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Citations for Takara Bio :
2301 - 2350 of 5557 citations for Human E3 Ubiquitin Protein Ligase SMURF2 SMURF2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... we performed RACE PCR using a SMARTer® RACE 5’/3’ Kit (Takara Bio), according to the manufacturer’s specifications ...
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bioRxiv - Biochemistry 2020Quote: ... The product was cloned into pET28a plasmid using In-Fusion PCR Cloning Kit (Clontech), with an N-terminal hexahistidine (His6 ...
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bioRxiv - Microbiology 2021Quote: ... Total RNA was extracted employing the Takara MiniBEST Universal RNA Extraction Kit (Takara, Japan) and reverse transcription was conducted employing the PrimeScript RT Master Mix (Takara ...
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bioRxiv - Bioengineering 2020Quote: ... Then qPCR was conducted with a One-Step PrimeScrip RT-PCR Kit (Takara, Japan), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... and purified with NucleoSpin Gel and PCR Clean-up kit (Takara, Cat. No. 74061) for Sanger sequencing ...
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bioRxiv - Neuroscience 2022Quote: ... The titer was measured using a Lenti-X qRT-PCR titration kit (Clontech 631235).
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bioRxiv - Cancer Biology 2022Quote: ... to yield pMXs-Neo-hMPI by using In-Fusion HD Cloning Kit (639648, Clontech), in accordance with the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... The titer of retrovirus was measure by Retro-X qRT-PCR Titration Kit (Clontech). For iPSC generation ...
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bioRxiv - Genomics 2022Quote: ... and cDNAs were generated with a PrimeScript RT Reagent Kit with gDNA Eraser (Takara). We performed qRT–PCR with TB Green Premix Ex Taq II (Takara) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The ΔMDH mutant was generated via PrimeStart® Max mutagenesis kit (TaKaRa Bio Inc), using primers 5’-GCGCTGGCGCTGCTGCTG and 3’-CAGCAGCGCCAGCGCGCCAATGCCCGCGCCCAGGTTCGCC ...
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bioRxiv - Cell Biology 2022Quote: ... The reverse transcription reaction was performed using ExScript RT Reagent Kit (Takara, Tokyo, Japan). The primers used in this study were as follows:
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bioRxiv - Genetics 2022Quote: ... Physical titer was determined using the Lenti-X p24 Rapid Titer Kit (Takara, 632200) and granule cell precursors were transduced at an estimated MOI of ∼4.
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bioRxiv - Physiology 2022Quote: ... cDNA synthesis was performed using the Takara SMART-seq v4 PLUS kit (Takara, #634889) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... Resulting vector and gene fragment were combined via Gibson Assembly (Takara In-fusion Kit) generating pLew100-FLPOLIBRc-PTPPuro ...
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bioRxiv - Neuroscience 2022Quote: ... with the mCherry sequence by the In-Fusion HD Cloning Plus kit (Takara, 638910). For the transposon-based p53 reporter assay performed on KOLF2.1J ...
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bioRxiv - Synthetic Biology 2019Quote: ... cDNA was generated using the PrimeScript™ RT reagent Kit with gDNA Eraser (TaKaRa). Real-time PCR was performed using SYBR Premix Ex Taq™ II (Tli RNaseH Plus ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and reverse-transcribed using PrimeScript™ RT reagent kit (Takara BIO Inc., Code No.RR047A) according to manufacturer’s instructions.
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bioRxiv - Genomics 2020Quote: ... cDNA was synthesized from total RNA using a cDNA Synthesis Kit (TAKARA BIO INC). The cDNA of all target genes was amplified for 50 cycles and quantified with a SYBRGreen-based real-time PCR (Applied Biosystems ...
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bioRxiv - Immunology 2019Quote: ... Multiplexed pair-end libraries 50nt in length were obtained using Nextera XT kit (Clontech). Sequencing was performed in a single batch with Illumina HiSeq 2500 using an average depth of 15 million reads ...
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bioRxiv - Immunology 2020Quote: ... QPCR was performed for triplicate samples using a SYBR Green qPCR Kit (Takara, JPN) by the Applied Biosystems™ QuantStudio™ 6 standard Real-Time PCR System (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2019Quote: ... cDNAs were synthesized using the PrimeScript™ RT reagent Kit with gDNA Eraser (TaKaRa) following standard protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... qPCR was performed with a StepOnePlus™ Real-Time PCR Systems from Applied Biosystems and the SYBR Green PCR Core reagents kit (Takara, Ozyme, Saint Cyr L’Ecole, France). All RNAs for the miRNA investigation were transcribed using miScript RT kits and subsequent qPCR performed using miScript Primer Assays and miScript SYBR kits (Qiagen ...
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bioRxiv - Genetics 2019Quote: Viral RNA was extracted from cell suspensions using a Viral RNA Extraction Kit (TaKaRa) and following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2019Quote: ... The DNA libraries were prepared using a SMARTer ThruPLEX Tag-seq Kit (Takara Bio), and the samples were sequenced on an Illumina HiSeq1500 system.
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bioRxiv - Genomics 2019Quote: ... Ribosomal depletion was performed with the RiboGone-Mammalian kit (Clontech Cat. Nos. 634846 & 634847) and cDNA was created with the SMARTer Stranded RNAseq Kit (Cat ...
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bioRxiv - Plant Biology 2019Quote: ... Purified RNA was reversed transcribed by Prime Script RT reagent kit (TaKaRa, Dalian, China). The cDNA was used for the templates of RT-PCR and quantitative RT-PCR ...
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bioRxiv - Plant Biology 2020Quote: ... The cDNA was synthesized using a PrimeScript RT reagent kit with gDNA Eraser (TaKaRa); 5x diluted cDNA served as the template ...
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bioRxiv - Cancer Biology 2019Quote: ... Real-Time PCR was performed using SYBR Premix EX Taq kit (Takara, Shiga, Japan). The specific primers were used to detect the relative mRNA expression of IL-1RA by the 2 - ct method ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Libraries were prepared with SMARTer universal low input RNA kit (Takara, Mountain View, CA) following ribosome depletion ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... The cDNA was synthesized by using a PrimeScript™ RT reagent Kit (Takara, China) following the manufacturer’s instructions ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... by using SYBR Premix Ex Taq II (Tli RNaseH Plus) Kit (Takara, Dalian, China) and CFX96TM real-time system (Bio-Rad ...
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bioRxiv - Cell Biology 2019Quote: ... and 500 ng RNA used to synthesize cDNA using PrimeScriptTM RT reagent kit (TaKaRa) according to the manufacturer’s instructions ...
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Di-Ras2 Promotes Renal Cell Carcinoma Formation by Activating the MAPK Pathway in the Absence of VHLbioRxiv - Cancer Biology 2019Quote: ... cDNA was synthesized by reverse transcription using the Prime Script RT reagent kit (TaKaRa) and subjected to quantitative RT-PCR with Di-Ras2 ...
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bioRxiv - Molecular Biology 2020Quote: ... Complementary DNA (cDNA) was synthesized with the PrimeScript™ RT reagent Kit (Takara, RR047A) involved in genomic DNA erasing ...
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bioRxiv - Developmental Biology 2020Quote: ... The PCR reaction system was prepared with a kit (SYBR Premix Ex Taq, Takara), and the reaction was performed on an Mx3000P real-time PCR machine (Agilent Stratagene ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA was synthesized with the Clontech SMARTer PCR cDNA Synthesis Kit (Takara Bio Inc). RNA was also extracted from dissected brains of one-day old paralarvae in a similar manner and cDNA was synthesized using the NEBNext cDNA Synthesis & amplification kit ...
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bioRxiv - Molecular Biology 2021Quote: ... 5’ RACE cDNA library was prepared using Clontech SMARTer RACE 5’/3’ Kit (Takara) according to the user manual with the gene-specific primer (S4 Table ...
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bioRxiv - Cancer Biology 2021Quote: ... cDNA was synthesized from 500 ng RNA using PrimeScript RT Reagent Kit (Takara, RR037). A reaction mixture was prepared by mixing 50 ng gDNA or cDNA templates ...
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bioRxiv - Genomics 2020Quote: ... followed by library preparation using the SMARTer Stranded Total RNA-seq kit v2 (Takara) according to the manufacturer’s protocols.
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bioRxiv - Molecular Biology 2021Quote: ... and cDNA was synthesized using the PrimeScript RT reagent kit with gDNA eraser (TaKaRa). A random hexamer was used for cDNA synthesis ...
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bioRxiv - Microbiology 2020Quote: Total RNA was extracted using a Nucleospin RNA XS kit (Takara Bio, Kusatsu, Japan). Evaluation of RNA ...
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bioRxiv - Microbiology 2021Quote: ... The reverse transcription of RNA into cDNA using PrimeScript RT reagent kit (TaKaRa, China). Subsequently ...
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bioRxiv - Zoology 2020Quote: ... the in vitro Transcription T7 Kit (for siRNA Synthesis) (Takara Bio Inc., Shiga, Japan) was used.
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bioRxiv - Neuroscience 2019Quote: ... or IMPA1L 120nt fragment were labeled using Random Priming Labeling Kits (Takara or Roche) and [α−32P] dCTP ...
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bioRxiv - Microbiology 2020Quote: Genomic DNA was extracted by using a bacterial genomic DNA Mini kit (TaKaRa Bio) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: ... cDNA was synthesised using PrimeScript™ RT Reagent Kit (Takara Bio Inc., Shiga, Japan), and the mRNA content was quantified by real-time PCR using the LightCycler® 96 System (Roche Diagnostics K.K. ...
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bioRxiv - Immunology 2020Quote: ... Libraries were prepared using the SMART-Seq v4 Ultra Low Input RNA Kit (Takara). PCR products were then indexed using a Nextera XT DNA Library Prep Kit (Illumina ...
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bioRxiv - Genomics 2019Quote: ... and then reverse-transcribed into cDNA using a PrimeScript RT reagent kit (TaKaRa, RR037A). Real-time PCR was performed on LightCycler 96 (Roche) ...
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bioRxiv - Molecular Biology 2019Quote: ... All constructions were obtained by assembling PCR fragments using InFusion® cloning kit (Takara). Using appropriate sets of oligonucleotides ...
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bioRxiv - Genetics 2020Quote: ... and SMART-Seq™ v4 Ultra™ Low Input RNA Kit for Sequencing (Clontech) was used for cell lysis and first strand cDNA synthesis ...