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Citations for Takara Bio :
2951 - 3000 of 5041 citations for QuantiChrom Nitric Oxide Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Libraries were prepared at La Jolla Institute for Immunology using SMARTer Stranded RNA-Seq kit (Takara 634962) and were sequenced using an Illumina HiSeq2500 in Rapid Run Mode with paired end 50 reads.
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bioRxiv - Cancer Biology 2023Quote: ... Reverse transcription of RNA was carried out using the PrimeScript RT reagent Kit with gDNAEraser from TAKARA. The resulting cDNA from reverse transcription was then amplified by PCR and digested using PstI ...
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bioRxiv - Evolutionary Biology 2023Quote: The SMARTSeq HT Ultra Low Input Kit was used for full-length cDNA synthesis and amplification (Clontech), and Illumina Nextera XT library was used for sequencing library preparation ...
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bioRxiv - Microbiology 2023Quote: ... DNA in the supernatant was purified using the NucleoSpin Gel & PCR kit (Takara Bio, Kusatsu, Shiga, Japan). Sequencing libraries were prepared from 3 ng DNA with the Kapa HyperPrep Kit (Roche ...
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bioRxiv - Cell Biology 2023Quote: ... the RNA was reverse transcribed using the PrimeScriptII 1st strand cDNA Synthesis Kit (Takara bio, Shiga, Japan). Quantitative RT-PCR was performed using SYBR Premix EX TaqII (Takara bio ...
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bioRxiv - Cancer Biology 2023Quote: These RNA samples were used for cDNA synthesis using PrimeScript 1st strand cDNA synthesis kit (Takara, Japan) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... All cloning experiments were performed using an In-Fusion HD Cloning Kit (Takara Bio Inc., Shiga, Japan), and plasmid sequences were confirmed by DNA sequencing (at the Core Instrumentation Facility ...
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bioRxiv - Cell Biology 2024Quote: ... These fragments were cloned into pHAGE-EF1a-Cre-w41 plasmid using an infusion cloning kit (Takara, 638947). Lentiviral vectors were transfected into the packaging HEK293 cell line ...
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bioRxiv - Cell Biology 2024Quote: ... we amplified EF1a promoter region from pEP4 E02S CK2M EN2L by PCR and cloned into pHR_SFFV_LaG17_synNotch_TetRVP64 using infusion cloning kit (Takara, 638947). After packaging the lentivirus ...
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bioRxiv - Genetics 2024Quote: ... cDNA was generated from 1μg of total RNA using the PrimeScriptTM RT reagent kit (TaKaRa, Shiga, Japan) for the quantitation of rRNA by quantitative real-time PCR ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... cDNA was reverse transcribed by using a PrimeScript RT Reagent Kit Perfect Real Time (TaKaRa Bio Inc.). Real-time PCR was performed using a 7500 Fast Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Plant Biology 2024Quote: ... Total RNA (1 μg) was reverse transcribed into cDNA using PrimeScript RT reagent kit (Takara Bio, Japan), and expression was quantified using the TB Green® Premix Ex Taq™ II (Takara) ...
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bioRxiv - Cell Biology 2024Quote: Paraffin sections were used for H/E staining and ALP staining with a commercial staining kit (TaKaRa, TRACP & ALP double-stain kit ...
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bioRxiv - Genetics 2024Quote: ... Complementary DNA (cDNA) was synthesized from total RNA using a PrimeScript RT reagent Kit (Cat#RR047B, TaKaRa) and was used as a template to perform qPCR in TB green-based qPCR instrument (CFX-96 ...
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bioRxiv - Biophysics 2024Quote: ... and cDNA was synthesized using PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) (Takara) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... and cDNA was synthesized from 1 μg RNA using PrimeScriptTM RT Master Mix kit (Takara, Cat#RR036A) following the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2024Quote: ... the first strand cDNA was prepared using PrimeScript II 1st Strand cDNA Synthesis Kit (Takara, Beijing, China).
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bioRxiv - Cell Biology 2024Quote: ... Quantitative real-time polymerase chain reaction (qPCR) was performed using TB Green Premix Ex Taq Kit (Takara) on QuantStudio 6 Flex Real-Time PCR System (Life Technologies) ...
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bioRxiv - Bioengineering 2024Quote: ... RNA was isolated through a NucleoSpin® RNA/Protein kit (Takara Bio USA Inc. San Jose, CA) while heart issue was processed with a RNeasy Fibrous Mini kit (Qiagen ...
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bioRxiv - Bioengineering 2024Quote: ... We confirmed the cells were mycoplasma-negative using a Cycleave PCR Mycoplasma Detection Kit (Takara Bio, CY232).
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bioRxiv - Microbiology 2024Quote: The processed DNA was ready for Illumina library preparation using a SMART ChIP-seq kit (Takara, #634865) following the manufacturer’s protocol (Fig ...
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bioRxiv - Microbiology 2024Quote: ... 5′ RACE was carried out using SMARTer RACE 5′/3′ Kit (Takara Bio USA, Mountain View, CA) to identify the transcription start site (TSS ...
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bioRxiv - Neuroscience 2024Quote: ... immediately followed by reverse-transcriptions and cDNA amplification with the smart-seq HT kit (Cat# 634437, Takara). The post-amplified cDNAs (1 ng per sample ...
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bioRxiv - Molecular Biology 2024Quote: ... The digested backbone and the two amplicons were joined by In-Fusion® HD Cloning Kit (Takara). The SFFV U3 in the pAS.SFFV.d2GFP.PRE plasmid was then exchanged for other U3 sequences by digesting the plasmid with XcmI and AscI (NEB ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA sequencing libraries were prepared using SMARTer Stranded Total RNA-Seq Kit v3 (Takara Bio USA, Inc) following the recommended protocol with minor adjustments ...
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bioRxiv - Cell Biology 2024Quote: ... The lentiviral titer was then determined using the Lenti-X p24 rapid titer kit (Takara Cat #632200). The Tau-RD-P301S-K18 FRET Biosensor CRL-3275 were exposed to active recombinant P301S-MAPT fibrils (ab246003 ...
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bioRxiv - Synthetic Biology 2021Quote: ... DmrA and DmrC were amplified and then fused to each split-Cas9 (N_Cas9 and C_Cas9) using the In-Fusion HD Cloning Kit (Clontech). Links to sequences of all the generated plasmids are listed in Supplementary Table 3.
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bioRxiv - Cancer Biology 2021Quote: Full-length cDNA was synthesized from total RNA (1 μg) by SMARTer®□ PCR cDNA Synthesis kit (Clontech) according to the manufacture’s instruction ...
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bioRxiv - Cancer Biology 2021Quote: Illumina sequencing libraries of isolated RPFs was performed with the SMARTer smRNA-Seq Kit for Illumina (Takara Bio). Library preparation was performed according to the manufacturer’s protocol with the following parameters ...
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bioRxiv - Cell Biology 2020Quote: ... and reverse transcribed into cDNA using reagents from a Prime Script II first strand cDNA synthesis kit (Takara). Real-time PCR was performed with the reverse transcription products ...
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bioRxiv - Cell Biology 2020Quote: ... Libraries were prepared from 8mL of RNA using the SMARTer Stranded Total RNA-seq Pico kit v2 (Clontech), per manufacturer instructions ...
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bioRxiv - Cell Biology 2020Quote: ... 500 ng total RNA was reverse-transcribed to cDNA (Prime-Script RT reagent kit, Takara Bio, Dalian, China). The parameters set for reverse transcription were as follows ...
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bioRxiv - Genetics 2021Quote: ... Strand specific RNA-seq libraries were then constructed using the SMARTer Stranded RNA-Seq HT Kit (Clontech #634839), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Viral RNA was amplified using PrimeScript II High Fidelity One Step RT-PCR Kit (Takara Bio, Shiga, Japan) under the following reaction conditions ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was generated following the SMARTer® 5’/3’ RACE kit protocol (Takara Bio, Kusatsu, Shiga Prefecture, Japan).
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bioRxiv - Developmental Biology 2021Quote: ... cDNA synthesis was completed by SMART-Seq™ v4 Ultra™ Low Input RNA kit for sequencing (Clontech). Samples were sequenced on Illumina machines ...
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bioRxiv - Developmental Biology 2020Quote: ... Total RNA was converted to cDNA using PrimeScript RT reagent Kit with gDNA Eraser (RR047A, Takara, Dalian, China). The mRNA expression levels of the Ccl9 ...
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bioRxiv - Developmental Biology 2020Quote: Total RNA was extracted from cells using the NucleoSpin RNA II kit (Clontech Laboratories, Mountain View, CA, USA). The quality and quantity of total RNA were measured by Nanodrop 1000 (Thermo Fisher Scientific) ...
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bioRxiv - Synthetic Biology 2022Quote: Genomic DNA was extracted from sorted T cells using the Macherey-Nagel NucleoSpin Tissue XS kit (Takara #740901.250). DNA encoding the CAR costimulatory domain was amplified from the extracted genomic DNA using the forward primer 5’-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGNNNNNACTGGTTATCACCCTTTA CTGC-3’ (Integrated DNA Technologies ...
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bioRxiv - Plant Biology 2022Quote: Total RNAs were extracted from various tissues using the MiniBEST Plant RNA Extraction Kit (Takara Bio, Beijing, China). First-strand cDNAs were synthesized using TransScript One-Step gDNA Removal and cDNA Synthesis SuperMix (TransGen Biotech ...
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bioRxiv - Molecular Biology 2022Quote: The RNA samples extracted from the fruit flesh were reverse transcribed using the PrimeScript RT-PCR Kit (TaKaRa) and the oligo(dt)20 primer ...
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bioRxiv - Microbiology 2022Quote: ... The first-strand cDNA was synthesized using reverse transcriptase from the PrimeScript RT reagent kit (Takara Biotechnology, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... were purchased from Biomatik and cloned into BstBI-digested PBRM1-TetO-FUW using the ligation-free In-Fusion HD cloning kit (Takara). The construct was packaged into lentivirus using HEK293T cells and delivered into target cells together with pLenti CMV rtTA3 Hygro (w785-1 ...
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bioRxiv - Neuroscience 2021Quote: RNA from FACS-sorted cells ranging from 3,000 – 10,000 were isolated with the NucleoSpin RNA XS kit (Clontech) with on column DNase digestion according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... cells and medium were collected and AAV particles were purified by AAVpro Purification Kit (Takara Bio; Shiga, Japan) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2020Quote: ... The cDNAs used for RT-PCR were synthesized using PrimeScript First-Strand cDNA Synthesis Kits (Takara, Osaka, Japan). RT-PCR cycling conditions were as follows ...
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bioRxiv - Molecular Biology 2020Quote: ... and one μg RNA was converted to cDNA by using the PrimeScript™ RT-PCR Kit (Takara, Japan) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... then into pShuttle-CMV with EcoRV using the In-Fusion HD Cloning Plus System kit (Takara Bio USA). These were linearized with Pme1 and cotransformed with pAdEasy-1 into BJ5183 cells ...
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bioRxiv - Cell Biology 2022Quote: cDNA was generated from single cells in the 96-well plate using the SmartSeq v4 kits (Takara Bio) using 1/4th volume reactions dispensed using a Mantis dispenser (Formulatrix) ...
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bioRxiv - Immunology 2021Quote: ... cDNA was generated from polyadenylated transcripts employing the SMART-Seq v4 ultra low input RNA kit (Takara Bio). RNA and cDNA quality was analysed on a 2100 Bioanalyser (Agilent) ...