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Citations for Takara Bio :
251 - 300 of 828 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... according to the manufacturer’s instructions and in combination with Fruit-mate for RNA Purification solution (Takara Bio Europe SAS ...
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bioRxiv - Neuroscience 2021Quote: ... The cDNA solution was subjected to RT-qPCR using TB Green Premix Ex Taq (Takara, rr420a) and prime script rt master mix (Takara ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were added to the blocking solution (Rabbit anti-DsRed 1:1000, stock #632496, Takara Bio ...
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bioRxiv - Neuroscience 2023Quote: ... each pipette was filled with 3μl of pipette solution which consisted of 5% RNase inhibitor (Takara) in RNase-free PBS (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: ... 1.8 μl reaction stop solution containing 5.3% SDS and 6.6 mg/mL proteinase K (9034, Takara) was added and the sample was incubated for 60 min at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... The solution was treated with 5 μg/mL RNaseA and 70 unit/ml DNase I (Takara) for 1 h at 37°C ...
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bioRxiv - Bioengineering 2023Quote: ... The solution was replaced with Laminin 511 E8 fragment (Imatrix511, Catalog #T304, TAKARA BIO, Kusatsu, Japan) solution (30 µL in 1 mL of PBS ...
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bioRxiv - Evolutionary Biology 2022Quote: ... RNA extraction with TRIZOL reagent and a high salt solution for precipitation (plant) (Takara Bio, Japan) was conducted according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The resulting solution was applied to 0.5 mL of TALON Metal Affinity Resin (#635501; Takara Bio), washed with Equilibration/Wash buffer ...
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bioRxiv - Plant Biology 2021Quote: ... RNA was extracted from five samples using the Trizol method (Takara, Japan) with the addition of RNAiso-mate to remove polysaccharides and polyphenol substances ...
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bioRxiv - Neuroscience 2021Quote: ... cDNA samples were diluted and mixed with SYBR green master mix (Takara) before loading as technical triplicates for qPCR on an Applied Biosystems 7500 ...
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bioRxiv - Plant Biology 2020Quote: ... RNA samples were treated with DNase I (RNase-free) (Takara-cat # 2270B) to remove residual genomic DNA ...
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bioRxiv - Synthetic Biology 2023Quote: ... The RNA sample was then subjected to DNase I treatment (Takara #2270B) to remove DNA and purified again using ISOSPIN Cell & Tissue RNA Kit (Nippon Gene #314-08211) ...
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bioRxiv - Microbiology 2023Quote: ... The DNA samples (10 μg each) were digested with EcoRI (Takara Bio) and SalI (TOYOBO ...
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bioRxiv - Biochemistry 2023Quote: ... DNA samples were digested with SacI and S1 nuclease (Takara Bio, #2410) and analyzed by agarose gel electrophoresis followed by staining with SYBR Gold nucleic acid gel stain (Thermo Fisher Scientific ...
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Pyruvate kinase M2 regulates Japanese encephalitis virus replication by interacting with NS1 proteinbioRxiv - Microbiology 2024Quote: ... Pre-incubated samples were loaded onto the Capturem protein A columns (TaKaRa) and centrifuged at 1000g for 1 minute at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: ... samples were heat-shock transformed into stellar competent cells (Takara Bio, Kusatsu). SOC outgrowth was performed for 1 hour at 37°C ...
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bioRxiv - Genetics 2022Quote: ... DNA samples were then amplified with PrimeSTAR GXL DNA Polymerase (Takara Bio) with PCR forward/reverse primers containing Illumina adapter sequences (Table S3 ...
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bioRxiv - Microbiology 2023Quote: ... RNA samples were reverse-transcribed using Prime Script RT Reagent kit (TaKaRa) in combination with an AS1-specific tagged primer (no ...
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bioRxiv - Neuroscience 2024Quote: ... Total RNA was isolated from samples using the RNAiso Plus (TAKARA, Japan). Detailed protocols were described in Supplementary Methods ...
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bioRxiv - Biophysics 2024Quote: ... Total RNA was extracted from the sample using Trizol(Takara, RNAiso Plus) according to the user manual ...
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bioRxiv - Immunology 2020Quote: Levels of p24 antigen in the purified SARS-CoV-2 pseudotype solution was measured by ELISA (Takara). Mouse sera were heat inactivated ...
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bioRxiv - Synthetic Biology 2022Quote: ... After removal of the blocking solution 100 μL of anti-GFP (Living Colors GFP monoclonal antibody, Clontech) diluted 1:10,000 in antibody solution (1x PBS ...
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bioRxiv - Biochemistry 2024Quote: ... AAV virus was extracted from the cells using the AAVpro® Extraction Solution kit (Catalog# 6235, Takara). Fourteen-month-old mice (n=30 ...
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bioRxiv - Immunology 2021Quote: ... directly into 1.7 ml eppendorf tubes containing 12.0 μl 1X reaction buffer (Takara) for cDNA synthesis using the SMART-Seq v4 Ultra low Input RNA kit (Takara ...
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bioRxiv - Synthetic Biology 2021Quote: ... and was washed and eluted using His60 or HisTalon Buffer Set (Takara Bio) according to the supplier’s protocol ...
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bioRxiv - Plant Biology 2021Quote: ... Each PCR was conducted using TaKaRa LA Taq® with GC buffer (TaKaRa), according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... PCR amplification was conducted using TaKaRa LA Taq® with GC buffer (TaKaRa), gene specific primers (300 nM of each) ...
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bioRxiv - Plant Biology 2022Quote: ... the membranes were stripped for 15 minutes with Western BLoT Stripping Buffer (Takara) following the manufacturer’s instructions and reprobed with Ubiquitin11 (Agrisera AS08307 ...
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bioRxiv - Developmental Biology 2021Quote: ... and homogenization (NIM2 buffer supplemented with Recombinant RNase Inhibitor (0.4 U/μL, Takara), SUPERase in (0.2 U/μL ...
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bioRxiv - Microbiology 2021Quote: ... The reaction was terminated by adding 10× loading buffer (Takara, Otsu, Shinga, Japan) and analyzed by electrophoresis on a 1% agarose gel and stained with ethidium bromide.
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bioRxiv - Genetics 2020Quote: ... 5 μl of 10X ExTaq buffer and 0.375 μl of ExTaq polymerase (Takara) and water to a final volume of 50 μl ...
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bioRxiv - Molecular Biology 2020Quote: ... The plug was equilibrated twice in 1 ml of 1× M buffer (TaKaRa) by rotating the tube for 30 min at room temperature ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The PCR reaction included 2.5 µL of 10X buffer (Mg2+ plus) (TaKaRa, Japan), 1.5 µL of 25 mM MgCl2 ...
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bioRxiv - Immunology 2020Quote: ... and TDL (250 cells per subset) were sorted directly into lysis buffer (Takara) using a FACSAriaII (BD Biosciences ...
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bioRxiv - Cancer Biology 2021Quote: Tissues and cells were homogenized in 1 ml RNAisoTM Plus lysis buffer (TAKARA). Total RNA was extracted and 2 μg RNA was transcribed into cDNA with M-MLV reverse transcriptase (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... and purified cDNA was eluted in 17 µL elution buffer provided by Clontech. All samples were quantitated using Bioanalyzer 2100 Instruments (Agilent Genomics) ...
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bioRxiv - Neuroscience 2023Quote: ... The PCR reaction mixture for all reactions consisted of 5x PrimeSTAR Buffer (Takara), 200uM dNTPs (Takara) ...
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bioRxiv - Molecular Biology 2022Quote: ... supplemented with 0.1 U/μL of recombinant RNase inhibitor (wash buffer-RIn, Takara). Mechanical cell lysis was achieved by mixing each sample with 0.1 mm glass beads and grinding in a Retsch mixer mill at a frequency of 30/s for 5 min ...
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bioRxiv - Molecular Biology 2024Quote: ... The plug was equilibrated twice in 1 mL of 1× M buffer (TaKaRa) by rotating the tube for 30 min at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... 1µl of 10x Reaction buffer (SMARTer Ultra Low Input RNA Kit, Takara Bio) was added and the tube was vortexed immediately and stored at –80°C before being processed for library preparation ...
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bioRxiv - Molecular Biology 2024Quote: ... The plug was equilibrated twice in 1 mL of 1× M buffer (TaKaRa) by rotating the tube for 30 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... and purified cDNA was eluted in 17 μl elution buffer provided by Takara. All samples were quantitated using PicoGreen® on Molecular Dynamics M2 SpectraMax instrument ...
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bioRxiv - Neuroscience 2022Quote: ... that were filled with 2μl of picking buffer [1μL RNAse inhibitor (Takara #2313A) in 49 μL PBS] ...
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bioRxiv - Genomics 2024Quote: ... 1x Ex Taq buffer and 2 U Ex Taq polymerase (TaKaRa, Otsu, Japan). The thermal profile started with initial stage 94 °C for 3 min which was followed by 30 cycles of 1 min denaturation at 94 °C ...
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bioRxiv - Microbiology 2024Quote: ... Purified cDNA was then amplified by PCR in seqAMP DNA polymerase buffer (Clontech) with PCR primer F and R with unique sequencing barcodes (Table S5) ...
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bioRxiv - Cancer Biology 2024Quote: ... Lysis buffer was prepared containing per reaction: 125U RNase inhibitor (Takara Bio, 2313A), 2.5mM dNTP (NEB ...
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bioRxiv - Developmental Biology 2021Quote: ... DNA from these samples was screened by genotyping with Z-Taq (Takara R006B). Embryos identified as homozygous for wild type or HoxDinν2 alleles were processed individually for ATAC-Seq ...
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bioRxiv - Plant Biology 2021Quote: ... Samples were then prepared for sequencing using the SMARTer cDNA synthesis kit (Clontech) according to manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Total mRNA was isolated from tissue samples using TRIzol reagent (TAKARA, Tokyo, Japan) and was reverse-transcribed into cDNA using a high-capacity cDNA reverse transcription kit (TAKARA ...