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Citations for Takara Bio :
151 - 180 of 180 citations for Corning Hygromycin B Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... and the DNA solution (0.2 ng) was applied to qPCR using SYBR Premis Ex TaqTM (Tli RHaseH Plus) (RR420A; TAKARA). For normalization ...
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bioRxiv - Cell Biology 2021Quote: ... PCR was performed using 1–2 μL of the genomic DNA solution and Tks Gflex DNA polymerase (Takara Bio). The primers are listed in Table S3 ...
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bioRxiv - Microbiology 2022Quote: ... Aer01 tagged with dTomato were labeled by incubating larvae in blocking solution containing Rabbit anti-dsRed (Takara Bio #6324960) at 1:500 dilution ...
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bioRxiv - Neuroscience 2023Quote: ... the solution was replaced with a 0.5% BSA and 0.2% Triton X/PBS solution (PBST) containing a 1:1,000 dilution of Rabbit anti-DsRed (Takara, #632496) or Chicken Polyclonal anti-GFP antibody (Abcam ...
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bioRxiv - Microbiology 2022Quote: ... Then 50 nl of primer pair solution mixed with 1X SmartChip TB Green Gene Expression Master Mix (Takara Bio) were loaded onto the SmartChip ...
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bioRxiv - Microbiology 2023Quote: ... RT-PCR was performed using 500 ng of RNA in 10 µl volume of Solution with PrimeScript RT (TAKARA) and random hexamer primers ...
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bioRxiv - Cancer Biology 2024Quote: ... Viral supernatants were harvested at 48 and 72 hours post transfection and concentrated with lenti-X concentrator solution (Clontech). TRE-MYC HCECs were infected in triplicate at a low MOI (0.2 ...
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bioRxiv - Biophysics 2024Quote: ... the solution of carboxylated beads was diluted into 400 µL of 1× Phosphate-Buffered Saline (PBS) (Takara Bio, T900) containing 100 mg/mL PLL-g-PEG (SuSoS ...
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bioRxiv - Physiology 2021Quote: ... Brain sections were then incubated overnight at room temperature in blocking solution containing primary antiserum (rat anti-mCherry, Life Technologies M11217, 1:1,000; rabbit anti-dsRed, Clontech 632496 ...
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bioRxiv - Molecular Biology 2021Quote: ... was then added to the supernatant to a final concentration of 5 mM and the solution was subjected to immobilized metal affinity chromatography (IMAC) by incubation with TALON cobalt resin (Takara) for 1 hr at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Global macrophage depletion was carried out using the homodimerizer AP20187 at 10 mg/kg in carrier solution by subcutaneous injection (Cat. # 635058, Clontech) with carrier-treated controls (4% EtOH ...
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bioRxiv - Bioengineering 2022Quote: ... The solution was heated at 65°C for 5 minutes using Takara® PCR Thermal Cycler (Takara Corp., Shiga, Japan), and then gradually cooled down to 30°C over a time span of 10 minutes before settling down to 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... Meanwhile, untreated 6-well cell culture plates (Falcon, 351146) were coated with 15 µg/ml retronectin solution (Takara Bio, T100A) overnight at 4 °C ...
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bioRxiv - Plant Biology 2021Quote: ... Membranes were stained with Ponceau S solution and then incubated with anti-GFP antibody (JL-8 Takara Bio Clontech, Lot #A8034133).
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bioRxiv - Plant Biology 2021Quote: ... Membranes were stained with Ponceau S solution and then incubated with anti-GFP antibody (JL-8 Takara Bio Clontech, Lot #A8034133).
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bioRxiv - Molecular Biology 2022Quote: ... A 0.5 µl aliquot of the resulting genomic DNA solution was used as the template for genotyping purposes in 25 µl PCR reactions with Ex Taq DNA polymerase (TaKaRa, RR001). PCR products were subcloned with the TOPO TA cloning Kit (Invitrogen ...
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bioRxiv - Genomics 2022Quote: ... colonies were picked into 96-well plates and a small proportion of cells from each colony were used for DNA extraction using Quick Extract solution (Epicenter) and direct PCR with Prime STAR® GXL DNA Polymerase (Clontech). PCR amplicons were sequenced by Sanger to verify the deletion (SFigure 17).
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bioRxiv - Microbiology 2021Quote: ... Transferred RNA was UV-crosslinked to membrane and hybridized with [γ- 32P] 5’-end labeled RFP-spacer specific probe (RFP_crRNA_probe, Supplementary Table 2) in ExpressHyb solution (Clontech Laboratories, Inc) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... containing 0.5 ml of a 1:1 solution of phosphate-buffered saline (PBS):FBS supplemented with ribonuclease inhibitor (1:100; Takara Bio). For some samples ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA extraction from culture cells was conducted by washing the cells with HEPES buffered saline solution and soaking the cells in RNAiso Plus (Takara Bio). Total RNA was isolated according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... The ICELL8® 3’ DE Chip was placed on the ICELL8® MSND and loaded 50 µL of RT-PCR solution (Takara Bio ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... 40 μL nematode liquid was transferred to a 1.5-mL Eppendorf tube with 40 μL of nematode lysis solution (Zhang et al., 2017) and 5 μL of protease K (Takara, Dalian, China). The mixture was then subjected to centrifugation at 2,000 ×g for 1 min before heating for 45 min at 65 °C in a constant temperature water bath ...
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bioRxiv - Plant Biology 2022Quote: ... Gaillardia × grandiflora ‘Arizona Apricot’ and Antirrhinum majus using Sepasol RNA I Super G (Nacalai Tesque) and purified with a high-salt solution for precipitation (Takara Bio Inc.). A five-micro gram of total RNA was used for the gel blot analysis ...
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bioRxiv - Genetics 2024Quote: Total RNA was extracted from 250 µl of EDTA-anticoagulated venous blood samples collected on-site using RNAiso Blood solution (Cat# 9112; Takara Bio, Japan) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... floating coronal sections were rinsed in PBS and blocked for 1–2 hr at room temperature in a solution of 10% normal goat serum and 0.5% Triton X-100 dissolved in PBS and then incubated in blocking solution containing rabbit anti-DsRed polyclonal antibody (1:1000; Takara Bio, Mountain View, CA) with gentle agitation at 4°C for 18–22 hr ...
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bioRxiv - Cancer Biology 2022Quote: ... pooled and filtered through a 0.45 μm syringe filter and then concentrated using the Lenti-X Concentrator solution (Clontech/Takara, Saint-Germain-en-Laye, France). Intestinal WT organoids were expanded 3 days prior to infection in normal growth medium supplemented with 1 μg/ml R-Spondin ...
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bioRxiv - Immunology 2020Quote: ... Then subsequent primary antibody incubation was done overnight at 4 degrees (all primary antibodies were diluted in 1% blocking milk or for Phos-tag with MBL Max blot solution 1 or Takara Western blot immune booster). Proteins of interest were detected with Anti-Rabbit IgG HRP (Cell signaling technologies ...
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bioRxiv - Immunology 2022Quote: ... and integrinβ7+ MCs from both ears of NT mice (n = 5) and MC903-treated mice (n = 6) were collected into CDS sorting solution (Takara Bio Inc, Shiga, Japan) using 96 well plate ...
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bioRxiv - Neuroscience 2022Quote: ... in PBS] for 90min followed by an incubation with a rabbit anti-DsRed antibody (1:2000, in blocking solution; Takara Bio-Living Colors, RRID #632496) overnight at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... pooled and filtered through a 0.45 μm syringe filter and then concentrated using the Lenti-X Concentrator solution (Clontech/Takara, Saint-Germain-en-Laye, France). Intestinal WT organoids were expanded 3 days prior to infection in normal growth medium supplemented with 1 μg/ml R-Spondin ...