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Citations for Takara Bio :
2301 - 2350 of 4951 citations for SpectraDye Antibody Labeling Kit IR700 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... The reaction solution was prepared according to the instructions of the reverse transcription kit (Takara) to reverse-transcribe RNA to cDNA ...
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bioRxiv - Microbiology 2020Quote: ... followed by a brief centrifugation step before being resolved by SDS-PAGE and subjected to western blotting using anti-6xhis antibodies (TaKaRa: 631212), or anti-BamA POTRA antiserum (36) ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated with a mix of up to three primary antibodies simultaneously diluted in PBST with 1 % NDS overnight at room temperature with the following primary antibodies: rabbit anti-dsRed (1:1000; Clontech, AB_10013483), chicken anti-GFP (1:1000 ...
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bioRxiv - Molecular Biology 2020Quote: ... The relative luciferase activity was determined by normalizing the firefly luciferase activity by the respective total EGFP protein amount quantified by Western blot using a GFP antibody (Clontech, 632460) in Image Studio ver 5.2 (LI-COR).
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bioRxiv - Microbiology 2019Quote: ... Samples were boiled for 10 min before being resolved by SDS-PAGE and subjected to western blotting using anti-6xhis antibodies (TaKaRa: 631212) as primary antibody and HRP conjugated anti-rabbit (Sigma Aldrich ...
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bioRxiv - Bioengineering 2019Quote: ... MO). The horseradish peroxidase (HRP)-conjugated monoclonal anti-His antibody (anti-mouse Cat. #631210) was obtained from Clontech (Mountain View, CA). The ultrasensitive HRP substrate used for Western blotting was from TaKaRa (Shiga ...
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bioRxiv - Plant Biology 2021Quote: ... Membranes were stained with Ponceau S solution and then incubated with anti-GFP antibody (JL-8 Takara Bio Clontech, Lot #A8034133).
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bioRxiv - Plant Biology 2021Quote: ... Membranes were stained with Ponceau S solution and then incubated with anti-GFP antibody (JL-8 Takara Bio Clontech, Lot #A8034133).
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bioRxiv - Neuroscience 2022Quote: ... protein lysate was immunoprecipitated for 2 h at 4°C with the following antibodies: mouse anti-GFP (1:1000; Takara Bio), mouse anti-FLAG (1:1000 ...
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Orchestration of alternative splicing regulates bone marrow mesenchymal stem cells fate during agingbioRxiv - Cell Biology 2022Quote: ... bone sections were blocked in 5% bovine serum albumin (BSA) for 1 hour at room temperature and incubated with primary antibody to osteocalcin (Takara, M173) at 4°C overnight ...
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bioRxiv - Neuroscience 2022Quote: Immunohistochemistry of spinal cord cryostat sections (30µm) was performed using the following primary antibodies: Rabbit anti-DsRed (1:3000, Takara Bio, 632496), guinea pig anti-VGLUT1 (1:8000 ...
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bioRxiv - Plant Biology 2019Quote: ... western blot and immuno-detection using monoclonal antibodies raised in mouse against the GAL4 activation or binding domain (1:10000 dilution, Clontech, www.clontech.com) following the Matchmaker Monoclonal Antibodies User Manuel from Clontech ...
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bioRxiv - Neuroscience 2019Quote: ... and then in primary antibodies in PBST at 4°C for 48 hours using rabbit anti-DsRed (mCherry tag; 1:500; Clontech; 632496), and mouse anti-Th (1:1,000 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Membrane was blocked overnight in 5% milk-TBS + 1% Tween and incubated the next day with a primary antibody directed against the Gal4-activation domain (1:5,000 dilution; Clontech, cat # 630402) or against human Ku70p (1:1,000 dilution ...
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bioRxiv - Biochemistry 2023Quote: ... The cells were fixed with ice-cold 4 % paraformaldehyde for 20 min and stained with rabbit anti-dsRed antibody (Takara Bio) and Alexa fluor 568-conjugated anti-rabbit secondary antibody to identify cells expressing mApple-tagged Myo6 ...
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bioRxiv - Cell Biology 2022Quote: ... were run onto 8% SDS- polyacrylamide gels that were transferred to nitrocellulose membranes which were reacted with α-His tag antibody (#631212, Clontech). Quantitation of band intensities was done with ImageLab software (BioRad) ...
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bioRxiv - Neuroscience 2023Quote: ... The expression of GFP- or Venus-tagged constructs was measured by Western blot with mouse anti-GFP antibody (Clontech; 1:2,000). The expression of WT and mutant arrestin-3 was measured with rabbit polyclonal anti-arrestin-3 antibody ...
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bioRxiv - Neuroscience 2023Quote: ... They were then incubated overnight at 4°C with the following primary antibodies diluted in PBS - 0.3% Triton X100 - 1% NGS: rabbit anti-ZsGreen (1/1000, Clontech, 632474, RRID:AB_2491179) or rabbit anti- DCX (1/2000 ...
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bioRxiv - Neuroscience 2023Quote: ... in PBS and then incubated overnight with gentle agitation at 4 °C in blocking solution plus 1:1000 dilution primary antibody (chicken anti-GFP polyclonal, Abcam; rabbit anti-dsRed polyclonal, Takara Bio). Sections were then incubated covered with gentle agitation for 2 hours at room temperature in blocking solution plus 1:500 dilution secondary antibody (goat anti-rabbit IgG Alexafluor 594 conjugate ...
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bioRxiv - Developmental Biology 2023Quote: ... The following antibodies were used: rabbit or rat anti-dsRed (1:200, Clontech 632496 or 5F8 1:400, Chromotek 5f8-100); chicken anti-GFP (1:800 ...
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bioRxiv - Cell Biology 2024Quote: For immunostaining the following antibodies were used: mouse anti-E-cadherin mAb clones SHE78-7 and HECD1 (Takara, M126 and M106), mouse anti-vinculin (Sigma ...
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bioRxiv - Plant Biology 2020Quote: ... and cDNA synthesis was performed by using the PrimeScriptTM 1st strand cDNA synthesis kit (TAKARA, Japan). Target genes were amplified with the following cycling programme ...
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bioRxiv - Microbiology 2020Quote: ... The synthesized cDNA was purified using a NucleoSpin Gel and PCR Clean-up Kit (Takara Bio) and eluted with 30 μl of 5 mM Tris-HCl buffer (pH 8.5) ...
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bioRxiv - Microbiology 2020Quote: ... before cDNA was synthesized using the PrimeScript RT reagent kit with gDNA Eraser (Takara, Beijing, China) following the protocols provided by the manufacturer ...
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bioRxiv - Cancer Biology 2021Quote: ... and TCR beta chain libraries were generated using SMARTer Mouse TCR a/b Profiling Kit (Clontech). Samples were pooled to a final pool concentration of 4 nM and diluted to a final concentration of 13.5 pM ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was amplified with a SYBR Green dye kit and gene-specific primers (Takara, Shiga, Japan). Data were normalized to β-actin mRNA levels ...
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bioRxiv - Cell Biology 2020Quote: ... was performed using the In-Fusion Cloning Kit and online tools (BD Clontech, Takara Bio, USA). All plasmids were verified by sequencing (Genewiz ...
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bioRxiv - Cell Biology 2020Quote: ... RNA-seq libraries were created using the SMARTer Stranded Total RNA Sample Prep Kit (Takara Bio) and sequenced on the NextSeq 500 (Illumina ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA-seq libraries were created using the SMARTer Stranded Total RNA Sample Prep Kit (Takara Bio) and sequenced using NextSeq500/550 High Output v2.5 kit (spiked with 10% PhiX ...
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bioRxiv - Microbiology 2021Quote: ... Products from these two reactions were ligated via In-Fusion HD Cloning Kit (Clontech®, 639650). RSV-GagΔNC (Volker Vogt ...
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bioRxiv - Molecular Biology 2020Quote: ... containing the beta-globin (HBB) 5’-UTR using the In-Fusion® HD Cloning Kit (Takara). The subsequent mutations in the TCRA and TCRB 3’-UTRs were generated using these initial constructs ...
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bioRxiv - Immunology 2021Quote: ... and the protein concentration was measured by BCA protein assay kit (TaKaRa, Dalian, China, cat#T9300A) as previous described(Ma et al. ...
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bioRxiv - Microbiology 2019Quote: ... and 5’- or 3’-RACE was performed with SMARTer®RACE 5’/3’ Kit (Takara, 634858) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: RNAi plasmids were constructed using the In-Fusion® HD Cloning Kit (Takara Bio; Cat #: 639650) and the L4440 vector ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA was generated using the Smart-seq ultra-Low input RNA kit (Takara Bio USA, Inc.). cDNA quality control was assessed using a TapeStation 2200 system (Agilent Technologies ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Libraries were prepared using the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Clontech) and sequenced on an Illumina NextSeq500 at The University of Glasgow Polyomics Facility ...
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bioRxiv - Developmental Biology 2020Quote: ... cDNA synthesis was performed using a PrimerScriptTM RT reagent Kit with gDNA Eraser (TaKaRa, Lot # AJ51485A) according to the manufacturer’s instruction ...
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bioRxiv - Developmental Biology 2021Quote: ... a library was prepared using the SMART-seq v4 ultra low input RNA kit (Takara Bio). Sequencing was performed by HiSeq 2500 (Illumina ...
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bioRxiv - Developmental Biology 2021Quote: ... These polyA sequencing libraries were generated using SMARTer Ultra Low Input RNA HV kit (Clontech, USA) using the manufacturer’s recommended protocol ...
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bioRxiv - Physiology 2022Quote: ... LV particles were concentrated from the filtered supernatants using a Lenti-X concentrator kit (Takara, 631232) following manufacturer’s protocol.
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bioRxiv - Microbiology 2022Quote: ... Linearized vector and inserts were ligated using In-Fusion® HD Cloning Kit (Clontech, Takara, Japan) and transformed into StellarTM competent cells ...
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bioRxiv - Neuroscience 2021Quote: ... Reverse transcription for quantitative PCR (qPCR) analyses was performed with PrimeScript RT Master Mix kit (Takara). qPCR was performed with TransStart Top Green qPCR SuperMix kit (TransGen).
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bioRxiv - Neuroscience 2021Quote: ... Reverse transcription for cDNA cloning was performed with PrimeScriptTM II 1st Strand cDNA Synthesis kit (Takara), Gibson assembly was performed with NEBuilder HiFi DNA
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bioRxiv - Neuroscience 2021Quote: ... and the AAV was purified using AAVpro Purification Kit Maxi (All Serotypes) (no. 6666, Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... DNA was prepared using the endotoxin-free NucleoBond Xtra Maxi EF kit (Clontech, Takara Bio, USA).
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bioRxiv - Neuroscience 2021Quote: ... A cDNA library was created with the CellAmp™ Whole Transcriptome Amplification Kit (#3734, Takara Bio) to allow for real-time PCR (qPCR ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA (10 ng) was extracted for library preparation using a SMART-Seq Stranded Kit (Takara) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... The digested PCR product was cloned into a TA-vector by Mighty TA-cloning Kit (Takara). After LacZ selection ...
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bioRxiv - Genomics 2021Quote: ... barcoded cDNA products from nanowells were pooled by means of the SMARTer ICELL8 Collection Kit (Takara). cDNA was concentrated using the Zymo DNA Clean & Concentrator kit (Zymo Research ...
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bioRxiv - Genomics 2020Quote: ... 500-1000ng of total RNA was reverse transcribed using the Advantage RT-for-PCR Kit (Takara) and qPCR performed in quadruplet using SYBR Green PCR Master Mix (Thermo Fisher Scientific ...