amplify 3’ end sequences of R ...
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bioRxiv - Cell Biology 2022Quote: RNA was extracted at each time point using the Nucleospin RNA XS kit from Clontech (cat. num. 740902.250). RNA quality was assessed using a NanoDrop and then Bioanalyzer was used to quantify RNA amount ...
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bioRxiv - Cell Biology 2022Quote: ... and inserted between BamHI and EcoRI restriction sites by using restriction enzymes and a DNA ligation kit (TaKaRa). Constructs of hCAP-H2 harboring charge-reversal mutations in the BC1 and BC2 regions (H2-BC1D ...
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bioRxiv - Plant Biology 2021Quote: ... First-strand cDNA was synthesized from 500 ng total RNA using the PrimeScript RT reagent kit (Takara, Japan). Quantitative RT-PCR (qRT-PCR ...
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bioRxiv - Neuroscience 2020Quote: ... For FANS RNA-seq library preparation (SMARTer Stranded Total RNA-Seq Kit Pico Input Mammalian, Clontech Laboratories, 635005), 2952pg of total nuclear RNA were amplified into cDNA with fragmentation times of 2.5 min for autopsy and 3.5 min for TLE cases (14 cycles total amplification for all) ...
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bioRxiv - Bioengineering 2021Quote: ... The PCR amplified CLIPtag were then ligated with the digested vectors using In-Fusion HD Cloning kit (Clontech). The plasmids pSNAPf-Clathrin-15 and pSNAPf-LMNA were directly purchased from UCSF Nikon Imaging Center ...
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bioRxiv - Immunology 2020Quote: ... q-RT-PCR was performed using the One Step PrimeScript™ RT-PCR Kit (Perfect Real Time; TaKaRa). The primers used for q-RT-PCR were selected to be specific for the E and ORF1ab sequences in the SARS-CoV-2 genome (Table 1) ...
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bioRxiv - Plant Biology 2021Quote: ... Isolated RNA was used as template for cDNA synthesis using PrimeScript RT Reagent Kit (Takara Bio, Kusatsu, Japan). Transcript expression was quantified by qRT-PCR using iQ SYBR Green supermix (Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2020Quote: ... The purified mixture of PCR products was cloned into pMD20-T vector using Mighty TA-cloning Kit (TaKaRa). The 119 clones for AAVS-tid GTC_70-107 (+ ...
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bioRxiv - Neuroscience 2020Quote: ... Genomic titer was determined using the Lenti-Z qRT-PCR Titration Kit according to manufacturer’s instructions (Takara #631235). Smaller scale virus prep for sgRNAs and shRNAs was performed through a similar transfection in a 12-well culture plate ...
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bioRxiv - Immunology 2020Quote: ... mRNA library preparation was performed following manufacturer’s recommendations (SMART-Seq v4 Ultra Low Input RNA Kit, TAKARA Bio). Final samples pooled library prep was sequenced on Nextseq 500 ILLUMINA with HighOutPut cartridge (2×400Millions of 75 bases reads) ...
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bioRxiv - Microbiology 2021Quote: DNA was extracted from 250 mg of sponge sample using the Nucleospin Soil DNA extraction kit (Takara Bio) and was submitted to the Baylor College of Medicine’s Alkek Center for Metagenomics & Microbiome Research for high-throughput sequencing ...
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bioRxiv - Plant Biology 2021Quote: ... The PCR products were cloned into the modified pET28 vector using the In-Fusion HD Cloning Kit (Clontech) to generate pET28M-MpaKAI2A and pET28M-MpaKAI2B ...
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bioRxiv - Molecular Biology 2022Quote: ... poly-A libraries were prepared using the SMARTer ThruPLEX DNA-seq Kit (R400675, Takara, Mountain View, CA, USA). Each library was normalized to 1.5 nM ...
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bioRxiv - Cell Biology 2022Quote: ... Titer of the lentiviruses was measured with a lentivirus titration kit (Takara Bio USA, San Jose, CA, USA). The same multiplicity of infection (MOI ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA libraries were prepared with the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Takara, 634413). All samples were sequenced on Illumina NextSeq500 (Illumina).
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bioRxiv - Developmental Biology 2022Quote: ... 500 ng of total RNA was reverse-transcribed into cDNAs using the PrimeScript RT Reagent Kit (TaKaRa, RR037A) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... IGK and IGL 5’RACE AIRR-seq libraries were generated using the SMARTer Mouse BCR Profiling Kit (Takara Bio ...
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bioRxiv - Cancer Biology 2022Quote: ... Base substitutions were induced in the pMXs vector with the PrimeSTAR® Mutagenesis Basal Kit (TaKaRa Bio Inc.) before transfection into Plat-E cells to create ALK-TKIs resistant mutants.
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bioRxiv - Biochemistry 2022Quote: ... and the DNA was extracted and then DNA libraries were prepared with Smarter Thruplex DNAseq Kit (Clontech, R400674) following the manufacturer’s protocols with amplification for 10-13 circles ...
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bioRxiv - Microbiology 2022Quote: ... Release of LDH into the culture supernatant was quantified after infection using an LDH Cytotoxicity Detection Kit (Clontech). LDH release was normalized to mock-infected cells and cells treated with 1% Triton to establish maximum LDH release ...
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bioRxiv - Developmental Biology 2022Quote: Purified total RNA was reverse transcribed into cDNA by PrimeScript™ 2 1st strand cDNA Synthesis Kit (TaKaRa). The target gene fragments (Brachyury ...
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bioRxiv - Neuroscience 2019Quote: ... Total RNA from the tissue samples was extracted using a TaKaRa MiniBEST Universal RNA Extraction Kit (TaKaRa, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... 2.4 µg of clean RNA was reverse-transcribed to cDNA using Takara RNA PCR kit (Takara, Kyoto, Japan). Gene-specific primers (Table S3 ...
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bioRxiv - Neuroscience 2019Quote: ... Single stranded cDNA from the total RNA was synthesized with a RT-PCR kit (Clontech, Mountain View, CA) according to the kit’s instructions ...
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bioRxiv - Bioengineering 2019Quote: ... and reverse-transcribed by a PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) (TAKARA, Japan). qPCR was performed with SYBR Premix Ex Taq™ II (Tli RNaseH Plus ...
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bioRxiv - Molecular Biology 2019Quote: ... Libraries were prepared with 50 ng starting material as instructed by the ThruPLEX DNA-seq kit (R400427, Takara). Amplification was performed with 5 rounds of PCR ...
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bioRxiv - Neuroscience 2019Quote: ... and cloned into pEX-A (Eurofins Genomics, USA) using an In-Fusion HD Cloning Kit (Takara Bio, Japan). The cloned plasmids were transformed into Stellar competent cells (Takara Bio ...
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bioRxiv - Physiology 2019Quote: ... cDNA synthesis and PCR amplification were performed using PrimeScript First-Strand cDNA Synthesis kit (TaKaRa Bio, Tokyo, Japan) with 1 μg total RNA plus random hexamers ...
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bioRxiv - Immunology 2019Quote: ... using the manufacturers protocol and amplified using SMARTer Ultra Low Input RNA kit for sequencing (version 4, Clontech). Sequencing libraries were generated using TruSeq Nano DNA Sample Preparation kits (Illumina) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and 1000 ng of total RNA were reverse transcribed using the PrimeScript RT-qPCR Kit (TaKaRa, Cat#RR037B). At least four independent biological replicates were collected and analyzed ...
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bioRxiv - Cancer Biology 2019Quote: ... Dox-inducible vectors were generated according to a publicly available protocol60 using In-Fusion HD Cloning Kit (Clontech) (Supplementary Table 5) ...
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bioRxiv - Molecular Biology 2019Quote: ... Then the mCherry cassette was replaced by Puro or EGFP by In-Fusion® HD Cloning Kit (Clontech). To generate the enCRISPRi-LK sgRNA vector ...
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bioRxiv - Plant Biology 2019Quote: ... Real-time quantitative RT-PCR was performed with a PrimeScript II 1st strand cDNA Synthesis Kit (TaKaRa Bio) and SYBR Premix Ex Taq II (TaKaRa Bio) ...