Labshake search
Citations for Takara Bio :
3401 - 3450 of 5458 citations for Rat Insulin Like Growth Factor Binding Protein 4 IGFBP4 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2024Quote: ... and cDNA was synthesized using PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) (Takara) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2024Quote: ... and cDNA was synthesized from 1 μg RNA using PrimeScriptTM RT Master Mix kit (Takara, Cat#RR036A) following the manufacturer’s protocols ...
-
bioRxiv - Cell Biology 2024Quote: ... the first strand cDNA was prepared using PrimeScript II 1st Strand cDNA Synthesis Kit (Takara, Beijing, China).
-
bioRxiv - Cell Biology 2024Quote: ... Quantitative real-time polymerase chain reaction (qPCR) was performed using TB Green Premix Ex Taq Kit (Takara) on QuantStudio 6 Flex Real-Time PCR System (Life Technologies) ...
-
bioRxiv - Bioengineering 2024Quote: ... We confirmed the cells were mycoplasma-negative using a Cycleave PCR Mycoplasma Detection Kit (Takara Bio, CY232).
-
bioRxiv - Microbiology 2024Quote: The processed DNA was ready for Illumina library preparation using a SMART ChIP-seq kit (Takara, #634865) following the manufacturer’s protocol (Fig ...
-
bioRxiv - Microbiology 2024Quote: ... 5′ RACE was carried out using SMARTer RACE 5′/3′ Kit (Takara Bio USA, Mountain View, CA) to identify the transcription start site (TSS ...
-
bioRxiv - Neuroscience 2024Quote: ... immediately followed by reverse-transcriptions and cDNA amplification with the smart-seq HT kit (Cat# 634437, Takara). The post-amplified cDNAs (1 ng per sample ...
-
bioRxiv - Molecular Biology 2024Quote: ... The digested backbone and the two amplicons were joined by In-Fusion® HD Cloning Kit (Takara). The SFFV U3 in the pAS.SFFV.d2GFP.PRE plasmid was then exchanged for other U3 sequences by digesting the plasmid with XcmI and AscI (NEB ...
-
bioRxiv - Developmental Biology 2024Quote: ... RNA sequencing libraries were prepared using SMARTer Stranded Total RNA-Seq Kit v3 (Takara Bio USA, Inc) following the recommended protocol with minor adjustments ...
-
bioRxiv - Cell Biology 2024Quote: ... The lentiviral titer was then determined using the Lenti-X p24 rapid titer kit (Takara Cat #632200). The Tau-RD-P301S-K18 FRET Biosensor CRL-3275 were exposed to active recombinant P301S-MAPT fibrils (ab246003 ...
-
bioRxiv - Synthetic Biology 2021Quote: ... DmrA and DmrC were amplified and then fused to each split-Cas9 (N_Cas9 and C_Cas9) using the In-Fusion HD Cloning Kit (Clontech). Links to sequences of all the generated plasmids are listed in Supplementary Table 3.
-
bioRxiv - Cancer Biology 2021Quote: Full-length cDNA was synthesized from total RNA (1 μg) by SMARTer®□ PCR cDNA Synthesis kit (Clontech) according to the manufacture’s instruction ...
-
bioRxiv - Cancer Biology 2021Quote: Illumina sequencing libraries of isolated RPFs was performed with the SMARTer smRNA-Seq Kit for Illumina (Takara Bio). Library preparation was performed according to the manufacturer’s protocol with the following parameters ...
-
bioRxiv - Cell Biology 2020Quote: ... and reverse transcribed into cDNA using reagents from a Prime Script II first strand cDNA synthesis kit (Takara). Real-time PCR was performed with the reverse transcription products ...
-
bioRxiv - Cell Biology 2020Quote: ... Libraries were prepared from 8mL of RNA using the SMARTer Stranded Total RNA-seq Pico kit v2 (Clontech), per manufacturer instructions ...
-
bioRxiv - Cell Biology 2020Quote: ... 500 ng total RNA was reverse-transcribed to cDNA (Prime-Script RT reagent kit, Takara Bio, Dalian, China). The parameters set for reverse transcription were as follows ...
-
bioRxiv - Genetics 2021Quote: ... Strand specific RNA-seq libraries were then constructed using the SMARTer Stranded RNA-Seq HT Kit (Clontech #634839), according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2021Quote: ... Viral RNA was amplified using PrimeScript II High Fidelity One Step RT-PCR Kit (Takara Bio, Shiga, Japan) under the following reaction conditions ...
-
bioRxiv - Microbiology 2020Quote: ... cDNA was generated following the SMARTer® 5’/3’ RACE kit protocol (Takara Bio, Kusatsu, Shiga Prefecture, Japan).
-
bioRxiv - Developmental Biology 2021Quote: ... cDNA synthesis was completed by SMART-Seq™ v4 Ultra™ Low Input RNA kit for sequencing (Clontech). Samples were sequenced on Illumina machines ...
-
bioRxiv - Developmental Biology 2020Quote: ... Total RNA was converted to cDNA using PrimeScript RT reagent Kit with gDNA Eraser (RR047A, Takara, Dalian, China). The mRNA expression levels of the Ccl9 ...
-
bioRxiv - Developmental Biology 2020Quote: Total RNA was extracted from cells using the NucleoSpin RNA II kit (Clontech Laboratories, Mountain View, CA, USA). The quality and quantity of total RNA were measured by Nanodrop 1000 (Thermo Fisher Scientific) ...
-
bioRxiv - Synthetic Biology 2022Quote: Genomic DNA was extracted from sorted T cells using the Macherey-Nagel NucleoSpin Tissue XS kit (Takara #740901.250). DNA encoding the CAR costimulatory domain was amplified from the extracted genomic DNA using the forward primer 5’-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGNNNNNACTGGTTATCACCCTTTA CTGC-3’ (Integrated DNA Technologies ...
-
bioRxiv - Plant Biology 2022Quote: Total RNAs were extracted from various tissues using the MiniBEST Plant RNA Extraction Kit (Takara Bio, Beijing, China). First-strand cDNAs were synthesized using TransScript One-Step gDNA Removal and cDNA Synthesis SuperMix (TransGen Biotech ...
-
bioRxiv - Molecular Biology 2022Quote: The RNA samples extracted from the fruit flesh were reverse transcribed using the PrimeScript RT-PCR Kit (TaKaRa) and the oligo(dt)20 primer ...
-
bioRxiv - Microbiology 2022Quote: ... The first-strand cDNA was synthesized using reverse transcriptase from the PrimeScript RT reagent kit (Takara Biotechnology, Japan) according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2022Quote: ... were purchased from Biomatik and cloned into BstBI-digested PBRM1-TetO-FUW using the ligation-free In-Fusion HD cloning kit (Takara). The construct was packaged into lentivirus using HEK293T cells and delivered into target cells together with pLenti CMV rtTA3 Hygro (w785-1 ...
-
bioRxiv - Neuroscience 2021Quote: RNA from FACS-sorted cells ranging from 3,000 – 10,000 were isolated with the NucleoSpin RNA XS kit (Clontech) with on column DNase digestion according to the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2021Quote: ... cells and medium were collected and AAV particles were purified by AAVpro Purification Kit (Takara Bio; Shiga, Japan) according to the manufacturer’s instructions.
-
bioRxiv - Molecular Biology 2020Quote: ... The cDNAs used for RT-PCR were synthesized using PrimeScript First-Strand cDNA Synthesis Kits (Takara, Osaka, Japan). RT-PCR cycling conditions were as follows ...
-
bioRxiv - Molecular Biology 2020Quote: ... and one μg RNA was converted to cDNA by using the PrimeScript™ RT-PCR Kit (Takara, Japan) following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: ... then into pShuttle-CMV with EcoRV using the In-Fusion HD Cloning Plus System kit (Takara Bio USA). These were linearized with Pme1 and cotransformed with pAdEasy-1 into BJ5183 cells ...
-
bioRxiv - Cell Biology 2022Quote: cDNA was generated from single cells in the 96-well plate using the SmartSeq v4 kits (Takara Bio) using 1/4th volume reactions dispensed using a Mantis dispenser (Formulatrix) ...
-
bioRxiv - Immunology 2021Quote: ... cDNA was generated from polyadenylated transcripts employing the SMART-Seq v4 ultra low input RNA kit (Takara Bio). RNA and cDNA quality was analysed on a 2100 Bioanalyser (Agilent) ...
-
bioRxiv - Biochemistry 2019Quote: ... Lentivirus was produced by transient transfection of HEK 293T cells using the CalPhos mammalian transfection kit (Clontech laboratories) to introduce packaging vectors (VSVg and GAG/POL/Δ8.2 ...
-
bioRxiv - Molecular Biology 2019Quote: ... the cDNA was synthesized by reverse transcription using PrimeScript RT reagent kits with gDNA Eraser (Takara, Dalian, China) according to the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2019Quote: ... we cloned Pikp-HMANK-KE (as above) into pGBKT7 using an In-Fusion cloning kit (Takara Bio USA), following the manufacture’s protocol ...
-
bioRxiv - Immunology 2019Quote: ... cDNA was generated from 1 μg of RNA using the PrimeScript RT reagent kit with gDNA Eraser (Takara). The mRNA quantifications of target genes were performed by real-time PCR using the SYBR GREEN MIX (Takara) ...
-
bioRxiv - Pathology 2020Quote: ... An RNA library was then constructed using the SMARTer Stranded Total RNA-Seq Kit v2 (TaKaRa, Dalian, China). Ribosomal RNA (rRNA ...
-
bioRxiv - Cell Biology 2019Quote: ... The amplified products were ligated using ClonTech In-Fusion HD Cloning kit (Takara Bio USA, Mountain View, CA) and the resultant pBS-HsMyo1e construct contained MYO1E CDS flanked by myo1+ 5’ and 3’ UTRs ...
-
bioRxiv - Plant Biology 2019Quote: ... Full-length cDNA of LcCHI2 sequence was obtained using a SMART™ RACE cDNA Amplification Kit (Clontech, USA) for 3’ RACE with the primers 5′-CCGACCAGTTCCAATGGGGCT-3′ and 5′-GGCCACGCGTCGACTAGTACTTTTTTTTTTTTTTTTT-3′.
-
bioRxiv - Pathology 2019Quote: ... and treated with DNase I following the manufacturer’s instructions (PrimeScript RT reagent Kit with gDNA Erase, Takara, Japan). cDNA synthesized from reverse transcription of RNA samples was used to determine the mRNA expression levels of target genes as well as for quantifying TYLCV accumulation levels at the date indicated ...
-
bioRxiv - Microbiology 2019Quote: Cloning via homologous recombination was carried out using the In-Fusion® HD cloning kit (Clontech Laboratories Inc). Primers used can be seen in Table 2.
-
bioRxiv - Microbiology 2020Quote: ... Quantitative real-time PCR analysis was performed using the SYBR Premix Ex TaqTM II kit (Takara, Dalian, China). HMGB1 primers were designed using Primier4.1 software and GADPH was used as a reference gene ...
-
bioRxiv - Microbiology 2019Quote: ... the total RNA (1 µg) was reverse transcribed by PrimeScript RT reagent Kit with gDNA Eraser (Takara, China). The IL-1β and components of RISC mRNA expression levels of the NA cells were quantified with the SYBR Green qPCR kit (Takara ...
-
bioRxiv - Neuroscience 2019Quote: ... Three ng of RNA was used to prepare cDNA using SMART-Seq v4 Ultra Low Input Kit (Takara). Following cDNA preparation ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... coli as a DNA template) with the Neo-selection gene by using In-Fusion HD Cloning kit (Takara), and integrated the resulting leuS-Neo DNA into genomes of the tavaborole-resistant cells by using the scarred method for insertion of a nonselectable DNA fragment recombineering protocol with Neo gene as a selectable marker49.
-
bioRxiv - Cell Biology 2019Quote: ... approximately 6 μg RNA was applied for 1st-strand cDNA synthesis using PrimeScriptTM RT Reagent Kit (TaKaRa, AK6003) with oligo (dT ...
-
bioRxiv - Genomics 2020Quote: ... cDNA was generated via PrimerScriptTM RT Reagent Kit with genomic DNA Eraser (Perfect Real Time) (TaKaRa, Beijing, China). Quantitative real-time PCR analysis (q-PCR ...