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Citations for Takara Bio :
351 - 400 of 780 citations for Family With Sequence Similarity 46 Member B FAM46B Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... the coding sequences of DivIVA and its T19A and T19E mutant alleles were PCR amplified using sequence-specific primers (Table 1) and cloned at BamHI-KpnI sites in pDsRed plasmid (Clontech Inc.) yielding pDsD4A and pDsD4AT19A ...
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bioRxiv - Plant Biology 2022Quote: ... Double-stranded DNAs corresponding to the guide RNA sequences were generated and inserted into AarI-digested pMpGE013 by ligation reaction using Mighty Mix (Takara Bio).
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Lateral organ diversification in plants mediated by the ALOG protein family of transcription factorsbioRxiv - Plant Biology 2019Quote: ... A PCR-amplified eGFP coding sequence was inserted in frame with the 5’ end of the MpTAW1 coding sequence by the In-Fusion cloning reaction (TaKaRa, Japan) to generate the proMpTAW1:eGFP-MpTAW1 plasmid ...
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Werner syndrome helicase is a selective vulnerability of microsatellite instability high tumor cellsbioRxiv - Cancer Biology 2019Quote: ... based on the WRN cDNA sequence NCBI NM_000553.5 followed by cloning into the parental pLVX vector (Clontech, Mountain View, CA, US). Codon optimization changes were introduced into WRN coding sequences to render the transgenes siRNA-resistant.
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bioRxiv - Zoology 2020Quote: ... the longer fragment containing the complete coding sequence of MYL2 was amplified from the longissimus dorsi muscle tissue with special paired-primers and Taq enzyme (Takara, Japan), and then the complete coding sequence of the target gene was amplified with special primers with restriction enzyme cutting sites and protecting bases ...
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Combinations of maternal-specific repressive epigenetic marks in the endosperm control seed dormancybioRxiv - Molecular Biology 2020Quote: ... and the REF6 coding sequence was inserted into the AatII and Eco32I sites using the In-Fusion® HD Cloning Kit (TaKaRa).
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bioRxiv - Immunology 2020Quote: ... the coding sequences of MRTFA and MRTFB were PCR amplified and subcloned into the pRetroX-Tight-Hygro vector (Takara Bio, 631034). Doxycycline inducible expression was achieved by pLVX-TetON-Advanced (Takara Bio ...
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bioRxiv - Developmental Biology 2021Quote: The MLP:H2B-Turquoise sequence (originally from BRE-MLP-H2B-Turquoise pGL3 basic, Briscoe lab, unpublished) was cloned using In-Fusion Cloning (Takara, 639650) between two chicken insulators at the BamHI site of plasmid pJC5-4 (Chung et al. ...
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ER exit sites in Drosophila display abundant ER-Golgi vesicles and pearled tubes but no megacarriersbioRxiv - Cell Biology 2021Quote: ... the coding sequence of each gene was amplified from whole larva cDNA using PrimeScript RT-PCR Kit (Takara, cat # RR014-A). The resulting products were recombined into pDONR221 and transferred to the pTWGA vector to finally produced the desired plasmids ...
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bioRxiv - Immunology 2021Quote: ... and the NES sequence of the HIV-1 rev protein into pT2ADW vector (Komatsu et al., 2018) by In-Fusion cloning (Takara Bio).
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bioRxiv - Immunology 2022Quote: ... The pINDUCER20-EYFP-mCITED1 lentiviral construct was produced by amplifying the full-length murine CITED1 coding sequence from pCDNA3-Flag-mCITED1 and subcloning it into pEYFP-C1 (Takara/Clontech) in-frame with the EYFP coding sequence ...
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bioRxiv - Molecular Biology 2022Quote: The G3BP1 cDNA sequence was amplified from a HeLa-JVM total cDNA library and concurrently inserted in-frame with a mCherry-coding sequence into a lentiviral vector (pCW)110 using the in-Fusion Cloning Kit (TaKaRa Bio). The HERC5 ...
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bioRxiv - Plant Biology 2022Quote: ... approximately 5,000 bp upstream sequences flanked the start codons of the MpSGFs were amplified from MpTak-1 genomic DNA using PrimeSTAR GXL polymerase (Takara Bio) and cloned into a linearized pENTR1A vector using In-fusion (Takara Bio) ...
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bioRxiv - Microbiology 2023Quote: ... a complete cDNA sequence of bovine hnRNPM (NM_001191223) was obtained from genomic DNA of MDBK cells using PrimeScript RT reagent Kit (TaKaRa, Shiga, Japan), followed by a PCR reaction using PrimeSTAR Max DNA Polymerase (TaKaRa ...
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bioRxiv - Microbiology 2023Quote: ... These identical sequences were generated via PCR with primers that contain a 5’ end identical to an adjacent segment and a 3’ end that anneals to the gene-of-interest sequence using a 2x hot-start PCR master mix (Takara, #R405A). The primers for PCR reactions were listed in Table 2.
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bioRxiv - Molecular Biology 2023Quote: ... Promoterless bicistronic vectors were generated by removing the SV40 promoter from the bicistronic vector pRUF and the pRUF vectors containing the putative IRES sequences by PCR amplification using divergent primers (Supplementary Table 3) and the CloneAmp™ HiFi PCR Premix (Takara) polymerase ...
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bioRxiv - Genomics 2023Quote: ... Primers were designed with around 15 bp of overlapping sequence to ensure proper circularization with the In-Fusion HD cloning plus kit (Takara #638909). Mach1 competent cells (Thermo Fisher Scientific #C862003 ...
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bioRxiv - Cell Biology 2023Quote: ... manuscript in preparation) The genetic sequence for H2B-mScarlet-I-DAAO and TOM20-mScarlet-I-DAAO was introduced by infusion cloning (Takara Bio) into a lentiviral backbone under control of a human EF-1α promoter and containing a puromycin selection cassette ...
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bioRxiv - Microbiology 2023Quote: ... the V5-tag sequence followed by a Stop codon was introduced upstream of the IRES in the pLVX-IRES-Neo vector (Clontech, Addgene), using annealed oligo-cloning ...
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bioRxiv - Synthetic Biology 2023Quote: ... DNA amplification of phage or bacterial genetic sequences were performed by PCR using the CloneAmp HiFi PCR premix® (Takara©). Primers used in this study are listed in Table S1 ...
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bioRxiv - Microbiology 2023Quote: ... This clone was digested with NsiI and BglII to remove the CMV promoter sequence and was combined with the pSV010(-) SV40 promoter fragment using the Infusion® HD Cloning kit (Clontech) such that the pSV010(- ...
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bioRxiv - Neuroscience 2023Quote: ... We then synthesized these sequences as G-blocks (Integrated DNA Technologies) and cloned them using Infusion kit (Takara biosciences catalog # 638948) into pSMART-HC-Kan (Lucigen ...
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bioRxiv - Plant Biology 2023Quote: ... the correct full-length sequences were determined for the laboratory strains using a SMARTer RACE 5’/3’ kit (Takara, Shiga, Japan). These sequences were aligned by ClustalW ...
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bioRxiv - Immunology 2023Quote: ... ORF9c or ORF10 accessory proteins coding sequences (codon-optimized for mammalian expression) were cloned into pLVX-EF1α-IRES-Puro Cloning and Expression Lentivector (Clontech, Takara, #631253) to generate pseudotyped lentiviral particles encoding each accessory protein of SARS-CoV-2 (Wuhan-Hu-1 isolate ...
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bioRxiv - Microbiology 2023Quote: ... linearized chimeric H3 HA sequence was gel purified using the NucleoSpin Gel and PCR Clean-up kit (Takara, Cat. No. 740609.5) and then purified using Ampure XP beads (Beckman Coulter ...
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bioRxiv - Biophysics 2023Quote: Broccoli RNA template sequences were ordered as dsDNA gBlock as above and inserted into a linearized pRZ plasmid using infusion cloning kit (Takara Bio) and transformed into Stellar cells following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The coding sequence of EPHX2 was amplified by PCR from liver cDNA (Human Multiple Tissue cDNA panels; Takara Bio, Shiga, Japan) using primers (5’-GTCGACATGACGCTGCGCGCGGCCGTCTTCG-3’ ...
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bioRxiv - Evolutionary Biology 2023Quote: Complete cDNA sequences of taste receptors were obtained using a SMARTer 5’/3’ kit for RACE PCR to obtain 5’ sequence information missing from reference transcriptome (Takara, #634858). Briefly ...
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bioRxiv - Evolutionary Biology 2024Quote: ... adhaerens Mint PDZ-1/PDZ-2/P1BM protein sequences (as described in the results section) were cloned into the mammalian bicistronic expression vector pIRES2-DsRed2 (Clontech, USA) to express recombinant proteins bearing C-terminal myc epitope tags (Figure 6K) ...
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bioRxiv - Biochemistry 2023Quote: ... The fusion between the core of the AGA1 promoter (-150 to 0) and the STL1 upstream activation sequence (-800 to -163) was generated by In-Fusion cloning (Takara Bio) into a plasmid containing the 24xPP7 stem loops ...
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bioRxiv - Cancer Biology 2024Quote: ... of the pDONR223 PCR product mixed with 243 fmol of each dsDNA insert sequences] and then mixed with 2µl of In-Fusion 5X HD Cloning Plus (Takara Bio #638909). The reaction was incubated for 15 minutes at 37°C and stored at −20°C ...
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bioRxiv - Cell Biology 2024Quote: ... the coding sequence of each gene was amplified from whole larva cDNA using PrimeScript RT-PCR Kit (Takara, cat # RR014-A). The amplified sequence was then purified through gel extraction (Magen HiPure Gel Pure DNA Mini kit ...
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bioRxiv - Biophysics 2024Quote: ... DNA sequence of EB3 were obtained from pETMz mGFP-EB3 (gift from Thomas Surrey lab) were cloned by infusion (Takara Bio) into the GCN4_v4 plasmids using the primer (EB3_LZ_for ...
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bioRxiv - Immunology 2019Quote: ... Transduction of PBMCs with LV-GXM-CAR constructs was performed using RetroNectin reagent (Takara Bio USA; cat. no. T100A/B) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: Amplicons were gel-purified and ligated into a pOPIN-B vector digested with HindIII and KpnI using In-Fusion technology (Clontech). The primer sequences in low case correspond to adaptors pairing with the linearized vector ...
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bioRxiv - Immunology 2021Quote: ... Following bulk BCR and TCR are prepared using SMARTer Mouse BCR IgG H/K/L Profiling Kit and SMARTer Mouse TCR a/b profiling kit separately (Takara). Based on the extracted mRNA amount of each sample ...
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bioRxiv - Microbiology 2021Quote: ... 5′-RACE cDNA was obtained from bulk-sorted splenic B cells of each mouse with the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (TaKaRa). The IgG PCRs were set up with Platinum Taq High-Fidelity DNA Polymerase (Life Technologies ...
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bioRxiv - Immunology 2021Quote: ... 5’ s-RACE cDNA was obtained from bulk-sorted B cells of each mouse with the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (TaKaRa). The IgG PCRs were set up with Platinum Taq High-Fidelity DNA Polymerase (Life Technologies ...
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bioRxiv - Cancer Biology 2019Quote: ... transfected cells were treated with 200 µg/mL hygromycin B (Nacalai Tesque, Kyoto, Japan) or 1 µg/mL puromycin (Clontech) for the selection of stably transfected cells.
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bioRxiv - Cancer Biology 2020Quote: ... Both CnAOEC and ISO-HAS-B were cultured in Endothelial Cell Growth Medium 2 Kit (Takara Bio, Inc. Kusatsu, Japan). All cells used were routinely tested for Mycoplasma using PCR and were submitted to ICLAS Monitoring Center (Kawasaki ...
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bioRxiv - Microbiology 2019Quote: ... 5’-RACE cDNA was obtained from bulk-sorted splenic B cells of each mouse with SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (TaKaRa). The immunoglobulin PCRs were set up with Platinum Taq High-Fidelity DNA Polymerase (Life Technologies ...
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bioRxiv - Genetics 2021Quote: ... This plasmid was further used to generate backbone structure containing fust-1 promoter and mRFP, to which cDNAs of FUST-1 isoforms (isoform a, isoform b, and ΔN) were inserted by In-Fusion (Takara). The mRFP fused FUST-1 cDNA plasmids were used to generate cDNA only plasmids for splicing reporter rescue by removing the mRFP sequences using In-Fusion (Takara) ...
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bioRxiv - Developmental Biology 2020Quote: ... and then further into the ClaI site of the avian replication-competent retrovirus vector RCASBP(B) (Li, Monckton, & Godbout, 2014) with the In-Fusion HD cloning kit (Clontech). The mutant construct that converts glutamine (Q ...
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bioRxiv - Microbiology 2020Quote: ... 5′-RACE cDNA was obtained from bulk-sorted B cells of each animal with SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (TaKaRa). The Ig PCRs were set up with Platinum Taq High-Fidelity DNA Polymerase (Thermo Fisher ...
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bioRxiv - Immunology 2020Quote: ... 10ng of RNA was used to prepare bulk TCR-seq libraries using the SMARTer Mouse TCR a/b Profiling Kit (Takara) according to instructions ...
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bioRxiv - Immunology 2023Quote: ... A cDNA library for TCR was prepared from RNA using a SMARTer Mouse TCR a/b Profiling Kit (Takara Bio) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... cDNA libraries were generated using SMARTer Human TCR a/b Profiling Kit v2 (Takara Bio USA, San Jose, California, USA). Briefly ...
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bioRxiv - Bioengineering 2023Quote: ... GA-MatryoshCaMP6s was amplified from pRSET B His-GA-MatryoshCaMP6s and subcloned into pDONR /Zeo via In-Fusion cloning (Takara Bio ...
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bioRxiv - Microbiology 2021Quote: ... The SARS-CoV-2 Spike sequence was cloned into the linearized pVSV-eGFP-dG by the In-Fusion cloning system (Takara Bio Inc.). The resulting pVSV-eGFP-deltaG_SARS-CoV-2 Spike vector was verified by sanger sequencing ...
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bioRxiv - Developmental Biology 2020Quote: ... Pegl-17::GFP1-10 constructs were generated by cloning egl-17 promotor and GFP1-10 sequences into pPD95.77 plasmids using In-Fusion Advantage PCR cloning kit (Clontech, cat. no. 639621). The plasmid was injected into SPC-1::GFP11×7 knock-in animals ...