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Citations for Takara Bio :
201 - 250 of 6767 citations for ssc mir 758 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... cDNA from a microgram of RNA was obtained using a PrimeScript RT reagent kit (Perfect Real Time, Takara Bio Inc., Otsu, Shiga, Japan) following its instructions ...
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bioRxiv - Plant Biology 2023Quote: Genomic DNA was removed from 1 μg purified RNA using gDNA Eraser (PrimeScript RT Reagent Kit, Perfect Real Time) following manufacturer’s instructions (Takara Bio Inc., Kusatsu, Japan). Reverse transcription and cDNA was synthesized using PrimeScript Reverse Transcriptase (TaKaRa Bio) ...
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bioRxiv - Cancer Biology 2020Quote: ... SYBR PrimeScript primary RT-PCR kit (Takara Bio, Japan) was used to evaluate the expression levels of METTL3 ...
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bioRxiv - Developmental Biology 2020Quote: ... retro-transcribed using the PrimeScript RT-PCR Kit (Takara) and analysed via qPCR using the SYBR qPCR Premix Ex Taq (Takara ...
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bioRxiv - Plant Biology 2021Quote: ... The first strand of cDNA was synthesized using PrimeScript™RT Master Mix (Perfect Real Time) (TaKaRa). Quantitative real-time PCR (RT-qPCR ...
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bioRxiv - Genetics 2020Quote: ... First-round reverse transcription PCR (RT-PCR) was conducted by using PrimeScript One Step RT-PCR Kit Ver.2 (TaKaRa, Dalian, China). A 10 μL reaction mixture contained 5 μL of 2 × 1 Step Buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was isolated using the TRIzol method and the expression of miRNAs analyzed by qRT-PCR with the Mir-X miRNA qRT-PCR TB Green kit (Takara Bio Inc ...
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bioRxiv - Molecular Biology 2022Quote: ... one subjected to RT-PCR using PrimeScript™ One Step RT-PCR Kit Ver.2 (Dye Plus) (Takara, Japan), then the region between S-5’UTR and N protein-ORF genes was amplified to monitor the expression of eGFP.
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bioRxiv - Microbiology 2020Quote: ... The qPCR was performed with the MIR-X miRNA qRT-PCR TB Green Kit from TAKARA. To calculate the relative fold of expression of mir-243-3p between generations and genotypes we used delta-delta Ct calculations according to the Reference Unit Mass method (65) ...
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bioRxiv - Developmental Biology 2021Quote: ... Real-time quantitative PCR was performed in triplicate using the SYBR Premix Ex Taq (Tli RNase H Plus) kit (Takara). The expression of TBP was used for the normalization of mRNA expression ...
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bioRxiv - Neuroscience 2020Quote: ... The virus titer of purified AAV stocks was determined by AAVpro® Titration kit (for Real Time PCR) ver2 (TaKaRa).
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bioRxiv - Molecular Biology 2023Quote: ... generated cDNA was subjected to real-time PCR analysis using SYBR® Premix Ex Taq™ II kit (TAKARA BIO) with the sets of specific primers (Table 1) ...
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bioRxiv - Neuroscience 2023Quote: ... Titration of the concentrated viruses was performed by AAVpro Titration Kit (for Real Time PCR) Ver.2 (Takara Bio Inc) following the manufacture’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... RT-PCR was carried out using the One Step RNA PCR Kit (TaKaRa Biotechnology Dalian ...
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bioRxiv - Biochemistry 2019Quote: ... Quantitative real-time PCR was performed with SYBR Premix Ex Taq (TaKaRa, Shiga, Japan) using Thermal Cycler Dice® Real Time System Single (TaKaRa ...
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bioRxiv - Plant Biology 2021Quote: ... Real-time PCR was performed using SYBR Premix Ex Taq II (TliRNaseH Plus) (TaKaRa). The level of GmSALT3 transcript was normalised using the control gene GmUKN1 (Hu et al. ...
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bioRxiv - Immunology 2020Quote: The real-time quantitative PCR was conducted with the Premix Ex Taq (Takara, Japan) to quantify the mRNA level ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Quantitative real-time PCR (qPCR) was done using Ex Taq SYBR Green (Takara-RR820A), gene specific primers (Table 1 ...
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bioRxiv - Biochemistry 2021Quote: ... real-time polymerase chain reaction (PCR) amplification was performed with SYBR Premix ExTag (Takara) on a QuantStudio™ 3 System (Applied Biosystems) ...
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bioRxiv - Immunology 2021Quote: ... Real-time PCR were performed with the SYBR Premix Ex Taq II (TAKARA#RR820A) in a LightCycler480 real-time PCR system ...
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bioRxiv - Cancer Biology 2021Quote: ... Quantitative real-time PCR (qPCR) was performed with TB Green Premix Ex Taq (Takara) using LightCycler 96 System (Roche Life Science ...
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bioRxiv - Neuroscience 2022Quote: ... Real-time PCR was performed using TB Green Premix Ex Taq II (Takara, Japan) and ViiA7 Real-Time PCR system (Applied Biosystems ...
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bioRxiv - Genetics 2023Quote: ... and used as templates for real-time PCR analysis using SYBR PreMix ExTaqII (Takara). The primers used are listed in Supplementary Table S3 ...
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bioRxiv - Neuroscience 2022Quote: ... Total RNA was reverse transcribed in the presence of PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) (Takara, Cat. No. RR047B). For each RT-qPCR reaction ...
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bioRxiv - Neuroscience 2022Quote: ... Total mRNA was reverse transcribed in the presence of PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) (Takara, Cat. No. RR047B). Droplet digital PCR was used to measure the levels of transcription factors in isolated MG following a previous method (Fisher et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... The expression of mRNA was examined by real-time quantitative-PCR (qRT-PCR) using SYBR Green PCR Master Mix (Takara, Japan) and a LightCycler480 Detection System (Roche ...
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Plastid double-strand RNA transgenes trigger small RNA-based gene silencing of nuclear-encoded genesbioRxiv - Plant Biology 2022Quote: ... Reverse transcription (RT) was performed using Advantage RT-for-PCR kit (Takara, San Jose, CA) following the manufacturer’s instructions with 20 μM oligo (dT)18 ...
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bioRxiv - Immunology 2023Quote: ... covering SIV env and nef were amplified from plasma viral RNA by nested RT-PCR using Prime-Script one-step RT-PCR kit v2 (TaKaRa) and KOD-Plus v2 (Toyobo) ...
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bioRxiv - Developmental Biology 2023Quote: ... and RT-PCR was performed to detect sorcs2 mRNA in wt embryos using the Titanium One-Step RT-PCR kit (Clontech) with primers 5’-TTTTGCGCACCTGTACCCAGCTG-3’ and 5’-TAACGCGCTCCTGAAGCAGAGTC-3’ for detection of mRNA after injection of different amounts of sMO and 5’-TTTTGCGCACCTGTACCCAGCTGTGTT-3’ and 5’ TAACGCGCTCCTGAAGCAGAGTCCATT-3’ for the different developmental stages ...
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bioRxiv - Pathology 2022Quote: ... Single-stranded cDNA was reverse transcribed using Prime Script RT Master Mix (Perfect Real Time) (Takara Bio Inc.), and used for subsequent real-time PCR reactions ...
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bioRxiv - Molecular Biology 2023Quote: ... and first-strand cDNA was synthesized using PrimeScript RT Master Mix (Perfect Real Time) (RR036Q; TaKaRa, Ohtsu, Japan) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... and cDNAs was synthesized from total RNA using the PrimeScript™ RT Master Mix (Perfect Real Time, Takara). Quantitative real-time PCR was performed in triplicate using PowerUp SYBR Green (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... RT-qPCR was performed using SYBR® Premix Ex Taq™ II (Perfect Real Time, Takara Bio Inc.). SYBR green detection of PCR products was conducted in real time using a MyiQ single-color detection system (Bio-Rad ...
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bioRxiv - Genomics 2021Quote: ... PrimeScript High Fidelity RT-PCR Kit (Takara Bio, Japan, R022A), In-Fusion Snap Assembly Master Mix (Takara Bio ...
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bioRxiv - Developmental Biology 2020Quote: ... PrimeScript II High Fidelity One step RT-PCR Kit (Takara) was used with the following primers ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... SYBR RT-PCR Kit was purchased from Takara (Dalian, China). PHrodo-labelled E ...
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bioRxiv - Cancer Biology 2021Quote: ... and quantitative real-time PCR was performed using the SYBR® Premix Ex Taq II Reagent Kit (Takara, Cat. Cat. #RR820A) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: ... The LightCycler 480II PCR system was programmed according to the instructions of the SYBR Premix Ex Taq II (Perfect Real Time) kit (TaKaRa, China) to conduct fluorescence-based quantitative PCR ...
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bioRxiv - Microbiology 2021Quote: ... leuC and leuD were measured by qRT-PCR using a SYBR Premix Ex Taq (perfect real time) kit (TaKaRa, Dalian, China). The recA gene was used as an internal control for B ...
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bioRxiv - Microbiology 2022Quote: ... qTOWER3 G Real-Time System (Analytik Jena) Thermal Cycler Dice Real Time System III (Takara) or 7500 Real-Time PCR System (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2023Quote: ... qTOWER3 G Real-Time System (Analytik Jena) Thermal Cycler Dice Real Time System III (Takara) or 7500 Real-Time PCR System (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2020Quote: ... Quantitative real-time PCR (qRT-PCR) was performed in a 20 μL reaction containing SYBR Green (TaKaRa, Kusatsu, Japan) on MXP3000 cycler (Stratagene ...
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bioRxiv - Microbiology 2020Quote: ... The number of viral copies was detected by real-time quantitative reverse transcription PCR (qRT-PCR) (Takara Cat no.RR820A) to reflect the replication efficiency of the virus ...
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bioRxiv - Microbiology 2020Quote: ... was reverse-transcribed to cDNA using random hexamers and oligo (dT) by following the instructions provided in the Prime-Script RT Reagent Kit (Perfect Real Time from TaKaRa Bio Inc., Otsu Shiga, Japan). RT-qPCR was carried out in a StepOnePlus Real-Time PCR system (Applied Biosystems ...
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bioRxiv - Biochemistry 2022Quote: ... one microgram of total RNA was employed to obtain the corresponding cDNA target sequences using an oligo(dT)18 primer and the PrimeScript RT reagent kit (Perfect Real Time, Takara Bio Inc., Otsu, Shiga, Japan), following the manufacturer’s directions ...
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bioRxiv - Microbiology 2019Quote: ... 300 ng of each RNA was reverse transcribed to cDNA using random hexamers and oligo(dT) primers and following the instructions provided in the PrimeScript RT Reagent Kit (Perfect Real Time from Takara Bio Inc., Otsu Shiga, Japan). RT-qPCR was carried out in a StepOnePlus Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Microbiology 2022Quote: ... DNAse treated-RNA was reverse transcribed into cDNA using random hexamers and oligo (dT) primers with the Prime-Script RT Reagent Kit (Perfect Real Time from Takara Bio Inc., Otsu Shiga, Japan). Viral presence was assessed through RT-qPCR (StepOnePlus Real-Time ...
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bioRxiv - Immunology 2022Quote: ... added with RT-PCR master mix and IgG VH/VL primers per well and performed with RT-PCR following the one step RT-PCR kit protocol (Takara, RR057A). Then ...
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bioRxiv - Genetics 2021Quote: Isolated RNA from OSF and control tissues was immediately subjected to realtime RT PCR (using Takara PrimescriptTM one step realtime RT PCR kit) (Takara, Japan) analysis for detection of changes in expression of NQO1 mRNA according to the manufacturers protocol ...
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bioRxiv - Microbiology 2022Quote: ... the viral RNA was isolated and amplified by RT-PCR using the PrimeScript II High Fidelity One Step RT-PCR Kit (Takara Bio) and specific primers ...