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Citations for Takara Bio :
1801 - 1850 of 2119 citations for 7 Benzyloxy 10 11 dihydrodibenzo b f 1 4 thiazepin 11 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... The RNA (1 μg) was applied to generate cDNA by means of PrimeScript™RT Master Mix (Takara, RR036A). The real-time quantitative PCR was performed by using SYBR Green Master Mix (Takara ...
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bioRxiv - Cell Biology 2019Quote: ... For lentiviral transduction WT and Quad-K Jam-C-GFPout were amplified using appropriate primers (Supp. Table 1) and subcloned into pSFFV-WPRE using an infusion reaction according to the manufacturer’s instructions (Clontech).
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bioRxiv - Genomics 2019Quote: ... We spun down the aliquots and re-suspended the pellets in 1 ml DroNc-seq resuspension buffer (1X PBS, 0.01% BSA, 0.16 U/μl RNase Inhibitor (Clontech/TaKaRa, #2313A)) for Tube A and 1 ml 10X Nuclei resuspension buffer (1X PBS ...
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bioRxiv - Microbiology 2021Quote: ... Reverse transcription was carried out with 1 μg RNA using TB Green Premix Ex Taq™ II (Takara, Japan). Targets of around 110 bp were amplified with primers qtaxB-F/R ...
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bioRxiv - Cell Biology 2021Quote: ... PCR was performed using 1–2 μL of the genomic DNA solution and Tks Gflex DNA polymerase (Takara Bio). The primers are listed in Table S3 ...
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Panacea: a hyperpromiscuous antitoxin protein domain for the neutralisation of diverse toxin domainsbioRxiv - Microbiology 2021Quote: ... Filtered lysate was incubated with 1 mL of previously buffer equilibrated Ni-beads (His60 Ni Superflow Resin, TaKaRa, Japan) for 30 minutes ...
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bioRxiv - Microbiology 2021Quote: The LTR promoter of the HIV-1 laboratory strain pNL4-3 was cloned into the pTA-Luc backbone (Clontech) and is henceforth referred to as pTA-Luc-NL4-3 ...
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bioRxiv - Cell Biology 2021Quote: ... Total RNA (1 μg) was converted to cDNA using the PrimeScript™ RT reagent Kit with gDNA Eraser (TAKARA). Quantitative PCR (qPCR ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were washed twice with 20 mL of D-PBS (Nacalai Tesque) and resuspended in CELLBANKER 1 (Takara Bio) at 1 × 107 cells/mL ...
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bioRxiv - Microbiology 2022Quote: The recombinant pGBKT7-TaPHB-1 was transformed into Y2HGold yeast strain following the Yeastmaker Yeast Transformation System 2 (TaKaRa). The transformants were screened on SD/-Trp agar plate ...
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bioRxiv - Microbiology 2022Quote: ... The cDNA was prepared from 1 μg of total RNA using PrimeScript 1st strand cDNA Synthesis Kit (TaKaRa, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... The cDNA was amplified with gene-specific primers (Supplemental Table 1) and SYBR Premix Ex Taq II kit (TaKaRa). Data were analyzed using a 2−ΔΔCt method.
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bioRxiv - Genetics 2021Quote: ... cDNA synthesis was done using 0.1-1 μg approximately of RNA following the manufacturer protocol (Takara Bio, Kusatsu, Japan). We used the 2xPrimeTime® Gene Expression Master Mix (Takara Bio) ...
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bioRxiv - Cancer Biology 2021Quote: ... with PCR using the primers listed in Table 1 and cloned into pmCherry-C1 (CMV promoter; Takara Bio Inc.). pEGFP-C3 K19 WT ...
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bioRxiv - Genetics 2022Quote: ... 1 µg of total RNA was used for reverse transcription using PrimeScript RT Reagent Kit (Cat#RR037A, TaKaRa, Japan). Quantitative polymerase chain reaction (qPCR ...
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bioRxiv - Cell Biology 2019Quote: ... Infected cells were cultured on Geltrex in NPC medium with 1 × RevitaCell supplement and 2 ug/ml Doxycycline (Clontech) to induce NGN2 gene expression ...
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bioRxiv - Cell Biology 2019Quote: ... 10 µg of the lysates were separated by SDS-PAGE and blots were probed with a 1:2,000 dilution of mouse anti-GFP antibody (Clontech), followed by 1:5000 anti-mouse Starbright Blue 700 (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were analyzed on bleach agarose gels (0.06% bleach, 1% (w/v) agarose) for visualization of the RNA and on WB (anti-His antibody, Clontech) for visualization of His6-tagged Nsp1.
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bioRxiv - Pathology 2020Quote: ... A total of 1 × 106 cells were used for total RNA extraction using RNAiso Plus reagent (Cat.#9109, TaKaRa), followed by treatment with 10 U of recombinant DNase I (Cat.#2270A ...
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bioRxiv - Molecular Biology 2021Quote: ... The insoluble material was removed by centrifugation at 150,000 ×g and the supernatant was added to 1 ml pure TALON resin (Clontech) and 20 mM imidazole and rock slowly overnight at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... 35 mm wells of MIN6 cells were co-transfected with 2.5 µg of pX330 containing gRNAs and 1 µg of pEGFP-C2 (Clontech). After 48 h ...
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bioRxiv - Microbiology 2020Quote: ... primary antibody was prepared in PBS containing 1% non-fat milk using anti-hexahistidine antibody (Takara Bio, catalog #631212) at a dilution of 1:3000 ...
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bioRxiv - Microbiology 2020Quote: ... 1) were annealed to a complementary 20-nt primer containing a 5’-fluorescein label (5’FAM-GUCAUUCUCCUAAGAAGCUA-3’, Takara). To perform the primer extension assay ...
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bioRxiv - Genomics 2021Quote: ... or SL medium (for E14-STNΔTsixP) and transduced the next day with 1ml of 5:1 concentrated (lenti-X, Clontech) and filtered viral supernatant with 8 ng/µl polybrene (Sigma Aldrich) ...
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Mck1 defines a key S-phase checkpoint effector in response to various degrees of replication threatsbioRxiv - Molecular Biology 2019Quote: ... DNA was transferred to HyBond N+ in transfer buffer (0.4 M NaOH, 1 M NaCl) and UV cross-linked before hybridization with a random primed probe (Takara) overnight at 42°C and washed twice for 20 min with 0.5× SSC 0.1% SDS at 65°C.
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bioRxiv - Cell Biology 2021Quote: ... 1-2ug of RNA was used to synthesise cDNA using Superscript III or PrimeScript kit (Thermo Fisher Scientific, Takara). Quantitative PCR (qPCR ...
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bioRxiv - Genomics 2021Quote: ... Non-tissue culture treated 12-well plates were prepared by incubating 1 mL PBS + 25 μg/mL Retronectin (Takara) overnight at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... Primary antibodies for embryo staining were used at the following final dilutions: rabbit anti-DsRed (1:400, Clontech 632496), rat anti-tropomyosin (1:200 ...
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bioRxiv - Developmental Biology 2021Quote: ... The cDNA was synthesized with 1 ug total RNA using the PrimeScriptTM RT Reagent Kit with gDNA Eraser (TaKaRa). qRT-PCR was performed using a qTOWER3G system (analytikjena ...
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bioRxiv - Plant Biology 2021Quote: ... The samples were immobilized by molten 1.5% low-melting point agarose (Agarose LMT 1-20K, PrimeGel, Takara Bio Inc.) spread thinly on a glass-bottom dish and immediately covered in water ...
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bioRxiv - Immunology 2020Quote: ... 1 μg of RNA per sample was used for first-strand synthesis by SMARTer PCR cDNA Synthesis Kit (Clontech). For quantitative RT-PCR (qPCR) ...
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bioRxiv - Immunology 2021Quote: ... 100,000 cells were sorted into 200 uL PBS with 1 uM DTT and 5 uL RNase Inhibitor Cocktail (Takara); for ex vivo culture experiments ...
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bioRxiv - Genetics 2020Quote: ... Total RNA (1 mg) was reverse transcribed using the PrimeScript RT Reagent Kit with the gDNA Eraser (TAKARA, Japan). Quantitative PCR reactions were performed using the gene-specific primers of FLC and FT (Table S1 ...
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bioRxiv - Microbiology 2022Quote: ... A 5 µl aliquot was removed from each sample for immunoblots using mouse anti-GFP (1:5,000, Clontech #632381) to detect A3-EGFP ...
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bioRxiv - Biochemistry 2022Quote: ... MBL) and HRP-labeled IgG detector (1/5,000 dilution from the product, Western BLoT Rapid Detect v2.0, Takara Bio) were used as primary and secondary antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... Complementary DNA (cDNA) was prepared from 1 μg of RNA using PrimeScriptTM RT Master Mix (catalog no. RR036A, Takara). qPCR was performed using TB Green Premix Ex TaqTM (catalog no ...
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bioRxiv - Molecular Biology 2023Quote: ... and 1 ug of RNA was reverse-transcribed into cDNAs using the Prime Script RT Reagent Kit (Takara, RR037B) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 μg of total RNA was reverse transcribed using the double primed RNA to cDNA EcoDry premix (Takara Bio). Primers were designed targeting FLuc ...
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bioRxiv - Cell Biology 2023Quote: ... 2xmCh-KIF5C in pcDNA3.1 was cloned by inserting in-frame KIF5C full length from EGFP-KIF5C (a gift from Anthony Brown) 2xmCh sequence from KIF5C(1-560)-2xmCh-EF(C) into pcDNA3.1 using InFusion Cloning kit (Takara). EGFP-Rab7A (Addgene #28047 ...
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bioRxiv - Genomics 2023Quote: ... The ligation reaction (1 µL) was transformed into 50 µL of Escherichia coli HST08 premium electro-cells (Takara, Japan) using a Gene Pulser Xcell (Bio-Rad ...
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bioRxiv - Plant Biology 2023Quote: ... The full-length and truncate CDS of TaNAM-A1a (residues 1–220) were cloned into bait vector pGBKT7 (Clontech) respectively to generate bait plasmids BD-NAM-A1full and BD-NAM-A11-220 ...
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bioRxiv - Molecular Biology 2023Quote: ... Equal loading of the GFP tagged E2F1 proteins was determined by the GFP antibody (Clontech, lot. 1404005; 1:2000) the secondary antibody anti-rabbit HRP (Na934v ...
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bioRxiv - Biochemistry 2023Quote: ... cDNA was prepared from 1 µg of total RNA using the PrimeScript RT Reagent Kit with gDNA Eraser (Takara). Real-time polymerase chain reaction (real-time PCR ...
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bioRxiv - Neuroscience 2023Quote: ... the solution was replaced with a 0.5% BSA and 0.2% Triton X/PBS solution (PBST) containing a 1:1,000 dilution of Rabbit anti-DsRed (Takara, #632496) or Chicken Polyclonal anti-GFP antibody (Abcam ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was synthesized from 1 µg of each RNA sample using PrimeScript RT Reagent Kit with gDNA Eraser (Takara), and the primers used for qRT-PCR were designed using Primer3web ...
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bioRxiv - Neuroscience 2024Quote: ... HEK293 cells were first incubated with ARIAD ligand at a concentration of 1 µM (AL; D/D Solubilizer; Takara) for the indicated times ...
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bioRxiv - Plant Biology 2020Quote: ... An additional BbsI site in the pDONR backbone was eliminated by site-directed mutagenesis using primers noBbsI_F and noBbsI_R (Supplemental Table 1) followed by an In-Fusion reaction (Takara Bio USA). In order to yield the final binary vectors ...
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bioRxiv - Microbiology 2020Quote: ... CTCF BS1 and CTCF BS2 were mutated by site-directed PCR mutagenesis using the primers detailed in Table 1 and Prime Star Max (Takara) mutagenesis kit following the manufacturer’s protocols and confirmed by sequencing ...
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bioRxiv - Developmental Biology 2021Quote: Cells were lysed and RNA was reverse transcribed and amplified according to the SMARTer Ultra Low RNA Kit for Illumina Sequencing (version 1, Clontech). Libraries were prepared from cDNA using the NEBNext Ultra DNA library preparation for Illumina sequencing with indexed adaptors (New England Biolabs) ...
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bioRxiv - Developmental Biology 2021Quote: Two-hybrid screening and assays were performed as described in the Matchmaker™ Pretransformed Libraries User Manual (Takara PT3183-1) using a Drosophila Mate & Plate™ Library - Universal Drosophila (Normalized A+ RNA isolated from embryo (~20 hr) ...