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Citations for Takara Bio :
3201 - 3250 of 5041 citations for QuantiChrom Arginase Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... DNA fragmentation was performed using In situ Apoptosis Detection Kit according to the manufacturer’s indications (Takara Bio Inc.). For immunostaining ...
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bioRxiv - Cell Biology 2023Quote: ... and N-SREBP2 (1440 bps) were amplified using high-fidelity PCR (Advantage-HF 2 PCR kit, #639123; Clontech) from human pcDNA3.1-2xFLAG-SREBP1a (#26801 ...
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bioRxiv - Immunology 2023Quote: ... Total RNA was normalized prior to oligo-dT capture and cDNA synthesis with SMART-seq v4 kit (Takara). The resulting cDNA was quantitated using a Qubit 3.0 fluorometer ...
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bioRxiv - Immunology 2023Quote: ... linearized BF520 sequence was gel purified using NucleoSpin Gel and PCR Clean-up kit (Takara, Cat. No. 740609.5) and then purified using Ampure XP beads (Beckman Coulter ...
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bioRxiv - Cell Biology 2023Quote: ... and a PrimeScript RTreagent kit with gDNA Eraser and SYBR Premix Ex Taq II (TliRNase H Plus) (TaKaRa) and an ABI7500 qPCR instrument (Life Technologies) ...
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bioRxiv - Microbiology 2023Quote: ... The PCR product was cloned into SalI-PstI-digested pKG120 by In-Fusion HD Cloning Kit (Takara bio). The resulting plasmid ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was prepared using the SMARTer procedure through the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech) for Illumina Sequencing ...
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bioRxiv - Plant Biology 2023Quote: ... and terminator was assembled into the backbone PCB1532 via In-Fusion cloning (In-Fusion cloning kit, Clontech Laboratories).
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bioRxiv - Neuroscience 2023Quote: ... First strand cDNA synthesis was performed on 200ng RNA using the SMARTer PCR cDNA Synthesis Kit (Clontech, UK) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... 4 μg of total RNA was used for cDNA synthesis with PrimeScript 1st strand cDNA Synthesis Kit (Takara), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Sequencing libraries from treated RNA were created using the SMARTer Stranded Total RNA HI Mammalian kit (Takara 634873) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... was performed to determine the expression level of transcripts utilizing the SYBR Green PCR master mix kit (Takara). The unique region of transcripts was selected for designing qRT-PCR primers and mentioned in Table S3 ...
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bioRxiv - Plant Biology 2024Quote: ... amplified promoter region fragments are inserted with KpnI and XhoI site in pAT006-Fluc-iG5_Rluc-iFAD2_F vector using In-Fusion HD Cloning Kit (Takara). Amplified BOT6 fragment was inserted with pGWB402 using pENTR™ Directional TOPO® Cloning Kits (Invitrogen).
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bioRxiv - Developmental Biology 2024Quote: Single-cell cDNA synthesis was performed using SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara) according to the manufacturer’s protocol with some modifications as previously described17 ...
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bioRxiv - Synthetic Biology 2024Quote: ... One µg of total RNA was processed using PrimeScript RT reagent Kit with gDNA Eraser (Takara, Beijing, China). Amplified products were sequenced and verified after cloning into a pJET1.2-T vector through CloneJET PCR Cloning Kit (Thermo Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... The pmEOS2-C1-ERGIC53 plasmid was constructed using using the In-Fusion® Snap Assembly Cloning Kit (Takara) and transformed into OmniMAX competent cells ...
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bioRxiv - Microbiology 2023Quote: ... Reverse transcription was performed to make cDNA using PrimeScript RT reagent kit with gDNA Eraser (TaKaRa, Kyoto, Japan). Sequences encoding the C-terminal portion (amino acid 45 to 131 ...
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bioRxiv - Immunology 2023Quote: ... Total RNA was normalized prior to oligo-dT capture and cDNA synthesis with SMART-seq v4 kit (Takara). The resulting cDNA was quantitated using a Qubit 3.0 fluorometer ...
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bioRxiv - Molecular Biology 2023Quote: ... The 5’ and 3’ ends of the cDNA were amplified using the SMARTer RACE cDNA Amplification Kit (Clontech) according to the manufacturer’s guidelines ...
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bioRxiv - Biochemistry 2023Quote: ... Genomic DNA from WT and Strt1−/− cells was extracted with a NucleoSpin Tissue kit (Takara Bio, Shiga, Japan) and subjected to PCR using specific primers (Oligonucleotide sets 3 ...
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bioRxiv - Biophysics 2023Quote: ... The first-strand cDNA was reverse transcribed with PrimeScript™ RT Reagent Kit with gDNA Eraser (TaKaRa, Japan). Quantitative real-time PCR was run on a Roche LightCycler® 480 II real-time PCR detection system ...
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bioRxiv - Bioengineering 2023Quote: ... The synthesized mRNA was subsequently purified using a NucleoSpin® RNA Clean-Up Kit (Takara Bio Inc., Japan). The concentration of the purified mRNA was quantified using a Nanodrop One (Thermo Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... Purified insert DNA was cloned into the linearized modRNAc1 plasmid using the In-Fusion Cloning Kit (Takara Bio). The DNA template for modRNA synthesis was PCR amplified from the successfully cloned modRNAc1 plasmid followed by PCR purification using DNA Clean & Concentrator-5 (Zymo Research) ...
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bioRxiv - Genomics 2023Quote: ... Libraries were prepared using the SMARTer Stranded Total RNA-Seq kit v2 - Pico Input Mammalian (Clontech, TaKaRa Bio). Unfragmented RNA was reverse transcribed with adapter-linked random hexamers and the first strand cDNA was then converted into double-stranded dual-indexed DNA libraries ...
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bioRxiv - Developmental Biology 2023Quote: ... 18-20 μg of indicated plasmids were transfected in HEK293T cells using the CalPhos Mammalian Transfection Kit (Takara). After 48h ...
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bioRxiv - Microbiology 2023Quote: ... 5’RACE was performed with SMARTer RACE 5’/3’ Kit (Takara Bio USA, Inc. San Jose, CA USA) according to the manufacturer’s directions.
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bioRxiv - Cancer Biology 2023Quote: ... Dox-inducible vectors were generated according to a publicly available protocol54 using In-Fusion HD Cloning Kit (Clontech). Vectors were amplified in Stellar Competent Cells (Clontech ...
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bioRxiv - Plant Biology 2024Quote: ... cDNA was prepared from 1.5 μg of total RNA using the PrimeScript 1st strand cDNA Synthesis Kit (Takara). PCR reactions were performed with 1 μl of a 1:4 dilution of cDNA in ddH2O ...
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bioRxiv - Plant Biology 2024Quote: ... The resulting samples were reverse transcribed into first-strand cDNA using a PrimeScript RT Reagent Kit (Takara Bio). Quantitative PCR (qPCR ...
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bioRxiv - Plant Biology 2024Quote: ... We then performed first-strand synthesis using the SMARTer Stranded Total RNA Kit V2- Pico Input Mammalian (Takara). Following purification with RNAclean XP (Beckman Coulter) ...
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bioRxiv - Zoology 2024Quote: ... One µg of total RNA was processed using PrimeScript RT reagent Kit with gDNA Eraser (Takara, Beijing, China). The PCR procedure was set as follows ...
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bioRxiv - Immunology 2024Quote: ... Viral titer (p24 core antigen content) was determined initially using a LentiX GoStix Plus titration kit (Takara #631280) and subsequently by HIV p24 AlphaLISA detection kit (PerkinElmer # AL291C) ...
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bioRxiv - Cell Biology 2024Quote: ... One microgram of total RNA was subjected to first strand cDNA synthesis using PrimeScript RT Reagent kit (Takara). The target cDNA was amplified using TB Green Premix Ex Taq II (Takara ...
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bioRxiv - Cell Biology 2024Quote: ... One microgram of total RNA was subjected to first strand cDNA synthesis using PrimeScript RT Reagent kit (Takara). The target cDNA was amplified using TB Green Premix Ex Taq II (Takara ...
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bioRxiv - Genomics 2024Quote: Sequencing libraries were prepared from isolated RNA using two different kits: Takara SMART-Seq v4 3′ DE (Takara) and Lexogen QuantSeq 3′ (Lexogen ...
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bioRxiv - Genetics 2024Quote: ... Ligation of the various components was undertaken using an In-Fusion cloning kit (Takara Bio-Europe, Paris, France). The ligation reaction was transformed into Stellar competent cells as per manufacturer protocols ...
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bioRxiv - Genetics 2024Quote: ... Complementary DNA (cDNA) was synthesized from total RNA using a PrimeScript RT reagent Kit (Cat. no. RR047B, TaKaRa) and was used as a template to perform qPCR in TB green-based qPCR instrument (CFX-96 ...
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bioRxiv - Neuroscience 2024Quote: ... and 3 μg of RNA was reverse transcribed using PrimeScript™ RT reagent kit (Takara, San Jose, CA), according to manufacturer’s instruction ...
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bioRxiv - Neuroscience 2024Quote: ... ds-cDNA was prepared using the SMARTer Ultra Low RNA kit for Illumina Sequencing (Takara-Clontech cat# 634936) per manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... ds-cDNA was prepared using the SMARTer Ultra Low RNA kit for Illumina Sequencing (Takara-Clontech cat# 634936) per manufacturer’s protocol ...
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bioRxiv - Genetics 2024Quote: ... 1 μg of total RNA was reversely transcribed into cDNA using the Prime Script RT kit (Takara, RR047A). The SYBR Green qPCR master mix (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... Then dual-indexing library preparation was created in equimolar conditions following the SMARTSeq Library Prep kit (Takara Bio) and Illumina sequencing 2x 150 reads was done on a Novaseq SP by Fasteris-GeneSupport SA (Switzerland) ...
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bioRxiv - Genetics 2024Quote: ... These three fragments were cloned into EcoRI-digested pBluescript-II vector using In-Fusion HD cloning kit (Takara). gRNA plasmids were constructed by cloning of annealed oligo DNA (Ti902/903 and Ti904/905 ...
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bioRxiv - Neuroscience 2024Quote: ... Viral titer (p24 core antigen content) was determined initially using a LentiX GoStix Plus titration kit (Takara #631280) and subsequently by HIV p24 AlphaLISA detection kit (PerkinElmer #AL291C) ...
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bioRxiv - Neuroscience 2024Quote: ... strand cDNA was synthetized using the TaKaRa PrimeScipt RT Reagent Kit (Cat# RR037A, Takara Bio Inc., Kusatsu, Japan) using 20 µM Random decamer first strand primers according to the manufactorer’s instructions.
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bioRxiv - Evolutionary Biology 2024Quote: ... and upstream fragment) were connected using the In-Fusion reaction (In-Fusion HD Cloning Kit, Takara, Shiga, Japan) to construct the LBDG_21500-disrupting plasmid (Table S9).
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bioRxiv - Immunology 2024Quote: ... In-fusion assembly with the 2 fragments was performed using the In-fusion snap assembly kit by Takara Bio.
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bioRxiv - Microbiology 2024Quote: ... The viral RNA copy number was standardized using a SARS-CoV-2 direct detection RT-qPCR kit (Takara). Fluorescent signals from resulting PCR products were acquired using a Thermal Cycler Dice Real Time System III (Takara).
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bioRxiv - Plant Biology 2020Quote: ... The first-strand cDNA was synthesized from genomic DNA-removed RNA by using PrimeScript™ RT reagent kit (TaKaRa). Next ...
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bioRxiv - Immunology 2021Quote: ... 1ng of total RNA was pre-amplified with the SMARTer Ultra Low Input kit v4 (Clontech, Mountain View, CA) per manufacturer’s recommendations ...