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Citations for Takara Bio :
151 - 200 of 5045 citations for Retinol Binding Protein High Sensitivity ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The recombinant Cas9 protein (TAKARA) and the sgRNA were incubated for 10 min at 37°C ...
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bioRxiv - Plant Biology 2022Quote: ... bait proteins were cloned into the pGBKT7 vector which encodes the GAL4 DNA binding domain and then transformed into the Y2H Gold strain (Clontech, Mountain View, California) and plated on SD/- Trp to select for positive transformants ...
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bioRxiv - Microbiology 2020Quote: ... Ad5 stocks were tittered by detection of the Ad hexon protein using Adeno-X™ Rapid Titer Kit (Cat. No. 632250, Takara, Clontech). The Ad5 CPE was dose-dependent ...
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bioRxiv - Biochemistry 2023Quote: ... Expression plasmids for other His-mCherry or His-GFP fusion proteins were constructed using in-Fusion HD Cloning Kit (Takara Bio, Inc.). All mCherry/GFP fusion proteins were expressed in E ...
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bioRxiv - Molecular Biology 2021Quote: Secretion in PIP in NHLFs treated with MRG-201 or MRG-229 was conducted by collecting the supernatant of treated cells and performing procollagen type I PIP ELISA (Takara).
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bioRxiv - Biophysics 2019Quote: ... cDNAs encoding cyan fluorescent protein (CFP) and yellow fluorescent protein (YFP) were purchased from Clontech Com ...
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bioRxiv - Microbiology 2020Quote: ... The PCRs were performed with a high-fidelity DNA polymerase (PrimeStarMax, Takara) (see section Detailed protocol for chimeric PCR for mutation in A ...
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bioRxiv - Synthetic Biology 2021Quote: PCR was performed using the high-fidelity polymerase PrimeSTAR GXL DNA (Clontech). Golden Gate assembly was executed using the NEB Golden Gate Assembly Kit ...
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bioRxiv - Microbiology 2021Quote: ... The PCRs were performed with a high-fidelity DNA polymerase (PrimeStarMax, Takara).
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bioRxiv - Plant Biology 2023Quote: ... Phusion High-Fidelity DNA Polymerase (Thermo) or CloneAmp HiFi PCR Premix (Takara) was used to PCR amplify all the above sequences with primers listed in Table S2 ...
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bioRxiv - Microbiology 2024Quote: ... The amplification was performed using high-fidelity PrimeSTAR GXL DNA polymerase (Takara). Next ...
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bioRxiv - Neuroscience 2022Quote: ... Constructs for human Tara were prepared by cloning full-length TRIOBP1 (Trio and F-actin binding protein1) isoform into pEGFP-C3 (Clontech, Mountain View, CA, USA), pFLAG-CMV2 (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2022Quote: Protein-protein interactions were assayed with the Matchmaker yeast two-hybrid system (Clontech, Mountain View, CA). ORFs of AoHse was amplified from first-strand cDNA of A ...
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bioRxiv - Neuroscience 2020Quote: Enhanced green fluorescent protein (EGFP, Clontech), Tag-blue fluorescent protein (Tag-BFP ...
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bioRxiv - Molecular Biology 2022Quote: ... protein was estimated using BCA (TaKaRa), and 100 µg protein was incubated with 20 µl packed protein G-sepharose beads (Sigma P3296 ...
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bioRxiv - Neuroscience 2023Quote: ... 5) cDNA coding eGFP protein (Clontech). 6 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The α1-subunit genes were inserted at the PH promoter of vectors already containing the corresponding β1-subunit proteins using In-Fusion® HD Cloning Kit (Takara Bio, USA Inc.) and control sequenced ...
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bioRxiv - Biochemistry 2023Quote: ... The PCR fragments of spike protein DNA were cloned into linearized pMRNAXP vector using In-Fusion® HD Cloning Kit (Clontech® Laboratories, Inc.). The cloning mixtures were transformed to One Shot™ TOP10 Chemically Competent E ...
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bioRxiv - Biochemistry 2021Quote: ... an aliquot of the lysate was used to measure protein concentration by BCA protein assay (Takara Bio). The lysate was mixed with Optiphase Hisafe 3 (PerkinElmer) ...
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bioRxiv - Plant Biology 2020Quote: ... The cDNA was amplified using high-fidelity PrimeSTAR® Max DNA Polymerase (TaKaRa) followed by PCR clean-up ...
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bioRxiv - Microbiology 2021Quote: ... Allele fragments were generated using the high-fidelity PrimeSTAR Max polymerase (Takara Bio), assembled into strain-specific pEXG2 plasmids in E ...
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bioRxiv - Cell Biology 2021Quote: ... as two overlapping fragments using high-fidelity Takara LA Taq (Takara, Cat # RR002M). Primer details can be found in Supplementary Table 2 ...
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bioRxiv - Systems Biology 2021Quote: ... all PCRs utilized a high fidelity polymerase (CloneAmp HiFi PCR Premix, Takara 639298). Plasmids contained components of the synthetic inducible system flanked by homology to the intended genomic integration locus and were restricted prior to transformation ...
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bioRxiv - Genetics 2022Quote: ... Extraction of high-molecular-weight DNAs was performed using NucleoBond HMW DNA (TAKARA). Library preparation was performed using the Ligation Sequencing Kit (Oxford Nanopore Technologies ...
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bioRxiv - Plant Biology 2022Quote: ... purified with a high-salt solution for precipitation (Takara Bio Inc., Ohtsu, Japan) and reverse transcribed with ReverTra Ace (Toyobo) ...
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bioRxiv - Plant Biology 2021Quote: Protein-protein interactions via yeast-two-hybrid (Y2H) assays were performed as described in the Matchmaker protocol (Clontech). HvRACB (amino acids 1-193 ...
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bioRxiv - Biochemistry 2023Quote: ... Uncleaved protein and cleaved His6-Ztag domains were separated from cleaved protein by incubation with TALON resin (Clontech). The supernatant was buffer exchanged to MES A buffer (20 mM MES ...
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bioRxiv - Microbiology 2020Quote: Tissue protein lysates were purchased from Takara. Details for each lysate are shown in Table S1 ...
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bioRxiv - Neuroscience 2020Quote: ... cell-free protein expression system from Takara Bio Inc ...
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bioRxiv - Biochemistry 2021Quote: ... Protein was purified using Talon resin (Clontech), eluted in 200 mM imidazole ...
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bioRxiv - Zoology 2020Quote: ... The protein concentration was measured by TaKaRa BCA Protein Assay Kit (Takara Bio ...
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bioRxiv - Molecular Biology 2019Quote: ... and protein was incubated with Talon (Clontech), 0.5 ml resin per litre cell culture for 1 h at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... the Capturem Protein A technology from Takara was employed according to the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... subtilis Secretory Protein Expression System (TAKARA Bio). For signal peptide library transformation into B ...
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bioRxiv - Genetics 2019Quote: For high fidelity PCR reactions we followed manufacturer’s instructions using either Extaq (TaKaRa # RR001C), KOD hotstart (Merck Millipore # 71086 ...
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bioRxiv - Zoology 2021Quote: ... High-fidelity Takara Ex Taq PCR reagents were employed (Takara Bio Inc., Otsu, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Zoology 2019Quote: ... High-fidelity Takara Ex Taq PCR reagents were employed (Takara Bio Inc., Otsu, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... Primer Star high fidelity DNA polymerase and restriction enzymes were purchased from TaKaRa (Beijing). Ordinary Taq enzyme was purchased from Tiangen (Beijing).
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bioRxiv - Cancer Biology 2024Quote: High-quality RNA was isolated from cells or tissues using Trizol reagent (AJF1807A, Takara) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: Expression plasmids for fusion proteins of GFP and tardigrade proteins were constructed by Gibson assembly into pEGFP-N1 (Clontech) of the tardigrade cDNA (obtained by gene synthesis from Integrated DNA ...
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bioRxiv - Molecular Biology 2020Quote: A codon-optimized synthetic gene corresponding to the full length nucleocapsid protein (Thermo GeneArt, Regensburg, Germany) was cloned into a modified pET28a vector using the In-Fusion cloning kit (Takara Bio Saint-Germain-en-Laye, France). The resulting plasmid encoded for an N-terminal His6 tag followed by thrombin and tobacco etch virus cleavage sequences upstream of the full-length nucleocapsid protein sequence ...
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bioRxiv - Cell Biology 2019Quote: ... and both proteins were separated from each other and from a protein encoded by blasticidin resistance gene (cloned from pQCXIB #631516, Clontech) by self-cleavage peptide P2A ...
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Osh6 requires Ist2 for localization to the ER-PM contacts and efficient phosphatidylserine transportbioRxiv - Cell Biology 2020Quote: For testing protein-protein interactions Matchmaker™ GAL4 Two-Hybrid System 3 was used according to the manufacturer’s instructions (Clontech). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: ... Eukaryotic expression vectors encoding green fluorescent protein (GFP)-tagged proteins were generated by inserting PCR-amplified fragments into pd1-EGFP-N1 vector (6073-1, Clontech). Prokaryotic plasmids encoding GST-fusion proteins were constructed using pGEX-4T-1 bacterial expression vector (27-4580-01 ...
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bioRxiv - Cancer Biology 2021Quote: High-throughput quantitative PCR (qPCR) was performed using the SmartChip system (Takara Bio, Kusatsu, JAPAN) on 47 oral melanoma samples to detect focal amplifications on tumor DNA on CFA 10 and CFA 30 ...
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bioRxiv - Synthetic Biology 2020Quote: ... including the promoter and the full CDS was amplified by high-fidelity PCR (TaKaRa Bio) and sequenced on an Illumina MiSeq system with transposase based library preparation kit (Nextera XT ...
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bioRxiv - Genetics 2020Quote: ... used as the template for PCR amplification with high fidelity polymerase (RR006Q, Takara, Tokyo, Japan). Alternatively ...
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bioRxiv - Microbiology 2022Quote: ... The resulting plasmid was transfected into 293T cells using Lentiviral High Titer Packaging Mix (Takara) to obtain the lentiviral vector for TSG101–venus transduction ...
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bioRxiv - Evolutionary Biology 2022Quote: ... A 2nd PCR was performed with high-fidelity PrimeSTAR GXL DNA Polymerase (Takara Cat: R050B) to add the Nextera indices (Nextera XT primers Set A 96 Indexes ...
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bioRxiv - Bioengineering 2023Quote: ... DNA fragments were amplified with the high-fidelity polymerase PrimeSTAR Max DNA Polymerase from TaKaRa and colony PCR reactions were performed using DreamTaq polymerase (Thermo Fisher Scientific) ...