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Citations for Takara Bio :
201 - 250 of 2084 citations for HLA A*0201 WT 1 complex Protein Human HEK293 His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... The nucleotide sequence encoding for human CyPD was obtained from a Human cDNA placenta library (Clontech) by PCR ...
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bioRxiv - Developmental Biology 2024Quote: ... Full-length cDNA fragment of human MAK was amplified by PCR using human retinal cDNA (Clontech) as a template and subcloned into the pCAGGSII-2xHA vector (62) ...
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bioRxiv - Microbiology 2020Quote: Human embryonic kidney 293T (Clontech, 632180), human lung carcinoma A549 (ATCC ...
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bioRxiv - Developmental Biology 2024Quote: ... Human cDNA was purchased from Clontech. The cDNAs were used for PCR with rTaq polymerase (TaKaRa) ...
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bioRxiv - Cell Biology 2022Quote: ... The library was generated by using the SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian (Clontech) with 1 μg RNA as input ...
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bioRxiv - Pathology 2020Quote: ... RBD-scFv was purified from the supernatant using Capturem™ His-Tagged Purification Miniprep Kit (Takara Bio). One prep of 800 μL supernatant through one column of the kit yielded 102 μg/mL of RBD-scFv measured by NanoDrop™ 2000/2000c Spectrophotometers (ThermoFisher) ...
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bioRxiv - Molecular Biology 2020Quote: ... Fractions were precipitated with trichloroacetic acid (TCA) and subjected to Western blot analysis (anti-His antibody, Clontech). Additionally ...
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bioRxiv - Bioengineering 2023Quote: ... Yeast transformants were selected by growth on synthetic dropout nutrient medium SD/-Leu/-His/-Ade/-Trp (Clontech) supplemented with 40mg/LX-α-gal (5-bromo-4-chloro-3-indolyl-a-D-galactopyranoside ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The selected yeast strains were seeded in SD medium with Dropout (DO) supplement-His (Clontech, CA, US) on a sterile black clear-bottom 384-well plate (Corning ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 2022) was also cloned into the pIZ/V5-His vector using the KpnI and NotI enzymes (TaKaRa).
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bioRxiv - Biophysics 2024Quote: ... Cells were harvested and purified on Talon His-Tag Purification Resin (Takara Bio, Mountain View, CA, USA). The hexahistidine tag was removed by incubating overnight at 4°C with TEV protease ...
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bioRxiv - Biochemistry 2024Quote: ... His-tagged ectodomain was purified from clarified supernatants using 2 mL of cobalt resin (Takara Bio TALON) plus 18 µl 1 M CoCl2 to compensate for cobalt leaching away with the Expi293 Expression Medium ...
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bioRxiv - Plant Biology 2021Quote: ... Transferred proteins were probed with anti-GFP (Takara Bio, 1:5000 dilution; Catalog # 632380) or anti-tubulin (Sigma ...
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bioRxiv - Bioengineering 2021Quote: ... Human umbilical vein endothelial cells (HUVECs) and human aortic smooth muscle cells (HAoSMCs) were purchased from TAKARA BIO (Tokyo ...
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bioRxiv - Genomics 2020Quote: The amplified library was then recombined with pCMV FAS wt minigene exon 5-6-7 (Förch et al., 2000) using the In-Fusion HD Cloning kit (639649, Clontech) in a 1:8 vector:insert optimized ratio and transformed into Stellar competent cells (636766 ...
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TIPRL1 and its ATM-dependent phosphorylation promote radiotherapy resistance in head and neck cancerbioRxiv - Cancer Biology 2023Quote: ... Wild-type (WT) TIPRL1 cDNA was cloned into 3xFLAG pLenti6.4 using In-Fusion® HD Cloning Kit (Takara, #639650). Phosphomimic and non-phospho mutants (S265D and S265A ...
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bioRxiv - Cell Biology 2023Quote: ... 13.32 (WT female) and 13.33 (WT male) were transduced with virus bearing either the empty pLVX-EF1a-IRES-ZsGreen1 vector (Takara), or the same plasmid expressing the coding sequence of C181 as described in lentivirus transduction ...
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bioRxiv - Cell Biology 2024Quote: ... 13.32 (WT female) and 13.33 (WT male) were transduced with virus bearing either the empty pLVX-EF1a-IRES-ZsGreen1 vector (Takara), or the same plasmid expressing the coding sequence of C181 as described in lentivirus transduction ...
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bioRxiv - Cell Biology 2020Quote: ... To detect the transgenic GFP-tagged Abl proteins we used anti-GFP (JL-8, 1:500 or 1:1000, Clontech). Anti-αTubulin (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... 1.25 x105 cells/cm2 were immediately added to each well with HEK293 maintenance media modified with 10% tetracycline-free FBS (Takara Bio, Cat. No. 631367). After 24 h ...
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bioRxiv - Cancer Biology 2024Quote: ... sections were stained overnight in 4°C with primary antibodies: human cytoplasmic antigen (1:1000, clone STEM121, Takara, Y40410), OLIG2 (1:1000 ...
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bioRxiv - Plant Biology 2022Quote: ... and His (-HTLA) but containing 5-bromo-4-chloro-3-indolyl α-D-galactopyranoside (Clontech, Madison, WI, USA). As a control ...
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bioRxiv - Molecular Biology 2023Quote: ... and RNA-sequencing libraries were made using the SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian (Takara). Libraries were sequenced at the Bauer Core Facility (Harvard University ...
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bioRxiv - Cell Biology 2023Quote: ... RNA-seq libraries were prepared with the SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian (Takara #634875). 75 bp single-end sequencing was performed using an Illumina NextSeq500 system at the CRI at UT Southwestern Sequencing Facility ...
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bioRxiv - Genomics 2023Quote: ... Libraries were prepared using the SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian (Takara Bio USA, Inc.), which incorporates both RiboGone and SMART (Switching Mechanism At 5’ end of RNA Template ...
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bioRxiv - Plant Biology 2023Quote: ... Ade and His but containing 5-Bromo-4-Chloro-3-indolyl a-D-galactopyranoside (X-a-gal) (Clontech). To detect interactions ...
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bioRxiv - Cell Biology 2023Quote: ... Sequencing libraries from treated RNA were created using the SMARTer Stranded Total RNA HI Mammalian kit (Takara 634873) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: A pull-down assay was performed using a Capturem His-Tagged Purification Miniprep Kit (#635710, Takara, Shiga, Japan). 50 µg of BSA was added to the column for blocking ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA of Human Phosphodiesterase type 10 (hPDE10) was amplified by conventional nested PCR using human brain cDNA (Clontech). cDNAs template encoding wild-type and D674A mutant form of hPDE10 catalytic domain (CD ...
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bioRxiv - Developmental Biology 2024Quote: ... Human full-coding KATNA1 cDNA was isolated from a human fetal brain Marathon-ready cDNA collection (Clontech, Invitrogen) with the following forward and reverse primers ...
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bioRxiv - Microbiology 2021Quote: ... protein expression was induced with 1 mM of isopropyl-β-D-thiogalactopyranoside (IPTG; Takara Bio) at the final concentration at 25°C ...
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bioRxiv - Neuroscience 2021Quote: Human Brain Total RNA (Takara Cat. #636530) and Human Brain Cerebral Cortex Total RNA (Takara Cat ...
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bioRxiv - Bioengineering 2021Quote: Human embryonic kidney (HEK293T) cells (632180; Takara) were cultured in DMEM (10-013-CV ...
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bioRxiv - Cell Biology 2022Quote: ... Human embryonic kidney epithelial Lenti-X293T (Clontech) cells were cultured in complete DMEM (Sigma Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... Human Universal QUICK-Clone cDNA II (Clontech) for a template cDNA ...
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bioRxiv - Cell Biology 2023Quote: Human cardiomyocyte cells were purchased from Takara Bio (ref Y10060) ...
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bioRxiv - Cell Biology 2020Quote: Flp-In™ T-REx™ 293 cell lines stably transfected with Flag-BirA*-TopBP1 WT or W1145R grown to 75% confluence were incubated with 1µg/ml of doxycycline (Clontech, 631311) for 16h and with 50µM biotin for 3 or 16 hours ...
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bioRxiv - Molecular Biology 2021Quote: ... UAS-dH1::GFP and UAS-dH1K27A::GFP stocks were prepared by inserting the corresponding ORFs (WT or carrying a K27A mutation generated by PCR) into pEGFP (Clontech) to generate fusion proteins and then the inserts were cloned into pUAST (details can be provided on request) ...
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bioRxiv - Microbiology 2021Quote: ... CPER fragments containing WT or mutated or 3’UTRs were amplified from the plasmids using PrimeStar GXL polymerase (Takara, Japan) and gel-purified ...
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bioRxiv - Plant Biology 2021Quote: ... The EVD coding sequence (At5TE20395) was directly amplified from wt Col DNA using primers containing appropriate plasmid homology for In-Fusion Cloning (Clontech) into their respective digested binary vector backbones ...
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bioRxiv - Cell Biology 2021Quote: ... WT TARPs and ncAA-tagged TARPs were subcloned into a bidirectional doxycycline-inducible expression vector pTRE3G-BI (Takara Bio, #631332) using the restriction sites KpnI/XbaI ...
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bioRxiv - Cell Biology 2021Quote: ... mCherry-STIM1-10A and WT STIM1 were constructed by inserting PCR fragments into XhoI-HpaI sites of pMSCV-puro vector (Clontech) and were packaged using the Phoenix-ECO cell line (ATCC CRL-3214) ...
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bioRxiv - Microbiology 2022Quote: RNA isolation was performed from transfected cells / after appropriate hours of infection with STM WT at MOI of 10 using TRIzol (Takara) reagent according to manufacturers’ protocol ...
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bioRxiv - Plant Biology 2023Quote: ... was PCR amplified from the pET-28a plasmid bearing WT or individual six-point mutations (M1 to M6) using HS Primestar DNA polymerase (Takara) with forward and reverse primers bearing BamHI and XhoI ...
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bioRxiv - Cell Biology 2023Quote: ... plus any amplicon smaller than the 9246 bp WT band for the large indel screen were gel purified with the NucleoSpin Gel clean up kit (Takara Bio ...
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bioRxiv - Developmental Biology 2023Quote: ... and RT-PCR was performed to detect sorcs2 mRNA in wt embryos using the Titanium One-Step RT-PCR kit (Clontech) with primers 5’-TTTTGCGCACCTGTACCCAGCTG-3’ and 5’-TAACGCGCTCCTGAAGCAGAGTC-3’ for detection of mRNA after injection of different amounts of sMO and 5’-TTTTGCGCACCTGTACCCAGCTGTGTT-3’ and 5’ TAACGCGCTCCTGAAGCAGAGTCCATT-3’ for the different developmental stages ...
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bioRxiv - Cell Biology 2024Quote: ... where RapA(wt) was amplified from AX2 cDNA and inserted into pGBKT7 (Matchmaker™ GAL4 Two-Hybrid System 3, Clontech) using BamHI/PstI sites ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentrations were measured using BCA Protein Assay Kit (TaKaRa). The samples were subjected to SDS-PAGE ...
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bioRxiv - Genomics 2021Quote: U2OS (human bone osteosarcoma epithelial, female, ATCC HTB-96) and LentiX 293T (human embroyonic kidney epithelial, female, Takara 632180) cells were cultured in Dulbecco’s modified Eagle medium (DMEM ...
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bioRxiv - Cell Biology 2023Quote: A cDNA fragment encoding human FAM53C was isolated by amplifying with nested PCR from human cDNA library plasmid (Takara). The oligonucleotide primer sequences used for the 1st PCR are 5′-CAAAGTGTGCAAGTCAAATCCTGG-3′ (5′ upstream ...