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Citations for Takara Bio :
351 - 400 of 1129 citations for Transcription Factor SOX 10 SOX10 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... The single strand cDNA for 5’RACE was prepared by in vitro reverse transcription with avian myeloblastosis virus reverse transcriptase XL (Takara Bio) using total RNA (0.5 µg ...
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bioRxiv - Plant Biology 2021Quote: ... followed by reverse transcription using the PrimeScript™ RT Reagent Kit with gDNA Eraser (Perfect Real Time) (Takara Bio, Shiga, Japan). Quantitative PCR was conducted using the KAPA SYBR FAST qPCR Master Mix (2X ...
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bioRxiv - Molecular Biology 2022Quote: ... Total RNA were also extracted from in vitro samples of cultured GC and reverse transcription reactions were performed using the SMART PCR cDNA synthesis technology (Takara Bio.) according to the manufacturer’s procedure and as previously published 35 ...
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bioRxiv - Immunology 2022Quote: ... Total RNA was then subjected to genomic DNA (gDNA) elimination and reverse-transcription according to PrimeScript™ RT reagent kit protocols (Takara). Briefly ...
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bioRxiv - Plant Biology 2022Quote: ... . cDNA was synthesized by the use of PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) according to the reverse transcription protocol (Takara). The resulting cDNA was subjected to relative quantitative PCR using a SYBR Premix Ex TaqTM kit (TaKaRa ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA was synthesized by the use of PrimeScript™RT reagent Kit with gDNA Eraser (Perfect Real Time) according to the reverse transcription protocol (Takara). The resulting cDNA was subjected to relative quantitative PCR using a SYBR Premix Ex Taq™ kit (TaKaRa ...
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bioRxiv - Immunology 2021Quote: ... and the reverse transcription products were amplified using the Agilent-Strata gene MxReal-Time qPCR system with a PrimeScript™ RT Reagent Kit (Takara). Reverse transcription products were amplified using an Agilent-Strata gene MxReal-Time qPCR system with TB Green® Premix Ex Taq™ II (Tli RNaseH Plus ...
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bioRxiv - Genomics 2022Quote: ... Total RNA from SARS-CoV-2 infected lung or trachea was subjected to one-step real-time reverse transcription PCR using One-step PrimeScript RT-PCR kit (Takara, RR064B). Multiplex PCR was performed to detect SARS-CoV-2 nucleocapsid gene and mouse Actb gene ...
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bioRxiv - Cell Biology 2019Quote: ... from each ample was used to synthesize first-strand cDNA by reverse transcription using the PrimeScript™ RT reagent Kit with gDNA Eraser (Takara), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... and reverse transcription was further performed with 500 ng total RNA as initiation material with PrimeScriptTM RT Master Mix (TaKaRa, RR036A). qRT-PCR was conducted with 2 x S6 Universal SYBR qPCR Mix (NovaBio ...
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bioRxiv - Genomics 2021Quote: ... reverse transcription and cDNA preamplification were performed in the C1 Fluidigm system using the SMARTer PCR cDNA Synthesis Kit (Clontech 634926) according to manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... the in vitro Cas9 cleavage was performed using a previously amplified target pyrG amplicon following the steps of the Guide-it™ sgRNA In Vitro Transcription and Screening Systems User Manual (Takara Bio USA ...
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bioRxiv - Plant Biology 2022Quote: ... Plasmid vectors were linearized to generate templates for viral RNA in vitro transcription (T7 RNA Polymerase System, Takara Bio, Shiga, Japan). RNA1 to RNA3 were equally mixed to infect 3-week-old N ...
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bioRxiv - Developmental Biology 2023Quote: ... was eliminated by digesting with genomic DNA eraser buffer and cDNA was obtained by reverse transcription of RNA using PrimeScript RT reagent Kit with gDNA Eraser (Takara, RR047A). To measure the values of genes specifc primers ...
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bioRxiv - Developmental Biology 2023Quote: ... Reverse transcription and cDNA library preparation were performed with a SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech, 634888). cDNA was fragmented with a Covaris S 220 instrument (Covaris) ...
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bioRxiv - Cell Biology 2023Quote: was used to carry out reverse transcription (RT) reaction to obtain cDNA following the instruction of PrimeScript RT Reagent Kit (Takara, Japan). RT primers are specific to midRs ...
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bioRxiv - Neuroscience 2023Quote: ... Two micrograms of the RNA were subjected to reverse transcription with random hexamers and the PrimeScript™ 1st strand cDNA Synthesis Kit (TaKaRa). For semi-quantitative PCR ...
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bioRxiv - Immunology 2023Quote: ... reverse transcription and cDNA pre-amplification were performed using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech/Takara). cDNA was harvested and quantified with the Bioanalyzer DNA High-Sensitivity kit (Agilent Technologies) ...
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bioRxiv - Genomics 2023Quote: ... The same amount of input RNA for each batch was used for reverse transcription (RT) reactions with SMARTScribe™ Reverse Transcriptase (Takara) and oligo dT following the manufacturer’s manual ...
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bioRxiv - Developmental Biology 2023Quote: ... about 50 ng synthesized template DNA was in vitro transcribed using in vitro Transcription T7 Kit (for siRNA Synthesis) (Takara bio), treated with DNase I (Takara Bio) ...
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bioRxiv - Developmental Biology 2024Quote: ... genomic DNA removal and reverse transcription were carried out using the PrimeScript™ RT reagent kit with gDNA Eraser (Takara Bio).
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bioRxiv - Cell Biology 2024Quote: ... rat cerebral cortexes or fly brains using TRIzol and the reverse transcription was performed with PrimeScript™ RT Master Mix (Takara) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... Reverse transcription of 500 ng purified total RNA was performed by PrimeScript RT reagent kit with gDNA Eraser (Takara, Dalian, China). RT-qPCR was performed with SYBR green master mix (Takara ...
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bioRxiv - Molecular Biology 2024Quote: ... 500 ng of total RNA underwent reverse transcription to generate cDNA using the primeScriptTM RT Master Mix reagent kit (TaKaRa, Japan). Quantitative assessment of relative RNA expression was performed via RT-qPCR using SYBR Premix Ex TaqTM (TaKaRa ...
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bioRxiv - Immunology 2023Quote: ... Reverse transcription was performed on 1 µg total mRNA per sample using PrimeScript™ RT Reagent Kit with gDNA Eraser (Takara) according to the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2020Quote: ... An amount of 400 ng of the extracted RNA was subjected to reverse transcription by using a PrimeScript RT Reagent Kit (Takara, Shiga, Japan). With complementary DNA (cDNA ...
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bioRxiv - Molecular Biology 2019Quote: ... The same samples used for RNA sequencing were used for reverse transcription and synthesis of cDNA with PrimeScript RT Reagent Kit with gDNA Eraser following the instructions of the manufacturer (TaKaRa, Dalian, China). For designing the primers for qRT-PCR ...
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bioRxiv - Plant Biology 2019Quote: ... Two micrograms of total RNA and 200 ng of a random primer were used in reverse transcription (RT) through the cDNA synthesis kit (TaKaRa, Dalian, China) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2022Quote: 500 ng of total RNA extracted from cell culture were subjected to reverse transcription, using ISOGEN II (NIPPON GENE, Tokyo, Japan) and PrimeScript RT reagent Kit (TaKaRa, Kusatsu, Japan). The resulting cDNA was subjected to real-time PCR analysis using glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Microbiology 2021Quote: ... We used the 1st cDNA synthesized by reverse transcription as a template and used the Premix Ex Taq™ Hot Start Version (Takara, Japan) to amplify the full-length sequence of the EgGLUT1-ss gene ...
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bioRxiv - Microbiology 2019Quote: ... Reverse transcription (RT) was performed with 200 ng total RNA using the Prime Script RT reagent Kit with gDNA Eraser (Takara, Dalian, P.R.China) following the manufacturer’s instructions.
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bioRxiv - Genetics 2020Quote: ... First-round reverse transcription PCR (RT-PCR) was conducted by using PrimeScript One Step RT-PCR Kit Ver.2 (TaKaRa, Dalian, China). A 10 μL reaction mixture contained 5 μL of 2 × 1 Step Buffer ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The Rdl PCR-product was subcloned into the transcription vector pTB-207 38 using the In-Fusion® HD Cloning Kit (Clontech™) and cRNAs were synthesized with the T7 mMessage mMachine kit (Ambion™) ...
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bioRxiv - Immunology 2020Quote: ... the reverse transcription (RT) reaction was performed in a two-step method following the PrimeScript™ II Reverse Transcriptase kit protocol (Takara, Japan). For the first step ...
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Rac1, Rac3 GTPases and TPC2 are required for axonal outgrowth and migration of cortical interneuronsbioRxiv - Neuroscience 2022Quote: ... Total RNA was subjected to reverse transcription according to the protocol of Prime Script 1st Strand cDNA Synthesis kit (Takara Bio USA). Real time PCR analysis was performed using a Step One Plus real-time PCR system (Applied Biosystems ...
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bioRxiv - Cancer Biology 2022Quote: ... For reverse transcription 2 µg RNA were translated into cDNA using the “RNA to cDNA EcoDry” Kit (Takara Bio USA, Kusatsu, Japan) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... were synthesized in vitro transcription based on the corresponding cDNAs inserted into the pGEM-T easy vector with T7 RNA polymerase (Takara, Beijing, China) as previously described (22) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The PCR products were used as templates to synthesize specific dsRNA using the in vitro Transcription T7 Kit (TaKaRa Biotechnology, Dalian, China) (dsRNA-Vitro ...
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bioRxiv - Plant Biology 2023Quote: Total RNAs from inflorescences or seedlings were treated with DNase I followed by reverse transcription using PrimeScript™ II 1st Strand cDNA Synthesis Kit (TAKARA, 6210A) with oligo-d(T ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed in triplicates using TB Premix Ex Taq II (Takara Bio, #RR390A, Japan) in a QuantStudio 6 Flex Real-Time PCR System using QuantStudio Real-Time PCR Software (both Applied Biosystems ...
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bioRxiv - Molecular Biology 2024Quote: ... The RNA extracted from the gonads underwent reverse transcription after concentration adjustment with the PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) (Takara, Japan).
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bioRxiv - Neuroscience 2024Quote: ... Reverse transcription was performed with 500 ng of total RNA and PrimeScript™ RT Master Mix (Perfect Real Time) (Takara, Tokyo, Japan). Reverse transcription PCR (RT-PCR ...
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bioRxiv - Microbiology 2023Quote: ... anti-GAPDH antibody (Clontech) at a 1/2500 dilution ...
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bioRxiv - Genomics 2020Quote: ... 10 μL of Mixture (TaKaRa, Japan), 1 μL upstream primers (10 μmol/L ...
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bioRxiv - Microbiology 2022Quote: ... 10 μL of Clone AMP (Takara), and 300 nM each of primers ks1 and mf83 ...
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bioRxiv - Developmental Biology 2019Quote: ... 10 U μl–1 SMARTScribe (Clontech) and 10 U μl–1 RNASin plus (Promega) ...
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Differential turnover of Nup188 controls its levels at centrosomes and role in centriole duplicationbioRxiv - Cell Biology 2019Quote: ... 10% Tet system approved FBS (Takara), 100 units/ml penicillin and 100 μg/ml streptomycin (Thermo Fisher Scientific) ...
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bioRxiv - Synthetic Biology 2021Quote: ... and 10% Fetal Bovine Serum (Clontech). Cells were grown at 37°C with 5% CO2 and passaged using 0.05% Trypsin-EDTA solution (Thermo Fisher ...
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bioRxiv - Synthetic Biology 2020Quote: ... 10% Tet Approved FBS (Clontech Laboratories), and 1X Penicillin/Streptomycin (Gibco) ...
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bioRxiv - Neuroscience 2019Quote: ... 10-15 µg of EGFP (Clontech) was coated onto 6-8 mg of 1.6 µm gold beads.