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Citations for Takara Bio :
351 - 400 of 808 citations for Saporin Chicken Polyclonal affinity purified Alexa488 labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... The transposed DNA fragments were purified by TaKaRa MiniBEST DNA Fragment Purification Kit (No ...
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bioRxiv - Biochemistry 2021Quote: ... Protein was purified using amylose-agarose resin (Clontech) and eluted in 10 mM maltose ...
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bioRxiv - Microbiology 2021Quote: ... and protein purified using TALON resin (Takara Bio) using standard protocols in 50 mM phosphate buffer pH7.4 containing 300mM NaCl.
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bioRxiv - Molecular Biology 2020Quote: ... The complex was purified using TALON beads (Clontech) after nucleic acid precipitation using 0.05% of PolyEthylImine (PEI ...
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bioRxiv - Immunology 2019Quote: ... Fabs were purified using Cobalt-TALON resin (Takara) followed by gel filtration chromatography on Superdex 200 Increase (GE Healthcare ...
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bioRxiv - Microbiology 2022Quote: ... proteins were purified using Talon resin (Takara Bio) by resuspending the cell pellet in lysis buffer (50 mM phosphate buffer and 300 mM NaCl ...
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bioRxiv - Microbiology 2022Quote: ... and purified with NucleoZOL (Takara Bio, Göteborg, Sweden). Ribosomal RNA depletion was performed with the NEBNext Bacteria kit (NEB ...
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bioRxiv - Developmental Biology 2020Quote: ... dissected calvaria from 9SL or 11SL fish were labeled with antibodies against mCherry (Takara, 1/100), GFP (Roche ...
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Constitutive and conditional epitope-tagging of endogenous G protein coupled receptors in DrosophilabioRxiv - Neuroscience 2023Quote: Myristoylated TdTomato (myr::TdTom) was labeled with 1:500 rabbit anti-DsRed (Takara Cat# 632496, RRID:AB_10013483)
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bioRxiv - Immunology 2021Quote: ... The TSO was designed with two isodeoxynucleotides at the 5’ end to prevent TSO concatemerization and three riboguanosines at the 3’ end for increased binding affinity to the appended deoxycytidines (property of the Takara reverse transcriptase) (56 ...
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bioRxiv - Microbiology 2021Quote: ... Cell lysates were prepared using a French press and susequent protein purification accomplished using Talon metal affinity resin (TaKaRa Bio USA, Inc.) as described by the manufacturer ...
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bioRxiv - Biophysics 2023Quote: ... Cell debris was pelleted down and the supernatant was run on a 2 mL column volume (CV) TALON cobalt affinity resin (Takara Bio #635504) equilibrated in CoWB/TCEP (100 mM NaCl (Sigma-Aldrich 746398) ...
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bioRxiv - Microbiology 2023Quote: ... The cleared lysate was loaded onto a 25 mL free-flow gravity column (GeneFlow) packed with 3 ml TALON® Metal Affinity Resin (Takara Bio), washed with 10 column volumes (CV ...
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bioRxiv - Developmental Biology 2022Quote: c-Kit-EGFP fusion protein: chicken c-Kit cDNA was inserted into multiple cloning site of pEGFP-N1 Vector (Clontech, #6085-1) including EGFP sequences using a primer set ...
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bioRxiv - Cell Biology 2021Quote: ... 1982) or a commercial antibody against dsRed that detects mCherry (Living Colors DsRed Polyclonal Antibody, Clontech). After 3 consecutive 5-min washes in PBS ...
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bioRxiv - Cell Biology 2019Quote: ... 2016) using anti-GFP (Living Colors A.v. peptide antibody, rabbit polyclonal, 1 mg/ml; TakaraBio/Clontech) primary antibody and horseradish peroxidase linked anti-rabbit IgG (from donkey ...
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bioRxiv - Neuroscience 2023Quote: ... a mix of an mCherry-DsRed rabbit polyclonal antibody (Living Colors-Clontech Antibody 632496, 1:1000) and a mouse monoclonal antibody against oxytocin (P38 ...
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bioRxiv - Biochemistry 2021Quote: ... After sonication and centrifugation (20 000 g, 1 h, 4 °C) the supernatant was mixed with Talon Metal Affinity Resin (Takara Bio USA, Inc.). After 1 hour incubation at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... and was then incubated with 2 mL TBS pH 8.0 pre-equilibrated Talon® Metal Affinity resin during 2 h at 4°C with permanent mixing (Takara Bio, Kusatsu, Japan). The resin was washed with 10 volumes of TBS pH 8.0 buffer containing 0.1% Triton X-100 and 10 mM imidazol (both from Sigma) ...
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bioRxiv - Biochemistry 2022Quote: ... An oligonucleotide probe against snoRNA58 labeled with [γ-32P] ATP by T4 polynucleotide kinase (Takara, cat# 2021A) was used as a loading control ...
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bioRxiv - Biophysics 2021Quote: ... lysed and purified over Talon Superflow resin (Takara Clontech). The His6-tag was removed by thrombin cleavage and the proteins further purified with a HiLoad 16/60 Superdex 75 prep grade column (GE Healthcare ...
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bioRxiv - Biophysics 2021Quote: ... lysed and purified using Talon Superflow resin (Takara Clontech). Further ...
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bioRxiv - Biophysics 2021Quote: ... lysed and purified over Talon Superflow resin (Takara Clontech). The His6-tag was removed by thrombin cleavage and the proteins further purified with a HiLoad 16/60 Superdex 75 prep grade column (GE Healthcare ...
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bioRxiv - Biophysics 2021Quote: ... lysed and purified using Talon Superflow resin (Takara Clontech). Further ...
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bioRxiv - Molecular Biology 2022Quote: ... and purified by MPG streptavidin beads (Takara, cat. 6124A) followed by RNAse (Promega ...
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bioRxiv - Biophysics 2020Quote: ... The protein was initially purified using Talon resin (Clontech) with a linear gradient of 50 to 200 mM imidazole ...
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bioRxiv - Cell Biology 2021Quote: RNA was purified with a NucleoSpin RNA kit (Takara) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... The assembled full-length inserts were gel purified (Takara), digested with EcoRI (NEB) ...
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bioRxiv - Microbiology 2023Quote: ... recombinant AAVs were purified using an AAVpro Concentrator (TaKaRa) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... and purified overnight using Lenti-X Concentrator (Takara Bio). Concentrated lentiviral pellets were then resuspended in complete CHO ts20 medium and either used immediately or snap frozen in liquid nitrogen and stored at −80°C.
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bioRxiv - Neuroscience 2023Quote: ... the samples were incubated in primary antibody (chicken anti-GFP, Abcam #13970, 1:2000, rabbit anti-DsRed, Takara Bio Clontech #632496, 1:200) for 24 – 48 hours in the dark while shaking at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... the samples were incubated in primary antibody (chicken anti-GFP, Abcam #13970, 1:2000, rabbit anti-DsRed, Takara Bio Clontech #632496, 1:200) for 24 – 48 hours in the dark while shaking at 4 °C ...
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bioRxiv - Biophysics 2019Quote: ... cells bearing the corresponding plasmids and were purified according to.63 Partially purified lysates were loaded onto equilibrated cobalt His-TALON columns (Clontech, Mountain View, CA). The column was washed with 40 to 50 volumes of wash buffer (50 mM NaH2PO4 ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were incubated with a diluted primary antibody: rabbit polyclonal anti-DsRed at 1:1000 dilution (Takara Biomedical Technology ...
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bioRxiv - Immunology 2023Quote: Primary antibodies used for imaging include Living Colors anti-DsRed Rabbit Polyclonal Pan Antibody (1:500; TaKaRa), Chicken Polyclonal anti-GFP (1:300 ...
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bioRxiv - Bioengineering 2023Quote: ... Protease inhibitors and 10% glycerol were added to the supernatant before loading it onto 1 mL of Talon metal affinity resin (Clontech, Mountain View, CA, #635502). After a 20 mL buffer wash ...
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bioRxiv - Microbiology 2020Quote: ... iRBDs were purified by passage over Co-NTA agarose (Clontech) followed by gel filtration chromatography on Superdex 200 (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... Clarified supernatants were purified in batch over Talon resin (Takara) prior to buffer exchanging into 20mM Tris pH8 150mM NaCl and flash freezing.
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bioRxiv - Cell Biology 2020Quote: ... The solubilized protein was purified by Talon-resin (Clontech/Takara) using the hexa-histidine-tag fused at the N-terminus of the protein ...
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bioRxiv - Cell Biology 2020Quote: ... The solubilized protein was purified by Talon-resin (Clontech/Takara) using the hexa-histidine-tag fused at the N-terminus of the protein ...
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Biosynthesis of Circular RNA ciRS-7/CDR1as Is Mediated by Mammalian-Wide Interspersed Repeats (MIRs)bioRxiv - Molecular Biology 2019Quote: ... Purified RNA was reverse-transcribed using PrimeScript II (Takara Bio) with oligo-dT and random primers ...
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bioRxiv - Microbiology 2019Quote: ... The resulting enzyme was then purified by Talon chromatography (Clontech) and underwent iron-sulfur cluster reconstitution using previously published protocols 26,30,31.
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bioRxiv - Cell Biology 2019Quote: ... Protein was purified on a His60 Ni Superflow column (Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... The solubilized fragment was purified by Talon-resin (Clontech/Takara) using the hexa-histidine-tag fused at the N-terminus of the fragment ...
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bioRxiv - Cell Biology 2019Quote: ... The solubilized fragment was purified by Talon-resin (Clontech/Takara) using the hexa-histidine-tag fused at the N-terminus of the fragment ...
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bioRxiv - Neuroscience 2019Quote: ... The lentivirus was purified by Lenti-XTM Concentrator (Clontech, 631231) and tittered by qPCR Lentivirus Titration Kit (Applied Biological Materials Inc. ...
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bioRxiv - Immunology 2020Quote: ... and purified over IMAC resin (HisTALON™ Superflow Cartridge, TAKARA), using a fast protein liquid chromatography system (ÄKTA FPLC Systems ...
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bioRxiv - Immunology 2020Quote: ... Fabs were purified by passage over Co-NTA agarose (Clontech) followed by gel filtration chromatography on Superdex 200 (GE Healthcare ...
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bioRxiv - Immunology 2020Quote: ... Fabs were purified by passage over Co-NTA agarose (Clontech) followed by gel filtration chromatography on Superdex 200 (GE Healthcare ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR products were pooled by template and purified (Nucleospin, Takara). RACE amplicons were end repaired ...