Labshake search
Citations for Takara Bio :
1651 - 1700 of 1701 citations for Hepatitis A Virus Cellular Receptor 1 HAVCR1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2019Quote: 3000 quiescent satellite cells were lysed after FACS by sorting directly into a 0.2ml tube containing 1 μl SMART-Seq Reaction Buffer (95% SMART-Seq 10x lysis buffer containing 5% SMART-Seq RNAse Inhibitor, Takara Bioscience, Cat. 634890) in 8μl ddH20 ...
-
bioRxiv - Cancer Biology 2019Quote: ... First-strand cDNA was synthesized from 1 μg of total RNA using the PrimeScript™ RT reagent Kit with gDNA Eraser (TaKaRa, Dalian, China). SYBR Premix Ex Taq II (TaKaRa ...
-
bioRxiv - Plant Biology 2019Quote: ... 1.5 kb) was amplified with P1 and P2 primers (see Supplementary Table 1) from Col genomic DNA using PrimeStarMax (Takara Bio, Kusatsu, Japan) and inserted in HindIII-XbaI digested pGWB51131 by the SLiCE method32 to give p511G1pro ...
-
bioRxiv - Genetics 2020Quote: ... RNA was extracted from samples using TRIzol and cDNA synthesized from 1 μg RNA template using PrimeScript™ RT reagent Kit with gDNA Eraser (TAKARA BIO INC). The sequences of primers used for amplification are shown in Table S6 ...
-
bioRxiv - Biochemistry 2021Quote: ... After sonication and centrifugation (20 000 g, 1 h, 4 °C) the supernatant was mixed with Talon Metal Affinity Resin (Takara Bio USA, Inc.). After 1 hour incubation at 4 °C ...
-
bioRxiv - Cancer Biology 2020Quote: ... the pEF1α-tdT/FLuc2 vector was modified to replace tdT with the fluorescence reporter zsGreen 1 (zsG) obtained from the pLVX-ZsGreen1-N1 Vector (Cat. 632565, Takara Bio USA, Inc.) and FLuc2 was replaced with the Antares2 sequence obtained from pcDNA3-Antares2 c-myc ...
-
bioRxiv - Cancer Biology 2021Quote: Total RNA (1 μg) was reverse transcribed to cDNA using the PrimeScriptTM RT reagent kit with gDNA Eraser (Cat#: RR047A, TaKaRa Bio, Shiga, Japan). qRT–PCR was performed using a LightCycler 480 II instrument (Roche Molecular Systems ...
-
bioRxiv - Molecular Biology 2022Quote: ... Finally RNA was isolated from the eluates by phenol-chloroform-Isoamyl alcohol (25:24:1) pH 4.5 extraction and cDNA was prepared using 1st strand cDNA synthesis kit (Takara Bio Inc., Shiga, Japan) as described in above RNA analysis section ...
-
bioRxiv - Plant Biology 2022Quote: ... Total RNA was isolated using Quick-RNA miniprep kit (ZymoResearch, CA, USA) and 1 μg of RNA was used to generate cDNA using SMART MMLV reverse transcriptase (Takara Bio USA, Inc.). Transcript abundance of an ABA biosynthesis gene ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: The cDNA was synthesized using 1 µg of RNA from each treatment using the PrimeScript™ RT reagent Kit with gDNA Eraser (TaKaRa Dalian, China). RT-qPCR was performed on a Bio-Rad CFX96 (Bio-Rad ...
-
bioRxiv - Microbiology 2023Quote: ... Approximately 1 µg of total RNA was used for reverse transcription using the PrimeScript™ RT reagent kit with gDNA Eraser (Takara, Beijing, China). After the transcribed cDNAs were 5-fold diluted ...
-
bioRxiv - Biochemistry 2024Quote: ... Sub-library specific primers were then used to amplify 1 ul of cDNA template for 24 cycles using Advantage 2 PCR Mix (Takara Bio, Cat. 639206). Finally ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... we then added 1 mL of Super Optimal Broth with Catabolite Repression Medium (SOC, provided with the electrocompetent cells (Takara, Japan, product #9027), shaking the cells at 230 RPM at 37°C for 1.5 hours (Infors HT ...
-
bioRxiv - Neuroscience 2023Quote: ... were transiently cotransfected with a 2:1 mixture of Nav1.7-expressing plasmid8 and a pIRES2-ZsGreen bicistronic plasmid (Takara Bio, San Jose, CA) expressing ZsGreen and mouse FHF2B proteins.10 The same pIRES2-ZsGreen plasmid without FHF2 coding sequence served as the control ...
-
bioRxiv - Molecular Biology 2023Quote: ... the first-strand cDNA was synthesized from 1 μg total RNA using PrimeScript™ II 1st Strand cDNA Synthesis Kit (Takara, Beijing, China) following the manufacturer’s instructions.
-
bioRxiv - Cell Biology 2023Quote: ... containing a single-vector Tet-on component and were cultured in the presence of 1 µg/ml doxycycline (Clontech, Mountain View, CA, USA) during induction.
-
bioRxiv - Plant Biology 2023Quote: Genomic DNA was removed from 1 μg purified RNA using gDNA Eraser (PrimeScript RT Reagent Kit, Perfect Real Time) following manufacturer’s instructions (Takara Bio Inc., Kusatsu, Japan). Reverse transcription and cDNA was synthesized using PrimeScript Reverse Transcriptase (TaKaRa Bio) ...
-
bioRxiv - Immunology 2024Quote: ... 0.5mL T cells at 2 × 106 cells mL-1 were mixed with 1.5mL lentiviral supernatant and centrifuged in 24-well plates pre-coated with 7 µg mL-1 Retronectin (Takara Bio, San Jose, USA) at 1000 x g for 20min ...
-
bioRxiv - Cell Biology 2024Quote: A total of 250 pg to 10 ng RNA per sample was used as input material for sequencing libraries (1–500 ng for small RNA libraries) using the SMARTer Stranded Total RNA-Seq Kit V2 (Takara Bio USA, Inc.) following the manufacturer’s recommendations ...
-
bioRxiv - Plant Biology 2024Quote: ... First-strand cDNA was reverse transcribed from 1 μg of total RNA using the PrimeScriptTM RT reagent kit (AK4201; Takara Bio, Kusatsu, Japan) according to the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2019Quote: ... A first-strand cDNA was obtained from 1 μg of total RNA using a PrimeScript® RT reagent kit (Takara Bio, Inc., Otsu, Japan). The sequences of candidate genes were obtained from the local alkaligrass EST database using a BLASTn program ...
-
bioRxiv - Molecular Biology 2020Quote: ... The first-strand cDNA was synthesized with 1 μg of total RNA using a PrimeScript™ 1st Strand cDNA Synthesis Kit with gDNA Eraser (TaKaRa Biotechnology, Dalian, China). For sequencing analysis of the PxRdls ...
-
bioRxiv - Developmental Biology 2020Quote: ... Lentiviral supernatant titers were determined by Lenti-X p24 Rapid Titer Kit (supplemental Table 1) according to manufacturer’s protocol (Takara Bio USA, Inc. California, U.S.A).
-
bioRxiv - Molecular Biology 2019Quote: HEK293 cells were transfected with p122RhoGAP/DLC-1 siRNA (sense, 5′-GAAACGCCUUAAGACACUATT-3′; antisense, 5′-UAGUGUCUUAAGGC GUUUCTT-3′; TaKaRa Biotechnology Co., Ltd., Kyoto, Japan), IQGAP1 siRNA (s16837 ...
-
bioRxiv - Microbiology 2021Quote: ... total RNA (1 μg) was used to synthesize first-strand cDNA by employing an M-MLV Reverse Transcriptase (code no. 2641A, TaKaRa Bio Inc., Dalian, China) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... The knockout construct was developed through In-Fusion using the purified amplicons based on the manufacturer’s instructions with the insert to vector ratio of 2:1 (Takara Bio, Mountain View, CA, USA) and transformed into E ...
-
bioRxiv - Cell Biology 2019Quote: ... The cDNA was synthesized by reverse transcription using 1 μg of total RNA as a template with a PrimeScript™ RT Reagent Kit with gDNA Eraser (Takara, Beijing, China, RR047A). The SYBR® Premix Ex Taq TM II kit (Takara ...
-
bioRxiv - Microbiology 2021Quote: ... The PCR fragment was digested with BamHI and SalI and ligated into BamHI and SalI double digested pCOLD-1 vector (Takara Bio Inc, Shiga, Japan) to produce pCOLD1-EhPTEN1 plasmid ...
-
bioRxiv - Neuroscience 2020Quote: ... and a portion (1 ng) of the RNA were subjected to reverse transcription (RT) with a SMARTer™ PCR cDNA Synthesis Kit (Clontech, Mountain View, CA). Then ...
-
bioRxiv - Immunology 2020Quote: cDNAs encoding EqPD-1 and EqPD-L1 were amplified by PCR using gene-specific primers with restriction enzyme cleavage sites (Supplementary Table 1) and subcloned into the multicloning site of pEGFP-N2 (Clontech, Palo Alto, CA, USA).
-
bioRxiv - Evolutionary Biology 2022Quote: ... with 0.1 µL of Taq Polymerase 5 U.µL-1 (TaKaRa Ex Taq® Kit MgCl2 Free Buffer) (TaKaRa Ex Taq, TaKaRa Bio, Shiga, Japan), 2.5 µL PCR Buffer 10X ...
-
bioRxiv - Cancer Biology 2022Quote: Human c-Jun sequence was amplificated using the cDNA of MCF7-BM02-1 and then transferred to pMXd3-PEF1-IRES-Puro vector using an In-Fusion Advantage PCR Cloning Kit (Clontech Laboratories Inc., CA, USA). The sequence encoding the transactivation domain of c-Jun in pMXs-Jun-IH was kindly provided by Dr ...
-
bioRxiv - Molecular Biology 2024Quote: ... From cortical neurons subjected to OGD we generated 1) library to detect circularRNAs and mRNA and lncRNAs using SMARTer® Stranded Total RNA Sample Prep Kit (Takara Bio USA, Inc.) and 2 ...
-
bioRxiv - Cell Biology 2024Quote: A plasmid expressing Ub-X-YFP-FLAG was constructed as follows: (1) the pHA-Ub-YFP: YFP moiety of pEYFP-N1 (Clontech, Mountain View, CA, USA) was amplified using PCR with primers HA-Ub-R-YFP f and r ...
-
bioRxiv - Neuroscience 2023Quote: ... A total of 1 µg of RNA was reverse transcribed to complementary DNA using the PrimeScript™ RT reagent Kit (Takara Bio, Otsu, Japan), according to the manufacturer’s protocol ...
-
bioRxiv - Bioengineering 2023Quote: ... Protease inhibitors and 10% glycerol were added to the supernatant before loading it onto 1 mL of Talon metal affinity resin (Clontech, Mountain View, CA, #635502). After a 20 mL buffer wash ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: The Northern dot blots containing cDNAs from primary human tumors (T) with paired adjacent normal tissues (N) and cancer cell lines were obtained from Clontech (Cancer Profiling Array, #7840-1). The blots contained normalized cDNA isolated from tumors and the corresponding adjacent normal tissues from individual cancer patients ...
-
bioRxiv - Zoology 2021Quote: ... The reverse transcription of 1 μg of total RNA was performed using PrimeScript® RT reagent kit with gDNA Eraser (RR047A, Takara Bio Inc., Dalian, China). The gene expression was determined through RT-PCR ...
-
bioRxiv - Microbiology 2022Quote: ... and A549-MxB(1-715)-MxB(26-715) cells expressing untagged MxB(1-715) and MxB(26-715) using the pLKOD-Ires-Puro vector (Clontech Takara Bio, Mountain View, United States). MeWo cells (kind gift from Graham Ogg ...
-
bioRxiv - Neuroscience 2020Quote: ... with a total of 1 µg of good quality total RNA as input and enriched using the SMARTer Apollo NGS library prep system (Takara Bio USA, Mountain View, CA). Sequencing libraries were prepared using the NEBNext Ultr II Directional RNA Library Prep Kit for Illumina (New England Biolabs) ...
-
bioRxiv - Neuroscience 2021Quote: ... The cDNAs that were used for RACE were synthesised from 1 µg of polyA+ RNA using a SMART RACE cDNA Amplification Kit (Clontech Laboratories, Inc., Palo Alto, CA) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: ... cDNA was synthesized from 1 ng RNA using the SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing (Takara Bio Europe SAS). Libraries were prepared from the purified cDNA using the Nextera™ XT DNA Library Preparation Kit (Illumina) ...
-
bioRxiv - Biochemistry 2023Quote: ... HEK293T or UFSP2 KO HEK293T cells (16) were transfected with the aforementioned pX330 vectors together with pEGFP-C1 (#6084-1, Clontech Laboratories, Mountain View, CA, USA) and cultured for 2 d ...
-
bioRxiv - Plant Biology 2023Quote: ... Yeast-two-hybrid library screens were carried out according to Matchmaker® Gold Yeast Two-Hybrid System User Manual (Takara Clontech Bio Europe, PT4084-1) using a cDNA library of M ...
-
bioRxiv - Plant Biology 2023Quote: ... Yeast-two-hybrid library screens were carried out according to Matchmaker® Gold Yeast Two-Hybrid System User Manual (Takara Clontech Bio Europe, PT4084-1) using a cDNA library of M ...
-
bioRxiv - Physiology 2023Quote: ... 1 μg of total RNA was reverse transcribed using RNA to cDNA EcoDry™ Premix (Double Primed) (Takara Bio USA Inc., San Jose, CA, USA) according to the manufacturer instruction ...
-
bioRxiv - Physiology 2024Quote: Total RNA (1 µg) was used as input for SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian (Cat. # 634875; Takara, Saint-Germain-en-Laye, France). Sequencing was performed on the NextSeq 2000 instrument (Illumina ...
-
bioRxiv - Physiology 2024Quote: Libraries were prepared by the Van Andel Genomics Core (Grand Rapids, MI) from 1 ng of total RNA using Takara SMART-Seq Stranded Kit (Takara Bio USA, Mountain View, CA) per the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2019Quote: ... and 19 and BIRC2 was amplified using specific primers (Supplementary File 1) and cloned downstream of luciferase gene into pcDNA6/Luc(30) using an In-Fusion HD cloning kit (Takara Bio USA, Inc., Mountain View, CA). The recombinant pcDNA6/LUC-DUSP (or pcDNA6/LUC-DUSP-211Δ) ...
-
bioRxiv - Microbiology 2021Quote: ... We PCR amplified each region from 1 μL of the converted gDNA using TaKaRa EpiTaq HS for bisulfite-treated DNA (Takara Bio USA; Mountain View, CA, USA) per manufacturer’s instructions with primers designed using MethPrimer ...