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Citations for Takara Bio :
2051 - 2100 of 6571 citations for Mouse Interleukin 1 Family Member 10 IL1F10 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Lactylation-driven FTO-mediated m6A modification of CDK2 aggravates diabetic microvascular anomaliesbioRxiv - Cell Biology 2023Quote: ... cDNA was generated using a PrimeScript RT Kit (Takara, Otsu, Shiga, Japan). RNA levels were determined by qPCR with FastStart Universal SYBR Green Master (ROX ...
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bioRxiv - Cancer Biology 2023Quote: ... Complementary DNA synthesis was performed using PrimeScript RT Reagent Kit (Takara RR037A). The qPCR reactions were carried out using Power SYBR Green PCR Master Mix (Applied Biosystems 4367659 ...
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bioRxiv - Cancer Biology 2023Quote: ... using an In-Fusion HD Cloning Plus kit (Takara Bio, Shiga, Japan); (iv ...
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bioRxiv - Cancer Biology 2023Quote: ... libraries were prepared using the ThruPLEX®DNA-Seq kit (Takara, R400676) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... the plasmid was constructed using the In-Fusion HD Cloning Kit (Takara); 1–2 kb sequences of the 5’ and 3’ ends of the genes of interest flanked the fragment that consisted of an in-frame linker ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary hippocampal cultures were transfected using CalPhos mammalian transfection kit (Clontech, 631312) as previously (Jiang & Chen ...
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bioRxiv - Neuroscience 2023Quote: ... Subcloning was carried out using the In-Fusion cloning kit (Takara Bio). Endotoxin-free plasmids were purified using silica columns (NucleoBond Xtra Midi EF ...
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bioRxiv - Pathology 2023Quote: ... cDNA was prepared using PrimeScript cDNA synthesis kit (Takara, Cat No: 2680A) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... AAV2/9 particles were purified using the AAV Purification kit (Takara, Japan). The AAV solution was concentrated to the optimal volume by centrifugation using an Amicon Ultra-4 100k centrifugal filter unit (Millipore) ...
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bioRxiv - Molecular Biology 2024Quote: ... mRNA was reverse transcribed to cDNA with the PrimeScript RT kit (TaKaRa). cDNA was analysed by real-time PCR with the Light Cycler 480 SYBR Green I Master Mix (Roche) ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was synthetized using the PrimeScript™ RT Reagent Kit (Takara #RR037A) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... while cDNA insert was purified using NucleoSpin® Clean-up Kit (Takara). Insert generation was performed by High-Fidelity PCR using primers designed to yield fragments containing exons 1-10 or 12-16 with the necessary overlap ...
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bioRxiv - Cell Biology 2023Quote: ... digested with EcoRI using the In-Fusion HD Cloning Kit (Takara Bio). Then ...
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bioRxiv - Bioengineering 2023Quote: ... followed by cDNA synthesis using the cDNA Synthesis Kit (Cat# 6110A, Takara). All qRT-PCR primers were ordered from Eton Bioscience ...
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bioRxiv - Bioengineering 2023Quote: ... followed by RNA purification using NucleoSpin RNA kit (Takara Bio, Shiga, Japan) with on-column DNA digestion ...
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bioRxiv - Bioengineering 2023Quote: ... All cloning was performed using the In-Fusion Cloning kit (Takara Bio) and ligated plasmids were transformed into chemically competent Stbl3 E ...
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bioRxiv - Microbiology 2023Quote: ... HD In-Fusion kit (Takara 638909 In-Fusion® HD Cloning Plus) was used for cloning.
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bioRxiv - Cancer Biology 2023Quote: ... using CalPhos™ Mammalian Transfection Kit (Clontech, Inc., Mountain View, CA, USA), according to the manufacturer’s protocol as described (Wenta et al ...
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bioRxiv - Immunology 2024Quote: LDH in cell supernatants was measured using the LDH Cytotoxicity Kit (TaKaRa) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: Total RNA was extracted from monocytes using NucleoSpin® RNA kit (Takara) and RNA concentration was measured using NanoDrop Spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... The band was extracted and purified with Nucleospin Gel Extraction Kit (Takara). The gel-purified DNA was then further purified with Ampure XP beads (Beckman Coulter ...
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bioRxiv - Cell Biology 2023Quote: ... Ribosomal RNA was removed using RiboGone – Mammalian Kit protocol (Clontech, No. 634847). After rRNA depletion ...
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bioRxiv - Immunology 2024Quote: ... SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara Bio) was used for cDNA was synthesis ...
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bioRxiv - Neuroscience 2024Quote: ... QUAS-tdTomato-NLS plasmid was cloned using Infusion HD Cloning Kit (Takara) and sent to Genetivision Corporation for injection ...
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bioRxiv - Cell Biology 2024Quote: ... vector using In-Fusion® HD Cloning Kit (Takara Bio USA, Inc). Primer sequences used were as follows ...
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bioRxiv - Cell Biology 2024Quote: ... which was reverse transcribed using the PrimerScriptTM RT reagent Kit (TaKaRa, RR037A) to obtain cDNA ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was synthesized using a PrimeScript reverse transcriptase reagent kit (Takara, Japan). mRNA expression was examined by quantitative real-time PCR using iTaq Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Cancer Biology 2024Quote: ... Plasmids were prepped using the NucleoBond Xtra Midi Plus kit (Takara Bio).
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bioRxiv - Developmental Biology 2024Quote: ... single-strand cDNA generated with the cDNA synthesis kit (Takara, Otsu, Japan) was performed ...
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bioRxiv - Microbiology 2024Quote: ... Using the PrimeScript first strand cDNA synthesis kit (Takara, cat no- #6110A), the first strand of cDNA was synthesised according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Genomic titer was determined using a Lenti-X GoStix Plus kit (Takara). Lentivirus was functionally titrated previously using parallel preparation of GFP-expressing viral particles (Campeau et al. ...
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bioRxiv - Cancer Biology 2024Quote: ... ChIP libraries were prepared with the ThruPLEX® DNA-Seq Kit (Takara) and sequenced on the Illumina NovaSeq6000 SP-100 platform ...
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bioRxiv - Cell Biology 2024Quote: ... Libraries were constructed using a SMARTer Stranded Total RNA-Seq kit (Clontech). Directional mRNA-Seq of sorted cell populations was conducted using the Illumina HiSeq 6000 system ...
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bioRxiv - Microbiology 2024Quote: ... By using a PrimeScriptTM RT reagent Kit with gDNA eraser (Takara, Japan), reverse transcription-PCR was carried out on 1 μg of total RNA and primers are listed in Table EV2 ...
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bioRxiv - Microbiology 2024Quote: ... with the One Step PrimeScript™ RT-PCR Kit (Takara Bio, RR064A). The following primers targeting a 244-base amplicon of the IAV M segment were used:
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bioRxiv - Immunology 2024Quote: Reverse transcription was performed by using PrimeScript™ RT Reagent Kit (Takara). Quantitative PCR was run using SYBR green Master Mix or TaqMan Fast Advanced Master Mix on a QuantStudio 3 (Applied Biosystems ...
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bioRxiv - Molecular Biology 2021Quote: Slide embedded tissue sections were incubated in PBS containing 0.2 mg/ml saponin (Nacalai teques) and 10 μg/ml Proteinase K (Takara Bio) for 20 min at RT ...
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bioRxiv - Molecular Biology 2021Quote: TET-ON stable HEK293 cells with the Tetracycline-inducible expression constructs were grown in DMEM supplemented with 10% TET-approved FCS (Clontech) and induction of expression of respective gene product was carried out for indicated time durations using 400 ng/ml Doxycycline (SIGMA) ...
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bioRxiv - Cancer Biology 2019Quote: ... we performed 30 separate 100 μL PCR reactions with 10 μg genomic DNA in each reaction using ExTaq DNA Polymerase (Clontech) then combined the resultant amplicons ...
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bioRxiv - Biophysics 2021Quote: ... Cells were seeded at 25-30% confluency onto the prepared coverslips placed in 6-well plates in cell imaging media comprising phenol-red free DMEM (HyClone) supplemented with 10% (v/v) FBS (ClonTech) and 1 mM sodium pyruvate and allowed to settle for at least 4h ...
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bioRxiv - Microbiology 2020Quote: ... Reactions were performed in a total volume of 20 μl containing 10 μl of TB Green Fast qPCR Mix (#RR430A, TAKARA), 0.4 μl of ROX reference dye II ...
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bioRxiv - Bioengineering 2022Quote: ... were reverse transcribed into cDNA in a volume of 10 µl using the PrimeScript RT Master Mix (Perfect Real Time, TaKaRa) (37°C ...
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bioRxiv - Cell Biology 2022Quote: ... JCaM.1-derived cell lines with tetracycline-inducible gene expression system were maintained in RPMI 1640 supplemented with 10 % tetracycline-negative FBS (Clontech). Cells were routinely tested and found negative for mycoplasma and were not STR profiled.
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bioRxiv - Plant Biology 2021Quote: ... medium and 10-fold dilutions of these cultures were dropped on SD control (−2) and selective medium additionally lacking His (−3) (Clontech). Empty vectors were used as negative controls ...
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bioRxiv - Cancer Biology 2020Quote: ... Human melanoma cell lines and Hema-LP were overlaid with viral particles diluted in DMEM/1x Glutamax with 10% TET System Approved FBS (Clontech) supplemented with 5 μg/ml polybrene (Sigma-Aldrich) ...
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bioRxiv - Systems Biology 2020Quote: ... The cell lines were cultured in IMDM medium (Quality Biological) supplemented with 10% (v/v) Tet System Approved fetal calf serum (Takara Bio/previously Clontech), 100 U/mL penicillin ...
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bioRxiv - Genetics 2021Quote: ... The composition of the reaction mixture for the PCR was as follows: 10 μl Premix Taq (Ex Taq Version 2.0 plus dye, TaKaRa, Japan), 0.3 μl each of forward and reverse primer (10μM) ...
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bioRxiv - Pathology 2021Quote: ... 5 µL of 1:10 diluted cDNA samples was used as the qRT-PCR template with 0.5 µM gene-specific primers and 10 µL SYBR Premix Ex Taq II (Takara, China) in a total volume of 20 µL ...
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bioRxiv - Cell Biology 2021Quote: ... Expression from the CMV-TetO2 promoter in Flp-In T-REx cells was induced by treatment with 10 ng/mL doxycycline (Clontech) for 24 hours ...
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African Swine Fever Virus CD2v protein promotes β-Interferon expression and apoptosis in swine cellsbioRxiv - Microbiology 2020Quote: ... ORFV120 and ORFV113 coding sequences were PCR-amplified from orf virus strain OV-IA82 genome and cloned into p3xFlag-CMV-10 vector (pFlag) (Clontech).