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Citations for Takara Bio :
3001 - 3050 of 5356 citations for Pig Glucagon Like Peptide 2 GLP 2 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... we synthesized the first-strand cDNA using the PrimeScript 1st-strand cDNA synthesis kit (TaKaRa, Japan). The qRT-PCR was conducted in a 20μl reaction volume employing SYBER Green Master Mix (TaKaRa ...
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bioRxiv - Plant Biology 2023Quote: ... Site-directed mutagenesis at the CESA1 sequence was performed using the infusion cloning kit from Takara Bio ...
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bioRxiv - Plant Biology 2023Quote: Total RNA was extracted from Col-0 and mutant seedlings using an RNAiso Plus kit (Takara). Complementary DNAs (cDNA ...
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bioRxiv - Plant Biology 2023Quote: Yeast one-hybrid assays were performed using the Matchmaker Gold Yeast One-Hybrid System Kit (TaKaRa) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... the amplified DNA fragments were self-ligated using the In-Fusion HD cloning kit (TAKARA, Japan) to generate pET15-SmaI.
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bioRxiv - Immunology 2023Quote: ... and was cloned into pT2/HB vector through In-Fusion HD Cloning Plus kit (Takara Bio) following manufacturer’s instructions.
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bioRxiv - Biochemistry 2023Quote: The plasmids for producing XccOpgD mutants were constructed using a PrimeSTAR mutagenesis basal kit (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The RACE product was cloned using the In-Fusion® HD Cloning Kit (Takara Bio USA) following the producer’s instructions and modifications as described by Botella et al ...
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bioRxiv - Evolutionary Biology 2024Quote: ... cDNA was synthesized using a SMARTer RACE cDNA Amplification Kit (Clontech Laboratories, Mountain View, CA, USA). Gene-specific primers were designed using Primer3 Plus (Untergasser et al. ...
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bioRxiv - Genomics 2024Quote: ... employing the Fruit-mate™ and NucleoSpin™ RNA Plant kits (Takara Bio Inc., Shiga, Japan), in accordance with the manufacturers’ instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... paired-end library preparation was performed using the SMART-Seq HT kit (Takara, Cat. No. 634437) followed by Nextera XT DNA Library Preparation Kit (Illumina ...
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bioRxiv - Genetics 2024Quote: ... The cells were then harvested and genomic DNA was extracted using NucleoSpin Blood XL kit (Clontech). Libraries were prepared and sequenced as previously described 4.
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bioRxiv - Plant Biology 2024Quote: ... cDNA was reverse transcribed using the PrimeScriptTM RT reagent kit (Perfect Real Time) (TaKaRa, Shiga, Japan). The cDNA mixture was used for qRT-PCR using GoTaq® qPCR and RT-qPCR Systems (Promega ...
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bioRxiv - Microbiology 2024Quote: cDNA synthesis and amplification were performed using SMARTSeq V4 Ultra Low Input RNA kit (Takara Bio). Then dual-indexing library preparation was created in equimolar conditions following the SMARTSeq Library Prep kit (Takara Bio ...
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bioRxiv - Cancer Biology 2024Quote: ... 1ng of total RNA was pre-amplified with the SMARTer Ultra Low Input kit v4 (Clontech) per manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA unique Dual index kit was used to combine libraries for sequencing (Takara Bio Inc, USA). NOVASEQ 3000 was used for sequencing at the NIBMG core facility (COTERI ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA was made from 100 or 200 ng of RNA using PrimeScript RT Reagent Kit (TaKaRa). Quantitative PCR was performed using TB Green Premix Ex Taq II (TaKaRa ...
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bioRxiv - Cancer Biology 2024Quote: ... and genomic DNA amplification was carried out using the PicoPLEX Single Cell WGA kit v3 (Takara) and following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Synthesis of cDNA was performed using a PrimeScript™ RT reagent kit (TaKaRa Bio; Tokyo, Japan), and PCR was performed with Taq DNA Polymerase (Toyobo ...
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bioRxiv - Neuroscience 2024Quote: Sample preparation for SMART-Seq was performed using the SMART-Seq v4 kit (Takara Cat#634894) as described previously10 ...
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bioRxiv - Neuroscience 2024Quote: ... ds-cDNA was prepared using the SMARTer Ultra Low RNA kit for Illumina Sequencing (Takara-Clontech) per manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... ds-cDNA was prepared using the SMARTer Ultra Low RNA kit for Illumina Sequencing (Takara-Clontech) per manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... described below) were constructed via FastCloning (63) or with the In-Fusion HD Cloning Kit (Takara). Primers used in this study are listed in Table S4 ...
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bioRxiv - Molecular Biology 2024Quote: ... For in vitro experiments the AAV vectors were purified using AAVpro® Purification Kit (Takara, 6666) following manufacturer instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... according to manufacturer’s directions for the SMART-seq v4 Ultra Low Input RNA Kit (Takara, 634891) at a 1/8th reaction volume and using the Mantis (Formulatrix ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA was synthesized from 100 ng of RNA using a PrimeScript 1st strand cDNA Synthesis Kit (Takara). The resulting cDNA was quantified with a LightCycler 480 (Roche ...
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bioRxiv - Plant Biology 2019Quote: cDNA was synthesized with oligo(dT) primers using PrimeScript™ 1st strand cDNA synthesis kit (Takara, Japan). Quantitative RT-PCR analysis was performed on an Agilent Real-Time qPCR apparatus (Mx3000P system ...
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bioRxiv - Biophysics 2021Quote: ... The first-strand cDNA was synthesized using a PrimeScript® RT reagent Kit (Takara, Otsu, Shiga, Japan). The PlMYB308 gene was PCR-amplified using specific primers (Table S6) ...
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bioRxiv - Cell Biology 2020Quote: ... RNA was isolated from cells using the NucleoSpin RNA Plus kit of Clontech Laboratories (Takara Bio Company). cDNA was constructed using the In-Fusion SMARTer Directional cDNA Library Construction kit of Takara Bio ...
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bioRxiv - Cancer Biology 2021Quote: ... QPCR reactions were performed according to the manufacturer’s instructions using SYBR® Premix Ex Taq kit (TaKaRa). All reactions were performed in triplicates ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA was extracted from cells and quantified using RNA Isolation Reagent kit (9109, Takara, Tokyo, Japan). cDNA was obtained from 1 µg of total RNA from each sample (Super M-MLV reverse transcriptase ...
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bioRxiv - Cell Biology 2019Quote: ... RNA was extracted and cDNA was prepared using the SMARTer v3.0 or v4.0 cDNA synthesis kit (Clontech). RNA-Seq was performed on an Illumina Nextseq 500 machine ...
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bioRxiv - Developmental Biology 2021Quote: ... Library preparations were done using SMARTer® Stranded RNA-Seq Kit (Clontech Laboratories, Inc. #634836 or #634837) according to manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2020Quote: ... and processed for library generation using SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (TaKaRa) following the manufacturer’s recommendations ...
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bioRxiv - Immunology 2021Quote: ... and all resulting RNA was used as input for the SMART-seq v4 cDNA synthesis kit (Takara) with 12 cycles of PCR amplification ...
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bioRxiv - Cell Biology 2020Quote: ... and cDNA was synthesized using the SMART-Seq v4 Ultra Low Input RNA Kit (Takara Bio Europe). Sequencing libraries were prepared with the Nextera® XT DNA Library Preparation Kit for Illumina® ...
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bioRxiv - Genetics 2021Quote: ... Total RNA was reverse transcribed to cDNA using the PrimeScript cDNA synthesis kit (Takara Bio, Kusatsu, Japan). Quantitative PCR (qPCR ...
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bioRxiv - Immunology 2021Quote: ... polyA-selected transcriptome libraries were constructed using the SMART-seq v4 Ultra Low Input RNA Kit (Takara) combined to the Nextera XT DNA Library Preparation Kit (Illumina ...
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bioRxiv - Microbiology 2020Quote: ... Viral titer was determined by the ELISA p24 antigen assay (Lenti-X p24 Rapid Titer Kit, TaKaRa). Monocytes were infected immediately after purification by adding HIV-1BaL virus to the culture at MOI between 3 and 5 based on the p24 titer ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 ng of total RNA was used with the SMARTer Stranded Total RNA-Seq Kit v2 (Takara) in accordance with the low-throughput protocol ...
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bioRxiv - Immunology 2021Quote: ... Reverse transcription was performed with a PrimeScript RT Reagent Kit with gDNA Eraser (Takara, Cat no. RR047A) and qRT-PCR was performed on a StepOnePlus Real-time PCR system (Applied Biosystems ...
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bioRxiv - Evolutionary Biology 2021Quote: ... we ligated an RNA oligo of the SMART RACE cDNA Amplification Kit (Takara Bio Inc., Shiga, Japan) at 3-end of the total RNA during reverse transcription ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA-seq libraries were performed with SMARTer® Stranded Total RNA-Seq Kit v2 (Takara - rRNA depletion) and sequenced in 100bp PE reads run on NovaSeq 6000 (Illumina) ...
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bioRxiv - Microbiology 2022Quote: ... cDNA libraries were prepared using the SMARTer® Universal Low Input RNA Kit for Sequencing (Takara Bio), without rRNA depletion ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA synthesized from 1 μg total RNA using PrimeScript™ RT reagent Kit with gDNA Eraser (Takara). Real-time PCR was performed using TB Green® Premix Ex Taq™ II (Tli RNaseH Plus ...
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bioRxiv - Neuroscience 2021Quote: The sgRNA sequences were amplified using Guide-it™ CRISPR Genome-Wide Library PCR Kit (Takara, 632651) and subjected to the high-throughput amplicon sequencing on NextSeq500 ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA ligation was done using standard ligation techniques (Takara DNA Ligation kit ver.2.1, Takara, Otsu, Japan). For the transformation ...
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bioRxiv - Molecular Biology 2020Quote: ... The 3xFlag sequence was then ligated into the digested eGFP-Usp9x plasmid (Takara DNA ligation kit #6023).
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bioRxiv - Molecular Biology 2020Quote: The RNA-seq library in oocytes was prepared using the SMART-Seq® Stranded Kit (TaKaRa, 634442). Total RNAs obtained using NucleoSpin® RNA Plus XS (TaKaRa ...
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bioRxiv - Cell Biology 2022Quote: ... and complementary DNA of miR was synthesized using the Mir-X miRNA First-Strand Synthesis Kit (TaKaRa). RT-qPCR was performed with TB Green Premix Ex Taq II (Tli RNaseH Plus ...