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Citations for Takara Bio :
3551 - 3600 of 5356 citations for Pig Glucagon Like Peptide 2 GLP 2 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Purified RNA was quantified and used for cDNA preparation using the PrimeScript 1st strand cDNA Synthesis Kit (Takara) with 1 μg of extracted RNA ...
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bioRxiv - Physiology 2023Quote: Total RNA was extracted from tissues using the RNAiso Plus kit and treated with DNase I (TaKaRa, China). The purified RNA was used for first-strand cDNA synthesis ...
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bioRxiv - Plant Biology 2023Quote: ... after which first-strand cDNA was synthesized from total RNA using a cDNA reverse transcription kit (TaKaRa, Japan). Full-length CDS sequences of the three candidate UGT genes were obtained from third-generation transcriptome sequencing data (Chen et al ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR products were cut from agarose gels and purified using the Nucleospin gel purification kit (Takara Bio, 240609). Plasmids were assembled from linear fragments using In-Fusion HD (Takara Bio ...
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bioRxiv - Plant Biology 2023Quote: ... and cloned into the XbaI digestion site of pMpGWB307 vectors using In-Fusion HD Cloning Kit (Takara Bio) to produce pMpGWB307-MpJINp ...
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bioRxiv - Plant Biology 2023Quote: ... The double-strand oligonucleotides containing the target sequences were synthesized and cloned into pMpGE_En01 or pMpGE_En03 using In-Fusion HD cloning kit (TaKaRa) and BsaI restriction sites in pMpGE_En03 ...
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bioRxiv - Plant Biology 2023Quote: ... cDNA was synthesized using 1 µg of RNA following manufacturers’ instruction (1st strand cDNA synthesis kit, Takara; 6110A). RT-PCR was performed using SapphireAmp® Fast PCR Master Mix (Takara ...
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bioRxiv - Plant Biology 2023Quote: ... RNA (0.5 μg) was reverse transcribed by the PrimeScript RT reagent kit with genomic DNA Eraser (Takara, USA) using random and oligo-dT primer mix in a 10 μL reaction ...
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bioRxiv - Immunology 2023Quote: Reverse transcription and cDNA amplification were performed with the SMART-Seq v4 Ultra Low Input RNA Kit (Takara). Barcoded Illumina-compatible libraries were generated from 5 to 10Lng of DNA of each sample ...
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bioRxiv - Genomics 2023Quote: ... Libraries were prepared using the SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian (Takara Bio USA, Inc.), which incorporates both RiboGone and SMART (Switching Mechanism At 5’ end of RNA Template ...
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bioRxiv - Genomics 2023Quote: ... Illumina sequencing libraries were prepared from ChIP-enriched DNA using ThruPLEX DNA-seq library preparation kit (Takara Bio) with up to 10ng of input DNA and 8-15 PCR cycles ...
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bioRxiv - Genetics 2023Quote: ... The RNA was reverse-transcribed into cDNA by using a Prime ScriptTM RT Reagent Kit (TaKaRa, Kyoto, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR products were cut from agarose gels and purified using the Nucleospin gel purification kit (Takara Bio, 240609). Final plasmids were constructed using In-Fusion HD (Takara Bio ...
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bioRxiv - Cancer Biology 2022Quote: ... DNA fragmentation was performed using In situ Apoptosis Detection Kit according to the manufacturer’s indications (Takara Bio Inc.). For immunostaining ...
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bioRxiv - Immunology 2023Quote: ... Total RNA was normalized prior to oligo-dT capture and cDNA synthesis with SMART-seq v4 kit (Takara). The resulting cDNA was quantitated using a Qubit 3.0 fluorometer ...
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bioRxiv - Immunology 2023Quote: ... linearized BF520 sequence was gel purified using NucleoSpin Gel and PCR Clean-up kit (Takara, Cat. No. 740609.5) and then purified using Ampure XP beads (Beckman Coulter ...
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bioRxiv - Cell Biology 2023Quote: ... and a PrimeScript RTreagent kit with gDNA Eraser and SYBR Premix Ex Taq II (TliRNase H Plus) (TaKaRa) and an ABI7500 qPCR instrument (Life Technologies) ...
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bioRxiv - Microbiology 2023Quote: ... The PCR product was cloned into SalI-PstI-digested pKG120 by In-Fusion HD Cloning Kit (Takara bio). The resulting plasmid ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was prepared using the SMARTer procedure through the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech) for Illumina Sequencing ...
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bioRxiv - Plant Biology 2023Quote: ... and terminator was assembled into the backbone PCB1532 via In-Fusion cloning (In-Fusion cloning kit, Clontech Laboratories).
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bioRxiv - Neuroscience 2023Quote: ... First strand cDNA synthesis was performed on 200ng RNA using the SMARTer PCR cDNA Synthesis Kit (Clontech, UK) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... 4 μg of total RNA was used for cDNA synthesis with PrimeScript 1st strand cDNA Synthesis Kit (Takara), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Sequencing libraries from treated RNA were created using the SMARTer Stranded Total RNA HI Mammalian kit (Takara 634873) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... was performed to determine the expression level of transcripts utilizing the SYBR Green PCR master mix kit (Takara). The unique region of transcripts was selected for designing qRT-PCR primers and mentioned in Table S3 ...
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bioRxiv - Plant Biology 2024Quote: ... amplified promoter region fragments are inserted with KpnI and XhoI site in pAT006-Fluc-iG5_Rluc-iFAD2_F vector using In-Fusion HD Cloning Kit (Takara). Amplified BOT6 fragment was inserted with pGWB402 using pENTR™ Directional TOPO® Cloning Kits (Invitrogen).
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bioRxiv - Developmental Biology 2024Quote: Single-cell cDNA synthesis was performed using SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara) according to the manufacturer’s protocol with some modifications as previously described17 ...
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bioRxiv - Synthetic Biology 2024Quote: ... One µg of total RNA was processed using PrimeScript RT reagent Kit with gDNA Eraser (Takara, Beijing, China). Amplified products were sequenced and verified after cloning into a pJET1.2-T vector through CloneJET PCR Cloning Kit (Thermo Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... The pmEOS2-C1-ERGIC53 plasmid was constructed using using the In-Fusion® Snap Assembly Cloning Kit (Takara) and transformed into OmniMAX competent cells ...
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bioRxiv - Microbiology 2023Quote: ... Reverse transcription was performed to make cDNA using PrimeScript RT reagent kit with gDNA Eraser (TaKaRa, Kyoto, Japan). Sequences encoding the C-terminal portion (amino acid 45 to 131 ...
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bioRxiv - Immunology 2023Quote: ... Total RNA was normalized prior to oligo-dT capture and cDNA synthesis with SMART-seq v4 kit (Takara). The resulting cDNA was quantitated using a Qubit 3.0 fluorometer ...
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bioRxiv - Molecular Biology 2023Quote: ... The 5’ and 3’ ends of the cDNA were amplified using the SMARTer RACE cDNA Amplification Kit (Clontech) according to the manufacturer’s guidelines ...
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bioRxiv - Biochemistry 2023Quote: ... Genomic DNA from WT and Strt1−/− cells was extracted with a NucleoSpin Tissue kit (Takara Bio, Shiga, Japan) and subjected to PCR using specific primers (Oligonucleotide sets 3 ...
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bioRxiv - Biophysics 2023Quote: ... The first-strand cDNA was reverse transcribed with PrimeScript™ RT Reagent Kit with gDNA Eraser (TaKaRa, Japan). Quantitative real-time PCR was run on a Roche LightCycler® 480 II real-time PCR detection system ...
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bioRxiv - Bioengineering 2023Quote: ... The synthesized mRNA was subsequently purified using a NucleoSpin® RNA Clean-Up Kit (Takara Bio Inc., Japan). The concentration of the purified mRNA was quantified using a Nanodrop One (Thermo Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... Purified insert DNA was cloned into the linearized modRNAc1 plasmid using the In-Fusion Cloning Kit (Takara Bio). The DNA template for modRNA synthesis was PCR amplified from the successfully cloned modRNAc1 plasmid followed by PCR purification using DNA Clean & Concentrator-5 (Zymo Research) ...
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bioRxiv - Genomics 2023Quote: ... Libraries were prepared using the SMARTer Stranded Total RNA-Seq kit v2 - Pico Input Mammalian (Clontech, TaKaRa Bio). Unfragmented RNA was reverse transcribed with adapter-linked random hexamers and the first strand cDNA was then converted into double-stranded dual-indexed DNA libraries ...
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bioRxiv - Developmental Biology 2023Quote: ... 18-20 μg of indicated plasmids were transfected in HEK293T cells using the CalPhos Mammalian Transfection Kit (Takara). After 48h ...
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bioRxiv - Microbiology 2023Quote: ... 5’RACE was performed with SMARTer RACE 5’/3’ Kit (Takara Bio USA, Inc. San Jose, CA USA) according to the manufacturer’s directions.
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bioRxiv - Cancer Biology 2023Quote: ... Dox-inducible vectors were generated according to a publicly available protocol54 using In-Fusion HD Cloning Kit (Clontech). Vectors were amplified in Stellar Competent Cells (Clontech ...
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bioRxiv - Plant Biology 2024Quote: ... cDNA was prepared from 1.5 μg of total RNA using the PrimeScript 1st strand cDNA Synthesis Kit (Takara). PCR reactions were performed with 1 μl of a 1:4 dilution of cDNA in ddH2O ...
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bioRxiv - Plant Biology 2024Quote: ... The resulting samples were reverse transcribed into first-strand cDNA using a PrimeScript RT Reagent Kit (Takara Bio). Quantitative PCR (qPCR ...
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bioRxiv - Plant Biology 2024Quote: ... We then performed first-strand synthesis using the SMARTer Stranded Total RNA Kit V2- Pico Input Mammalian (Takara). Following purification with RNAclean XP (Beckman Coulter) ...
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bioRxiv - Zoology 2024Quote: ... One µg of total RNA was processed using PrimeScript RT reagent Kit with gDNA Eraser (Takara, Beijing, China). The PCR procedure was set as follows ...
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bioRxiv - Immunology 2024Quote: ... Viral titer (p24 core antigen content) was determined initially using a LentiX GoStix Plus titration kit (Takara #631280) and subsequently by HIV p24 AlphaLISA detection kit (PerkinElmer # AL291C) ...
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bioRxiv - Cell Biology 2024Quote: ... One microgram of total RNA was subjected to first strand cDNA synthesis using PrimeScript RT Reagent kit (Takara). The target cDNA was amplified using TB Green Premix Ex Taq II (Takara ...
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bioRxiv - Cell Biology 2024Quote: ... One microgram of total RNA was subjected to first strand cDNA synthesis using PrimeScript RT Reagent kit (Takara). The target cDNA was amplified using TB Green Premix Ex Taq II (Takara ...
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bioRxiv - Genomics 2024Quote: Sequencing libraries were prepared from isolated RNA using two different kits: Takara SMART-Seq v4 3′ DE (Takara) and Lexogen QuantSeq 3′ (Lexogen ...
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bioRxiv - Genetics 2024Quote: ... Ligation of the various components was undertaken using an In-Fusion cloning kit (Takara Bio-Europe, Paris, France). The ligation reaction was transformed into Stellar competent cells as per manufacturer protocols ...
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bioRxiv - Genetics 2024Quote: ... Complementary DNA (cDNA) was synthesized from total RNA using a PrimeScript RT reagent Kit (Cat. no. RR047B, TaKaRa) and was used as a template to perform qPCR in TB green-based qPCR instrument (CFX-96 ...
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bioRxiv - Neuroscience 2024Quote: ... and 3 μg of RNA was reverse transcribed using PrimeScript™ RT reagent kit (Takara, San Jose, CA), according to manufacturer’s instruction ...