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Citations for Takara Bio :
151 - 200 of 616 citations for SARS Coronavirus Membrane Protein Matrix E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2022Quote: ... coli BL21(DE3) carrying the pGro7 plasmid (Takara, Shiga, Japan) for GroEL/ES chaperones co-expression48 ...
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bioRxiv - Molecular Biology 2024Quote: ... and then transformed in Escherichia coli HST08 competent cells (Takara). The sequence integrity of inserts carried by transformants were verified by Sanger sequencing ...
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bioRxiv - Biophysics 2022Quote: ... E-cadherin (24E10-Cell Signaling Technology; DECMA1-Sigma Aldrich, ECCD2, Takara Bio) (1:100)and paxillin (Abcam ...
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bioRxiv - Microbiology 2020Quote: ... SARS-CoV-2 RBDs were purified using 1 or 5 ml HisTALON superflow cartridges (Takara Bio) and subsequently buffer exchanged into Cytiva 1x HBS- N buffer or PBS.
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2 Beta RBD was purified using a 5 mL HisTalon Superflow cartridge (Takara Bio) followed by buffer exchange into PBS using a HiPrep 26/10 desalting column (Cytiva).
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bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 RBDs were purified using 1 or 5 mL HisTALON superflow cartridges (Takara Bio) and subsequently buffer exchanged into 1x HBS-N buffer (Cytiva ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 RBDs were purified using a Cobalt affinity column (HisTALON Superflow column from Takara or HiTrap TALON crude column from Cytiva ...
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bioRxiv - Microbiology 2022Quote: ... CydDC was purified via His6-tag affinity chromatography using a Talon (Co-IMAC) affinity matrix (Takara Bio Inc.). For column conditioning and washing step I (10 column volumes [CV] ...
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bioRxiv - Neuroscience 2021Quote: ... coli BL21 and purified on 5 mL Talon column (Clontech®) loaded with Cobalt ...
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bioRxiv - Synthetic Biology 2020Quote: ... coli Rosetta (DE3) (DAD2 constructs) or BL21 (DE3) containing pGro7 (Takara) (ShHTL7 constructs ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... coli Stellar™ Competent Cells (TAKARA Bio Inc., San Jose, CA) was added to pre-chilled centrifuge tubes and chilled on ice for 10 minutes ...
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bioRxiv - Microbiology 2023Quote: ... Escherichia coli strains StellarTM (Takara bio, San Jose, CA, United States) were grown at 37°C in LB broth (Difco ...
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bioRxiv - Immunology 2020Quote: Levels of p24 antigen in the purified SARS-CoV-2 pseudotype solution was measured by ELISA (Takara). Mouse sera were heat inactivated ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... SARS-COV-2 virus detection was performed using the One Step PrimeScript RT-PCR kit (TaKaRa, Japan) on the LightCycler 480 Real-Time PCR system (Roche ...
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bioRxiv - Immunology 2021Quote: ... wild-type SARS-CoV-2 RBD was purified using a 5 mL HisTALON superflow cartridge (Takara Bio) followed by size exclusion chromatography using a Superdex 200 10/300 GL column pre-equilibrated in 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: ... S5C-E was generated by fusing human Rac1 cDNA into EGFP-C1 (Clontech) and kindly provided by Dr ...
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bioRxiv - Microbiology 2023Quote: ... RNase E was then purified using TALON® Metal Affinity Resin (Takara Bio). Protein purity was confirmed by SDS-polyacrylamide gel electrophoresis (PAGE ...
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bioRxiv - Microbiology 2020Quote: ... The ligated plasmid was then transformed to Escherichia coli HST08 (Takara Bio) and selected on chloramphenicol plates ...
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bioRxiv - Physiology 2020Quote: ... coli Stellar cells (Clontech/Takara Bio USA Inc., Mountain View, CA, USA). Cells were grown to an OD600 of about 0.5 and afterwards harvested ...
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bioRxiv - Microbiology 2021Quote: ... Genetic manipulations were carried out in Escherichia coli StellarTM competent cells (Clontech). Primers and plasmids used in this study are listed in Table S2 and S3 ...
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bioRxiv - Molecular Biology 2022Quote: ... coli JM109 cell pellets by using NucleoBond Xtra Midi (TaKaRa Bio Inc.) and protocol for low copy plasmids ...
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Map7D2 and Map7D1 facilitate microtubule stabilization through distinct mechanisms in neuronal cellsbioRxiv - Cell Biology 2022Quote: An RNA blot membrane (Clontech) was hybridized with the 32P-labeled full coding sequence of rMap7D2 ...
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bioRxiv - Cell Biology 2023Quote: ... E-cadherin antibody (HECD-1, 1:1000 IF, 1:1000 WB) was from Takara Bio ...
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bioRxiv - Cell Biology 2023Quote: ... TagRFP-CENP-E 2111-C were cloned into the pLVX-IRES-Puro vector (Clontech) by PCR-based Gibson assembly method.
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bioRxiv - Evolutionary Biology 2020Quote: ... followed by transfection into Escherichia coli DH5α competent cells (Takara Bio, Shiga, Japan). Positive clones were identified by blue-white screening and at least three positive clones were sequenced ...
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bioRxiv - Biochemistry 2022Quote: ... coli and cloned into pET28a using In-Fusion cloning (Takara Bio Europe, France).
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bioRxiv - Microbiology 2022Quote: The plasmids of the SARS-CoV-2 cDNA library as cloned in the pLVX-EF1alpha-IRES-Puro (Takara/Clontech) vector were a kind gift from Prof ...
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bioRxiv - Microbiology 2022Quote: The plasmids of the SARS-CoV-2 cDNA library as cloned in the pLVX-EF1alpha-IRES-Puro (Takara/Clontech) vector were a kind gift from Prof ...
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bioRxiv - Microbiology 2023Quote: ... or Omicron variant (G339D) using a SARS- CoV-2 Direct Detection RT-qPCR Kit (TaKaRa Bio Inc., Shiga, Japan) with each specific primer/probe ...
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bioRxiv - Microbiology 2023Quote: ... the nine pmW118 plasmid vectors were subjected to amplification of the cDNA fragments (F1-F9-10) of SARS-CoV-2 XBB.1 and XBB.1.5 by PrimeSTAR GXL DNA polymerase (Takara) with the primer sets24 ...
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bioRxiv - Cell Biology 2023Quote: HaCaT cells were treated with the E-cadherin-blocking antibody (SHE78-7, Takara, Shiga, Japan), PY-60 (Axon Medchem ...
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bioRxiv - Microbiology 2019Quote: ... The resultant plasmid was cloned into Escherichia coli JM109 competent cells (TAKARA, Shiga, Japan) and transformed into S ...
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bioRxiv - Microbiology 2019Quote: ... The constructed plasmid was cloned into Escherichia coli JM109 competent cells (TAKARA, Shiga, Japan) and pre-introduced into restriction-deficient S ...
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bioRxiv - Cell Biology 2022Quote: ... coli DH5⊡ competent cells and isolated using a midi-prep kit (Takara Bio, 740420.10), and validated by gel electrophoresis.
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bioRxiv - Biochemistry 2023Quote: The plasmid library was transformed into Escherichia coli strain BL21(DE3)-pGro7/GroEL (TaKaRa). Cells were grown overnight at 37°C on nitrocellulose membranes (Amersham™ Protran™ ...
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bioRxiv - Microbiology 2023Quote: ... These recombinant plasmids were transformed into Escherichia coli BL21 (Takara Bio Co Ltd, Japan) and the transformed cells were cultured at 37°C until the OD600 reached at 0.6–0.8 ...
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bioRxiv - Systems Biology 2023Quote: ... coli using the Nucleobond Xtra Midi prep kit (Takara Bio, San Jose, California, USA) following the manufacturer’s protocol (Cat# 740422.50) ...
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bioRxiv - Microbiology 2022Quote: ... The viral RNA copy number was standardized with a SARS-CoV-2 direct detection RT-qPCR kit (Takara, Cat# RC300A). Fluorescent signals were acquired using QuantStudio 3 Real-Time PCR system (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... covering the full-length SARS-CoV- 2 genome were amplified by PCR using a PrimeSTAR GXL DNA polymerase (TaKaRa Bio), the synthesized cDNA and specific primer sets from CoV-2-G1-Fw to CoV-2-G10-Rv designed previously (21) ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 RNA quantification was performed by RT-qPCR targeting the S gene of SARS-CoV-2 using One Step PrimeScript RT-PCR Kit (Takara) with the following SARS-CoV-2 specific primers and probes ...
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bioRxiv - Microbiology 2022Quote: ... Ct values were obtained from PCR tests conducted as administrative tests at the Toyama Institute of Health using the SARS-CoV-2 direct detection RT-qPCR test kit from Takara Bio Inc ...
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bioRxiv - Microbiology 2022Quote: ... The viral RNA copy number was standardized with a SARS-CoV-2 direct detection RT-qPCR kit (Takara, Cat# RC300A). Fluorescent signals were acquired using QuantStudio Real-Time PCR system (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... The viral RNA copy number was standardized with a SARS-CoV-2 direct detection RT-qPCR kit (Takara, Cat# RC300A). Fluorescent signals were acquired using a QuantStudio 1 Real-Time PCR system (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... The viral RNA copy number was standardized with a SARS-CoV-2 direct detection RT-qPCR kit (Takara, Cat# RC300A). Fluorescent signals were acquired using a QuantStudio 1 Real-Time PCR system (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... The nine fragments of SARS-CoV-2 and the UTR linker for SARS-CoV-2 were prepared by PCR using PrimeSTAR GXL DNA polymerase (Takara). After gel purification of the fragments ...
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bioRxiv - Microbiology 2023Quote: ... The viral RNA copy number was standardized with a SARS-CoV-2 direct detection RT-qPCR kit (Takara, Cat# RC300A). Fluorescent signals were acquired using a QuantStudio 1 Real-Time PCR system (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... The viral RNA copy number was standardized using a SARS-CoV-2 direct detection RT-qPCR kit (Takara, Cat# RC300A). Fluorescent signals from resulting PCR products were acquired using a Thermal Cycler Dice Real Time System III (Takara).
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bioRxiv - Microbiology 2023Quote: ... The viral RNA copy number was standardized with a SARS-CoV-2 direct detection RT-qPCR kit (Takara, Cat# RC300A). Fluorescent signals were acquired using a QuantStudio 1 Real-Time PCR system (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2024Quote: ... The samples were stained with anti-mouse E-cadherin monoclonal antibody ECCD-2 (1:100, Takara) for 16 h at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... SARS-CoV2 Mpro and C300S Mpro were purified first by affinity chromatography using TALON™ cobalt-based affinity Resin (Takara Bio). The His6-tag was cleaved off by PreScission protease and the resulting authentic 306 amino acid Mpro (see Figure S1A in supplemental material ...