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Citations for Takara Bio :
251 - 300 of 6177 citations for Rat T Cell Surface Glycoprotein CD8 Beta Chain CD8B ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... cDNA was amplified with V3d(T)24 and R2SP primers and Terra PCR Direct Polymerase (Clontech) for 16 cycles ...
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bioRxiv - Cell Biology 2021Quote: ... HUVECs were maintained in Endothelial Cell Basal Medium 2 supplemented with Endothelial Cell Growth Medium kits (C22211, C22111, Takara). For replating cells ...
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bioRxiv - Biophysics 2023Quote: ... All remaining unsorted cells in addition to the sorted cell populations were then miniprepped (Takara NucleoSpin Plasmid miniprep kit), and DNA was stored at 4 °C short-term or at −20 °C for long-term storage.
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bioRxiv - Developmental Biology 2020Quote: ... tetracycline-controlled transactivator (tTA) and the SV40 large T antigen polyadenylation signal (Takara Bio, Mountain View, CA), into B6SJLF1 fertilized eggs ...
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bioRxiv - Cell Biology 2023Quote: ... Products with 3’-dA overhangs were cloned into T-Vector pMD19 (Simple) (Takara Bio Inc., Shiga, Japan) to use as a template for sequencing.
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bioRxiv - Synthetic Biology 2023Quote: ... and the PpERV2 cassette (PPDC1-PpERV2-Tsynth19) were ligated in tandem with pMD18-T (Takara, Beijing, China) to construct LHZ1016 ...
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bioRxiv - Immunology 2021Quote: ... cell death was assayed using a lactate dehydrogenase release assay kit (Takara BioProduct) according to manufacturer’s instructions.
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Activation of endoplasmic reticulum stress via clustering of the inner nuclear membrane protein SUN2bioRxiv - Cell Biology 2022Quote: Total RNA was extracted from cells using the NucleoSpin RNA Kit (Takara Bio) according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2022Quote: For cDNA synthesis the SMART-Seq® Single Cell Kit (634472, Takara BIO) at 1/10 of the original reaction volume was used (down-scaled SMART-Seq® workflow) ...
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bioRxiv - Immunology 2023Quote: RNA was isolated from cell pellets using Nucleospin RNA Plus XS kit (Takara), and first strand cDNA synthesis was performed using Superscript IV VILO Master Mix with ezDNAse (Thermo Fisher ...
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bioRxiv - Physiology 2023Quote: ... Titering was performed on Ad293 cells using Adeno-X Rapid Titer kit (Clontech) according to the instructions of the manufacturer 28.
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bioRxiv - Microbiology 2023Quote: ... and MS4 (feline B-cell lymphoma) (73) using an RNAiso Plus kit (Takara), in accordance with the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: Total RNA was extracted from cells by TRIzol and an RNA extraction kit (MiniBEST Universal RNA Extraction Kit, RR820A, Takara, China). Total RNA (1 μg ...
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bioRxiv - Molecular Biology 2023Quote: ... The viral particles were purified from transfected cells and titrated using the AAVpro Purification Kit Maxi and AAVpro Titration Kit (Takara Bio) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: Cell lysis was assessed by measuring Lactate Dehydrogenase (LDH) activity in cell supernatants using the LDH cytotoxicity detection kit (TaKaRa). To obtain the positive control ...
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bioRxiv - Cancer Biology 2020Quote: Whole Genome Amplification (WGA) was performed on 300 single cells for each Time by means of SMARTer PicoPLEX Single Cell WGA kit (Takara) following manufacturer’s protocol as previously described24 ...
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bioRxiv - Molecular Biology 2019Quote: Total RNA samples were isolated from the rat liver using RNAiso Plus (D9109; TaKaRa, Dalian,China). RNA samples were converted to cDNA using a RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Ank only) rat Shank3 with a C-terminal mRFP-tag were generated in pmRFP-N3 (Clontech). A construct coding for N-terminally GFP-tagged full-length rat Shank3 in the pHAGE vector was obtained from Alex Shcheglovitov (Univ ...
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bioRxiv - Neuroscience 2021Quote: ... rat Txn1 cDNAs were amplified by PCR using LA-Taq polymerase (Takara Bio Inc, Shiga, Japan) with FW-primer ...
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bioRxiv - Microbiology 2022Quote: ... Virus production was quantified by determining the amount of the Gag p24 protein using an enzyme-linked immunosorbent (ELISA) assay (Innogenetics or TaKaRa). For production of HIV-1 Env-pseudotyped viruses in Jurkat cells ...
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bioRxiv - Genomics 2023Quote: ... Primary antibodies were diluted in 5% milk in PBS-T + 0.01% sodium azide (1:2,000 for mouse anti-GFP (Clontech) and mouse anti-3V5 (Invitrogen) ...
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bioRxiv - Developmental Biology 2024Quote: ... and the PCR fragments from the mutant animals were ligated into a pMD19-T vector (TaKaRa, Osaka, Japan) in preparation for sequencing ...
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bioRxiv - Molecular Biology 2020Quote: ... the genome of Pv11 cells and the clonal cell lines were extracted with a NucleoSpin Tissue kit (Takara Bio, Shiga, Japan) and subjected to PCR using the following primer set ...
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bioRxiv - Immunology 2019Quote: ... Total RNA was isolated from the cells using an RNA Extraction Kit (Takara, Japan) and then reverse-transcribed to cDNA using the PrimeScript RT Master Mix Kit (Takara ...
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bioRxiv - Microbiology 2019Quote: ... Genomic DNA was purified from Ax2 cells with the NucleoSpin Tissue kit (Takara Bio). The following plasmids were gifted by Dr ...
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bioRxiv - Biochemistry 2020Quote: ... the cells were harvested for analysis using Guide-it™ sgRNA Screening Kit (TaKaRa). Stable I2PP2A knockdown CRISPR I2PP2A HEK293 cells were also maintained in MEM with 10% FBS supplemented with Gentamycin (50 mg/ml).
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bioRxiv - Microbiology 2020Quote: RNA was extracted from Vero cells using the NucleoSpin RNA kit (Takara Bio Inc.), and cDNA was synthesized with the PrimeScript 1st strand cDNA Synthesis Kit (Takara Bio Inc.) ...
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bioRxiv - Immunology 2021Quote: ... Target cell death was quantified with an LDH (lactate dehydrogenase) cytotoxicity assay kit (Clontech) using the manufacturer’s recommended protocol ...
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bioRxiv - Microbiology 2022Quote: ... total RNA was isolated from cells using Trizol RNA isolation kit (Takara, Shiga, Japan) and MDR1 and GAPDH transcripts were detected using a TransScript Green One-Step qRT-PCR SuperMix (TransGen Biotech ...
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bioRxiv - Cell Biology 2022Quote: ... coli DH5⊡ competent cells and isolated using a midi-prep kit (Takara Bio, 740420.10), and validated by gel electrophoresis.
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bioRxiv - Genetics 2019Quote: Viral RNA was extracted from cell suspensions using a Viral RNA Extraction Kit (TaKaRa) and following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: NK cell activity was quantified using the LDH Cytotoxicity Detection Kit (MK401, TaKaRa, Japan) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... RNA was extracted from cells using the NucleoSpin® RNA kit (Cat# 740955.50, Takara) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... as well as from various feline cell lines using an RNAiso Plus kit (Takara) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Pgcl2 and Pgcl3 cDNAs in male rat livers were amplified by PCR (PrimeSTAR DNA Polymerase Takara, Japan). The oligonucleotide primer sequences employed for this purpose are shown in Supplementary Table S1 ...
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bioRxiv - Molecular Biology 2022Quote: cDNA was prepared from EV RNA using the Single Cell RNA kit SMART-Seq_V4 Ultra Low Input RNA Kit (634890, Takara, Mountain View, CA, USA) according to manufacturer instructions using 1ng input ...
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bioRxiv - Molecular Biology 2019Quote: ... The fragment was excised from the pMD18-T vector and ligated into the pLVSIN CMV Puro destination vector (TaKaRa) resulting in the pLVSIN-miR-378/486 vector ...
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bioRxiv - Biochemistry 2020Quote: ... mGFP was fused to the N terminus of bovine Gα1 (a kind gift from T. Haga of Gakushuin University) 27 in the pEGFP-C1 vector (Clontech), after the removal of the cDNA region encoding EGFP ...
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bioRxiv - Microbiology 2019Quote: ... 41). The retroviral vector LHSXN (a gift from T. Zang and P. Bieniasz) is a derivative of LHCX (Clontech) modified to contain the following MCS 5’-AAG CTT GGC CGA GAG GGC CGA AAA CGT TCG CGG CCG CGG CCT CTC TGG CCG TTA AC-3’ between the HindIII and HpaI sites (highlighted in italics ...
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bioRxiv - Plant Biology 2019Quote: ... For Yeast Two-Hybrid assays RIPa was sucloned from the pGEM-T easy vector into pGADT7 plasmid (Clontech Laboratories) using the mentioned restriction sites ...
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bioRxiv - Plant Biology 2023Quote: ... T-DNA insertions were then analysed using specific primers (Table S1) in PCR reactions with Emerald Master Mix (Takara). PCR conditions were as follows ...
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bioRxiv - Plant Biology 2023Quote: The full length cDNA of CLC2 was firstly cloned into a pMDTM20 T-vector (Takara Bio, San Jose, CA). The CLC2 containing AIM1 deleteion and AIM2 mutation as well as LDS mutated ATG8h were generated respectively by a Mut Express® II Fast Mutagenesis Kit V2 (Vazyme ...
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bioRxiv - Plant Biology 2024Quote: ... the AtTPT gene was first amplified by PCR and then introduced into the pMD19-T vector (TaKaRa, Dalian, China) to construct pMD19-T-AtTPT ...
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bioRxiv - Molecular Biology 2021Quote: Plasmids were extracted from yeast cells by Easy Yeast Plasmid Isolation Kit (#630467, Takara Bio) and transformed into E ...
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bioRxiv - Cancer Biology 2022Quote: SV40-MES-13 cells were lysed with xTractor Buffer Kit (CLONTECH Laboratories, Inc., CA, USA). Protein specimens were quantified through BCA method and electrophoresed through 10% SDS-PAGE gels ...
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bioRxiv - Bioengineering 2020Quote: ... and the DNA isolated from the cell lysates using viral DNA extraction kit (TaKaRa, Japan) was used to determine the cleavage activity of HSV-1 genomes by Sanger sequencing and TIDE analysis ...
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bioRxiv - Microbiology 2020Quote: Total RNA was isolated from A549 cells using a CellAmp Direct RNA Prep Kit (TaKaRa), as well as murine pharyngeal and lung tissues using an RNeasy Fibrous Tissue Mini Kit (QIAGEN) ...
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bioRxiv - Neuroscience 2020Quote: ... The LDH level was measured by cell counting using an LDH cytotoxicity detection kit (Takara).
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bioRxiv - Molecular Biology 2022Quote: Single-cell K562 libraries were prepared using the SMART-seq Stranded (SSS) Kit (Takara Bio), following the manufacturer instructions with modifications ...
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bioRxiv - Neuroscience 2023Quote: Small scale AAV cell-lysates were produced using the AAVpro Purification Kit (All Serotypes) (Takara) with slight modifications ...