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Citations for Takara Bio :
3201 - 3250 of 5557 citations for Human E3 Ubiquitin Protein Ligase SMURF2 SMURF2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... DNA ligation was done using standard ligation techniques (Takara DNA Ligation kit ver.2.1, Takara, Otsu, Japan). For the transformation ...
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bioRxiv - Molecular Biology 2020Quote: ... The 3xFlag sequence was then ligated into the digested eGFP-Usp9x plasmid (Takara DNA ligation kit #6023).
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bioRxiv - Molecular Biology 2020Quote: The RNA-seq library in oocytes was prepared using the SMART-Seq® Stranded Kit (TaKaRa, 634442). Total RNAs obtained using NucleoSpin® RNA Plus XS (TaKaRa ...
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bioRxiv - Cell Biology 2022Quote: ... and complementary DNA of miR was synthesized using the Mir-X miRNA First-Strand Synthesis Kit (TaKaRa). RT-qPCR was performed with TB Green Premix Ex Taq II (Tli RNaseH Plus ...
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bioRxiv - Genomics 2020Quote: ... for subsequently cloned using the InFusion HD Cloning Kit protocol (Takara Bio, Saint-Germain-en-Laye, France). All plasmids were confirmed by Sanger sequencing ...
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bioRxiv - Genomics 2020Quote: ... The titer of each virus was determined using the Lenti-X Go-Stix Plus kit (Takara Bio), according to the manufacturer’s instruction.
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bioRxiv - Immunology 2021Quote: ... using One Step TB Green(tm) PrimeScript(tm) RT-PCR Kit II (SYBR Green) (RR086B, TaKaRa, JAPAN). Relative copy number was determined by calculating the fold-change difference in the gene of interest relative to GAPTH ...
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bioRxiv - Molecular Biology 2019Quote: ... apis mycelia was reverse transcribed to cDNA using the RevertAid First Strand cDNA Synthesis Kit (TaKaRa, China) and Oligo dT primers ...
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bioRxiv - Immunology 2019Quote: ... and cDNA was prepared using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara). Library construction was performed using the NexteraXT DNA sample preparation kit (Illumina ...
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bioRxiv - Developmental Biology 2019Quote: ... Library preparation followed the SMARTERÒ stranded Total RNA-Seq Kit v2 – Pico Input Mammalian (Takara Bio, Japan). Due to the degraded nature of input material we omitted the fragmentation step ...
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bioRxiv - Genetics 2019Quote: BAC clone (BAC PAC RPCI- 98 library) DNA was extracted using a MIDI-prep kit (Clontech #740410). The probe for the bw locus was Clone BACR48M01 ...
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bioRxiv - Cancer Biology 2019Quote: ... RNA-Seq libraries were prepared using the SMART-Seq v4 Ultra Low Input RNA Kit (Takara, 634889) from total RNA ...
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bioRxiv - Cancer Biology 2019Quote: ... And then total RNAs were reversely transcribed into cDNA using MLV RTase cDNA Synthesis Kit (Takara, Japan). Quantitative RT-PCR was conducted in Roche Applied Science LightCycler 480II Real-time PCR systems (Roche Applied Science ...
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Mutant ACTB mRNA 3′UTR Promotes Hepatocellular Carcinoma Development by Regulating miR-1 and miR-29abioRxiv - Cancer Biology 2019Quote: ... the isolated RNA was reverse transcribed to cDNA using a PrimeScript RT reagent Kit (Takara, Dalian China). Real-time PCR analyses were performed with SYBR Green (Takara ...
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bioRxiv - Developmental Biology 2019Quote: ... and then cDNA was synthesized using the PrimeScript reverse transcription (RT) reagent kit (6210B; Takara Bio. Inc.) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... 1 ng of total RNA was amplified using the SMARter Ultra Low amplification kit (Clontech, Mountain, CA) with PCR amplification to generate sufficient cDNA for library construction ...
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bioRxiv - Microbiology 2019Quote: ... the cDNA was purified and eluted in 20ul of elution buffer (NucleoSpin PCR Clean-up Kit, Clontech). The immunoglobulin PCRs were set up with Platinum Taq High-Fidelity DNA Polymerase (Life Technologies ...
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bioRxiv - Developmental Biology 2019Quote: ... The PCR products were extracted from agarose gel using MiniBEST Agarose Gel DNA Extraction Kit (Takara, Japan) based on manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... and cDNA synthesized from mRNA using SMART-Seq® v4 Ultra™ Low Input RNA Kit (Clontech). Library was then constructed and sequenced on Illumina HiSeq platform with the PE150 strategy ...
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bioRxiv - Molecular Biology 2020Quote: ... Then cDNA was synthesized according to the protocol of the RT-PCR kit (Takara; Kusatsu, Shiga, Japan) and used as templates for quantitative PCR ...
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bioRxiv - Plant Biology 2020Quote: ... and first-strand cDNA was synthesized using the PrimeScript II 1st strand cDNA Synthesis Kit (TAKARA Bio), according to the manufacturer’s protocol.
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bioRxiv - Genetics 2021Quote: ... and One Step TB Green™ PrimeScript™ RT-PCR Kit II (SYBR Green) (TaKaRa RR086B, Japan).
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bioRxiv - Neuroscience 2021Quote: ... total RNA was used for reverse transcription (RT) using the SMARTScribe Reverse Transcription Kit (TaKaRa, Cat #639537).
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bioRxiv - Evolutionary Biology 2021Quote: ... pre-amplified (DFRS empty plasmid primers) and pre-digested by the same enzymes (In Fusion Kit; Clontech). The miR-137 or miR-122 complementary sequences were thereby located on the 3’UTR of the RFP gene.
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bioRxiv - Neuroscience 2020Quote: ... The lentiviral genome copy number was calculated using the Clontech Lenti-X qRT-PCR Titration kit (Takara).
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bioRxiv - Developmental Biology 2020Quote: ... RNA-seq libraries were prepared with the SMART-Seq v4 Ultra Low Input RNA Kit (Takara Bio) with poly(A ...
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bioRxiv - Biochemistry 2020Quote: ... and cell toxicity was evaluated using the LDH Cytotoxicity Detection Kit (Cat #MK401; Takara Bio, Shiga, Japan).
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bioRxiv - Neuroscience 2020Quote: Cell line CUT&RUN libraries were prepared using the SMARTer ThruPLEX DNA-seq Kit (Takara Bio R400676), with the following PCR conditions ...
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bioRxiv - Cancer Biology 2019Quote: ... and subsequently subjected to reverse transcription using PrimeScript™ RT reagent Kit (TaKara Bio, Mountain View, CA). Quantitative real-time PCR was performed with Premix Ex Taq (TaKara Bio ...
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bioRxiv - Cell Biology 2021Quote: DNA insertion and site-directed mutagenesis were performed using an In-Fusion HD Cloning Kit (TaKaRa Bio) or by restriction enzyme fragmentation ...
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bioRxiv - Plant Biology 2021Quote: ... All cloning was done using In Fusion® HD Cloning kit (Takara, California, USA; Cat. No. 639649). First ...
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bioRxiv - Plant Biology 2020Quote: ... and reverse-transcribed with a random hexamer using the PrimeScript II 1st strand cDNA Synthesis kit (TaKaRa). Gene specific primers were used to amplify cDNA by PCR using Ex Taq Hot Start Version (Takara) ...
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bioRxiv - Microbiology 2020Quote: Genomic DNA was isolated from Vero cells using the NucleoSpin Tissue kit (Takara Bio Inc., Shiga, Japan). To determine the sequence of the N-terminal PVR1- and PVR2-coding regions ...
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bioRxiv - Microbiology 2020Quote: ... and ligating them into their respective vectors using In-Fusion® HD Cloning Kit (Takara Bio USA) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... DNA extraction was performed using a NucleoSpin Soil Kit (Takara Bio USA, Inc., Mountain View, CA, USA) according to the manufacturer’s instructions with the following optimizations ...
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bioRxiv - Plant Biology 2020Quote: ... First-strand cDNA was synthesized using the PrimeScriptTM II 1st Strand cDNA Synthesis Kit (TAKARA, Kusatsu, Japan) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: ... Total RNA was extracted from collected plant leaves using TaKaRa MiniBEST Universal RNA Extraction Kit (Takara, Japan). 1 ug of total RNA was reverse-transcribed into cDNA using PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time ...
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bioRxiv - Plant Biology 2021Quote: Total RNA was extracted from three-week subcultured callus using an RNAiso plus Kit (TaKaRa, http://www.takara.com.cn) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Amplified cDNA libraries were prepared using Takara’s SMART-seq v4 Ultra Low Input RNA Kit (Takara 634888) and sequenced on Illumina’s NovaSeq 6000 platform ...
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bioRxiv - Neuroscience 2020Quote: RNA-Seq libraries were prepared using SMARTer Stranded Total RNA-Seq Kit v2 – Pico Input Mammalian (Takara Bio USA ...
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bioRxiv - Neuroscience 2020Quote: ... Physical viral titer was determined using either Lenti-X qRT-PCR Titration Kit (Takara, Mountain View, CA) or qPCR Lentivirus Titration Kit (Applied Biological Materials Inc. ...
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bioRxiv - Neuroscience 2020Quote: ... The genome copy number was calculated using the Clontech Lenti-X qRT-PCR Titration kit (Takara Bio).
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bioRxiv - Neuroscience 2020Quote: ... it was inserted into the pUAST attB vector using an In-Fusion® HD Cloning Kit (Clontech). The primer sequences used for the PCR are as follows ...
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bioRxiv - Microbiology 2020Quote: ... or (ii) the SMARTer Stranded Total RNA-Seq Kit v2 – Pico Input Mammalian libraries (Takara Bio USA) for ALG_1 and ALG_3 (Table S1) ...
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bioRxiv - Neuroscience 2020Quote: ... The approximate titer of lentivirus was evaluated using a lentiviral titration kit (Lenti-X GoStix Plus; ClonTech). The lentiviral medium was transferred to 50 mL centrifuge tubes ...
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bioRxiv - Microbiology 2021Quote: ... which was prepared with the ThruPLEX DNA-seq Kit (Takara Bio USA, Inc., Mountain View, CA, USA). 2 × 250 bp sequencing was performed using the Illumina HiSeq 2500 V4 platform ...
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bioRxiv - Physiology 2021Quote: ... 1 μg RNA was used to synthesize first strand of cDNA using PrimeScriptTM RT-PCR Kit (TaKaRa). qPCR was conducted using PrimeScriptTMRT Master Mix (TaKaRa ...
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bioRxiv - Plant Biology 2021Quote: ... the RNA was converted into cDNA using PrimeScript™ II 1st Strand cDNA Synthesis Kit (TAKARA, 6210A) following the instruction in the manual ...
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bioRxiv - Plant Biology 2021Quote: ... Applied Biosystem StepOne real-time PCR system and SYBR Premix Ex Taq II kit (Takara, Dalian, China) were used for the qRT-PCR detection ...
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bioRxiv - Molecular Biology 2021Quote: ... In-Fusion cloning was performed using the In-Fusion® HD Cloning Plus Kit (Takara Bio, Japan) following the manufacturer’s protocol ...