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Citations for Takara Bio :
251 - 270 of 270 citations for Lupatadine fumarate EP Impurity C since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... All opsins were fused to mRuby2 at their C-terminus and sub-cloned into the pCAGGS expression vector by In-Fusion cloning (Clontech, Mountain View, CA). In order to target the opsins to the soma and proximal dendrites of neurons ...
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bioRxiv - Cancer Biology 2021Quote: All tissues from mice were quick-frozen in liquid nitrogen and stored at −80°C until they were dissolved with Trizol (TaKaRa, Guangzhou, China, A161050A). RNA extraction was performed according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2020Quote: ... for 1 h at 4 °C with rotation and then transferred to gravity columns (Talon® 2 ml Disposable Gravity Column, Clontech, #635606-CLI). The lysate was drained and the beads were washed five times with cold wash buffer (50 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: ... Synthesized gene fragments went through cloning process after overnight digestion with restriction enzymes (Nde I, Xho I) at 37 °C and ligation (T4 DNA Ligase, Takara Bio, Shiga, Japan) into pET21 vector (Novagen ...
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bioRxiv - Biochemistry 2021Quote: ... After sonication and centrifugation (20 000 g, 1 h, 4 °C) the supernatant was mixed with Talon Metal Affinity Resin (Takara Bio USA, Inc.). After 1 hour incubation at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... or the BamHI/MluI site of pWPI-ACE2-zeo (for ACE2 expression plasmids)43 with 3×FLAG-tag at the C-terminus using In-Fusion® HD Cloning Kit (Takara, Cat# Z9650N). Nucleotide sequences were determined by DNA sequencing services (Eurofins) ...
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bioRxiv - Molecular Biology 2022Quote: ... Each sample was then incubated at 37°C for 30 min with 20 U of mRNA-Interferase™ MazF enzyme (Takara, Cat# 2415A) in a 10 μL volume reaction containing 1X MazF reaction buffer ...
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bioRxiv - Cell Biology 2024Quote: ... A bicistronic construct expressing human EPAC1b with a C-terminal His10 tag and SUMO3(Q89K) was constructed using the pIRES2-EGFP vector (Clontech Catalog no. 632435). The EPAC1-His10-IRES-SUMO3(Q89K ...
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bioRxiv - Molecular Biology 2023Quote: ... and 5 μg of total RNAs were reverse-transcribed into cDNA at 42°C for 1 h in 20 μl reaction mixture containing mouse Moloney leukemia virus reverse transcriptase (PrimeScript, TAKARA BIO, Shiga, Japan) with random 6 primers (TAKARA BIO) ...
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bioRxiv - Cell Biology 2023Quote: We used an hESC line that transiently expresses the C-terminal region (catalytic domain) of histone demethylase JMJD3 in a doxycycline (DOX, Takara Bio Inc, Japan) inducible manner (12 ...
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bioRxiv - Microbiology 2024Quote: ... and was then incubated with 2 mL TBS pH 8.0 pre-equilibrated Talon® Metal Affinity resin during 2 h at 4°C in a vertical rotating mixer (Takara Bio, Kusatsu, Japan). The resin was washed with 10 volumes of TBS pH 8.0 buffer containing 0.1% Triton X-100 and 10 mM imidazol (both from Sigma) ...
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bioRxiv - Cell Biology 2024Quote: ... Total RNA was extracted from the yeast cells grown at 25°C in YPD medium and mRNA was purified using the Oligotex TM-dT30 mRNA Purification Kit (Takara Bio Inc., Japan). Biotinylated cRNA was prepared from 500 ng of mRNA according to the standard Affymetrix protocol ...
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bioRxiv - Cell Biology 2024Quote: ... cells expressing FKBP-tagged proteins and FRB T2098L-MitoTrap constructs were treated with rapalog AP21967 (A/C Heterodimerizer, TaKaRa Bio, 635056 or 635057) at a final concentration of 5 µM in media ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were blocked as above and then incubated overnight at 4 °C with appropriate antibodies to GFP (1:1000 dilution; Clontech, Catalog No. 1. 632381) and tubulin α (1:2000 dilution ...
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bioRxiv - Neuroscience 2023Quote: ... Overnight primary antibody incubation was done at 4°C with one or more of the following antibodies in blocking buffer: rabbit anti-DsRed (Takara Bio, 632496, 1:500), goat anti-mCherry (Sicgen ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA sequences of PAK1 mutants with a C-terminus Flag tag were synthesized at BGI Genomics (Beijing, China) then cloned into pLVX-IRES-Neo vector (632184; Takara Bio, Kusatsu, Shiga, Japan). All sequences are in Extended Data Table 2.
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bioRxiv - Molecular Biology 2024Quote: ... EYFP-Mito (EYFP fused to mitochondrial targeting sequence of cytochrome C oxidase subunit VIII) and EYFP-ER (EYFP fused to Calreticulin ER-targeting sequence) were from Clontech (Takara Bio, Kusatsu, Japan); Vector for CRISPR/Cas9-mediated knock-out of BCL-2 was plentiCTRSPRv2 (Addgene #52961 ...
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bioRxiv - Developmental Biology 2021Quote: ... Sections were blocked in 5% goat serum then incubated with primary antibody at 4°C overnight (anti-dsRed for tdTomato (632496, 1:1000; Takara Bio, Mountain View, CA, USA), anti-SP7 (ab22552 ...
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bioRxiv - Microbiology 2022Quote: ... The amplified fragments were then fused with SmaI-linearized pUt-C-EGFP10 using the In-Fusion HD Cloning Kit (Clontech Laboratories, Mountain View, CA, USA). Gene sequences fused with the egfp gene were positioned in tandem between the linker sequences ...
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bioRxiv - Neuroscience 2024Quote: ... was performed with 500-1000 ng of RNA at 37 °C for 15 min followed by heat inactivation at 85 °C for 5 sec using the PrimeScript™ RT Reagent Kit (Takara Bio, Kusatsu, Shiga, Japan) following the manufacturer’s instructions.