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Citations for Takara Bio :
151 - 200 of 4813 citations for QuantiFluo Acid Phosphatase Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Gal4-VP16-human SREBP-1c plasmid was prepared by insertion of a VP16-transactivation domain fused to a human SREBP-1c fragment from the 431st amino acid to the C-terminus (amino acids 431-1123) downstream of the Gal4-DNA binding domain sequence in pM vector (Clontech). The Gal4-RE-Luc plasmid and luciferase plasmid including sterol response element (SRE-Luc ...
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bioRxiv - Cell Biology 2021Quote: ... and LIM 3 (amino acids 361-421) were cloned into the multiple cloning site of pEGFP-N3 or pEGFP-C3 (Clontech) as previously described (Sala et al. ...
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bioRxiv - Cell Biology 2021Quote: ... Borr open reading frame corresponding to amino acids 113-221 was first amplified with a stop codon from LD36125 by PCR using PrimeStar (Takara) and cloned between AscI and NotI sites of pENTR (ThermoFisher ...
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bioRxiv - Immunology 2020Quote: ... For binding studies the 6xHis tag were removed from some Fabs by treatment with PreScission protease (MolBioTech; ThermoScientific) and the protein repurified on cobalt-nitrilotriacetic acid (Co-NTA) agarose (Clontech) followed by gel filtration chromatography on Superdex 200 (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... CPE 451 mCherry constructed by Gibson assembly by sub-cloning the 451 amino acids of CPE (without the Amphipathic Helix) into pmCherry-N1 (Clontech) vector using XhoI and BamHI restriction sites.As control vectors we used pEGFP-N3 and pmCherry-N1.All constructs were sequenced to confirm the fidelity of the process ...
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bioRxiv - Neuroscience 2023Quote: ... inserting an in-frame 15bp flexible linker sequence (encoding the amino acids GGGGA) followed by EGFP at the C-terminus using Infusion cloning (Clontech). For the Tg(NBT:ap2s1-gfp ...
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Molecular basis of proteolytic cleavage regulation by the extracellular matrix receptor dystroglycanbioRxiv - Biochemistry 2024Quote: ... A Flag tag x3 was inserted between amino acids 489 and 490 and a HA tag was inserted between amino acids 724 and 725 using In-Fusion Cloning Mix (Takara).
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bioRxiv - Plant Biology 2020Quote: Yeast two hybrid assay was performed using Matchmaker Gold Yeast Two-Hybrid System (Clontech). The OsSalT gene was cloned in-frame into the EcoRI and BamHI RE sites of the yeast vector ...
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bioRxiv - Plant Biology 2021Quote: ChIP-Seq assays were performed on whole seedlings using anti-GFP antibody (Clontech 632592). Seedlings at 14 DAS (5 g ...
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bioRxiv - Plant Biology 2021Quote: ... Liquid β-galactosidase assays were performed as described in the Yeast Protocols Handbook (Clontech). A Y2HGold yeast strain containing a bait vector was mated with the Y187 strain containing the pGADT7 vector and selected on SD/-Trp/-Leu media ...
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bioRxiv - Microbiology 2022Quote: ... The RT-qPCR assay was performed using the TB Green Fast qPCR Mix (Takara). The ribosomal protein L21 gene (RPL21 ...
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bioRxiv - Cell Biology 2019Quote: Total protein was extracted from cells by lysing with radioimmunoprecipitation assay buffer (Takara, Japan). Ten micrograms of protein were run on 4–20% gradient polyacrylamide gels (Thermo Fisher ...
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bioRxiv - Plant Biology 2022Quote: Yeast one-hybrid assays were performed using the Matchmaker Yeast One-Hybrid System (Clontech). The DNA fragment 1,000 bp upstream of the start codon ATG of DDRM2 was cloned into pHis2.1 vector and SOG1 was cloned into the pGADT7 vector ...
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bioRxiv - Microbiology 2023Quote: RNA obtained from the RIP assay sample was purified using NucleoSpin RNA Plus (TaKaRa), followed by library construction and RNA-seq analysis ...
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bioRxiv - Plant Biology 2023Quote: Y2H assays were performed with the MatchMaker GAL4 Two-Hybrid System 3 (Takara Bio). Saccharomyces cerevisiae strain AH109 was co-transformed with different pairs of pGADT7 and pGBKT7 harboring IREH1 or B1-Rafs ...
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bioRxiv - Plant Biology 2023Quote: ... the Y1H assays were performed following the manufacturer’s instruction (Clontech Laboratories, Inc., Kusatsu, Japan). The promoter sequence with and without mutation in ZDRE motif were artificially synthesized and cloned into the Kpn I-linearized pAbAi vector (Clontech Laboratories ...
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bioRxiv - Immunology 2023Quote: ... As a negative control for pull-downs assays the eGFP plasmid (Clontech Laboratories, USA) was transfected.
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bioRxiv - Molecular Biology 2021Quote: ... two to three amino acids in GFP-SP-BAP were replaced by alanine for each construct using the CloneAmp HiFi PCR Premix (Takara Bio) according to the manufacturer.
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bioRxiv - Biochemistry 2021Quote: ... Add 25 mL 200 g/L glucose and 25 mL 20 g/L amino acid drop-out mix (Clontech. Inc., Japan) solution to prepare the medium] ...
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bioRxiv - Neuroscience 2022Quote: ... Pak1 fragment (amino acids 270–521) was PCR-amplified from pCMV6M-Pak1 and subcloned into pCold-Pros2 vector (Takara Bio Inc.) to generate pCold-Pros2-Pak1cat ...
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bioRxiv - Neuroscience 2023Quote: ... lysates were centrifuged at 31,000 x g for 45 min at 4°C and the supernatant was mixed with nickel-nitrilotriacetic acid (Ni-NTA) beads (Takara, 635653) at 4°C for 2 h ...
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bioRxiv - Plant Biology 2020Quote: Y1H assay was performed using Matchmaker Gold Yeast One-Hybrid Library Screening System (Clontech, USA). The nucleotide sequences of promoter region of selected key defense genes having potential RAV1 binding motifs were retrieved by using online tool (https://bioinformatics.psb.ugent.be/plaza/) ...
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bioRxiv - Plant Biology 2021Quote: ... Yeast two hybrid assays were performed using the Matchmaker Gold Yeast Two-Hybrid System (Clontech). The standard protocol given in the kit was followed for Y2H assay ...
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bioRxiv - Plant Biology 2021Quote: ... β‐galactosidase assay was performed according to the manufacturer’s protocol (Matchmaker Two‐Hybrid System; Takara Bio ...
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bioRxiv - Microbiology 2020Quote: The yeast two-hybrid assays were performed in strain Saccharomyces cerevisiae Y2HGold (Takara Bio USA). Combination of the two plasmids encoding the desired fusion proteins were transformed into Y2HGold cells using Frozen-EZ Yeast Transformation II Kit (Zymo Research) ...
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bioRxiv - Genetics 2019Quote: The yeast two-hybrid assays were performed using the Matchmaker Yeast Two-Hybrid System (Clontech). Various fragments from OsLUGLWT and OsLUGLkkx were cloned into pGBKT7 ...
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bioRxiv - Plant Biology 2020Quote: Yeast two-hybrid assays were performed by the Matchmarker GAL4-based two-hybrid System (Clontech). Constructs were co-transformed into the yeast strain AH109 by the lithium acetate method (Gietz & Woods ...
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bioRxiv - Microbiology 2019Quote: The dual-luciferase assay using an NF-□B–dependent firefly luciferase (pNF-□B-Luc; Clontech) and a Renilla luciferase driven by the thymidine kinase promoter (pRLTK ...
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bioRxiv - Genetics 2022Quote: ... and then yeast two-hybrid assays were performed in a Gal4-based system from Clontech in a diploid strain by mating as previously described (Rossignol et al ...
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bioRxiv - Plant Biology 2022Quote: Nuclear two-hybrid interaction assays were performed using the MATCHMAKER GAL4 two-hybrid system (Clontech). The CWLP coding region without signal peptide (SP ...
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bioRxiv - Plant Biology 2022Quote: ... The Y2H assays were carried out in the Matchmaker Gold Yeast Two-Hybrid System (Clontech). The pairs of corresponding recombinant constructs fused with pGBKT7 BD and pGADT7 AD were co-transformed into the Y2H Gold yeast competent cells ...
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bioRxiv - Plant Biology 2023Quote: ... Yeast one-hybrid (Y1H) assays were performed according to the manufacturer’s instruction (Clontech, CA, USA). The coding region of PIF4 was amplified and cloned into the yeast GAL4 activation domain (GAL4 AD ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA isolation and real-time PCR assay Total RNA was extacted by RNAiso Plus (TaKaRa) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... cells were pre-treated 1 hour prior to the assay with A/C Heterodimerizer (Takara) diluted at 1:200 in complete media concurrent with JF-Halo or -SNAP dye labeling ...
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bioRxiv - Microbiology 2023Quote: Y2H assays were performed according to Yeastmaker™ Yeast Transformation System 2 User Manual (Clontech). The coding sequences corresponding to HCPro1 ...
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bioRxiv - Cancer Biology 2024Quote: ... and quantitative assays were performed in triplicate using SYBR Premix Ex TaqTM II (Takara #RR820A) in Bio-Rad iQ5 system ...
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bioRxiv - Cell Biology 2024Quote: Following plasmids were used in this assay to generate stable cell lines: pCMV-HRasG12V (Clontech), Piggyback vectors ...
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bioRxiv - Immunology 2022Quote: ... Seq Kit (Takara), as per the manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 kit (Takara) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... mRNA was retro-transcribed by Takara kit (Takara Bio), GAPDH was used as internal reference ...
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bioRxiv - Biophysics 2019Quote: ... D40A mutation and deletion of C-terminal 10 amino acids were introduced into pET15-sfGFP-minD by using the PrimeSTAR Max mutagenesis protocol (TaKaRa, Shiga, Japan). Similarly ...
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bioRxiv - Developmental Biology 2021Quote: ... In-fusion HD kit and In-Fusion Snap Assembly kit (TaKaRa). pRSETa_mEos4b was a gift from Loren Looger (Addgene plasmid #51073 ...
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bioRxiv - Cancer Biology 2023Quote: ... LipofectamineTM 2000 kit and reverse transcription kit were purchased from TaKaRa Company ...
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bioRxiv - Plant Biology 2019Quote: Yeast two-hybrid assays were performed using the Matchmaker™ gold Yeast two-Hybrid Systems (Clontech). Different truncated CDS of EcaICE1 without transcriptional activation activity and EcaSIZ1 were subcloned into pGBKT7 and pGADT7 vectors to fuse with the DNA-binding domain (DBD ...
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bioRxiv - Plant Biology 2020Quote: Yeast two-hybrid assays were performed according to the Yeast Protocol Handbook (Clontech, catalog no. P3024). Briefly ...
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bioRxiv - Cancer Biology 2021Quote: Yeast two-hybrid assay was conducted using Matchmaker GAL4 two-hybrid system 3 (Clontech/Takara, USA). Bait and prey vectors were co-transformed into the yeast strain AH109 (Clontech/Takara ...
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bioRxiv - Cancer Biology 2021Quote: Yeast two-hybrid assay was conducted using Matchmaker GAL4 two-hybrid system 3 (Clontech/Takara, USA). Bait and prey vectors were co-transformed into the yeast strain AH109 (Clontech/Takara ...
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bioRxiv - Molecular Biology 2019Quote: Yeast two-hybrid assays were performed using the Matchmaker GAL4-based two-hybrid system 3 (Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: Y1H assays were performed using the Matchmaker Gold Yeast One-Hybrid Library Screening System (Clontech, USA). The sequence of LhWRKY44 was inserted into the vector pGADT7 as prey ...
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bioRxiv - Developmental Biology 2023Quote: Yeast two-hybrid assays were performed according to the Yeastmaker Yeast Transformation System 2 manual (Clontech). The yeast strain AH109 was co- transformed with an AD-fused and a BD-fused construct using the lithium acetate method ...