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Citations for Takara Bio :
2201 - 2250 of 3888 citations for Recombinant Mouse Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... and or DsRed (1/1000, Takara Bio, lot #2103116) in a solution containing 0.5% Triton-X100 ...
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bioRxiv - Cell Biology 2023Quote: ... anti-human specific GFAP (hGFAP, TAKARA, Stem123, 1:1000), anti-AQP4 (Cell Signaling ...
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bioRxiv - Molecular Biology 2023Quote: ... The medium was exchanged to CELLBANKER 1 plus (TaKaRa) and stored at −80°C ...
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Brief Change in Dopamine Activity during Consolidation Impairs Long-Term Memory via Sleep DisruptionbioRxiv - Neuroscience 2023Quote: ... and rabbit anti-DsRed (1:200, Cat# 632496, Clontech). Secondary antibodies were Alexa Fluor 488 (goat anti-chicken ...
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Brief Change in Dopamine Activity during Consolidation Impairs Long-Term Memory via Sleep DisruptionbioRxiv - Neuroscience 2023Quote: ... and rabbit anti-DsRed (1:200, Cat# 632496, Clontech); primary antibodies used to detect GRASP signals was mouse monoclonal anti-GFP (1:200 ...
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bioRxiv - Neuroscience 2023Quote: ... Ds-red polyclonal anti-rabbit (1:200; Takara, 632496). C1q monoclonal anti-rabbit (1:1000 ...
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bioRxiv - Plant Biology 2024Quote: ... following standard procedures (Yeast Protocols Handbook PT3024-1, Clontech). The proteins were extracted by post-alkaline methods59 and detected by western blot with antibody Myc (Abmart ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 1×SYBR Green Master Mix buffer (Takara, Otsu, Japan) was used for quantitative RT-PCR and assays were performed on a Roche lightCycler 480 II PCR machine ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibody Rabbit-anti-DsRed (Clontech #632496, 1:1000) and secondary antibody HRP-goat-anti-rabbit (1:500 ...
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bioRxiv - Biochemistry 2021Quote: DNA encoding the PX domain from the Qc SNARE Vam7 (Amino acyl residues 2-123) was amplified by PCR with CloneAMP HiFi PCR premix (Takara Bio USA, Mountain View, CA, USA). The amplified DNA fragment was cloned into BamHI and SalI digested pGST parallel1 vector (Sheffield et al.,1999 ...
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bioRxiv - Cell Biology 2022Quote: ... while pGADT7-CmVPS41s and pGADT7-T control prey plasmids were transformed into yeast strain Y187 following the Yeastmaker Yeast Transformation System 2 instructions (Clontech by Takara Bio, Mountain View, USA). The transformed cells were grown either in SD-Trp agar plates for Y2HGold or in SD-Leu agar plates for Y187 at 30 ºC for 3-5 days ...
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bioRxiv - Evolutionary Biology 2022Quote: ... with 0.1 µL of Taq Polymerase 5 U.µL-1 (TaKaRa Ex Taq® Kit MgCl2 Free Buffer) (TaKaRa Ex Taq, TaKaRa Bio, Shiga, Japan), 2.5 µL PCR Buffer 10X ...
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bioRxiv - Neuroscience 2023Quote: ... the samples were incubated in primary antibody (chicken anti-GFP, Abcam #13970, 1:2000, rabbit anti-DsRed, Takara Bio Clontech #632496, 1:200) for 24 – 48 hours in the dark while shaking at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... the samples were incubated in primary antibody (chicken anti-GFP, Abcam #13970, 1:2000, rabbit anti-DsRed, Takara Bio Clontech #632496, 1:200) for 24 – 48 hours in the dark while shaking at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... Tissues were rinsed four times for 15 min each with 0.1 M PBS and incubated with secondary antibodies anti-DsRed (1:500; RRID:AB_10013483; Living Colors DsRed polyclonal; Takara Bio USA, San Jose, CA) and anti-Troma1 (1:500 ...
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bioRxiv - Bioengineering 2023Quote: ... the input binary libraries (1:10 or 1:100) and the selected pools were subjected to restriction digestion using BamHI (Takara Bio Inc., Japan). The digestion products were then analyzed using a 10% polyacrylamide gel electrophoresis (PAGE ...
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bioRxiv - Cell Biology 2020Quote: ... total RNA was extracted from cells using RNAiso (Takara Bio, USA) according to manufactures protocol ...
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bioRxiv - Genetics 2021Quote: ... The assembly reaction was then transformed into Stellar competent cells (Takara), and following an hour-long outgrowth ...
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bioRxiv - Cell Biology 2020Quote: ... The ligated plasmid was then transformed into Stellar Competent cells (Clontech), and streaked onto LB agar plates ...
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bioRxiv - Microbiology 2021Quote: ... and the resulting plasmid was transformed into Stellar competent cells (Takara).
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bioRxiv - Cancer Biology 2022Quote: ... MDA-MB-231 (ATCC) and lenti-X 293T cells (Takara Bio) were grown in DMEM (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... cells were treated with 100ug/mL hygromycin B (Takara bio 631309). Cells were exposed to 100ug/mL hygromycin B for 8 days ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were transfected with the LentiX VSV-G transfection mix (Clontech) and different SARS-CoV-2 ORFs (cloned into pLX vector) ...
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bioRxiv - Molecular Biology 2021Quote: H4 cells (ATCC, HTB-148) stably expressing the pIRESpuro3 vector (Clontech) containing a codon-optimized 0N4R MAPT gene with the P301S point mutation and tagged with YFP were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Molecular Biology 2020Quote: ... Viral supernatants were obtained from Lenti-X 293T cells (Clontech #632180) transfected with either pInducer21 GFP lentiviral vector or pInducer21 GFP constructs containing either p42 ...
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bioRxiv - Molecular Biology 2021Quote: ... instructions and reactions were transformed into Stellar Competent Cells (Takara 636766).
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bioRxiv - Plant Biology 2019Quote: ... was co-transformed into chemically competent Y2HGold cells (Takara Bio, USA) with the individual AVR-Pik variants or mutants in pGADT7 described previously (22) ...
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bioRxiv - Genomics 2019Quote: The SMARTer ICELL8 single-cell system (Takara Bio USA, Cat. #640000) was used to array single cells as described for scATAC-seq12 ...
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bioRxiv - Genomics 2020Quote: The SMARTer ICELL8 single-cell system (Takara Bio USA, Cat. #640000) was used to array single cells previously described3 ...
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bioRxiv - Neuroscience 2021Quote: ... was transformed into chemically-competent BL21 (DE3) Codon Plus cells (Clontech). A single colony was selected for growth in terrific broth (Fisher ...
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bioRxiv - Cell Biology 2021Quote: The Lenti X 293T cell line (632180) was purchased from Takara Bio ...
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Plant pathogens convergently evolved to counteract redundant nodes of an NLR immune receptor networkbioRxiv - Plant Biology 2021Quote: ... were co-transformed into chemically competent Y2HGold cells (Takara Bio, USA) with individual NRC truncates in pGADT7 (prey ...
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bioRxiv - Neuroscience 2020Quote: ... The cloning mixture was chemically transformed into Stellar Competent Cells (Clontech) and plated onto square agar plates for a total of ~10,000 colonies per round ...
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bioRxiv - Cancer Biology 2021Quote: Stable cells lines were created using the retroviral vector pQXCIP (Clontech). The cDNAs of ZsGreen ...
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bioRxiv - Microbiology 2022Quote: ... The human kidney epithelial cell line Lenti-X™ 293T (Takara) was cultivated in DMEM supplemented with 10 % (v/v ...
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bioRxiv - Cell Biology 2022Quote: ... E.coli competent cells transformed with pTf16 chaperone protein tag (TaKaRa Bio) per the manufacturer’s protocol.
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bioRxiv - Genetics 2022Quote: ... Lentiviral shRNA particles were prepared with Lenti-X 293T cells (Takara) and MISSION pLKO.1 plasmids (Sigma-Aldrich ...
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bioRxiv - Genomics 2022Quote: ... sgRNA constructs were amplified upon transformation of DH5α competent cells (Takara) grown at 37ºC ...
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bioRxiv - Biochemistry 2022Quote: ... HeLa cells were transfected with the pLVX-TetOn-Advanced plasmid (Clontech) and selected with 500 μg/mL G418 for 14 days ...
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bioRxiv - Neuroscience 2020Quote: Lentiviruses were produced by transfection of Lenti-X 293T cells (Takara) with a lentiviral plasmid containing EGFP ...
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bioRxiv - Genomics 2019Quote: Single-cell cDNA libraries were prepared using SMART-Seq v4 (Clontech) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2021Quote: ... Total RNA was extracted from the collected cells with nucleospin (Takara). Numbers of RNA copies of the virus were determined by quantitative real-time PCR (SARS-CoV-2 Detection Kit ...
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bioRxiv - Cancer Biology 2020Quote: ... HEK-LentiX cell line for lentivirus production was purchased from Takara Bio ...
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bioRxiv - Molecular Biology 2022Quote: ... Production of lentivirus was performed in Lenti-X 293T cells (Takara) by co-transection with pMD2.G ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... To produce the viruses HEK Lenti-X 293T cell line (Clontech) were transfected using PEI (Sigma ...
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bioRxiv - Cancer Biology 2022Quote: ... The Lenti-X™ 293T cell line was purchased from Takara-Bio (Cat ...
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bioRxiv - Cancer Biology 2022Quote: ... Enriched lentiviruses were infected into ATL cell lines using RetroNectin (TaKaRa) with spin infection (3,000rpm ...
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bioRxiv - Biochemistry 2020Quote: ... HEK293T cells were co-transfected with the construct and pPUR (Clontech) using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Cell Biology 2019Quote: ... Puromycin-resistant cells were then grown in Tetracycline-free serum (Clontech) and transduced with pLVX-Tet-On-Advanced lentivirus followed by G418 selection (500 μg/mL ...
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bioRxiv - Molecular Biology 2019Quote: ... These vectors were transfected in AmphoPack-293 cell line (631505, Clontech). Viral supernatants were harvested 48 h post transfection ...