Labshake search
Citations for Takara Bio :
2651 - 2700 of 5220 citations for Human Bcl2 Associated Death Promoter BAD ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... 0.5µg RNA was reverse transcribed into cDNA using the PrimeScript RT Reagent Kit (Takara, Kusatsu, Japan). Real-time qPCR was conducted on a Stepone plus real-time PCR system from Applied Biosystems using specific primers (Table 1 ...
-
bioRxiv - Molecular Biology 2021Quote: ... and assembled with PCR-amplified homology arms using In-Fusion® HD Cloning Kit (Takara 638920). Final products were verified by enzymatic digestion and sequencing ...
-
Induction of Dopaminergic Neurons for Neuronal Subtype-Specific Modeling of Psychiatric Disease RiskbioRxiv - Neuroscience 2021Quote: ... Ribosomal rRNA-depleted RNAseq libraries were prepared using the SMARTer Stranded RNA-Seq kit (Clontech, #634836) according to the manufacturer’s instructions with the following specifications ...
-
The pan-cancer lncRNA PLANE regulates an alternative splicing program to promote cancer pathogenesisbioRxiv - Cancer Biology 2020Quote: ... the products were purified using a MiniBEST Agarose Gel DNA Extraction Kit (Takara, #9762; Dalian, China), and subjected to in vitro transcription using a TranscriptAid T7 High Yield Transcription Kit (ThermoFisher Scientific ...
-
bioRxiv - Cancer Biology 2020Quote: ... Reverse transcription was carried out using the PrimeScript® RT reagent Kit with gDNA Eraser (TaKaRa), and cDNA products were amplified using SYBR ® Premix ExTaqTM II Kit (TaKaRa) ...
-
bioRxiv - Cell Biology 2021Quote: ... 3 h and 0 h before extracting total RNA by MiniBEST Universal RNA Extraction Kit (Takara). The abundances of the interest genes were detected measured in each time point by real-time quantitative PCR (qPCR ...
-
bioRxiv - Immunology 2021Quote: ... cDNA was synthesized from 1 μg of total RNA using an RNA reverse transcriptase kit (Takara). Real-time qPCR was performed using the ABI PRISM 7000 Sequence Detection System (Applied Biosystems) ...
-
bioRxiv - Cell Biology 2020Quote: ... The probe targeting the homology arm was labelled using the Ladderman Labelling kit (TaKaRa, Shiga, Japan) by PCR using the donor vector ...
-
bioRxiv - Cell Biology 2021Quote: ... ds-cDNA was made using Clontech SMARTer® Ultra Low RNA Kit (Clontech, Mountain View, CA) following manufacturer’s recommendations ...
-
bioRxiv - Biochemistry 2022Quote: ... The mutant derivatives of this vector were generated with the PrimeSTAR Mutagenesis Basal Kit (TAKARA-bio).
-
bioRxiv - Biochemistry 2022Quote: ... The molar concentration of the sequencing library was determined using the library quantitation kit from Takara Bio Inc ...
-
bioRxiv - Molecular Biology 2022Quote: ... and reversely transcribed into cDNA using the PrimeScript RT reagent Kit with gDNA Eraser (TAKARA, Japan). Primer sequences for target genes (HIF-1α ...
-
bioRxiv - Immunology 2022Quote: ... These RNA samples were reverse transcribed into cDNA using a PrimeScript RT reagent Kit (TaKaRa, Japan), and quantitative real-time PCR (q-PCR ...
-
bioRxiv - Immunology 2022Quote: ... cDNA was synthesized using the SMARTer RACE cDNA Amplification kit (Clontech, Palo Alto, California, United States). Amplification of the TCRβ VDJ region was performed using previously described primers and amplification protocols(45) ...
-
bioRxiv - Microbiology 2022Quote: ... Linearized vector and inserts were ligated using In-Fusion® HD Cloning Kit (Clontech, Takara, Japan) and transformed into StellarTM competent cells ...
-
bioRxiv - Microbiology 2022Quote: ... Samples were analyzed using a One-Step SYBR PrimerScript reverse transcription (RT)-PCR kit (TaKaRa, Japan) on Applied Biosystems QuantStudio ...
-
bioRxiv - Microbiology 2022Quote: ... and subjected to cDNA synthesis using a PrimeScript RT reagent kit (Perfect Real Time) (TaKaRa Bio) and random hexamer primers ...
-
bioRxiv - Genetics 2022Quote: ... and then used to synthesize cDNA with TaKaRa RNA PCR™ Kit (AMV) Ver.3.0 (TaKaRa). Quantitative real-time PCR (qPCR ...
-
bioRxiv - Neuroscience 2022Quote: ... and MEFs were reverse-transcribed using a PrimeScript II first-strand cDNA Synthesis Kit (Takara Bio) with a random hexamer primer ...
-
bioRxiv - Biochemistry 2022Quote: ... cDNA was synthesized with a Prime Script RT Reagent Kit (RR047A, Takara Bio Inc., Shiga, Japan). qRT-PCR was performed with SYBR Premix Ex TaqII (RR820A ...
-
bioRxiv - Cell Biology 2022Quote: ... Libraries were created by UCL Genomics Core Facility using the SMARTer Stranded Total RNAseq Kit (Clontech), cleaned using JetSeq (Bioline ...
-
bioRxiv - Cancer Biology 2022Quote: ... cDNA was generated from 1ng of input RNA using the SMART-Seq HT Kit (Takara 634455) at half reaction volume ...
-
bioRxiv - Cancer Biology 2022Quote: ... First-strand cDNA synthesis was performed with the Smart-seq v4 Ultra Low Input kit (Takara) using the 3’ Smart-seq CDS Primer IIA and Smart-seq V4 oligonucleotide ...
-
bioRxiv - Cancer Biology 2022Quote: Genotyping was done using the Terra PCR Direct Polymerase Mix and kits (Takara Bio; Shiga, Japan). The primers used for genotyping are given in supplementary table 1 ...
-
bioRxiv - Cell Biology 2022Quote: ... Total RNA (1 μg) was reversed to cDNA with PrimeScript™ RT reagent Kit (Takara, RR047Q), which was performed according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2022Quote: ... DNA for experiments was obtained using endotoxin-free plasmid purification kits (NucleoBond Xtra Midi EF, Takara, cat ...
-
bioRxiv - Neuroscience 2021Quote: ... ds-cDNA was prepared using the SMARTer Ultra Low RNA kit for Illumina Sequencing (Takara-Clontech) per manufacturer’s protocol using the lysis buffer as substrate for the reaction ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Samples were analyzed using a One-Step SYBR PrimerScript reverse transcription (RT)-PCR kit (TaKaRa, Japan) on an Applied Biosystems QuantStudio ...
-
bioRxiv - Developmental Biology 2021Quote: ... PrimeScript RT-PCR and SYBR Premix ExTaq Kits were from Takara (Cat#2641A, Cat#RR420, Japan). BCA protein assay and ECL kits were from Beyotime Institute of Biotechnology (Shanghai ...
-
bioRxiv - Plant Biology 2021Quote: ... We used SMART-Seq v4 Ultra Low Input RNA Kits for sequencing (Takara Bio Group; Japan), which first generated high-quality cDNA from ultra-low amounts of total RNA and then high-quality Illumina sequencing-ready libraries ...
-
bioRxiv - Molecular Biology 2020Quote: ... an RNA library was generated using SMARTer® Stranded Total RNA Sample Prep Kits (Takara Bio) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... cells were harvested and rAAV6 particles were purified using an AAVpro Purification Maxi kit (Takara, 6666) according to manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2021Quote: ... the first strand of cDNA was synthesized by using PrimeScript RT reagent kit (TaKaRa Bio Inc.) and not-so random primers ...
-
bioRxiv - Neuroscience 2021Quote: Total 500 ng RNA was used for cDNA synthesis using the PrimeScript Reverse Transcription Kit (Takara). Quantitative PCR was performed on a RotorGeneQ (Qiagen ...
-
bioRxiv - Immunology 2020Quote: ... followed by first-strand cDNA synthesis via PrimeScriptTM II 1st Strand cDNA Synthesis Kit (Takara, Japan) using miR-137- primer(5’-GTCGTATCCAGTGCAGGGTCCGAGGTCACTGGATACGACACGTG TAT-3’ ...
-
bioRxiv - Neuroscience 2021Quote: ... DNA was prepared using the endotoxin-free NucleoBond Xtra Maxi EF kit (Clontech, Takara Bio, USA).
-
bioRxiv - Microbiology 2021Quote: ... cDNA was synthesized from total RNA using a PrimeScript Perfect real-time reagent kit (Takara Bio) according to the instructions provided by the manufacturer ...
-
bioRxiv - Developmental Biology 2022Quote: ... and the cDNA was synthesized using the PrimeScript™ RT Reagent Kit with gDNA Eraser (Takara) as recommended by the manufacturer with an oligo dT used instead of random primers ...
-
bioRxiv - Developmental Biology 2022Quote: ... cDNA synthesis was performed with SMART- Seq v4 Ultra Low Input RNA Kit (Clontech cat. 634888) according to the manufacturer’s instruction ...
-
bioRxiv - Cell Biology 2022Quote: ... qPCR was performed using the SYBR Premix Ex Taq kit (TaKaRa Bio USA, San Jose, CA) and the CFX Connect real-time PCR machine (Bio-Rad ...
-
bioRxiv - Genetics 2022Quote: ... and then reverse transcribed RNA to get cDNA using PrimeScript RT reagent kit (Takara, Osaka, Japan). Supplementary Table 1 lists the primers used to construct minigene vectors and to detect alternative splice sites.
-
bioRxiv - Cancer Biology 2022Quote: ... cDNA was generated from 1ng of input RNA using the SMART-Seq HT Kit (Takara 634455) at half reaction volume followed by Nextera XT (Illumina FC-131-1024 ...
-
bioRxiv - Plant Biology 2022Quote: ... at the SalI and EcoRV restriction sites using an In-Fusion HD Cloning Kit (Takara Bio), which we transferred into the destination vector pGWB50157 using Gateway LR Clonase II Enzyme mix (Thermo Fisher Scientific) ...
-
bioRxiv - Microbiology 2022Quote: ... Total RNA (2 μg) was used for reverse transcription with the PrimeScriptTM RT reagent Kit (TaKaRa). Quantitative expression assays were performed by using the 2x M5 HiPer SYBR Premix EsTag kit (Mei5bio ...
-
bioRxiv - Biochemistry 2022Quote: ALP activities were measured using a Great EscAPe™ SEAP kit (Takara Bio Inc., Shiga, Japan) in lysates of PIGT+ and PIGT− cells ...
-
bioRxiv - Molecular Biology 2022Quote: ... The protein concentration was found to be 0.9 µg/µl using BCA Protein Assay Kit (TaKaRa).
-
bioRxiv - Genomics 2020Quote: ... Libraries were constructed using the Smart cDNA Library Construction kit from Clontech (Palo Alto, CA, USA) and normalized using the Trimmer cDNA Normalization kit from Evrogen (Moscow ...
-
bioRxiv - Immunology 2020Quote: ... cDNA was prepared using SMART-Seq v4 Ultra Low Input RNA Kit (Takara Bio USA, Inc). RNA sequencing libraries were prepared using the Illumina TruSeq™ RNA Sample Preparation Kit (Illumina ...
-
bioRxiv - Microbiology 2020Quote: ... The qPCR was performed with the MIR-X miRNA qRT-PCR TB Green Kit from TAKARA. To calculate the relative fold of expression of mir-243-3p between generations and genotypes we used delta-delta Ct calculations according to the Reference Unit Mass method (65) ...
-
bioRxiv - Molecular Biology 2021Quote: ... cDNA was prepared with 1 µg RNA and synthesized using the Primescript RT kit (Takara Inc.). qPCR assays were performed on the StepOnePlusTM Real-time PCR System (Applied Biosystems ...