Labshake search
Citations for Takara Bio :
201 - 250 of 279 citations for Chocolate Agar with Bacitracin for Haemophilus isolation 15x100mm plate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: Transfection of Halo-tagged AnkB440 plasmids were conducted in HEK293T cells grown in 10 cm culture plates using the calcium phosphate transfection kit (Takara) and 8 µg of plasmid ...
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bioRxiv - Molecular Biology 2021Quote: ... First strand cDNA was synthesized with 3.6 μg of total RNA using Make your own ‘mate & plate’ library system (Takara, Clontech) according to the manufacturer’s instruction ...
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FTSH3 facilitates complex I degradation through a direct interaction with the complex I subunit PSSTbioRxiv - Plant Biology 2023Quote: ... Mating was carried out in a 96-well plate according to manufacturer’s instructions (Matchmaker GAL4 Two Hybrid System (Clontech/TakaraBio). Mated diploid strains were serially diluted and plated on - Leu–Trp double dropout (DDO ...
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bioRxiv - Plant Biology 2023Quote: ... The cDNA library was prepared from rice flowers and ligated to the pGADT7-Rec vector using the Make Your Own “Mate and Plate” Library System (Takara). A second cDNA library was prepared from rice leaves under salt stress ...
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bioRxiv - Immunology 2023Quote: ... and GC B cells (live singlet CD19+ CD4− IgDlo CD71+CD38int CD20+ CXCR5+) were sorted using a FACSAria II into 96-well plates containing 2 μL Lysis Buffer (Clontech) supplemented with 1 U μl−1 RNase inhibitor (NEB) ...
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bioRxiv - Cancer Biology 2024Quote: ... approximately 20% confluent HEK293T cells seeded in 12-wells plates were transfected with 300ng ZNRF3 encoding plasmid and 50ng GFP expression plasmid (pEGFP-C1, Clontech) using Lipofectamine 2000 ...
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bioRxiv - Cancer Biology 2024Quote: ... Meanwhile, untreated 6-well cell culture plates (Falcon, 351146) were coated with 15 µg/ml retronectin solution (Takara Bio, T100A) overnight at 4 °C ...
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bioRxiv - Genetics 2024Quote: ... 0.2*106 mESCs were seeded in a well of a 12-well plate in 2iL and transduced the next day with 1ml of 5x concentrated (lenti-X, Clontech) and filtered viral supernatant with 8 ng/μl polybrene (Sigma Aldrich) ...
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bioRxiv - Plant Biology 2023Quote: ... the Y2HGold strain was transformed with the bait vector (pGBKT7-SR45) and then mated with strain Y187 containing an Arabidopsis cDNA library (Mate and Plate Library-Universal Arabidopsis, Clontech) (Stankovic et al. ...
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bioRxiv - Immunology 2023Quote: ... into 96-well plates containing 12.5μl CDS sorting buffer as described in the SMART-Seq® HT Kit protocol (Takara Bio). Full-length cDNA amplification of single cells was performed per the kit protocol (Takara Bio) ...
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bioRxiv - Cell Biology 2023Quote: ... Fractionated CD34+ cells from Animals #2 and #3 were cultured overnight on RetroNectin-coated plates (Takara, T100B, Mountain View, CA) in X-VIVOTM 10 (Lonza ...
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bioRxiv - Neuroscience 2024Quote: ... and re-plated at a concentration of 2.5×105 cells/cm2 onto plates coated with 4 μg/mL iMatrix-511 (Takara Bio) in N2B27 medium supplemented with 10 μg/mL BDNF and 10 µg/mL Rock Inhibitor Y-27632 ...
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bioRxiv - Molecular Biology 2023Quote: Lentivirus was produced using a standard protocol64 from a 15 cm plate of 25M lenti-x 293T cells (Clontech #632180) via polyethylenimine (PEI ...
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bioRxiv - Microbiology 2022Quote: ... A tomato Mate & Plate library was constructed and used for screening with the Matchmaker Gold Yeast Two-Hybrid System (Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: iPSCs SFCi55 and hESCs RUNX1-GFP were plated at 3 × 105 cells per a well of a 6 well plate and reverse transfected with 2 μg of DNA using the Xfect Transfection reagent (Clontech) and analyzed 2 days later.
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bioRxiv - Immunology 2022Quote: ... Lentiviral supernatants were spun onto nontreated wells plates coated with 30 μg/mL Retronectin reused up to three times (T100B, Takara), at 2000 x g for 2 hours at 32°C and replaced with activated human CD8+ T cells in fresh RPMI supplemented with 30 U/mL IL-2.
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bioRxiv - Plant Biology 2022Quote: ... which was conducted with the “Make Your Own “Mate & Plate™” Library System” according to the manufacturer’s instructions with minor modifications (Clontech). Oligo-dT primers provided in the kit were used for first-strand cDNA synthesis with 2 µg total RNA as input ...
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bioRxiv - Cancer Biology 2022Quote: ... 4×106 B cells per well were plated in 2 mL on 6-well plates that had been coated with RetroNectin (25 μg/mL, 4°C, overnight; #T100B, Takara), blocked with 2% BSA in PBS (1h ...
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bioRxiv - Neuroscience 2023Quote: ... and AAV1-syn-F14F15S-sTpEptTA_v2 were made by transfecting 8×15c plates (per virus) of HEK 293T cells using Xfect transfection reagent (Takara 631318). Briefly ...
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Induction of PARP7 Creates a Vulnerability for Growth Inhibition by RBN2397 in Prostate Cancer CellsbioRxiv - Cancer Biology 2023Quote: ... BBQ and FICZ as indicated at 37°C in 96-well plates for 5 days prior to measuring viability using WST reagent (Takara) following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Libraries were harvested by scraping colonies from transformation plates and directly purifying plasmids (with no outgrowth that could introduce amplification bias) using Nucleobond Xtra midiprep kits (Takara).
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bioRxiv - Microbiology 2024Quote: ... Cells were washed twice and single rEtpA-binding PBs (rEtpA+ CXCR5low CD71+ CD20low CD38+ IgDlow CD19+/int CD4− CD8− CD14− live singlet lymphocytes) were sorted using a Bigfoot into 96-well plates containing 2 µL Lysis Buffer (Clontech) supplemented with 1 U/µL rNase inhibitor (NEB ...
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bioRxiv - Microbiology 2024Quote: ... Cells were washed twice and single PBs (CD138+ CD38lo CD19+/lo CD3- live singlet lymphocytes) were sorted using a FACSAria II into 96-well plates containing 2 µL Lysis Buffer (Clontech) supplemented with 1 U/µL rNase inhibitor (NEB ...
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bioRxiv - Microbiology 2024Quote: ... lytic-induced or uninduced cells (3.5 × 105 cells in a 6-well plate) were harvested with 500 μL of RNAiso Plus (Takara Bio). The extracted total RNA was treated with DNase I (New England Biolabs ...
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bioRxiv - Microbiology 2024Quote: ... Dropout medium and plates were prepared with DifcoTM Yeast Nitrogen Base without Amino Acid (Becton Dickinson) and DO supplements (Clontech) following the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... Retroviral supernatants were then spun for 1 h at 3100g at 4 °C in 12-well-plates coated with 32 mg/ml RetroNectin (Takara). Meanwhile ...
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bioRxiv - Plant Biology 2024Quote: ... the Y2HGold strain was transformed with the bait vector (pGBKT7- RS2Z32 or pGBKT7-RS2Z33) and then mated with strain Y187 containing an Arabidopsis cDNA library (Mate and Plate Library-Universal Arabidopsis, Clontech) or a custom cDNA library made from entire mature leaf material (Make Your Own “Mate & Plate” Library System ...
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bioRxiv - Immunology 2024Quote: ... The individual 25 µl volume 5’RACE PCR reactions were then carried out in 96-well plates using the thermocycler program recommended by the 5’RACE kit (Takara), with 35 cycles of gene-specific amplification with an annealing temperature of 60°C ...
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bioRxiv - Cancer Biology 2024Quote: ... non-tissue culture treated 12-well plates were coated overnight at 4°C with 25 μg/ml retronectin in PBS (Takara). Plates were washed with PBS and blocked with PBS supplemented with 2% BSA for 15 min ...
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bioRxiv - Cancer Biology 2021Quote: ... Retroviruses encoding a second-generation CAR targeting HER-2 was obtained from the supernatant of the GP+E86 packaging line spun together with T cells onto RetroNectin-coated (10 μg/ml) 6-well plates (Takara Bio) and incubated overnight before the second viral transduction ...
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bioRxiv - Cancer Biology 2021Quote: ... freshly harvested single cells were sorted into 96-well plates containing the lysis buffer (0.2% triton-X-100, 1U/µl RNase inhibitor (Takara Bio, 2313A). Reverse transcription was performed using SuperScript II (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... qPCR experiments were performed in 72-well PCR plates using SYBR® Premix Ex TaqTMII-Tli RNaseH Plus (Takara, Dalian, China) in a 20-μL final reaction volume ...
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bioRxiv - Cell Biology 2022Quote: Cells cultured in either 12- or 24-well plates were washed twice with cold PBS and harvested using a TaKaRa MiniBEST universal RNA extraction kit (Takara, Japan). RNA was purified using the same kit according to the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Cells cultured in either 12 or 24-well plates were washed twice with cold PBS and harvested using TaKaRa MiniBEST Universal RNA extraction kit (Takara, Japan). RNA was purified using the same kit according to manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... for 24 h and transduced with lentiviral particles by spinfection (1000 x g for 90 min at 32°C) in the presence of Polybrene (5 μg/ml) on the plates coated with Retronectin (50 μg/ml) (Takara/Clontech) and anti-CD3 (1–2 μg/ml) ...
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bioRxiv - Immunology 2021Quote: ... Aliquots of 1 Mio cells in 1 ml medium were grown overnight in 12- or 24-well plates (either TC-treated or coated with 5 µg/cm2 retronectin [Takara Bio]) and then transduced with VSV-pseudotyped lentivirus encoding for either the 1G4 or the A6 TCR ...
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bioRxiv - Immunology 2020Quote: ... Aliquots of 1 Mio cells in 1 ml medium were grown overnight in 12-or 24-well plates (either TC-treated or coated with 5 μg/cm2 retronectin [Takara Bio]) and then transduced with VSV-pseudotyped lentivirus encoding for either the 1G4 or the A6 TCR ...
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bioRxiv - Microbiology 2021Quote: ... were transduced into TCR-deficient Jurkat cells or primary T-cells using the Plate-GP and PG13 cell-based retrovirus system and Retronectin (Takara Bio) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... activated CD8+ T cells were transduced with MSCV-ASNS-OE or MSCV-EV viral particles on RetroNectin-coated plates (Takara Bio). 24 hours later ...
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bioRxiv - Microbiology 2023Quote: ... lytic-induced or uninduced cells (5×105 cells on a 6-well plate) were harvested with 500 μL of RNAiso Plus (Takara Bio). Total RNA was extracted ...
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bioRxiv - Developmental Biology 2023Quote: ... Sorted cells (>50,000 cells) were cultured on 48-well tissue culture plates coated with RetroNectin (r-fibronectin CH-296; Takara Bio Inc.) in EC media ...
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bioRxiv - Cell Biology 2023Quote: ... suspensions of the transformants were spotted onto SD plates with dropout supplements: Leu/-Trp (cat #630417) or -Ade/-His/-Leu/-Trp (cat #630428) (Takara Bio). The strains used in this assay are listed in Table S1.
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bioRxiv - Cell Biology 2023Quote: ... Single cells were sorted by FACS as described above into 96-well plates containing 0.1 μl of RNase inhibitor (Clontech, Cat# 2313A), 1.9 μl of 0.2% Triton X-100 (Sigma ...
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bioRxiv - Immunology 2023Quote: ... Plates were coated with 5μg/mL of target peptide using coating reagent from the Takara Peptide Coating Kit (Takara cat. #MK100). Measles peptide was utilized as a negative control ...
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bioRxiv - Developmental Biology 2024Quote: ... for 20 minutes on ice and then dispensed into the nano-well plates of the ICELL8® cx Single-Cell System (Takara). Wells containing single viable cells were automatically selected using the ICELL8 cx CellSelect v2.5 Software (Takara ...
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bioRxiv - Immunology 2023Quote: ... Retroviral supernatants were then collected and spun at 2,000g for 2 h at 32 °C onto 24-well non-tissue-culture-treated plates coated overnight in Retronectin (Takara Bio) or spinfected for 90 minutes at 32°C in 1 µcg mL-1 polybrene ...
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bioRxiv - Cancer Biology 2023Quote: Reverse Transcriptase PCR and Real-Time PCR Cells cultured in either 12- or 24-well plates were washed twice with cold PBS and harvested using a TaKaRa MiniBEST universal RNA extraction kit (Takara, Japan). RNA was purified using the same kit according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2023Quote: ... Activated T cells were transduced on day 1 after stimulation using combinations of PEG-precipitated retroviral concentrates encoding different constructs adsorbed onto non-tissue culture treated 6-well plates coated with anti-CD3/CD28 2 μg/mL each and retronectin 20 μg/mL (Takara, T100B) at 1-2×106 cells/well ...
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bioRxiv - Developmental Biology 2023Quote: ... The plate containing dried beads bound to single-cell DNA was extracted from the robot and processed following PicoPLEX® (Sopachem/Takara) protocol with half volumes as described in Macaulay et ...
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bioRxiv - Biochemistry 2024Quote: ... plates with 40 μg/ml X-α-Gal (FUJIFILM Wako Pure Chemical, Osaka, Japan) and 200 ng/ml aureobasidin A (TaKaRa) (QDOGA ...