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Citations for Takara Bio :
151 - 200 of 229 citations for Toxoplasma gondii IgG antigen RH strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... a DNA fragment of the TkR86C coding region was amplified from cDNA from the Canton-S wild type strain by PCR (PrimeSTAR GXL, TaKaRa Clontech) with primers that had NotI and XbaI sites at 5’ and 3’ ends ...
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bioRxiv - Pathology 2022Quote: ... The bait and prey vectors were co-transformed into yeast strain Y2H Gold according to the Yeast Protocols Handbook (Clontech), diluted on SD/-Leu-Trp/AbA for selection of positive colonies ...
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bioRxiv - Microbiology 2022Quote: ... Each of these prey plasmids and previously constructed bait plasmids were co-transformed into Y2HGold yeast strain using Yeastmaker Yeast Transformation System 2 (TaKaRa). The transformation mix was spread on DDO/X and QDO/X/A agar plates and incubated at 30°C for 3-5 days ...
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bioRxiv - Microbiology 2022Quote: ... The purified ds cDNA and SmaI linearized pGADT7-Rec were co-transformed into Y187 yeast strain using Yeastmaker Yeast Transformation System 2 (TaKaRa). The transformation mix was spread on to the SD/-Leu agar plates and incubated at 30°C for 3–4 days ...
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bioRxiv - Plant Biology 2022Quote: ... PtoATPE and PtoPSBB) were co-transformed into the Y1HGold yeast strain using the Matchmaker One-Hybrid Library Construction and Screening Kit (Clontech), respectively ...
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bioRxiv - Plant Biology 2021Quote: ... All combinations were transferred to the SD/-Leu-Trp medium by the yeast strain Y2H (Clontech, Mountain View, CA, USA), and then transferred to the SD/-Leu-Trp medium ...
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bioRxiv - Plant Biology 2020Quote: The transformation of each plasmid for the respective domain configurations was performed as described by Gietz and Schiestl (2007) using Saccharomyces cerevisiae strain HF7C (Clontech).
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bioRxiv - Pathology 2019Quote: ... two independent experiments were performed by mating the aliquoted cells with the Y2HGold strain harboring the bait construct pGBKT7-FgANK1 following instruction of the Matchmaker Gold yeast two-hybrid system (Clontech). Interaction clones were selected on 50 SD/-Trp/-Leu/X-α-Gal/AbA (40 μg/ml X-α-Gal and 200 ng/ml Aureobasidin A ...
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African Swine Fever Virus CD2v protein promotes β-Interferon expression and apoptosis in swine cellsbioRxiv - Microbiology 2020Quote: ... ORFV120 and ORFV113 coding sequences were PCR-amplified from orf virus strain OV-IA82 genome and cloned into p3xFlag-CMV-10 vector (pFlag) (Clontech).
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bioRxiv - Plant Biology 2020Quote: ... bait plasmids and prey plasmids were co-transformed into yeast strain AH109 according to the manufacturer’s protocol of GAL4-based yeast two-hybrid system (Clontech, USA). Then ...
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant OmpA-pGBKT7 and Flg-pGBKT7 were finally transformed into Y2H Gold yeast strain separately using Yeastmaker yeast transformation system (Takara, Clontech).
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bioRxiv - Microbiology 2022Quote: ... 10 ng of linearized pDEST32 empty vector was co-transformed with 3 μl of PCR product to achieve recombinational cloning by gap-repair in Y2H Gold yeast strain (Clontech) expressing AD-fused CP204L (pPC86 vector) ...
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bioRxiv - Cell Biology 2022Quote: ... The yeast strain AH109 was transformed with the constructs in destination vectors using lithium acetate transformation (Yeast Protocols Handbook PT3024-1, Clontech). The transformants were grown on synthetic defined medium (0.67% yeast nitrogen base ...
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bioRxiv - Molecular Biology 2022Quote: ... The freshly extracted plasmids were transformed into yeast strain Y2H gold by using a yeast transformation kit (TaKaRa, Beijing, China) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... The recombinant plasmid of pGBKT7-GhERF105a/d and plasmid with empty vector were transformed respectively into strain AH109 yeast cells according to the manufacturer’s instructions (Clontech, USA). The transactivation activity was determined by the growth on SD/-Trp and SD/-Trp/-X-a-gal (5-bromo-4-chloro-3-indolyl-α-D-galactopyranoside ...
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bioRxiv - Plant Biology 2023Quote: ... The heat treatment lasted for 10 min for S. cerevisiae strains PJ69-4A (James et al. 1996) and AH109 (Clontech), used for the classical Y2H assays ...
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bioRxiv - Plant Biology 2023Quote: ... pYES2-SiHDA9 and empty pYES2 vector (EV) were transformed individually into Saccharomyces cerevisiae strain W303 using Yeastmaker Yeast Transformation System (Clontech). The transformed cells were screened on SD/-ura medium with 2% (w/v ...
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bioRxiv - Immunology 2022Quote: ... Mate & Plate™ cDNA Library in a pGADT7 vector with the GAL4 DNA-activation domain (AD) and pre-transformed into yeast strain Y187 was acquired from Takara Bio ...
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bioRxiv - Plant Biology 2023Quote: ... the strain carrying the pLBD16::AbAi bait was tested for autoactivation by using empty prey strains carrying empty vector of pDEST-22 under various concentrations of Aureobasidin A (AbA) (Takara) (0 ...
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bioRxiv - Plant Biology 2023Quote: ... The LexA fusion constructs (bait) and the activation domain fusion constructs (prey) were co-transformed into yeast strain EGY48[p8op-lacZ] (CLONTECH). Clones containing both constructs were selected on medium lacking His ...
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bioRxiv - Biochemistry 2023Quote: ... Constructs used to test protein-protein interactions were co-transformed into the yeast Saccharomyces cerevisiae strain AH109 using the Matchmaker Gold yeast two-hybrid system (Clontech). Empty pDEST-GBKT7 and pDEST-GADKT7 vectors were used as negative control ...
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bioRxiv - Plant Biology 2023Quote: ... Direct protein-protein interaction was confirmed by co-transformation of the respective plasmids into the yeast strain AH109 using the Matchmaker GAL4 Two-hybrid System (Clontech), followed by a selection of transformants on 2% SD (-Leu/-Trp ...
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bioRxiv - Plant Biology 2024Quote: The HIS3 and ADE2 reporter genes were employed to assess the protein interactions in the yeast strain Y2HGold (Takara Bio). Yeast transformation was carried out using the lithium acetate (LiAc)-mediated method ...
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bioRxiv - Plant Biology 2024Quote: ... Bait and prey constructs were transformed into the haploid yeast strains Y2HGold and Y187 (Matchmaker Gold Yeast Two-Hybrid System, Clontech), respectively ...
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bioRxiv - Cell Biology 2024Quote: ... Pairs of pGBKT7 and pGADT7 plasmid were co-transformed into the yeast strain AH109 following the MatchmakerTM GAL4 Two-Hybrid System instructions (Clontech). Primary transformants were selected on synthetic drop-out (SD ...
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bioRxiv - Plant Biology 2023Quote: ... the Y2HGold strain was transformed with the bait vector (pGBKT7-SR45) and then mated with strain Y187 containing an Arabidopsis cDNA library (Mate and Plate Library-Universal Arabidopsis, Clontech) (Stankovic et al. ...
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bioRxiv - Molecular Biology 2024Quote: - IgG detector solution V2 (1:2000 dilution, Takara, Cat# T7122A-1).
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bioRxiv - Molecular Biology 2024Quote: IgG detector solution V2 (1:2000 dilution, Takara, Cat# T7122A-1).
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bioRxiv - Biochemistry 2020Quote: ... falciparum 3D7 strain and homology regions were PCR amplified using PrimeStar GXL DNA polymerase (Takara Bio Inc., Mountain View, CA, USA). The 3’-untranslated homology region (3’-UTR ...
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bioRxiv - Plant Biology 2022Quote: ... The recombinant constructs was transformed into Y1H gold strain and Y1H assay was performed using Matchmaker® Gold Yeast One-hybrid screening system (Takara) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant OmpA-pGBKT7 and Flg-pGBKT7 were finally transformed into Y2H Gold yeast strain separately using Yeastmaker yeast transformation system (Takara, Clontech).
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bioRxiv - Microbiology 2019Quote: ... discoideum were amplified from cDNA (Robinson and Spudich 2000) or genomic DNA of the Ax2 strain using PrimeStar Max DNA polymerase (Takara Bio) and the designed primers ...
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bioRxiv - Neuroscience 2021Quote: ... a DNA fragment of the TkR86C coding region was amplified from cDNA from the Canton-S wild type strain by PCR (PrimeSTAR GXL, TaKaRa Clontech) with primers that had NotI and XbaI sites at 5’ and 3’ ends ...
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bioRxiv - Plant Biology 2019Quote: ... pGBKT7-Pha21 and pGADT7-UBQ was co-transformed into AH109 yeast strain for yeast two-hybrid assay by using the Make Your Own “Mate & Plate” Library System (Takara Bio) following the user manual ...
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bioRxiv - Molecular Biology 2020Quote: Yeast-2-hybrid assays were performed with the plasmids and strains from the Matchmaker Gold Yeast Two-Hybrid System (Takara Bio) according to manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... StNPR1 or StNPR3/4 cds) construct combinations were transformed into the Y2H Gold strain using the Matchmaker Gold Yeast Two-Hybrid System (Clontech, USA) and the transformants selected on control SD media without Leu and Trp (-L-W) ...
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bioRxiv - Plant Biology 2023Quote: OsbZIP1.1 and OsbZIP1.2 CDS were cloned in pDEST-GBKTT7 (CD3-764) obtained from TAIR (https://www.arabidopsis. org) and transformed in the AH109 yeast strain (Clontech, TaKaRa Bio, USA). Yeast transformation was performed as described in the Yeastmaker Yeast Transformation System 2 User Manual (Clontech ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant constructs were introduced into Y1H gold strain and Y1H assay was performed using Matchmaker® Gold Yeast One-hybrid screening system (Takara) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: The recombinant pGBKT7-PGRP3 bait construct was transformed into yeast strain Y2HGold via the lithium acetate method per manufacturer protocol (Takara Bio). Transformants were cultured on SD/-Trp (SDO ...
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bioRxiv - Biochemistry 2023Quote: ... coli BW25113 WT strain by PCR and inserted in pZA23 plasmid using the In-Fusion® HD Cloning Plus CE Kit (Takara).
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bioRxiv - Plant Biology 2023Quote: ... the correct full-length sequences were determined for the laboratory strains using a SMARTer RACE 5’/3’ kit (Takara, Shiga, Japan). These sequences were aligned by ClustalW ...
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bioRxiv - Plant Biology 2023Quote: ... were used to co-transform yeast strain AH109 and colonies carrying both vectors were selected on SD medium without tryptophan (-W) and leucine (-L) (TaKaRa 630317) at 28°C ...
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bioRxiv - Molecular Biology 2024Quote: ... Total RNA from two replicates of each strain were extracted at about 20 generations after refeeding using RNAiso Plus (TaKaRa, 9108).
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bioRxiv - Cell Biology 2020Quote: The yeast strain AH109 was co-transfected with bait vector full-length human SNX4 or Lamin cloned into pGBKT7 (Clontech, Oxford, UK) against a pray library of FL SNX-BARs ...
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bioRxiv - Plant Biology 2021Quote: ... SlGA2ox6 and SlGID1b-1 were constructed into pAbAi plasmid and transformed into Y1HGold yeast strain under the guidance of Yeastmaker™ Yeast Transformation System 2 (TAKARA, Japan). The recombinant yeast strain was confirmed by PCR conducted with Matchmaker™ Insert Check PCR Mix (TAKARA ...
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bioRxiv - Plant Biology 2020Quote: ... DBD) were transformed into Y2H Gold and prey constructs (GAL4 activation domain, AD) into Y187 strains by LiAc method (Takara Bio USA). Haploid strains were mated to produce diploid strains to test for interactions ...
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bioRxiv - Plant Biology 2021Quote: ... The yeast strain AH109 was used for the yeast two-hybrid assay according to MatchmakerTM Two-Hybrid System 3 (Clontech, Palo Alto) and grown on YPD (full medium ...
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bioRxiv - Plant Biology 2020Quote: ... while specific AD and BD recombinant plasmid pairs were used to co-transform yeast strain AH109 cells according to the Yeast Protocols Handbook (Takara, Dalian, China).
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bioRxiv - Cell Biology 2022Quote: ... positive control (pGBKT7-53) and negative control bait plasmids (pGBKT7-Lam) were transformed into yeast strain Y2HGold (Takara Bio, Mountain View, USA) while pGADT7-CmVPS41s and pGADT7-T control prey plasmids were transformed into yeast strain Y187 following the Yeastmaker Yeast Transformation System 2 instructions (Clontech by Takara Bio ...
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bioRxiv - Plant Biology 2023Quote: OsbZIP1.1 and OsbZIP1.2 CDS were cloned in pDEST-GBKTT7 (CD3-764) obtained from TAIR (https://www.arabidopsis. org) and transformed in the AH109 yeast strain (Clontech, TaKaRa Bio, USA). Yeast transformation was performed as described in the Yeastmaker Yeast Transformation System 2 User Manual (Clontech ...