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Citations for Takara Bio :
3451 - 3500 of 4905 citations for Thyroxine ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... the NS3 fragments with FLAG-tag and NS3 cDNA vector were ligated together at an approximate molar ratio of 1:3 using TaKaRa DNA Ligation Kit LONG (TAKARA) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2019Quote: ... The IL-1β and components of RISC mRNA expression levels of the NA cells were quantified with the SYBR Green qPCR kit (Takara) by following the manufacturer’s instruction using gene-specific primers (S2 Table) ...
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bioRxiv - Immunology 2019Quote: ... JUN-P2A was then subcloned into the XhoI site of MSGV CAR vectors using the In-Fusion HD cloning kit (Takara) upstream of the CAR leader sequence to create JUN-P2A-CAR retroviral vectors ...
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bioRxiv - Developmental Biology 2019Quote: ... 5’ and 3’ RACE was carried out according to the manufacturer’s protocol of SMART RACE cDNA Amplification kit (Clontech, Takara, Japan). The sequences of primers for RACE are as follows ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and a modified vector with an N-terminal AviTag for biotinylation and C-terminal His6-tag (p28BIOH-LIC) using a ligation-independent InFusion cloning kit (ClonTech) and verified by DNA sequencing ...
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bioRxiv - Microbiology 2019Quote: ... These PCR products were cloned into pCA24N with a C-terminal GFP tag using the In-Fusion HD enzyme kit (Takara). Clones were selected on TSA plates with 50µg/ml chloramphenicol ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The PCR products were cloned into pBAD28 vector (ATCC® 87400™) by using In-Fusion HD Cloning kit (Takara) by using primers 7 and 8 to amplify pBAD28 vector (Table S2 ...
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bioRxiv - Molecular Biology 2019Quote: ... cDNA was synthesized from 1.0 μg of each RNA sample using PrimeScript RT Kit with gDNA Eraser (TaKaRa, Dalian, China).
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bioRxiv - Immunology 2019Quote: ... was added to each sample and first strand full length cDNA was generated with a modified protocol of the SMARTseq v4 Ultra Low Input RNA Kit (Takara Clontech) using poly dT primers and a template switching oligo ...
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bioRxiv - Molecular Biology 2019Quote: ... The RNA was subjected to reverse transcription (RT) for 15 min at 37°C with the use of a PrimeScript RT Reagent Kit with gDNA Eraser (Takara), after which the reaction was terminated by incubation at 85°C for 5 s ...
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bioRxiv - Cancer Biology 2019Quote: ... containing a c-terminal Influenza Hemagglutinin (HA) reporter tag was cloned into the backbone pLX317-empty using the In-Fusion cloning kit (Clontech). The backbone was cut with BamHI and EcoRI.
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Screening and identification of MicroRNAs expressed in perirenal adipose tissue during rabbit growthbioRxiv - Developmental Biology 2019Quote: ... Six DE miRNAs were reverse transcribed into cDNA using Mir-X™ miRNA First-Strand Synthesis Kits (Takara, Dalian, China) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... 3μg of total RNA was used for reverse transcription with the Prime Script First Strand cDNA Synthesis Kit (Takara, D6110A). Quantitative real-time PCR (qPCR ...
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bioRxiv - Developmental Biology 2019Quote: ... 250-2000 pg of total RNA was loaded as a template for cDNA synthesis using the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech) with template switching technology ...
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bioRxiv - Biochemistry 2019Quote: ... The ERE sequences of URAT1/SLC22A12 promoter region was amplified to get mutants using PrimeSTAR® Mutagenesis Basal Kit (Takara) with the following primers ...
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bioRxiv - Genomics 2020Quote: ... PCRs were performed on board using 25 ng of DNA from each coral sample with the Advantage 2 kit (Takara Clontech) and a final primer concentration of 0.5 μM in a final reaction volume of 50 μl ...
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bioRxiv - Pathology 2021Quote: ... Viral titering was performed using 15 μL of unconcentrated virus or 1.5 μl of concentrated virus with the Lenti-X qRT-PCR Titration Kit (Clontech, 632165). Results were read on an ABI QuantStudio 6 RT-PCR machine.
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bioRxiv - Systems Biology 2020Quote: We extracted RNA from fixed cells after barcode RNA FISH and sorting using the NucleoSpin total RNA FFPE XS kit (Takara). We performed cell lysis and reverse cross-linking at 50°C for 90 minutes and otherwise followed the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... 5′UTR of test genes and EGFP were inserted into pCAG-FLAG-N1 plasmid 48 using In-Fusion HD Cloning Kit (TAKARA).
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bioRxiv - Developmental Biology 2021Quote: ... Reverse transcription was performed using 2.5 µl of the RT MasterMix (SMART-Seq v5 Ultra Low Input RNA Kit for Sequencing, Takara Bio). cDNA was amplified using 8 µl of the PCR MasterMix (SMART-Seq v5 Ultra Low Input RNA Kit for Sequencing ...
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bioRxiv - Immunology 2021Quote: ... Full length cDNA were generated from 4 ng of total RNA using Clontech SMART-Seq v4 Ultra Low Input RNA kit for Sequencing (Takara Bio Europe ...
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bioRxiv - Microbiology 2021Quote: ... 5′-RACE cDNA was obtained from bulk-sorted splenic B cells of each mouse with the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (TaKaRa). The IgG PCRs were set up with Platinum Taq High-Fidelity DNA Polymerase (Life Technologies ...
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bioRxiv - Evolutionary Biology 2021Quote: ... then removed from the pGEM shuttle plasmid using EcoRI and NotI enzymes and annealed into the DFRS empty plasmid pre-amplified (DFRS empty plasmid primers) and digested by the same enzymes (In-Fusion Kit; Clontech). The control cassette was thus placed on the 3’UTR of the RFP gene.
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bioRxiv - Neuroscience 2020Quote: ... The RNA was then converted into cDNA and prepared into RNA-seq libraries using the SmarterStranded kit (Takara; Cat#634843). The libraries were size selected for an average fragment size of 300 bp using SPRI beads (Beckman Coulter Life Sciences ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA was synthesized and amplified from 100 pg–300 pg of input RNA using SMART-seqTMv4 Ultra Low input RNA kit (Takara Clontech). Sequencing libraries were prepared using the Nextera XT DNA library preparation kit (Illumina) ...
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bioRxiv - Developmental Biology 2021Quote: 1000 FACS-purified cells from TgBAC(tcf21:H2B-Dendra2)ox182 larvae were processed using the SMART-SeqTm v4 UltraTm Low Input RNA Kit for Sequencing (Takara Clontech). Samples were lysed and poly-adenylated RNA reverse transcribed via SMARTScribe Reverse Transcriptase ...
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bioRxiv - Neuroscience 2021Quote: ... were reverse transcribed to complementary DNA (cDNA) using both oligo dT and random primers with PrimeScript RT Reagent Kit (Takara) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... 500 ng of total RNA was reverse transcribed (complementary DNA (cDNA) was synthesized using PrimeScript™ RT-PCR Kit (Takara) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: The target fragments are amplified by PCR using primers (Sangon Biotech, Shanghai, China) and PCR amplification kit (Takara Bio, Japan). The PCR amplifies the DNA fragments including the 1900 bp target ...
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bioRxiv - Neuroscience 2021Quote: ... Lentivirus was concentrated from supernatant using ultracentrifugation and the genome copy number was calculated using the Clontech Lenti-X qRT-PCR Titration kit (Takara).
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bioRxiv - Neuroscience 2021Quote: ... Reverse transcription and cDNA pre-amplification were performed using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech/Takara). cDNA was harvested and quantified with the Bioanalyzer DNA High-Sensitivity kit (Agilent Technologies) ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were FACS-sorted on a Becton Dickinson FACS Aria cell sorter gating for DAPI−/DRAQ5+/GFP+ cells directly collected in 100 μl of RA1 lysis buffer with 2 μl tris(2-carboxyethyl)phosphine (TCEP) from NucleoSpin RNA XS kit (Clontech).
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bioRxiv - Immunology 2021Quote: ... 5’ s-RACE cDNA was obtained from bulk-sorted B cells of each mouse with the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (TaKaRa). The IgG PCRs were set up with Platinum Taq High-Fidelity DNA Polymerase (Life Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... Total RNA (5ng) was then reverse transcribed using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech), which generates high-quality cDNA from ultra-low amounts of total RNA ...
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bioRxiv - Cancer Biology 2021Quote: ... including a puromycin resistance cassette and a tet-responsive element for expression of shRNAs was established according to a publicly available protocol (46) using In-Fusion HD Cloning Kit (Clontech) (Supplementary Table 3) ...
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bioRxiv - Cancer Biology 2020Quote: ... Total RNAs of 0.5 to 1 μg were used as templates for the reverse transcription using PrimeScriptTM RT Reagent Kit with gDNA Eraser (Takara). Quantitative PCR (qPCR ...
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PPARγ is a tumor suppressor in basal bladder tumors offering new potential therapeutic opportunitiesbioRxiv - Cancer Biology 2019Quote: ... The GAL4 DNA-binding domain cloning vector pM and the activation-domain cloning vector pVP16 are part of the Mammalian Matchmaker Two-Hybrid Assay kit (BD Biosciences Clontech). The construct pM-MED1 (510-787 ...
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bioRxiv - Cell Biology 2020Quote: ... The plasmid pJYB240 was generated from pJYB212 by exchanging the mEos2 open reading frame by a PCR-amplified mTurquoise2 DNA (Goedhart et al. 2012) using the InFusion kit (Clontech). The parBF-mTurquoise2 gene from pJYB240 was introduced into pJYB243 ...
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bioRxiv - Plant Biology 2021Quote: ... and were used for cDNA synthesis and library preparation using SMARTer universal low input RNA kit (Clontech, Mountain View, CA). Library fragment size distribution was checked on the Agilent 2100 Tapestation System with the High Sensitivity D1000 Kit (Agilent Technologies) ...
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bioRxiv - Biochemistry 2021Quote: ... DNA was gel purified and inserted into pCDNA 3.1 vectors (CMV promoter) by making use of In-Fusion HD Cloning Kits (Takara Bio) as described in previous section ...
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bioRxiv - Biochemistry 2021Quote: ... DNA was gel purified and inserted into pCDNA 3.1 vectors (CMV promoter) by making use of In-Fusion HD Cloning Kits (Takara Bio). EcoR1 and HindIII digested vector was incubated with overlapping PCR fragments (of various different recombinant DNAs ...
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bioRxiv - Cell Biology 2021Quote: Cell lysis was assessed by measuring Lactate Dehydrogenase (LDH) activity in cell supernatants using the LDH cytotoxicity detection kit (TaKaRa). To obtain the positive control ...
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bioRxiv - Plant Biology 2020Quote: ... and reverse-transcribed with T7-oligo(dT)24 as a primer using the PrimeScript II 1st strand cDNA Synthesis kit (TaKaRa). Then the poly(A ...
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bioRxiv - Plant Biology 2020Quote: ... mRNA and miRNA from three independent biological samples were reverse transcribed into cDNA using the PrimeScript II 1st Strand cDNA Synthesis Kit (TaKaRa) and the miRcute Plus miRNA First-Stand cDNA Synthesis Kit (Tiangen) ...
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bioRxiv - Microbiology 2020Quote: ... The viral load was measured by RT-qPCR using One-Step SYBR® Primescript™ RT-PCR kit II (Takara). CT values of serum samples were used to calculate serum viral titre according to regression equation built by RNA extracted from 10 µL of 102-106 pfu/mL of ZIKV (PRVABC59 or MP1751) ...
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bioRxiv - Plant Biology 2020Quote: For β-galactosidase assay constructs were processed according to the instructions of the β-galactosidase assay kit provided by Clontech. For each construct ...
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bioRxiv - Microbiology 2020Quote: ... Double stranded cDNA was prepared from 1 μg of each mRNA sample using the PrimeScript Double Strand cDNA Synthesis Kit (Takara) and a cDNA library was established with Ligation Sequencing Kit (SQK-LSK109 ...
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bioRxiv - Microbiology 2020Quote: ... Japan) and then mRNA was purified from total RNA by Oligotex −dT30 Super mRNA Purification Kit (Takara Bio, Kusatsu, Japan). Double stranded cDNA was prepared from 1 μg of each mRNA sample using the PrimeScript Double Strand cDNA Synthesis Kit (Takara ...
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bioRxiv - Neuroscience 2020Quote: ... We constructed the T2A-QF2-9xQUAS-GCaMP6f-3XP3-dsRed donor plasmid (Fig. 2a) using the InFusion HD Kit (Clontech, 638910). To preserve the orco coding sequence ...
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bioRxiv - Microbiology 2021Quote: Viral RNA was extracted from 200 μL of pseudovirus using the MiniBEST Viral RNA/DNA Extraction Kit Ver.5.0 (TaKaRa, Japan), and the extracted mRNA was used as a template for reverse transcription using M-MLV reverse transcriptase (Promega ...