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Citations for Takara Bio :
201 - 250 of 1209 citations for Human PIGV shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Normal human stomach tissue RNAs were purchased from TaKaRa Bio (Shiga ...
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bioRxiv - Immunology 2019Quote: ... and Retronectin (Recombinant Human Fibronectin) was purchased from Takara Bio (Saint-Germain-en-Laye ...
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bioRxiv - Neuroscience 2020Quote: Human induced pluripotent stem cells (iPSCs) (ChiPSC18, Takara Bioscience) were reprogrammed using a protocol for midbrain dopaminergic neurons adapted from Kirkeby et ...
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bioRxiv - Cell Biology 2020Quote: Human telomerase-immortalized retinal-pigmented epithelial cells (RPE1; Clontech) either expressing LifeAct-GFP or parental (Vignaud et al. ...
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bioRxiv - Biochemistry 2022Quote: ... human CCDC22 was first cloned into pEGFP-C1 (Clontech) between Sal1 and BamH1 ...
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bioRxiv - Microbiology 2023Quote: ... and the Mate and Plate Universal Human Library (Clontech). This yeast two-hybrid universal library was constructed from human cDNA that has been normalized to remove high copy number cDNAs (overrepresented transcripts ...
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bioRxiv - Genetics 2023Quote: ... Human KISS1 was cloned into pLVX-neo vector (Clontech) for overexpression ...
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bioRxiv - Cell Biology 2023Quote: ... anti-human specific GFAP (hGFAP, TAKARA, Stem123, 1:1000), anti-AQP4 (Cell Signaling ...
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bioRxiv - Cancer Biology 2023Quote: ... Human iPSC line ChiPSC22 (Cellartis/Takara Bio Europe AB) was cultivated in the feeder-free DEF-CS system (Cellartis/Takara Bio Europe ...
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bioRxiv - Neuroscience 2022Quote: ... Plasmid expressing MeCP2 and GFP tagging was made by cloning the cDNA into pLVX-EF1α-IRES-ZsGreen1 vector (TaKaRa plasmid #631982). R168X mutation was introduced by site-directed mutagenesis ...
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bioRxiv - Microbiology 2020Quote: ... Construction of strains with cytoplasmic sGFP expression was performed by cloning the sGFP expression cassette from plasmid pIGPAPA and the neo cassette from plasmid pSD1 to the polylinker of plasmid pBluescript II (using the T4 DNA ligase, Takara Bio). The resulting plasmid pBS-GFP-gen was used to transform V ...
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bioRxiv - Microbiology 2022Quote: ... and the plasmid DNAs were isolated using the NucleoBond® Xtra Midi EF plasmid mid-prep kit (Takara Bio USA Inc.) according to the manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2022Quote: Single point mutations in all mOptoT7 plasmids were based on previously published plasmids (mOptoT7 Version 1, V1)53 and created using CloneAmp HiFi PCR Premix (Takara Bio). All constructs were transformed into Top10 E ...
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bioRxiv - Cell Biology 2023Quote: ... and constructs were isolated from 50 ml bacterial cultures using a plasmid DNA extraction kit (Takara Bio USA, Inc. NucleoSpin Plasmid). DNA concentrations were measured by a NanoDrop spectrophotometer ...
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bioRxiv - Physiology 2024Quote: The pGG2-TNNT2-HA-TTL and the pAAV-TNNT2-Empty plasmids were generated from the pGG2-TNNT2-Mut1 plasmid (VC206) by InFusion Cloning (Takara Clontech). In brief ...
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bioRxiv - Molecular Biology 2020Quote: ... JEG3 cells (human; sex: female, placenta epithelial), HEK293 derivative Lenti-X™ 293T cells (human; sex: female, kidney epithelial) obtained from Takara (cat. 632180), Huh-7.5 heptoma cells (human ...
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Flexible linkers in CaMKII control the balance between activating and inhibitory autophosphorylationbioRxiv - Biophysics 2019Quote: Human CaMKII-α (Uniprot_ID: Q9UQM7) and human CaMKII-β (Uniprot_ID: Q13554) were cloned into the pEGFP-C1 vector backbone (Clontech, Mountain View, CA), after modifying the vector to contain a biotinylation sequence (Avitag ...
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bioRxiv - Neuroscience 2022Quote: ... We constructed the dlg1[4K] donor plasmid (Fig. 1) by joining five fragments into a minimal (AMP and ORI) plasmid backbone by In Fusion assembly (Takara, no. 639649). A list of primers used ...
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bioRxiv - Cell Biology 2023Quote: ... the gateway cassette of the plasmid pmCherry-ccdB was generated by subcloning of ccdB into pmCherry-C1 plasmid (Clontech, Mountain View, CA) using SmaI ...
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bioRxiv - Microbiology 2024Quote: ... The PCR products were cloned into a PCR linearized pEcgRNA-guide plasmid to make the pEcgRNA-guide-RT plasmid using In-fusion® HD-cloning kit (Takara Bio) following the manufacturer’s instructions.
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bioRxiv - Biophysics 2022Quote: ... A Puromycin resistance plasmid (pPUR, Clontech, Cat No. 631601) was used as a selection marker ...
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Inositol depletion regulates phospholipid metabolism and activates stress signaling in HEK293T cellsbioRxiv - Cell Biology 2022Quote: ... coli using the NucleoSpin Plasmid miniprep kit (Takara 740588). Transformants were sequenced to confirm successful cloning ...
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bioRxiv - Genetics 2019Quote: ... using the pEGFP-1 plasmid (Clontech, Mountain View, CA) as template ...
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bioRxiv - Microbiology 2019Quote: ... were co-transformed with pGro7 plasmid (Takara Bio Inc.) into E ...
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bioRxiv - Neuroscience 2020Quote: ... Plasmids for the expression of eGFP (Clontech, Kyoto, Japan) were co-transfected in order to identify cells expressing the plasmids ...
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bioRxiv - Biochemistry 2020Quote: ... and cloned into the expression plasmid pEGFP-N1 (Clontech) after the removal of the cDNA region encoding EGFP ...
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bioRxiv - Developmental Biology 2020Quote: ... p2lox plasmids were generated by In-Fusion cloning (Takara) the respective cDNAs into the p2lox plasmid backbone ...
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bioRxiv - Microbiology 2021Quote: ... These fragments were then cloned into pQCXIP plasmid (Clontech) between SbfI and NotI restriction sites ...
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bioRxiv - Cell Biology 2020Quote: The eGFP-C1 plasmid (kanamycin resistant; Clontech; 4731 bp) was used to generate N-terminal GFP-tagged Rab46 mutants ...
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bioRxiv - Biochemistry 2020Quote: ... Takara Clontech pTRE3g bidirectional plasmid (Takara Clontech Cat# 631337) for inducible expression was subcloned from Addgene plasmids #89470 & #89471 ...
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bioRxiv - Biochemistry 2020Quote: ... Takara Clontech pTRE3g bidirectional plasmid (Takara Clontech Cat# 631337) for inducible expression was subcloned from Addgene plasmids #89470 & #89471 ...
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bioRxiv - Cell Biology 2021Quote: ... Previously built plasmids derived from pmCherry-N1 (Takara-Clontech) and piRFP (a gift from Michael Davidson & Vladislav Verkhusha ...
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bioRxiv - Cell Biology 2021Quote: ... Previously built plasmids derived from pmCherry-N1 (Takara-Clontech) and piRFP (a gift from Michael Davidson & Vladislav Verkhusha ...
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bioRxiv - Cell Biology 2021Quote: ... a plasmid expressing enhanced green fluorescent protein (EGFP) (Clontech), or in another series of experiments with pVSV-G ...
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bioRxiv - Molecular Biology 2022Quote: ... Plasmids were transferred to pGADT7 and pGBKT7 vectors (Clontech) by LR reaction (Invitrogen).
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bioRxiv - Evolutionary Biology 2022Quote: ... with the chaperone plasmid pG-KJE8 (Takara Bio Inc.), was selected for AnMurE_pPROEX ...
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bioRxiv - Biophysics 2022Quote: ... A Puromycin resistance plasmid (pPUR, Clontech, Cat No. 631601) was used as a selection marker ...
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bioRxiv - Synthetic Biology 2022Quote: Plasmids were transformed into Stellar competent cells (Takara Bio), and transformed cells were grown at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... The following plasmid constructs were used: pmCherry-N1 (Clontech), pEGFP-N1 (Clontech) ...
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bioRxiv - Biochemistry 2020Quote: ... were co-transformed with pGro7 plasmid (Takara Bio Inc.) into E ...
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bioRxiv - Neuroscience 2019Quote: ... Plasmid amplification was performed using Stellar (Clontech Laboratories Inc.) or NEB10-beta (New England BioLabs Inc. ...
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bioRxiv - Developmental Biology 2020Quote: ... was cloned into pLVX Tet-One Puro plasmid (Clontech), packaged in 293T cells (from ATCC ...
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bioRxiv - Physiology 2021Quote: ... with 200 ng of GFP plasmid (TaKaRa, Shiga, Japan) and with 300 ng of cDNA plasmid encoding wild-type or mutant human TRPA1 (pCMV6-XL4 vector ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The remaining plasmids were treated with DNase I (Takara), and the transcribed RNAs were purified using the RNeasy Mini kit (Qiagen).
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bioRxiv - Microbiology 2019Quote: For the Tet-ON inducible expression plasmid pTRE3G (Clontech), WH1(WT)-TagGFP2 and TagGFP2 genes were amplified by PCR (5’-GAAGATCTATGGGCAGCCATCATCATCA and 5’-CCGGAATTCCATATGTTACCTGTACAGCCTC primers) ...
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bioRxiv - Immunology 2020Quote: ... Plasmids were transformed into Stellar Competent Cells (Takara Bio) and transformed cells were grown at 37 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... These plasmids were co-transfected with pEGFP-N1 (Clontech) into O9-1 using Lipofectamine 2000 (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2023Quote: ... TRE3G promoter from pLVX-TRE3G plasmid purchased from Clontech was PCR amplified with Kpn1 in forward primer and Aar1 in reverse primer ...
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bioRxiv - Neuroscience 2023Quote: ... Plasmids were created using In-Fusion Cloning (Takara Bio) or the Gateway recombination (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: Assembled plasmid was transformed into Stellar Cells (Takara #636766). 50 uL of competent cells were pre-incubated with 2.5 uL of the in-fusion reaction mix for 30 minutes on ice ...