Labshake search
Citations for Takara Bio :
3751 - 3800 of 5125 citations for Medium Kit without Serum and with CultureBoost since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2022Quote: Trophectoderm biopsies containing 5-10 cells from blastocyst-stage embryos (n=24) were processed for RNA-seq using a commercial kit (Takara Bio ...
-
bioRxiv - Molecular Biology 2022Quote: ... First-strand cDNA was synthesized from the total RNA using a PrimeScript™ RT Reagent Kit with gDNA Eraser (Takara) following the manufacturer’s protocols ...
-
bioRxiv - Immunology 2022Quote: ... – PU6-pAzpa was co-transfected with plasmids expressing full-lengths mutant 2B4 TCR and CD3 subunits using Xfect transfection kit (TaKaRa). For incorporating pAzpa into TCR sites ...
-
bioRxiv - Plant Biology 2022Quote: ... PCR fragments representing either the OsSOG1 promoter+GUS gene or OsSGL promoter+GUS gene were inserted into the AscI/AvrII site using an In-Fusion HD cloning kit (Takara) between attL1 and T35S in pE(L1-L2)hpt ...
-
bioRxiv - Microbiology 2022Quote: ... Then RNA was reverse-transcribed and amplified using One Step PrimeScriptTM III RT-qPCR Mix kit (Takara Bio, Kyoto, Japan). Primers and probes ...
-
bioRxiv - Immunology 2022Quote: ... First-strand cDNA was generated from 1 μg of RNA for each sample using the SMARTer RACE 5’/3’ Kit (Takara Bio ...
-
bioRxiv - Microbiology 2022Quote: ... The plasmids were extracted from all the colonies which grew on QDO/X/A agar plate using Easy Yeast Plasmid Isolation Kit (TaKaRa). PCR was carried out using the extracted plasmids as template ...
-
bioRxiv - Genetics 2022Quote: ... and 2 ng of total RNA was amplified with SMART-Seq v4 Ultra Low Input RNA kit (Clontech; version “091817”). Sequencing libraries were prepared with NEBnext Ultra DNA Library Prep Kit (New England Biolabs ...
-
bioRxiv - Cell Biology 2022Quote: ... reverse transcription and cDNA synthesis were performed using the SMART-Seq v4 Ultra Low RNA Kit for the Fluidigm C1 System (Takara) reagents ...
-
bioRxiv - Cell Biology 2022Quote: ... For each specimen 500 ng of total RNA was reversed transcribed into cDNA using PrimeScript RT reagent kit (TaKaRa RR037a) and oligo dT primers according to the manufacturer’s protocol ...
-
bioRxiv - Plant Biology 2022Quote: ... PtoATPE and PtoPSBB) were co-transformed into the Y1HGold yeast strain using the Matchmaker One-Hybrid Library Construction and Screening Kit (Clontech), respectively ...
-
bioRxiv - Microbiology 2022Quote: ... One μg of total RNA was used as input for SMARTer Stranded Total RNA Sample Prep Kit-HI Mammalian (Clontech). Sequencing was performed on the NextSeq500 instrument (Illumina ...
-
bioRxiv - Molecular Biology 2022Quote: ... Ribo-depleted sequencing libraries were prepared with the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (TaKaRa Biotech) following manufacturer instructions and sequenced with Genewiz/Azenta on an Illumina NovaSeq S4 machine using a 2 x 150 bp pair-end read configuration to a minimum depth of 30 million reads per sample ...
-
bioRxiv - Biophysics 2022Quote: ... 5’ and 3’-RACE-Ready cDNA templates were synthesized using a SMARTer® RACE 5’/3’ Kit (Takara Bio, USA) and subsequently used to amplify 5’ and 3’ end sequences of P ...
-
bioRxiv - Bioengineering 2022Quote: ... the gel-purified RNA was reverse transcribed into cDNA using a PrimeScript RT Reagent Kit with random primers (TAKARA, RR037B), followed by PCR with primers that can amplify transcripts across the splice junction ...
-
bioRxiv - Bioengineering 2022Quote: The cells were confirmed to be mycoplasma-negative every few months using a CycleavePCR Mycoplasma Detection Kit (CY232, Takara Bio).
-
bioRxiv - Cell Biology 2022Quote: ... and inserted between SpeI and NotI restriction sites (pFastBac1-hCAP-D3-[3C]-StrepII) by using restriction enzymes and a DNA ligation kit (TaKaRa). To create the hCAP-H2 construct with an N-terminal StrepII-tag (pFastBac-StrepII-[3C]-hCAP-H2) ...
-
bioRxiv - Developmental Biology 2022Quote: ... Single-cell cDNAs were prepared on Fluidigm C1 system by using SMARTer v4 Ultra Input Low RNA kit for the Fluidigm C1 System (Clontech). Only single-cell cDNAs confirmed by imaging data were performed quality check (QC ...
-
bioRxiv - Developmental Biology 2022Quote: ... the maximuM amount (10.5 µl) was used for library preparation using the SMART-Seq HT kit (Takara, Cat. No. 634437) followed by Nextera XT DNA Library Preparation Kit (Illumina ...
-
bioRxiv - Cell Biology 2022Quote: ... full length double-stranded cDNA was prepared from 1 ng of total RNA according to the manufacturer’s instructions for the SMART-Seq v4 Ultra Low Input RNA Kit® (Clontech). Quantity and quality of the amplified full length double-stranded cDNA were assessed using both Qubit (Invitrogen ...
-
bioRxiv - Neuroscience 2021Quote: ... RNA-Seq libraries were generated with the SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian (v1 or v2; Takara) according to the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2021Quote: ... DNAs of TOMM20-N-10-CLIPf and SNAPf-Clathrin-15 were directly PCR amplified from mammalian expression constructs and subcloned into lentiviral pHR-SFFV vector (BamHI/NotI) using In-Fusion HD Cloning kit (Clontech).
-
bioRxiv - Neuroscience 2020Quote: ... Total RNA was extracted from the isolated tissue and was subjected to reverse transcription (RT) with the use of a NucleoSpin RNA extraction kit (U955C, Takara) and PrimeScript RT reagent kit (RR037 ...
-
bioRxiv - Neuroscience 2020Quote: ... oligo(dT)-enriched RNA underwent 14 cycles of amplification using the SMARTer Ultra Low RNA Kit for Illumina Sequencing (Clontech). After cDNA fragmentation ...
-
bioRxiv - Neuroscience 2020Quote: ... cDNA libraries were produced using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara 634894, Lot 1709695A) according to the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2021Quote: 500 ng of the extracted total RNA were subjected to the first-strand cDNA synthesis with the primescript RT reagent kit (Takara). Quantitative real-time PCR was performed with Thunderbird SYBR qPCR mix (Toyobo ...
-
bioRxiv - Plant Biology 2021Quote: The Pro197Ser-mutated ALS genes and the native form of MvALS1 gene were inserted into the pCAMBIA1390 vector under the cauliflower mosaic virus 35S promoter using the In-Fusion DH Cloning Kit (TaKaRa), as described previously (Iwakami et al. ...
-
bioRxiv - Plant Biology 2021Quote: ... after-ripened Col and ref6c seeds collected at different imbibition time points were subjected for total RNA extraction using Minibest plant RNA extraction kit (Takara). Three independent biological replicates were performed ...
-
bioRxiv - Plant Biology 2021Quote: ... The amplicons from SlACS2-prom1k-pPLV-F/R were cloned into pPLV451 using an In-Fusion HD Cloning kit (Clontech), to construct pACS2-GFPx3 and pACS2mut-GFPx3 ...
-
bioRxiv - Microbiology 2020Quote: ... gene was then PCR amplified and purified then cloned into plasmid pLC29144 using In-Fusion HD Cloning plus kit (Takara). All PCR products and linearized vector were purified (Macherey and Nagel ...
-
bioRxiv - Microbiology 2020Quote: ... overhangs upstream and downstream of the gene of interest were amplified (primers sequences available in Table S1) and purified then cloned into linearized pCM433 using In-Fusion HD Cloning plus kit (Takara), primer design was done using primer design tool (Takara) ...
-
bioRxiv - Developmental Biology 2021Quote: ... Libraries were prepared from 50 ng of total RNA/sample as input using Ribogone and a SMARTer Universal Low Input RNA Kit (Clontech). For inDrops ...
-
bioRxiv - Molecular Biology 2020Quote: Primary RT-PCR and secondary PCR was performed using the Primescript III 1-step RT-PCR kit and EmeraldAmp GT PCR Mix (Takara; detailed protocol provided in Supplementary File 1) ...
-
bioRxiv - Genomics 2019Quote: ... The IsoSeq libraries were created starting from 1µg of total RNA per sample and full-length cDNA was then generated using the SMARTer PCR cDNA synthesis kit (Clontech) following PacBio recommendations set out in the IsoSeq method (pacb.com/wp-content/uploads/Procedure-Checklist-Iso-Seq-Template-Preparation-for-Sequel-Systems.pdf) ...
-
bioRxiv - Molecular Biology 2019Quote: ... cerevisiae CLN2 was appended to the C-terminal codon of the HIS3 gene using the In-Fusion Cloning Kit (Clontech) as previously described (2) ...
-
bioRxiv - Genomics 2021Quote: ... These nanowells were selected for subsequent targeted deposition of 50□nl/nanowell RT-PCR reaction mix from the SMARTer ICELL8 3’ DE Reagent Kit (Takara) using the MSND ...
-
bioRxiv - Neuroscience 2021Quote: ... Ten ng of total RNA were amplified and converted into cDNA using a SMART-Seq v4 Ultra Low Input RNA Kit (Clontech). Next ...
-
bioRxiv - Neuroscience 2020Quote: The cDNA libraries for used for sequencing of 4 total RNA samples were synthesized using a SMART-Seq Ultra Low Input RNA kit (Takara) in the OHSU Massively Parallel Sequencing Shared Resource Core Facility ...
-
bioRxiv - Microbiology 2021Quote: ... PCR amplifications were conducted in 50-μL total volumes containing 1 μL of template DNA using an ExTaq kit (Takara). The cycling protocol was as follows ...
-
bioRxiv - Plant Biology 2021Quote: Tagmented DNA was amplified directly from the beads via 6 cycles of PCR amplification using the PrimeSTAR GXL Polymerase kit (Takara) and dual indexed adapters (Illumina ...
-
bioRxiv - Neuroscience 2021Quote: RNA from 30,000 cultured adult NSCs or whole DGs were isolated with the Clontech Nucleospin RNA XS Plus isolation kit (Takara 740990.10) per manufacturer protocol ...
-
bioRxiv - Plant Biology 2021Quote: ... which was used to synthesize first-strand cDNA using the SuperScript II first-strand cDNA synthesis Kit™ (Takara, Japan). The Arabidopsis Actin2 gene was used as the internal reference gene.
-
bioRxiv - Microbiology 2020Quote: The DNA samples were prepared for Illumina sequencing using the SMARTer® ThruPLEX® DNA-Seq kit (Takara Bio, USA) according to manufacturer protocol ...
-
bioRxiv - Immunology 2020Quote: ... Libraries were constructed from total RNA with the ‘SMARTer Stranded Total RNA-Seq Kit v2 – Pico Input Mammalian’ (Takara/Clontech) according to manufacturer’s recommendations ...
-
bioRxiv - Immunology 2020Quote: ... Libraries were constructed from total RNA with the ‘SMARTer Stranded Total RNA-Seq Kit v2 – Pico Input Mammalian’ (Takara/Clontech) according to manufacturer’s recommendations ...
-
bioRxiv - Neuroscience 2020Quote: ... And then the DNA fragment encoding 4R1N tau were inserted into the linearized pHR-mCh-Cry2Olig backbone using In-Fusion Cloning Kit (Takara). The resulting constructs were fully sequenced to confirm the absence of unwanted substitutions.
-
Physiological activation of the nephron central command drives endogenous kidney tissue regenerationbioRxiv - Physiology 2021Quote: ... Libraries were simultaneously prepared using Takara’s SMARTer Stranded Total-RNA Pico v2 library preparation kit following manufacturer’s protocol (Takara cat#634412). Prepared libraries were sequenced on Illumina Nextseq500 at 2×75cycles.
-
bioRxiv - Microbiology 2021Quote: ... 72 °C 20 seconds for 35 cycles followed by subcloning into the eukaryotic expression vector pCAGGs using the In-Fusion HD cloning kit (Takara). All plasmids were midiprepped using the Nucleobond Xtra midi kit (Macherey-Nagel) ...
-
Q-SNARE Syntaxin 7 confers actin-dependent rapidly replenishing synaptic vesicles upon high activitybioRxiv - Neuroscience 2020Quote: ... they were cloned into a SmaI site located downstream of the TRE sequence in pLenti6PW–TRE using an In–Fusion Cloning Kit (Clontech)(Egashira et al ...
-
bioRxiv - Biophysics 2021Quote: ... The resulting DNA fragments were gel-extracted and cloned individually into the pBADC3 plasmid using InFusion EcoDry cloning kit (TaKaRa). All genes were fused with a C-terminal StrepII-tag for affinity purification.