Labshake search
Citations for Takara Bio :
3201 - 3250 of 5251 citations for Rat Activity Dependent Neuroprotector Homeobox Protein ADNP ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2020Quote: ... and one μg RNA was converted to cDNA by using the PrimeScript™ RT-PCR Kit (Takara, Japan) following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: ... then into pShuttle-CMV with EcoRV using the In-Fusion HD Cloning Plus System kit (Takara Bio USA). These were linearized with Pme1 and cotransformed with pAdEasy-1 into BJ5183 cells ...
-
bioRxiv - Cell Biology 2022Quote: cDNA was generated from single cells in the 96-well plate using the SmartSeq v4 kits (Takara Bio) using 1/4th volume reactions dispensed using a Mantis dispenser (Formulatrix) ...
-
bioRxiv - Immunology 2021Quote: ... cDNA was generated from polyadenylated transcripts employing the SMART-Seq v4 ultra low input RNA kit (Takara Bio). RNA and cDNA quality was analysed on a 2100 Bioanalyser (Agilent) ...
-
bioRxiv - Biochemistry 2019Quote: ... Lentivirus was produced by transient transfection of HEK 293T cells using the CalPhos mammalian transfection kit (Clontech laboratories) to introduce packaging vectors (VSVg and GAG/POL/Δ8.2 ...
-
bioRxiv - Molecular Biology 2019Quote: ... the cDNA was synthesized by reverse transcription using PrimeScript RT reagent kits with gDNA Eraser (Takara, Dalian, China) according to the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2019Quote: ... we cloned Pikp-HMANK-KE (as above) into pGBKT7 using an In-Fusion cloning kit (Takara Bio USA), following the manufacture’s protocol ...
-
bioRxiv - Immunology 2019Quote: ... cDNA was generated from 1 μg of RNA using the PrimeScript RT reagent kit with gDNA Eraser (Takara). The mRNA quantifications of target genes were performed by real-time PCR using the SYBR GREEN MIX (Takara) ...
-
bioRxiv - Pathology 2020Quote: ... An RNA library was then constructed using the SMARTer Stranded Total RNA-Seq Kit v2 (TaKaRa, Dalian, China). Ribosomal RNA (rRNA ...
-
bioRxiv - Cell Biology 2019Quote: ... The amplified products were ligated using ClonTech In-Fusion HD Cloning kit (Takara Bio USA, Mountain View, CA) and the resultant pBS-HsMyo1e construct contained MYO1E CDS flanked by myo1+ 5’ and 3’ UTRs ...
-
bioRxiv - Plant Biology 2019Quote: ... Full-length cDNA of LcCHI2 sequence was obtained using a SMART™ RACE cDNA Amplification Kit (Clontech, USA) for 3’ RACE with the primers 5′-CCGACCAGTTCCAATGGGGCT-3′ and 5′-GGCCACGCGTCGACTAGTACTTTTTTTTTTTTTTTTT-3′.
-
bioRxiv - Pathology 2019Quote: ... and treated with DNase I following the manufacturer’s instructions (PrimeScript RT reagent Kit with gDNA Erase, Takara, Japan). cDNA synthesized from reverse transcription of RNA samples was used to determine the mRNA expression levels of target genes as well as for quantifying TYLCV accumulation levels at the date indicated ...
-
bioRxiv - Microbiology 2019Quote: Cloning via homologous recombination was carried out using the In-Fusion® HD cloning kit (Clontech Laboratories Inc). Primers used can be seen in Table 2.
-
bioRxiv - Microbiology 2020Quote: ... Quantitative real-time PCR analysis was performed using the SYBR Premix Ex TaqTM II kit (Takara, Dalian, China). HMGB1 primers were designed using Primier4.1 software and GADPH was used as a reference gene ...
-
bioRxiv - Microbiology 2019Quote: ... the total RNA (1 µg) was reverse transcribed by PrimeScript RT reagent Kit with gDNA Eraser (Takara, China). The IL-1β and components of RISC mRNA expression levels of the NA cells were quantified with the SYBR Green qPCR kit (Takara ...
-
bioRxiv - Neuroscience 2019Quote: ... Three ng of RNA was used to prepare cDNA using SMART-Seq v4 Ultra Low Input Kit (Takara). Following cDNA preparation ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... coli as a DNA template) with the Neo-selection gene by using In-Fusion HD Cloning kit (Takara), and integrated the resulting leuS-Neo DNA into genomes of the tavaborole-resistant cells by using the scarred method for insertion of a nonselectable DNA fragment recombineering protocol with Neo gene as a selectable marker49.
-
bioRxiv - Cell Biology 2019Quote: ... approximately 6 μg RNA was applied for 1st-strand cDNA synthesis using PrimeScriptTM RT Reagent Kit (TaKaRa, AK6003) with oligo (dT ...
-
bioRxiv - Genomics 2020Quote: ... cDNA was generated via PrimerScriptTM RT Reagent Kit with genomic DNA Eraser (Perfect Real Time) (TaKaRa, Beijing, China). Quantitative real-time PCR analysis (q-PCR ...
-
bioRxiv - Microbiology 2019Quote: Bacterial genomic DNA was extracted and purified using the NucleoBond AXG kit (Takara Bio, Inc., Otsu, Shiga, Japan). DNA library preparation was performed using a Rapid Barcoding sequencing kit (SQK-RBK004 ...
-
bioRxiv - Plant Biology 2019Quote: ... and converted to RNA-seq libraries using the Low input library prep kit (Clontech, Mountain View, CA, USA). Each library was diluted to 2 nM ...
-
bioRxiv - Physiology 2021Quote: Real-time qRT–PCR was performed on cDNA amplified by PrimeScript II 1st Strand cDNA Synthesis Kit (Takara) using GoTaq qPCR (Promega ...
-
bioRxiv - Genomics 2021Quote: ... according to the technical manual for PrimeScriptTM First Strand cDNA Synthesis Kit (Takara Bio Inc., Mountain View, CA). Amplification of VH and VL antibody fragments was carried out according to the standard operating procedure (GenScript ...
-
bioRxiv - Synthetic Biology 2021Quote: ... The cDNA was synthesized from 1 μg of total RNA using the PrimeScript™ RT-PCR Kit (Takara). Three technical replicates were measured for gene expression levels in each cDNA sample ...
-
bioRxiv - Biophysics 2022Quote: ... Unique clones for each COP were sub-cloned in pRH2.2 using the In-Fusion Cloning kit (Takara Bio). Sequence-verified COPs were transformed into E ...
-
bioRxiv - Biophysics 2022Quote: ... Resulting fragments were cloned into linearized FM5-mCh or FM5-GFP constructs using In-Fusion Cloning kit (Takara). The resulting plasmids were sequenced to confirm correct insertion.
-
bioRxiv - Neuroscience 2020Quote: ... #AM9937).The Brp-T2A-QF2w-Hsp70-3XP3-dsRed donor plasmid was constructed using the InFusion HD Kit (Clontech, #638910). Homology arms of ∼1 kb flanking the sgRNA cut site were cloned by PCR using template genomic DNA from the Orlando strain ...
-
bioRxiv - Molecular Biology 2021Quote: ... Three μg of total RNA was subjected to reverse transcription using PrimeScript 1st strand cDNA Synthesis Kit (Takara).
-
bioRxiv - Cancer Biology 2020Quote: ... Library construction was performed using the SMARTer® Stranded Total RNA-Seq Kit v2 – Pico Input Mammalian (Takara), and paired-end sequencing was performed on the NovaSeq 6000 instrument (Illumina ...
-
bioRxiv - Neuroscience 2020Quote: ... Plasmid DNA to generate viral vectors was prepared using the NucleoBond Xtra Maxi endotoxin free kit (Takara Bio) prior to production ...
-
bioRxiv - Neuroscience 2020Quote: cDNA libraries were made by the OHSU Massively Parallel Sequencing Shared Resource using the SMARTer RNA kit (Clontech). Library quality was assessed using a TapeStation 220 and libraries were quantified by qPCR using a KAPA Library Quantification kit ...
-
bioRxiv - Plant Biology 2021Quote: ... Total RNA was extracted using the Mini BEST Plant RNA Extraction Kit (Takara Biotech Co., Ltd., Dalian, China) and cDNA was synthesized using the one-step PrimeScript RT-PCR Kit (Takara ...
-
bioRxiv - Plant Biology 2020Quote: ... using the HindIII and XbaI sites with the In-Fusion HD Cloning Kit (Takara Bio, Otsu, Shiga, Japan). The construct was verified by sequencing and used to transform Ler by the floral dip method (Clough and Bent ...
-
bioRxiv - Plant Biology 2021Quote: ... and was reverse transcribed by the PrimeScriptTM RT reagent Kit with gDNA Eraser following the manufacturer’s instructions (TaKaRa). RT-qPCR was performed with SYBR Green mix (Bimake) ...
-
bioRxiv - Microbiology 2020Quote: ... Pseudovirus-containing supernatants were collected 48 h later and concentrated using Lenti-X™ concentrator kit (Takara, CA). Pseudovirus neutralization assay was then performed by incubation of SARS-CoV-2 pseudovirus with 5 folds serially diluted antibody with starting concentration of 1 μg/ml for 1 h at 37 °C ...
-
bioRxiv - Neuroscience 2020Quote: ... reverse transcription and amplification was achieved using the SMART-Seq v4 ultra low input RNA Kit (Takara, 634891). This kit improves synthesis of the full-length cDNA via a template switching mechanism for synthesis of the second strand cDNA ...
-
bioRxiv - Neuroscience 2020Quote: ... cDNA library preparations were conducted using the SMART-Seq v4 Ultra Low Input kit from TaKaRa (Cat. # 634894) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2019Quote: ... RNA was amplified using SMARTer® Universal Low Input RNA Kit for sequencing (Takara, Mountain View, CA, USA). Ribosomal RNA was depleted using RIboGone™-Mammalian rRNA removal kit (Takara ...
-
bioRxiv - Molecular Biology 2021Quote: ... The respective PCR products were then cloned into pcDNA5_FRT_TO_3xFlag(N) using In-Fusion® HD Cloning Kit (Cat. No. 639650, Takara).
-
bioRxiv - Microbiology 2021Quote: ... Reverse transcription was done using PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) (Takara, #RR047A). qPCR reaction was prepared by TB Green® Premix Ex Taq™ (Tli RNaseH Plus ...
-
bioRxiv - Systems Biology 2021Quote: ... Ribosomal RNA was depleted using the RiboGone – Mammalian Kit protocol (Cat #634847, Takara Bio USA, Mountain View, CA). The SMARTer Universal Low Input RNA Kit protocol v2 (Cat #634938 ...
-
bioRxiv - Microbiology 2020Quote: ... converted to single stranded cDNA and amplified using the SMARTer PCR cDNA Synthesis Kit (Takara Bio, Kusatsu, Japan), and IsoSeq libraries were constructed with equimolar cDNA fractions (0.5X and 1X ...
-
bioRxiv - Cell Biology 2021Quote: Total RNA was isolated from wild-type or transiently transfected cells with MiniBEST Universal RNA Extraction Kit (Takara), an additional DNase I (NEB ...
-
bioRxiv - Cell Biology 2020Quote: ... 100 ng of total RNA was reverse-transcribed using the PrimeScript RT Reagent Kit (Takara Bio, Shiga, Japan) and a T100 thermal cycler (Bio-Rad Laboratories ...
-
bioRxiv - Cell Biology 2021Quote: ... total RNA was extracted using TRIzol reagent (Fermentas) and reverse transcribed using the mRNA Selective PCR Kit (TaKaRa) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: RNA was quantified (by Nanodrop) and retrotranscribed to cDNA (PrimeScript™ RT-PCR Kit - Takara Bio Cat. # RR014A). Real time PCR was performed with KAPA SYBR® FAST qPCR Master Mix (2X ...
-
bioRxiv - Cell Biology 2021Quote: Total genomic DNA of HeLa cells was extracted after irradiation using Total genomic DNA extract kit (TAKARA, China). Human nuclear GAPDH gene (forward ...
-
bioRxiv - Biochemistry 2022Quote: ... AAV-GFP and AAV-Mafa were purified with the AAV pro Extraction Solution Kit (6235, Takara, Shiga, Japan), and the titers were determined with a Quick Titer AAV Quantification Kit (VPK-145 ...
-
bioRxiv - Physiology 2022Quote: ... cDNA was synthesized using 2 μg RNA using the PrimeScript™ RT reagent Kit with gDNA Eraser (TaKaRa). Gene specific primers for quantitative real-time PCR (qRT-PCR ...
-
bioRxiv - Plant Biology 2022Quote: ... and 3’ UTR region was cloned into the PacI site in pZH_OsU6sgRNA_SpCas-wt (Endo et al., 2019) using an In-Fusion HD cloning kit (Takara). Two pairs of 20-nt annealed oligonucleotides targeting exon 4 of the OsSOG1 gene were cloned into the BbsI site of the pU6gRNA-oligo (pU6::sgOsSOG1-2 and pU6::sgOsSOG1-3 ...