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Citations for Takara Bio :
2801 - 2850 of 6487 citations for Cow G Protein Coupled Bile Acid Receptor 1 GPBAR1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... The libraries for Illumina sequencing were constructed using the ThruPLEX DNA-Seq Kit (Clontech) and purified using SPRIselect beads (Beckman Coulter) ...
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The spindle protein CKAP2 regulates microtubule dynamics and ensures faithful chromosome segregationbioRxiv - Cell Biology 2023Quote: ... the Tet-On® 3G Inducible Expression System kit was used (Takara Bio; 631167). RPE-1 wild-type and CKAP2-KO cells were transfected with a pEF1α-TET3G plasmid for expression of a Tet-On 3G transactivator protein ...
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bioRxiv - Neuroscience 2023Quote: ... cDNA was prepared from total RNA using the PrimeScript RT-PCR kit (Takara, #RR037Q). Relative gene expression of the cDNA was assayed using a 7500 Fast real-time PCR instrument (Applied Biosystems ...
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bioRxiv - Neuroscience 2023Quote: ... or control mCherry were subcloned using the InFusion HD cloning kit (Takara Bio USA). Furthermore ...
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bioRxiv - Microbiology 2023Quote: ... were ligated using In-Fusion HD Cloning Kits according to the manufacturer’s protocol (Takara). The resulting chimeras ...
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bioRxiv - Developmental Biology 2023Quote: The total RNA-seq libraries were constructed using the SMART-Seq Stranded Kit (Takara) as previously described (Wang et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... Plasmid extraction was performed using the NucleoBond Xtra Midi EF kit (Takara Bio, 740420.10).
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bioRxiv - Microbiology 2023Quote: ... cDNA was prepared using the PrimeScript II 1st Strand cDNA Synthesis Kit (TaKaRa Bio). The first targeted PCR was performed using a KAPA HiFi HotStart ReadyMix PCR Kit (Roche ...
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bioRxiv - Plant Biology 2023Quote: ... 32P-labeled dsRNAs were synthesized using the in vitro T7 Transcription kit (Takara Bio) with [α-32P]-UTP (PerkinElmer ...
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bioRxiv - Plant Biology 2023Quote: ... cDNA was synthesized from the PrimeScript™ RT reagent Kit with gDNA Eraser (TaKaRa) for RT-qPCR.
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bioRxiv - Cancer Biology 2023Quote: ... Virus was titered using the Lenti-X p24 Rapid Titer Kit (Takara Cat #632200).
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bioRxiv - Genetics 2024Quote: ... Extracted RNA was then reversely transcribed using the PrimeScript™ RT reagent Kit (Takara). RNA-seq libraries for expression analysis were constructed using KAPA RNA Hy-perPrep Kit KR1350 v1.16 according to the vendor’s protocol and paired-end 2 x 150 bp reads were sequenced using the Illumina HiSeq platform ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA was generated from total RNA using the Prime Script reagent kit (Takara #RR047A). Candidates of ΔPtth were characterized by the loss of DNA band in the deleted areas through PCR on the genomic DNA and cDNA ...
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bioRxiv - Cell Biology 2024Quote: ... Hippocampal neurons were transfected on DIV11-13 using the CalPhos Mammalian Transfection kit (Takara) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: cDNA was amplified using SMART seq v4 ultra-low input RNA kit (Takara Biosciences). 0.5 ng of amplified cDNA from EV-H and EV-C RNA was used as template ...
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bioRxiv - Cell Biology 2024Quote: ... using SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Takara, Kusatsu, Japan). The fragmentation time was adjusted depending on the quality of the RNA input ...
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bioRxiv - Microbiology 2024Quote: ... The cDNA synthesis was performed using PrimeScript 1st strand cDNA Synthesis Kit (TakaRa, 28704) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... the first PCR was performed using a Multiplex PCR Assay Kit Ver.2 (Takara) with MIG-seq primer set-1 developed by Suyama and Matsuki (2015) ...
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bioRxiv - Microbiology 2024Quote: ... Full-length cDNA was generated using the SMART-Seq® HT Kit (Takara Bio), according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... Total RNA was reverse transcribed to cDNA with M-MLV reverse transcriptase kit (Takara). LightCycler 480 SYBR Green I Master (Roche ...
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bioRxiv - Plant Biology 2024Quote: ... Extracted RNA was reverse transcribed to cDNA using a PrimeScriptTM RT reagent Kit (TaKaRa). The qRT-PCR was performed with SYBR Premix Ex TaqTM (TaKaRa ...
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bioRxiv - Immunology 2024Quote: ... RNA was converted to cDNA using PrimeScriptRT reagent Kit with gDNA Eraser (Takara, #RR047A), and used as a template for qPCR with TB Green® Premix Ex TaqTM II (Tli TNaseJ Plus ...
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bioRxiv - Molecular Biology 2024Quote: ... Multiplex TaqMan RT-qPCR was performed using One Step PrimeScript RT-PCR Kit (Takara) on StepOne Plus Real-time PCR machine (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2024Quote: ... total RNA was extracted from cells using the NucleoSpin RNA Plus Kit (Clontech, 740984.250). Following RNA extraction ...
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bioRxiv - Microbiology 2024Quote: Quantitative PCR was performed with the PrimeScript™ RT reagent Kit (#RR047A, Takara Bio). Briefly ...
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bioRxiv - Molecular Biology 2024Quote: ... The vg/cell was determined by real-time PCR using AAVpro Titration Kit (Takara) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... Primers with overhangs (Table S2) compatible with In-Fusion HD cloning kit (Takara Bioscience) were used to clone full-length genes into pESC-HIS (GenBank ...
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bioRxiv - Biochemistry 2024Quote: ... cDNA was synthesized using PrimeScript™ II 1st Strand cDNA Synthesis Kit (Takara Bio) according to the manufacturer’s recommendations ...
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bioRxiv - Plant Biology 2024Quote: ... cDNA was synthesized with the PrimeScript 1st strand cDNA Synthesis Kit (Takara, Cat# 6110A) with 1000 ng of total RNA ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and qRT-PCR was performed via a TB Green Premix ExTaq II Kit (Takara) on a Bio-Rad CFX-96 system (Bio-Rad ...
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bioRxiv - Genetics 2024Quote: ... cDNA was synthesized from total RNA using a PrimeScript RT reagent Kit (TaKaRa, Japan) and was used as a template to perform qPCR in SYRB green-based qPCR instrument (CFX-96 ...
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bioRxiv - Systems Biology 2024Quote: ... Mir-X miRNA qRT-PCR TB Green kit (Takara BIO, San Jose, CA, USA) was used for cDNA synthesis and miRNA expression analysis ...
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bioRxiv - Microbiology 2024Quote: ... These fragments were joined using an In-Fusion HD Cloning Kit (TaKaRa Bio, Japan). The primers used in this study are shown in Table 1.
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bioRxiv - Microbiology 2024Quote: ... was reverse-transcripted using PrimeScript II 1st Strand cDNA Synthesis Kit (TAKARA, Dalian, China) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: ... The extracted RNA was reverse-transcribed using the PrimeScript RT Reagent Kit (Takara Bio).
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bioRxiv - Physiology 2024Quote: ... cDNA was synthesized as described above using the PrimeScript RT Reagent Kit (Takara Bio).
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bioRxiv - Bioengineering 2024Quote: ... RNA was reverse transcribed using a PrimeScript™ RT Reagent Kit (Takara Bio Europe), and PCR reactions were performed using iTaq Universal SYBR Green Supermix (Bio-Rad) ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was synthesized using the PrimeScript 1st strand cDNA Synthesis Kit (Takara Bio USA). For RNA analysis of spirochetes in ticks ...
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bioRxiv - Molecular Biology 2024Quote: ... POINT5-seq libraries were made with the SMARTer Stranded RNA-Seq kit (Takara Bio) protocol ...
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bioRxiv - Plant Biology 2024Quote: ... cDNAs were synthesized using PrimeScript RT Kit with gDNA Eraser (Perfect Real Time; Takara) following the protocol of the manufacturer ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated with a mix of up to three primary antibodies simultaneously diluted in PBST with 1 % NDS overnight at room temperature with the following primary antibodies: rabbit anti-dsRed (1:1000; Clontech, AB_10013483), chicken anti-GFP (1:1000 ...
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bioRxiv - Neuroscience 2019Quote: ... and then incubated with a GFP antiserum (rabbit, 1:1000, Life Technology, #A6455) or an mCherry antiserum (mouse, 1:1000, Clontech, #632543). Primary antisera were diluted in PBS with 2% NGS overnight at 4°C ...
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Mis6/CENP-I maintains CENP-A nucleosomes against centromeric non-coding transcription during mitosisbioRxiv - Cell Biology 2021Quote: ... the rabbit anti-RNA polymerase II (phosphoS5) polyclonal antibody (1:100; ab5131) or rabbit anti-GFP polyclonal antibody (1:250; Clontech, 632592) was incubated with 200 µL of the lysate for 1 h at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... qRT-PCR was performed on 1 μL of 1 in 40 diluted cDNA in 15 μL reactions using SYBR Premix Ex-Taq II reagent (Takara Bio) in a Bio-Rad CFX96 real-time system (see Table S1 for primer pairs) ...
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bioRxiv - Genetics 2020Quote: ... Membranes were blocked in 5% milk for 1 h at room temperature and then probed with mouse-anti-EGFP (for pYFP constructs; 1:8000; Clontech, 632380) or mouse-anti-V5 tag (1:2000 ...
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bioRxiv - Neuroscience 2023Quote: ... They were then incubated overnight at 4°C with the following primary antibodies diluted in PBS - 0.3% Triton X100 - 1% NGS: rabbit anti-ZsGreen (1/1000, Clontech, 632474, RRID:AB_2491179) or rabbit anti- DCX (1/2000 ...
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bioRxiv - Developmental Biology 2023Quote: ... The following antibodies were used: rabbit or rat anti-dsRed (1:200, Clontech 632496 or 5F8 1:400, Chromotek 5f8-100); chicken anti-GFP (1:800 ...
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bioRxiv - Developmental Biology 2019Quote: To quantify EEC morphology score, chick anti-GFP (Aves GFP1010, 1:500 dilution) and rabbit anti-mcherry (TAKARA 632496, 1:250 dilution) antibodies were used in the fixed Tg(gata5:lifActin-EGFP);Tg(neurod1:TagRFP ...
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bioRxiv - Cell Biology 2022Quote: ... followed by SDS–PAGE and immunoblotting with goat anti-GST (1:3000, Cytiva, 27-4577-01) and mouse anti-6xHis (1:3000, Takara Bio, 631212) antibodies ...
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bioRxiv - Physiology 2022Quote: ... Cleared lysates were loaded into wells of the SDS-agarose gels (1% SeaKem Gold Agarose [Lonza, Basel, Switzerland], 30% glycerol, and 1 × Tris/glycine/SDS [TG-SDS] buffer [Takara Bio Inc.]) and then electrophoresed in a running buffer (1 × TG-SDS buffer and 10 mM 2-mercaptoethanol ...