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Citations for Takara Bio :
301 - 350 of 846 citations for SARS CoV 2 Spike N Terminal Domain NTD Sheep Fc Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: Total RNA was extracted from segments of the terminal ileum and colon and purified using TRIzol reagent (Takara Bio, Shiga, Japan). RNA was reverse transcribed into cDNA with PrimeScriptTM RT Master Mix (Takara Bio ...
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bioRxiv - Plant Biology 2021Quote: ... Products were ligated to pCAMBIA1300 vector backbone containing the 35S promoter and a C-terminal YFP using the In-Fusion cloning system (Takara Bio).
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bioRxiv - Immunology 2021Quote: ... An N-terminal-AcGFP-fused CDC42 expression vector was constructed by incorporating PCR-amplified CDC42 cDNA into the pAcGFP1-C1 Vector (Takara Bio). Each CDC42 variant was generated by PCR-based mutagenesis using KOD plus (Toyobo) ...
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bioRxiv - Cell Biology 2022Quote: ... and the PCR product was subcloned into the pcDNA3.1 (+) C-terminal PA vector (digested with KpnI/XhoI) using an In-Fusion HD Cloning Kit (#Z9648N; TaKaRa, Kusatsu, Japan), resulting in pcDNA3.1(+ ...
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bioRxiv - Molecular Biology 2020Quote: ... JEG3 cells (human; sex: female, placenta epithelial), HEK293 derivative Lenti-X™ 293T cells (human; sex: female, kidney epithelial) obtained from Takara (cat. 632180), Huh-7.5 heptoma cells (human ...
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bioRxiv - Genomics 2021Quote: Hap1 cells were cultured in Iscove’s Modified Dulbecco’s Medium (IMDM) supplemented with 10% FCS (Clontech), 1% Penicillin/Streptomycin (Invitrogen ...
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bioRxiv - Developmental Biology 2024Quote: ... Each sample was directly lysed in 4 μL of lysis buffer (including 0.2 μL of 1:1000 diluted external RNA controls consortium (ERCC) spike-in) and immediately reverse-transcribed using the PrimeScriptTM II Reverse Transcriptase (Takara, Cat# 2690A), and the cDNA library was constructed as the published Smart-seq2 method ...
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bioRxiv - Cancer Biology 2021Quote: ... and the oligonucleotides were inserted into C terminal of ARL4C-EGFP or ARL4C-FLAG-HA using In-Fusion HD Cloning Kit (Takara Bio Inc.).
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bioRxiv - Biophysics 2019Quote: ... D40A mutation and deletion of C-terminal 10 amino acids were introduced into pET15-sfGFP-minD by using the PrimeSTAR Max mutagenesis protocol (TaKaRa, Shiga, Japan). Similarly ...
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bioRxiv - Biochemistry 2019Quote: ... and a H2A histone lacking the C-terminal residues 110-130 were cloned using In-Fusion® HD cloning strategy (Takara Bio).
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bioRxiv - Microbiology 2019Quote: ... The NusA tag of GfsB was cleaved with a HRV 3C protease at 4°C (Takara Bio) and removed by Ni-agarose ...
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bioRxiv - Genetics 2020Quote: ... in frame with Myc and Flag tag by In-Fusion HD EcoDry Cloning Plus System (Takara Bio). In order to insert a poly histidine tag for protein expression and purification ...
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bioRxiv - Immunology 2023Quote: ... Random primer (Hexadeoxyribonucleotide mixture, pd(N)6) (3801) was purchased from Takara Bio ...
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bioRxiv - Molecular Biology 2019Quote: HEK293 cells were transfected with p122RhoGAP/DLC-1 siRNA (sense, 5′-GAAACGCCUUAAGACACUATT-3′; antisense, 5′-UAGUGUCUUAAGGC GUUUCTT-3′; TaKaRa Biotechnology Co., Ltd., Kyoto, Japan), IQGAP1 siRNA (s16837 ...
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bioRxiv - Neuroscience 2023Quote: ... 1.25 x105 cells/cm2 were immediately added to each well with HEK293 maintenance media modified with 10% tetracycline-free FBS (Takara Bio, Cat. No. 631367). After 24 h ...
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bioRxiv - Microbiology 2021Quote: ... SARS-CoV2 Mpro and C300S Mpro were purified first by affinity chromatography using TALON™ cobalt-based affinity Resin (Takara Bio). The His6-tag was cleaved off by PreScission protease and the resulting authentic 306 amino acid Mpro (see Figure S1A in supplemental material ...
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bioRxiv - Developmental Biology 2019Quote: ... The CDS of the EGFP and mCherry fluorescent tags were obtained by PCR from the pEGFP-C1 (Clontech) and pRSET-B-mCherry (Invitrogen ...
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bioRxiv - Plant Biology 2022Quote: ... Purification of His-tagged proteins was carried out using His-tag affinity resin (His-resin) (Clontech, CA, USA).
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bioRxiv - Microbiology 2022Quote: ... CydDC was purified via His6-tag affinity chromatography using a Talon (Co-IMAC) affinity matrix (Takara Bio Inc.). For column conditioning and washing step I (10 column volumes [CV] ...
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bioRxiv - Plant Biology 2019Quote: ... and 0.6 μl of 100 μM random primer (N)6 (TaKaRa, Kusatsu, Japan), with RNase-free water added up to 20 μl ...
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bioRxiv - Cell Biology 2022Quote: ... FcγRIIA cDNA was cloned into a pEGFP-N vector (Takara Bio USA, CA). FTractin cDNA was fused with Halo tag in pTriEx-4 vector (Millipore Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... The PCR fragments of spike protein DNA were cloned into linearized pMRNAXP vector using In-Fusion® HD Cloning Kit (Clontech® Laboratories, Inc.). The cloning mixtures were transformed to One Shot™ TOP10 Chemically Competent E ...
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bioRxiv - Synthetic Biology 2023Quote: ... 2 µL of 2 mM dNTPs (Takara #4025), and 0.6 µL of 100% DMSO (NEB #12611P) ...
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bioRxiv - Synthetic Biology 2023Quote: ... 2 µL of 2 mM dNTPs (Takara #4025), and 0.6 µL of 100% DMSO (NEB #12611P) ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 (TaKaRa) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 (TaKaRa) and purified using ProbeQuant G-50 Micro Columns (GE Healthcare ...
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bioRxiv - Biophysics 2021Quote: ... Constructs containing both His and Strep tags were purified using gravity flow columns containing His60 Ni-NTA resin (Clontech) followed by Streptactin affinity chromatography (IBA Lifesciences ...
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bioRxiv - Developmental Biology 2020Quote: ... and Zfp131 cDNAs were fused to 1x hemagglutinin (HA) tag and cloned into p2lox plasmid using In-fusion (Clontech) cloning ...
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bioRxiv - Biochemistry 2022Quote: ... was added at the N-termini of EGFP and mCherry (Clontech, Mountain View, CA). For intramolecular FRET experiments ...
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bioRxiv - Molecular Biology 2022Quote: ... Pyridylamination of N-glycans was performed using a pyridylamination manual kit (Takara Bio Inc.) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: Syt1-Myc-tagged lentiviral constructs were generated using the pCMV-Myc-N vector (Clontech) containing full-length rat Syt1 and a preprotachykinin signal sequence cloned upstream of the Myc tag as described in ref.17 The F349A mutation was introduced using site directed mutagenesis (QuickChange ...
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bioRxiv - Cell Biology 2021Quote: ... cells were stably transfected with a plasmid encoding human full-length keratin 8 with an EYFP tag at its carboxyterminus (Windoffer et al., 2004; recloned into pEYFP-N1 (Clontech) with BamHI and EcoRI) ...
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bioRxiv - Microbiology 2019Quote: ... mIRE1-wt was PCR-amplified (without a myc tag) from pcDNA3-mIRE1-3xmyc (Stahl et al, 2013) and subcloned in pMSCVhyg (Clontech) using BglII and HpaI sites ...
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bioRxiv - Microbiology 2020Quote: ... Total viral DNA was extracted using Qiagen viral DNA extraction kit (QIAamp DNA Mini Kit, Hilden, Germany) and DNA polymerase Tag (Takara) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Plasmid pTAP-AFAP was constructed by cloning the gene fragment encoding AFAP-streptavidin-binding peptide-3xFLAG tag fusion protein (AFAP-SBP-3xFLAG, shorted as AFAP-SF) into mammalian expression vector pIRESpuro3 (Takara). SBP is a peptide with the length of 38 amino acids (Wilson et al. ...
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bioRxiv - Molecular Biology 2019Quote: ... The cell debris was removed upon centrifugation and the proteins were purified from the supernatant by His-tag affinity chromatography using Ni-NTA agarose beads (Clontech). The bound proteins were washed with lysis buffer containing 10 mM imidazole and then eluted with lysis buffer containing 250 mM imidazole ...
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bioRxiv - Biochemistry 2019Quote: ... The cell debris was removed upon centrifugation in a SS34 rotor at 4 °C (27,000 × g for 15 min) and the proteins were purified from the supernatant by His-tag affinity chromatography using Ni-NTA agarose beads (Clontech). The bound proteins were washed with lysis buffer containing 25 mM imidazole and then eluted with lysis buffer containing 500 mM imidazole ...
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bioRxiv - Genomics 2019Quote: ... we prepared whole genome sequencing libraries using 1 ng input from healthy volunteer samples using ThruPLEX Tag-seq (Takara Bio). We performed sequencing on HiSeq 4000 (Illumina ...
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bioRxiv - Neuroscience 2021Quote: ... AP-MDGA1 and AP-MDGA2 were generated by replacing the V5 tag of the V5-MDGA1 and V5-MDGA2 constructs respectively by the 14 amino acids AP tag (5’GGCCTGAACGAtATCTTCGAGGCCCAG AAGATCGAGTGGCACGAG3’) using the HD-In-Fusion kit (Takara). The linker 5’GGAGGATCAGGAGGATCA3’ was added after the AP tag ...
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bioRxiv - Biophysics 2020Quote: pDNA_HaloTag/SNAP-tag/GFP vectors encoding each target protein (see Note 1),which were inserted by In-Fusion HD Cloning Kit (Clontech) or Seamless Ligation Cloning Extract (SLiCE ...
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bioRxiv - Developmental Biology 2020Quote: ... This allowed the HA tags to be replaced with GFP (amplified with primers including the AatII sites from pEGFP-N1 (Clontech). Forward primer ...
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bioRxiv - Microbiology 2020Quote: ... and tandem repeat Sgo_R3-4 (aa 621– 789) were cloned downstream of a hexahistidine tag and 3C protease specific linker by the In-fusion method (Clontech; primer sequences listed in Supplementary Table 1) ...
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bioRxiv - Cancer Biology 2021Quote: ... and HRas was purified via its 6His affinity tag using immobilized metal affinity chromatography (TALON® Metal affinity resin (Takara)) followed by elution using an imidazole step gradient ...
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bioRxiv - Biochemistry 2023Quote: ... Tm1 FL for motility assays was cloned with a HisSUMO-SNAP-tag between SacI and HindIII sites of the pET11 vector by InFusion cloning (Takara). The SNAP-Tm1 1-335 truncation was made by site- directed mutagenesis of the SNAP-Tm1 FL plasmid using primers that inserted a stop codon after aa 335 as described above.
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bioRxiv - Microbiology 2023Quote: ... and cloning it into the EcoRI and EcoRV sites of pcDNA-EGFP-P2A in frame with the tag sequence by using an In-Fusion® HD Cloning Kit according to the manufacturer’s instructions (Takara). pcDNA-hAPOBEC1 was constructed by cloning the XhoI-HindIII fragments of pUC57-APOBEC1 ...
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bioRxiv - Biochemistry 2023Quote: Khc FL for in vitro studies was cloned with a HisSUMO-SNAP-3C-tag into MCS1 between BamHI and HindIII sites of the pFastBacDual vector by InFusion cloning (Takara). The Khc mutant constructs used for in vitro studies were generated by site-directed mutagenesis of the Khc FL plasmid using primers that either inserted a stop codon after aa 910 (Khc910 ...
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bioRxiv - Developmental Biology 2023Quote: ... G9 deletion mutants were generated by PCR amplification or overlap extension PCR and inserted in frame with the MYC tag into the pCS2-MT-NLS expressing vector using the In Fusion Protocol (ST0345, Takara). All constructs were confirmed by Sanger sequencing.
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Molecular basis of proteolytic cleavage regulation by the extracellular matrix receptor dystroglycanbioRxiv - Biochemistry 2024Quote: ... A Flag tag x3 was inserted between amino acids 489 and 490 and a HA tag was inserted between amino acids 724 and 725 using In-Fusion Cloning Mix (Takara).
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bioRxiv - Cancer Biology 2021Quote: ... 2 kit (Takara) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 (Takara, #6045) according to manufacturer’s protocols ...