Labshake search
Citations for Takara Bio :
301 - 313 of 313 citations for D Fructose U 13C6 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... was amplified from Tak-1 genomic DNA by PCR using KOD plus with the primers in Table S1 and cloned into the unique NotI site of pENTR/D-TOPO-MpARK by In-Fusion system (Takara) to generate pENTR/D-TOPO-MpARKpro:MpARK ...
-
bioRxiv - Plant Biology 2023Quote: ... and was cloned into the AscI site of the pENTR/D-TOPO entry vector containing full length MpNEK1 CDS (Otani et al., 2018) by In-fusion system (Takara). The resulting vector pENTR/D-TOPO-MpNEK1-mCitrine was subjected to LR reaction to transfer MpNEK1-Citrine fusion into the Gateway binary vector pMpGWB144 (MpEF1α pro:XVE >>LexA operator:Gateway cassette).
-
bioRxiv - Plant Biology 2024Quote: The full-length FLP1 cDNA in pENTR/D-TOPO was cloned into pDEST_GBKT7 vector85 by Gateway reaction and transformed into Y2HGold yeast strain (Takara Bio). The resultant yeast was cultured in synthetic defined medium (–W) ...
-
bioRxiv - Genomics 2020Quote: ... RNase Inhibitor (0.4 U) and 1.92 μM of the 3’ oligo dT terminating primer: SMART-Seq® ICELL8® CDS (Takara Bio USA, CA, USA).
-
bioRxiv - Genomics 2020Quote: ... 5X Primer Mix (P5 and P7 primers) and Terra™ PCR Direct Polymerase Mix 0.05 U/ µl at reaction (Takara Bio USA, CA, USA) via a thermal protocol ...
-
bioRxiv - Genetics 2024Quote: ... Total RNA was extracted from the leaves using the Acid Phenol Guanidium Chloroform method (Chomczynski and Sacchi 2006) and was then treated with 10 U of DNase I (Takara Bio Inc., Kyoto, Japan). poly(A)+ RNA was separated and purified from total RNA using an oligo(dT ...
-
bioRxiv - Physiology 2024Quote: ... with 2 ul of RNAse inhibitor mix (containing 0.12 ul Triton X-100 10 %, 0.1 ul RNAse inhibitor (Takara (Cat. No. 2313B, 40 U/ul), 1.78 ul MilliQ water (RNAse-free)) ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... (300 ng DNA, 2.4 nM primers, 200 μM of each dNTP, 1x PCR buffer and 1.25 U Takara LA Taq polymerase in 25 μl reaction). Specificity of PCR products was confirmed on a 1% agarose gel and purified with AMPure PB beads.
-
bioRxiv - Neuroscience 2023Quote: ... 5 μL of treated RNA was reverse transcribed using oligo d(T) primers (PrimeScript RT Reagent Kit, Takara Bio, Kyoto Japan). Then ...
-
bioRxiv - Bioengineering 2020Quote: ... cDNA was synthesized using a mix of random hexamers – oligo d(T) primers and PrimerScript reverse transcriptase enzyme (Takara bio inc. Kit), again following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... The β-galactosidase activity was determined by ONPG (o-nitrophenyl β-D-galactopyranoside) liquid assay (quantitative) as mentioned elsewhere (Clontech Laboratories, 2008). β-galactosidase activity from each sample was calculated from at least three biological replicates and the concentration of cells (O.D.600 ...
-
bioRxiv - Molecular Biology 2021Quote: ... pDUP50M1 or pDUP51-ΔM and pDUP50-ΔM (a kind gift from D. L. Black, UCLA, USA) and pCMS-EGFP (Takara Bio USA, Inc) or pEGFP-IQGAP1 (Ren et al. ...
-
bioRxiv - Neuroscience 2023Quote: ... total RNA was isolated by standard procedure from 20-30 heads of homozygous Aprt5 flies and reverse transcribed using oligo d(T) primers (PrimeScript RT Reagent Kit, Takara Bio, Kyoto, Japan). At least 750 ng of the first strand cDNA was amplified by PCR in 20 μl of reaction mixture using PrimeStar Max DNA polymerase (Takara Bio ...