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Citations for Takara Bio :
3701 - 3750 of 5557 citations for Human E3 Ubiquitin Protein Ligase SMURF2 SMURF2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The PCR product was gel purified using the Nucleospin Gel and PCR Clean-Up Kit (TaKaRa Bio Inc.) and cloned in between the XhoI and SalI sites of psiCHECK-2.2 using InFusion cloning (TaKaRa Bio Inc.) ...
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bioRxiv - Developmental Biology 2023Quote: ... Purified total RNA was reverse transcribed into cDNA by PrimeScript II 1st strand cDNA Synthesis Kit (Takara, 6210A) or SMARTer™ RACE cDNA Amplification Kit (Clontech) ...
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bioRxiv - Developmental Biology 2023Quote: ... Polyadenylated RNA-seq libraries were prepared by using SMART-Seq v4 PLUS Kit (Clontech, Mountain View, CA, USA). Indexed RNA-seq libraries were sequenced using Illumina HiSeqX sequencer (paired end ...
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bioRxiv - Synthetic Biology 2023Quote: ... Specific primers were designed for this purpose and PCR was done using the Advantage 2 Polymerase kit (Clontech). Complementary 60 bp-ends to each target sequence on all Mycoplasmas species genome used in this study ...
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bioRxiv - Plant Biology 2023Quote: ... The double-strand oligonucleotides containing the target sequences were synthesized and cloned into pMpGE_En01 or pMpGE_En03 using In-Fusion HD cloning kit (TaKaRa) and BsaI restriction sites in pMpGE_En03 ...
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bioRxiv - Plant Biology 2023Quote: ... cDNA was synthesized using 1 µg of RNA following manufacturers’ instruction (1st strand cDNA synthesis kit, Takara; 6110A). RT-PCR was performed using SapphireAmp® Fast PCR Master Mix (Takara ...
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bioRxiv - Plant Biology 2023Quote: ... SRF mutants were prepared using PrimeSTAR Mutagenesis Basal Kit according to the manufacturer’s instructions (TaKaRa Bio, Shiga, Japan), and the mutations were confirmed by sequencing ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Complete mtDNA was amplified in several overlapping fragments using the Long and Accurate (LA) PCR kit from TAKARA.
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bioRxiv - Cell Biology 2023Quote: ... synthesized double strand DNA of BrUSLEE-SKL was cloned to pT1O using In-Fusion HD Cloning Kit (Takara). DNA fragments of mCherry-SKL ...
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bioRxiv - Molecular Biology 2023Quote: ... The amplified fragments and vector pBFv-UAS3 were fused using the In-Fusion® HD Cloning Kit (TaKaRa). The oligonucleotide sequences used are shown in Supplementary Table 2 ...
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bioRxiv - Plant Biology 2023Quote: ... and cloned into the XbaI digestion site of pMpGWB307 vectors using In-Fusion HD Cloning Kit (Takara Bio) to produce pMpGWB307-MpJINp ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR products were cut from agarose gels and purified using the Nucleospin gel purification kit (Takara Bio, 240609). Plasmids were assembled from linear fragments using In-Fusion HD (Takara Bio ...
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bioRxiv - Physiology 2023Quote: Total RNA was extracted from tissues using the RNAiso Plus kit and treated with DNase I (TaKaRa, China). The purified RNA was used for first-strand cDNA synthesis ...
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bioRxiv - Microbiology 2023Quote: ... Purified RNA was quantified and used for cDNA preparation using the PrimeScript 1st strand cDNA Synthesis Kit (Takara) with 1 μg of extracted RNA ...
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bioRxiv - Plant Biology 2023Quote: ... after which first-strand cDNA was synthesized from total RNA using a cDNA reverse transcription kit (TaKaRa, Japan). Full-length CDS sequences of the three candidate UGT genes were obtained from third-generation transcriptome sequencing data (Chen et al ...
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bioRxiv - Plant Biology 2023Quote: ... RNA (0.5 μg) was reverse transcribed by the PrimeScript RT reagent kit with genomic DNA Eraser (Takara, USA) using random and oligo-dT primer mix in a 10 μL reaction ...
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bioRxiv - Immunology 2023Quote: Reverse transcription and cDNA amplification were performed with the SMART-Seq v4 Ultra Low Input RNA Kit (Takara). Barcoded Illumina-compatible libraries were generated from 5 to 10Lng of DNA of each sample ...
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bioRxiv - Genomics 2023Quote: ... Libraries were prepared using the SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian (Takara Bio USA, Inc.), which incorporates both RiboGone and SMART (Switching Mechanism At 5’ end of RNA Template ...
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bioRxiv - Genomics 2023Quote: ... Illumina sequencing libraries were prepared from ChIP-enriched DNA using ThruPLEX DNA-seq library preparation kit (Takara Bio) with up to 10ng of input DNA and 8-15 PCR cycles ...
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bioRxiv - Genetics 2023Quote: ... The RNA was reverse-transcribed into cDNA by using a Prime ScriptTM RT Reagent Kit (TaKaRa, Kyoto, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR products were cut from agarose gels and purified using the Nucleospin gel purification kit (Takara Bio, 240609). Final plasmids were constructed using In-Fusion HD (Takara Bio ...
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bioRxiv - Cancer Biology 2022Quote: ... DNA fragmentation was performed using In situ Apoptosis Detection Kit according to the manufacturer’s indications (Takara Bio Inc.). For immunostaining ...
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bioRxiv - Cell Biology 2023Quote: ... and N-SREBP2 (1440 bps) were amplified using high-fidelity PCR (Advantage-HF 2 PCR kit, #639123; Clontech) from human pcDNA3.1-2xFLAG-SREBP1a (#26801 ...
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bioRxiv - Immunology 2023Quote: ... Total RNA was normalized prior to oligo-dT capture and cDNA synthesis with SMART-seq v4 kit (Takara). The resulting cDNA was quantitated using a Qubit 3.0 fluorometer ...
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bioRxiv - Immunology 2023Quote: ... linearized BF520 sequence was gel purified using NucleoSpin Gel and PCR Clean-up kit (Takara, Cat. No. 740609.5) and then purified using Ampure XP beads (Beckman Coulter ...
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bioRxiv - Cell Biology 2023Quote: ... and a PrimeScript RTreagent kit with gDNA Eraser and SYBR Premix Ex Taq II (TliRNase H Plus) (TaKaRa) and an ABI7500 qPCR instrument (Life Technologies) ...
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bioRxiv - Microbiology 2023Quote: ... The PCR product was cloned into SalI-PstI-digested pKG120 by In-Fusion HD Cloning Kit (Takara bio). The resulting plasmid ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was prepared using the SMARTer procedure through the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech) for Illumina Sequencing ...
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bioRxiv - Molecular Biology 2023Quote: Library preparation: cDNA was synthesized with the SMARTer Universal Low Input RNA Kit protocol (Clontech, cat. no. 634938) using the standard RiboGone–Mammalian Kit protocol.
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bioRxiv - Developmental Biology 2023Quote: ... 18-20 μg of indicated plasmids were transfected in HEK293T cells using the CalPhos Mammalian Transfection Kit (Takara). After 48h ...
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bioRxiv - Developmental Biology 2023Quote: ... and subcloned into the EcoRI site of pT2A-BItight-TRE by In-Fusion® HD cloning kit (TaKaRa). pT2A-BItight-Lifeact-mCherry-TRE ...
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bioRxiv - Plant Biology 2023Quote: ... and terminator was assembled into the backbone PCB1532 via In-Fusion cloning (In-Fusion cloning kit, Clontech Laboratories).
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bioRxiv - Plant Biology 2023Quote: ... 4 μg of total RNA was used for cDNA synthesis with PrimeScript 1st strand cDNA Synthesis Kit (Takara), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... First strand cDNA synthesis was performed on 200ng RNA using the SMARTer PCR cDNA Synthesis Kit (Clontech, UK) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... Libraries were prepared using the SMARTer Stranded Total RNA-Seq kit v2 - Pico Input Mammalian (Clontech, TaKaRa Bio). Unfragmented RNA was reverse transcribed with adapter-linked random hexamers and the first strand cDNA was then converted into double-stranded dual-indexed DNA libraries ...
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bioRxiv - Microbiology 2023Quote: ... 5’RACE was performed with SMARTer RACE 5’/3’ Kit (Takara Bio USA, Inc. San Jose, CA USA) according to the manufacturer’s directions.
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bioRxiv - Cell Biology 2023Quote: ... Sequencing libraries from treated RNA were created using the SMARTer Stranded Total RNA HI Mammalian kit (Takara 634873) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: All libraries were prepared using the SMARTer Stranded Total RNaseq Kit v2 – Pico Input Mammalian Components (634419, Takara) according to manufacturer’s protocols and barcoded (634452 ...
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bioRxiv - Genomics 2023Quote: ... Libraries were prepared using the SMARTer Stranded Total RNA-Seq kit v2 - Pico Input Mammalian (Clontech, TaKaRa Bio). Unfragmented RNA was reverse transcribed with adapter-linked random hexamers and the first strand cDNA was then converted into double-stranded dual-indexed DNA libraries ...
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bioRxiv - Immunology 2023Quote: ... Genomic DNA was isolated from tail biopsies using the NucleoSpin Tissue kit (TaKaRa Bio USA, San Jose, CA) following manufacture protocol ...
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bioRxiv - Microbiology 2023Quote: ... Reverse transcription was performed to make cDNA using PrimeScript RT reagent kit with gDNA Eraser (TaKaRa, Kyoto, Japan). Sequences encoding the C-terminal portion (amino acid 45 to 131 ...
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bioRxiv - Immunology 2023Quote: ... Total RNA was normalized prior to oligo-dT capture and cDNA synthesis with SMART-seq v4 kit (Takara). The resulting cDNA was quantitated using a Qubit 3.0 fluorometer ...
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bioRxiv - Molecular Biology 2023Quote: ... The 5’ and 3’ ends of the cDNA were amplified using the SMARTer RACE cDNA Amplification Kit (Clontech) according to the manufacturer’s guidelines ...
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bioRxiv - Bioengineering 2023Quote: ... Purified insert DNA was cloned into the linearized modRNAc1 plasmid using the In-Fusion Cloning Kit (Takara Bio). The DNA template for modRNA synthesis was PCR amplified from the successfully cloned modRNAc1 plasmid followed by PCR purification using DNA Clean & Concentrator-5 (Zymo Research) ...
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bioRxiv - Biochemistry 2023Quote: ... Genomic DNA from WT and Strt1−/− cells was extracted with a NucleoSpin Tissue kit (Takara Bio, Shiga, Japan) and subjected to PCR using specific primers (Oligonucleotide sets 3 ...
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bioRxiv - Bioengineering 2023Quote: ... The synthesized mRNA was subsequently purified using a NucleoSpin® RNA Clean-Up Kit (Takara Bio Inc., Japan). The concentration of the purified mRNA was quantified using a Nanodrop One (Thermo Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Dox-inducible vectors were generated according to a publicly available protocol54 using In-Fusion HD Cloning Kit (Clontech). Vectors were amplified in Stellar Competent Cells (Clontech ...
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bioRxiv - Biophysics 2023Quote: ... The first-strand cDNA was reverse transcribed with PrimeScript™ RT Reagent Kit with gDNA Eraser (TaKaRa, Japan). Quantitative real-time PCR was run on a Roche LightCycler® 480 II real-time PCR detection system ...
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bioRxiv - Plant Biology 2023Quote: ... was performed to determine the expression level of transcripts utilizing the SYBR Green PCR master mix kit (Takara). The unique region of transcripts was selected for designing qRT-PCR primers and mentioned in Table S3 ...
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bioRxiv - Genetics 2024Quote: ... These three fragments were cloned into EcoRI-digested pBluescript-II vector using In-Fusion HD cloning kit (Takara). gRNA plasmids were constructed by cloning of annealed oligo DNA (Ti902/903 and Ti904/905 ...