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Citations for Takara Bio :
301 - 350 of 1618 citations for Mouse IgG2a Isotype Control Antibody HOPC 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: Stable 3T3 cells (ATCC) overexpressing zDHHC3 or green fluorescent protein (GFP) as a control were generated using the pLVX lentiviral system (Clontech). Cells were labeled by stable isotope labeling with amino acids in cell culture (SILAC ...
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bioRxiv - Molecular Biology 2019Quote: ... Fusion PCR was carried out to create an eYFP marker under the control of the 3xP3 promoter with an SV40 terminator from plasmids eYFP-mem (Clontech), pBac[3×P3-dsRed]CP-Gal4-GFY-SV40 (Lynd and Lycett 2012 ...
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bioRxiv - Cell Biology 2021Quote: ... Lentiviral vectors expressing KDELR2 or a control empty vector were titered using the Lenti-X p24 rapid titer kit (Takara). 8 × 105 SH-SY5Y in 2 mL growth media were plated in a 6-well plate ...
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bioRxiv - Plant Biology 2021Quote: ... medium and 10-fold dilutions of these cultures were dropped on SD control (−2) and selective medium additionally lacking His (−3) (Clontech). Empty vectors were used as negative controls ...
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bioRxiv - Neuroscience 2022Quote: Total RNA was prepared from cortical samples from control and cKO animals (3 animals/group) by using RNAiso-plus kit (Takara) and according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... TetON-IRE1-GFP MEFs were obtained by reconstituting Ire1-/- MEFs with GFP-tagged murine IRE1 under control of a ‘tight’ Tet-responsive element (Clontech) essentially as described (Bakunts et al ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Forward (control) and reverse (target) RNA probes were synthesized from plasmids amplified via PCR (Advantage HD Polymerase Kit, Takara Bio) using T7 or SP6 RNA Polymerases (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... 50 μg of DNA (either Asterix-TEV-Strep in pAWG or pAGW [transfection control]) was premixed with 15 μL of Xfect Polymer transfection reagent (Takara) in 500 μL Xfect buffer ...
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bioRxiv - Cell Biology 2022Quote: ... All above sequences along with MBNL1 and MBNL2 isoforms imaged in primary neurons were cloned downstream of GFP in a pEGFP-C1 plasmid backbone under control of a CMV promoter (Takara). Full-length kinesin sequences were amplified from mouse cDNA and inserted upstream of an mScarlet-containing plasmid in a pcDNA3.1 backbone under the control of CMV promoter.
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bioRxiv - Biochemistry 2022Quote: The Tet-On inducible expression construct pTRE3G-BI/GPR83-EGFP or pTRE3G-BI/GPR83-IRES-EGFP was transiently transfected into HEK293T cells together with the expression control vector pCMV-TRE3G (Clontech) using the transfection reagent Lipo8000 (Beyotime Technology) ...
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bioRxiv - Molecular Biology 2022Quote: ... Mammalian expression vector for Flag-EBNA1 contained B95-8 EBNA1 lacking the GA repeats (aa 101-324) under the control of CMV-3XFLAG promoter in a plasmid derived from pREP10 (Clontech) containing ...
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Ferroportin Depletes Iron Needed for Cell Cycle Progression in Head and Neck Squamous Cell CarcinomabioRxiv - Cancer Biology 2022Quote: The tet-inducible lentivirus vectors pLVX-TetOne-puro vector and pLVX-Tetone-puro-Lucif control vector were purchased from Takara Bio ...
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bioRxiv - Cancer Biology 2023Quote: ... LNCaP KO and LNCaP NT control cells were transiently transfected with 150ng of NF-kB luciferase reporter gene (NF-kB-luc, Clontech) and 3.5ng of Renilla using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... We previously described the generation of psiRNA-U6GFP::Zeo and the nonsense negative control psiRNA-U6GFP::Zeo-shNS from the U6 RNA Polymerase III promoter in pSIREN-shuttle (Clontech) and psiRNA- 7SKGFP::Zeo (InvivoGen ...
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bioRxiv - Microbiology 2022Quote: ... monoclonal antibody (Clontech) and peroxidase-labeled anti-mouse IgG (H + L ...
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bioRxiv - Microbiology 2021Quote: ... mouse anti GFP (#632381, Takara Bio Clontech), mouse anti Vinculin (#V9131-100UL ...
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bioRxiv - Microbiology 2021Quote: ... mouse anti GFP (#632381, Takara Bio Clontech), mouse anti Vinculin (#V9131-100UL ...
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bioRxiv - Cell Biology 2022Quote: ... mouse anti-GFP (Clone JL-8; Clontech) at 1:1,000 ...
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bioRxiv - Neuroscience 2021Quote: ... followed by an incubation overnight at 4°C with antiDsRed rabbit polyclonal primary antibody (1:1000, 632496 Takara Bio) diluted in a triton buffer (0.3% Triton X-100 diluted in PBS-DEPC) ...
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bioRxiv - Neuroscience 2021Quote: ... followed by an incubation overnight at 4°C with antiDsRed rabbit polyclonal primary antibody (1:1000, 632496 Takara Bio) diluted in a triton buffer (0.3% Triton X-100 diluted in PBS-DEPC) ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were analyzed on bleach agarose gels (0.06% bleach, 1% (w/v) agarose) for visualization of the RNA and on WB (anti-His antibody, Clontech) for visualization of His6-tagged Nsp1.
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bioRxiv - Developmental Biology 2020Quote: ... Primary antibodies for embryo staining were used at the following final dilutions: rabbit anti-DsRed (1:400, Clontech 632496), rat anti-tropomyosin (1:200 ...
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bioRxiv - Developmental Biology 2023Quote: ... They were then incubated O/N at 4°C with the corresponding primary antibody: anti-DsRed (1:500; Clontech), mouse anti-GFP ([1:100] ...
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bioRxiv - Molecular Biology 2023Quote: ... Equal loading of the GFP tagged E2F1 proteins was determined by the GFP antibody (Clontech, lot. 1404005; 1:2000) the secondary antibody anti-rabbit HRP (Na934v ...
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bioRxiv - Molecular Biology 2023Quote: ... and 5 μg of total RNAs were reverse-transcribed into cDNA at 42°C for 1 h in 20 μl reaction mixture containing mouse Moloney leukemia virus reverse transcriptase (PrimeScript, TAKARA BIO, Shiga, Japan) with random 6 primers (TAKARA BIO) ...
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bioRxiv - Microbiology 2021Quote: ... pBICP35-CE1–CE8 transient expression vectors under the control of the 35S promoter were constructed using an In-Fusion system (Clontech, TaKaRa). The fragment containing the cDNA of CE1 was amplified with the primers 35S_CE1_Fw and 35S_CE1_Rv ...
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bioRxiv - Immunology 2022Quote: ... for continuous fluorescence detection in a total volume of 10 μL of cDNA/control and gene-specific primers using SYBR Premix Ex Taq (TaKaRa Bio). The mRNA levels of the target genes were normalized relative to those of Gapdh using the following formula ...
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bioRxiv - Genetics 2021Quote: Isolated RNA from OSF and control tissues was immediately subjected to realtime RT PCR (using Takara PrimescriptTM one step realtime RT PCR kit) (Takara, Japan) analysis for detection of changes in expression of NQO1 mRNA according to the manufacturers protocol ...
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bioRxiv - Genetics 2020Quote: The total RNA of the two groups (control (LV) and albino mutant (HUA)) was extracted using a Plant RNA Kit (TaKaRa, Japan) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... into which all possible combinations of 36 assays and 144 samples (127 woodchip samples, 16 standards, and one no-template control) were loaded by the SmartChip MultiSample NanoDispenser (Takara Bio). In brief ...
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bioRxiv - Plant Biology 2022Quote: ... in pGADT7 were combined with each other and with empty control plasmids using the Matchmaker™ Gold Yeast Two-Hybrid System (Clontech). The vectors were co-transformed into the Y2Hgold MATa Yeast strain ...
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bioRxiv - Microbiology 2023Quote: 0.35×105 293TT cells per plate were plated in 24-well plates 16-20 h prior to transfection of plasmids encoding HA-tagged CA Rab9a or the empty vector as control (Takara, 632105). 48 h after transfection ...
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bioRxiv - Genomics 2023Quote: ... of the resulting ligations as well as the control were transformed into 10 ul of Stellar chemically competent cells (Takara #636763) using manufacturer guidelines ...
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bioRxiv - Cancer Biology 2023Quote: ... pBH001 is comprised of a tetracycline-regulated bidirectional promoter for inducible expression of both firefly luciferase (a fragment cloned from the pTRE3G-BI-Luc control plasmid (Takara Bio)) and a recoded human L1 sequence (encoding both ORF1 and ORF2 ...
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bioRxiv - Neuroscience 2023Quote: ... we first produced a TRE3G-FlpO AAV carrying the optimised FlpO recombinase under the control of the third generation of tetracycline responsive element containing promoter (TRE3G, Clontech Laboratories). For this ...
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bioRxiv - Immunology 2023Quote: ... The damaged lung areas of the infected mice and the corresponding lung areas of the uninfected control mice were excised to isolate total RNAs using TaKaRa MiniBEST Universal RNA Extraction Kit (TaKaRa, 9767), which were reverse transcribed into cDNAs using the RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Following bulk BCR and TCR are prepared using SMARTer Mouse BCR IgG H/K/L Profiling Kit and SMARTer Mouse TCR a/b profiling kit separately (Takara). Based on the extracted mRNA amount of each sample ...
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bioRxiv - Molecular Biology 2020Quote: AQ-seq (bias-minimized sRNA-seq) libraries were constructed using total RNAs from fifteen mouse tissues (Mouse Total RNA Master Panel; Takara) as described in our previous study (Kim et al. ...
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bioRxiv - Microbiology 2021Quote: ... mouse anti-GFP (JL-8 clone, Takara Bio) or mouse anti-alpha-tubulin (Sigma) ...
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bioRxiv - Molecular Biology 2020Quote: ... RNAs from Mouse Total RNA Master Panel (Takara) were reverse-transcribed using the RevertAid First Strand cDNA Synthesis Kit (Thermo Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... we used the normalized mouse brain library (Clontech Takara Bio ...
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bioRxiv - Neuroscience 2021Quote: ... 1% normal donkey serum) for 1h at room temperature followed by incubation in primary antibodies (rabbit anti Ds-red, Takara, cat# 632496 ...
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bioRxiv - Neuroscience 2019Quote: ... the slices were incubated overnight at 4°C with a rabbit polyclonal antibody against DsRed (1:1000, Takara Bio USA) diluted in a blocking solution of 0.3% Triton X-100 in PBS-DEPC ...
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bioRxiv - Neuroscience 2022Quote: ... Incubation with primary antibodies was performed in blocking buffer containing rabbit anti-DsRed (1:1000, 632496; Clonetech-Takara Bio, Japan) antibody at 4°C for 1–2 days ...
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bioRxiv - Neuroscience 2020Quote: Primary antibodies used in this study include rabbit anti-DsRed (1:2000; #632496; Takara Bio USA, Inc., Mountain View, CA), rabbit anti-mCherry (1:500 ...
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bioRxiv - Cancer Biology 2023Quote: ... slides were incubated overnight at 4°C with primary antibody diluted in blocking buffer (these were: 1:200 Living Colors Ab, #632380, Takara; 1:100 L-plastin ...
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bioRxiv - Microbiology 2023Quote: ... anti-GAPDH antibody (Clontech) at a 1/2500 dilution ...
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bioRxiv - Genomics 2020Quote: ... total RNA of midgut samples in treatment groups and control groups were respectively extracted using AxyPrep™ Multisource Total RNA Miniprep Kit (TaKaRa, Japan); (2 ...
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bioRxiv - Genetics 2020Quote: ... and the reverse tetracycline transactivator (rtTA) and a neomycin resistance gene under the control of an EF1α promoter (ClonTech, Mountain View, CA), as previously described57 ...
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bioRxiv - Developmental Biology 2024Quote: ... Each sample was directly lysed in 4 μL of lysis buffer (including 0.2 μL of 1:1000 diluted external RNA controls consortium (ERCC) spike-in) and immediately reverse-transcribed using the PrimeScriptTM II Reverse Transcriptase (Takara, Cat# 2690A), and the cDNA library was constructed as the published Smart-seq2 method ...