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Citations for Takara Bio :
301 - 350 of 604 citations for Methylphosphonic Acid 13C 99%; Methyl D3 98% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... cell content was ejected onto 1.1 µl drop of lysis buffer (0.1% Triton X-100, 2 U/µl TaKaRa RNAse inhibitor ...
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bioRxiv - Cell Biology 2020Quote: ... 100 ng of total RNA was reverse-transcribed using the PrimeScript RT Reagent Kit (Takara Bio, Shiga, Japan) and a T100 thermal cycler (Bio-Rad Laboratories ...
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bioRxiv - Systems Biology 2022Quote: ... and deposited in 10 uL ice-cold lysis buffer containing 0.1% Triton-X 100 and RNase inhibitor (Takara) and stored at −80°C until further processing ...
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bioRxiv - Physiology 2021Quote: ... mixed with 5 μl lysis buffer (0.2% Triton X-100, with 2 U/μl recombinant RNase inhibitor, Clontech) at the end of the collection and were immediately snap-frozen on dry ice ...
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bioRxiv - Microbiology 2020Quote: ... The pSMART BAC-T7-scv2-replicon-Amp DNA was prepared using NucleoBond BAC 100 kit (Takara Cat# 740579). A fragment containing the RdRp D760N D761N mutation sequence was amplified by PCR using primers 5’-CGTAAACATTTCTCAATGATGATACTCTCTAACAATGCTGTTGTGTGTTTCAATAG-3’ and 5’-CAAAGAACACAAGCCCCAACAGCCTGTAAGACTGTATGCGGTGTGTACATAGC-3’ ...
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bioRxiv - Biophysics 2022Quote: ... 10 μL of the reaction was then transformed into 100 μL of StellarTM competent cells (Takara Bio USA) and plated on kanamycin plates ...
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bioRxiv - Neuroscience 2023Quote: ... the cell body was immediately sucked into the glass electrode with negative pressure and expelled onto a 1.1 µl drop of the ice-cold lysis buffer (0.1% Triton X-100, 2 U/µl TaKaRa RNAse inhibitor ...
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bioRxiv - Neuroscience 2023Quote: ... by dispensing individual cells in 5 nL drops directly into 3 µL ice-cold single cell lysis buffer (scLB, 0.134% Triton X-100 [Sigma], 0.5 U/µL recombinant RNase inhibitor [Takara, 2313B] ...
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bioRxiv - Genomics 2023Quote: ... 100 ng to 1 µg of total RNA was used for Hi-Mammalian whole transcriptome preparation (Takara Bio) and sequencing was performed on Nextseq2000 instrument with 1 x 72 bp single-end setup ...
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RNA-binding protein YBX1 promotes Type H vessels dependent bone formation in an m5C-dependent mannerbioRxiv - Molecular Biology 2023Quote: ... The paraffin sections were de-waxed and stained with primary antibody OCN (#M173, Takara Bio, Japan, 1:100), and counterstained with Harris Hematoxylin.
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bioRxiv - Cell Biology 2023Quote: ... media containing lentivirus was collected and concentrated 100-fold using the Lenti-X Concentrator (Takara Bio, Cat# 631231). Lentiviral titers were determined using the qPCR Lentivirus Titration Kit (abm ...
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bioRxiv - Microbiology 2023Quote: ... The PCR reaction mixture (100 nL) was prepared using SmartChip TB Green Gene Expression Master Mix (TaKaRa Bio), nuclease-free PCR-grade water ...
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bioRxiv - Cell Biology 2024Quote: ... Genomic DNA was purified from at least 100 million cells of each sample using NucleoSpin Blood XL (Takara) or QIAamp DNA Blood Midi Kit (QIAGEN) ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA was diluted to 100 ng/μl and was mixed with Green Premix Ex Taq II (Takara Bio, China) and amplifications were carried out following the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... 48hrs later the virus-containing supernatant from HEK-293T cells was concentrated 100-fold using Lenti-X concentrator (Takara). Titration was performed on HEK293T cells (ATCC ...
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bioRxiv - Neuroscience 2022Quote: ... coli cells induced with 100 mM IPTG and purified from bacterial extracts using TALON beads (Takara Bio, Catalog # 635502) according to the vendor’s instructions.
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bioRxiv - Microbiology 2020Quote: ... The supernatant was filtered through a 0.45μm PES filter and the viral particles concentrated 100 times using Lenti-X™ Concentrator (Takara) before storage at −80 ...
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bioRxiv - Molecular Biology 2022Quote: ... the cell pellets were resuspended in the prepared cell lysis buffer (0.1% Triton-X, 20 mM Tris, 100 mM NaCl) and ProteoGuard EDTA-Free protease inhibitor cocktail (Takara) for 30 minutes followed by sonication in ice cold water for 40 minutes (75 amplitude ...
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bioRxiv - Biochemistry 2021Quote: ... After incubation for 2 hours at 4°C the resin was washed extensively with buffer B and the flWT-Kv3.1 tetramer was eluted by addition of 100 µl of HRV 3C Protease at 1U/µl concentration from Takara Bio ...
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bioRxiv - Microbiology 2023Quote: ... and an aliquot of 100 ng was reverse transcribed with the PrimeScript RT Reagent Kit with gDNA Eraser (Takara) and the included primer mix ...
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bioRxiv - Developmental Biology 2022Quote: ... followed by the addition of 2.8 μL quenching buffer (0.7 μL of Triton X-100 [Nacalai Tesque], 0.7 μL of 2% bovine serum albumin [BSA] [Takara Bio]) and incubation at 70°C for 90 sec to quench the denaturing effect of SDc ...
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bioRxiv - Neuroscience 2023Quote: ... A total of 100 microglia from each region were collected in 5 μL single-cell lysis buffer (635013, Takara), and flash-frozen on dry ice.
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bioRxiv - Neuroscience 2024Quote: ... After the addition of 5 µl lysis buffer (0.2% Triton X-100, with 2 U/μl recombinant RNase inhibitor, Clontech) to the cap ...
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bioRxiv - Molecular Biology 2023Quote: ... 100 ng of each RNA sample was reverse-transcribed with PrimeScript RT Master Mix (Perfect Real Time) (Takara Bio). qPCR was performed using the QuantiTect SYBR Green PCR Kit (QIAGEN) ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was then pre-hybridized in 10 mL ExpressHyb (Clontech, NC9747391) at 44°C in a rotating oven for 1 hour ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 mM DTT and 400 units/ml of Recombinant RNase Inhibitor (TaKaRa)) with cOmplete (Roche ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 ng/ml of doxycycline (Takara Bio USA, Mountain View, CA, USA) were added to each cell lines and cultured for 48 h.
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bioRxiv - Biochemistry 2021Quote: ... The resin was then loaded on a reusable column (20 ml, Clontech) and washed with 20 ml of binding buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... NLRP3 expression was induced by adding 0.5 μg/mL doxycycline (Takara Bio) to the cell culture medium.
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bioRxiv - Biochemistry 2021Quote: ... clarified supernatants were purified using a 5 ml Cobalt affinity column (Takara). HCoV-OC43 S was purified using a StrepTrap HP column (GE healthcare) ...
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bioRxiv - Cell Biology 2021Quote: ... before loading into a TALON® 2 ml Gravity Column (Takara 635606). Columns were washed one additional time before elution in a single step (150 mM Imidazole ...
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bioRxiv - Neuroscience 2020Quote: ... and the supernatant was mixed with Ni-NTA resin (Clontech, 3 mL) and kept on a rocker at RT for 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... 2.5 mM dNTP and 2 U/mL of recombinant RNase inhibitor (Clontech) then spun down and frozen at −80°C ...
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bioRxiv - Biochemistry 2019Quote: ... the supernatant was rotated with 1.5 mL of TALON Cobalt resin (Clontech) for 30 min at 4°C in the presence of 5 mM imidazole ...
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bioRxiv - Biochemistry 2019Quote: ... The supernatant was rotated with 0.5 mL of TALON Cobalt resin (Clontech) for 30 min at 4°C in the presence of 5 mM imidazole ...
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bioRxiv - Biochemistry 2021Quote: ... Expression of CLPTM1L-mEGFP was induced by 1 µg/mL doxycycline (TaKaRa) and maintained in DMEM supplemented with Tet System Approved FBS (631107 ...
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bioRxiv - Immunology 2020Quote: ... pre-coated with retronectin (50 μg/mL, Takara Bio. Inc., Shiga, Japan). Activated T cells were spinoculated at 32 °C at 750 g for 60 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were then treated with 2 μg/mL puromycin (Clontech; 631306) under selection for at least 1 week ...
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bioRxiv - Molecular Biology 2023Quote: ... Cleared lysates were applied to 3 mL TALON Metal Affinity Resin (Takara) and incubated for 30 minutes at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... supplemented with 1 ml Lenti-X Concentrator (Takara Bio; Cat. No. 631231), mixed thoroughly ...
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bioRxiv - Developmental Biology 2023Quote: ... and TetON-regulated transgenes were activated by 2µg/ml doxycyclin (Clontech #63131l) medium supplementation at DIV4.
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was applied on a Talon affinity column (10 ml; Clontech) equilibrated in buffer A ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was incubated with 2 ml Ni-IDA resin (Takara Bio) for 2 hrs at 4°C ...
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bioRxiv - Synthetic Biology 2023Quote: ... polystyrene plates were coated overnight with 20 µg/mL retronectin (Takara Bio.) in PBS at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... supplemented with 6 ml Lenti-X Concentrator (Takara Bio; Cat. No. 631231), mixed thoroughly ...
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bioRxiv - Bioengineering 2023Quote: ... digested in 50 mg/mL Yatalase in PBS (Takara Bio, Cat# T017) for 40 minutes at RT and rinsed 3 times with PBS ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was then pre- hybridized in 10 mL ExpressHyb (Clontech, NC9747391) at 44°C in a rotating oven for 1 hour ...
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bioRxiv - Cancer Biology 2023Quote: ... then cells were selected with 2 μg/ml puromycin (631306; Takara Bio) or 500 μg/ml geneticin (10131027 ...
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bioRxiv - Plant Biology 2019Quote: ... The first-strand cDNA for qRT-PCR was synthesized from 100 ng total RNA using PrimeScript RT reagent Kit with gDNA Eraser (TaKaRa). For qRT-PCR ...
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bioRxiv - Genomics 2019Quote: ... reverse transcription was initiated from the bridge 3’ OH by adding 2 μL SMARTScribe Reverse Transcriptase (100 U/ μL, Clontech) and incubating for 1 h at 42 °C with shaking at 800 rpm ...