7 BROMO 2 3 4 5 TETRAHYDRO 1H BENZO E 1 4 DIAZEPINE 2 CARBOXYLIC ACID METHYL ESTER citations for Takara Bio

Quote: ... and incubated in primary antibody (1:1,000 mouse anti-calbindin, Sigma; 1:500 rabbit anti-DsRed, Clontech; 1:1,000 guinea pig anti-vGluT1 ...

Identification of the main barriers to Ku accumulation in chromatin

Madeleine Bossaert, et al., bioRxiv - Molecular Biology 2024

Quote: ... 4.9 μg DNA was transfected per dish containing a 1:1 molar ratio of pmCherry-N1 (Clontech) and of the pUC18-GFP or linear GFP substrate ...

Compartmentalization and synergy of osteoblasts drive bone formation in the regenerating fin

N Cudak, et al., bioRxiv - Developmental Biology 2023

Quote: ... primary antibodies (chicken anti-GFP, Abcam, ab13970, 1:2000 and rabbit anti-DsRed, Clontech, 632496, 1:200) were incubated overnight at 4°C ...

Evolution of resistance to Irinotecan in cancer cells involves generation of topoisomerase-guided mutations in non-coding genome that reduce the chances of DNA breaks

Santosh Kumar, et al., bioRxiv - Cancer Biology 2023

Quote: ... 1-st PCR (PCR 1) was performed using Titanium Taq DNA Polymerase (# 639209, Takara Bio, CA, USA). Separation of the PCR products from primers and gel purification was done by QIAquick PCR & Gel Cleanup Kit (Qiagen ...

Flap endonuclease 1 repairs DNA-protein crosslinks via ADP-ribosylation

Yilun Sun, et al., bioRxiv - Molecular Biology 2023

Quote: ... pLKO.1-shFEN1 or pLKO.1 control vector was simultaneously transfected into LentiX293T (Cat# 632180 Clontech, Japan) with packaging plasmid ...

An in vivo reporter for tracking lipid droplet dynamics in transparent zebrafish

Dianne Lumaquin, et al., bioRxiv - Cell Biology 2020

Quote: ... the PLIN2 cDNA was inserted on the 5’ end of pME-tdTOMATO using In-Fusion HD Cloning Plus (Takara, Mountain View, USA, Catalog #638920). Gateway cloning using the Gateway LR Clonase Enzyme mix (Thermo Fisher ...

Circulating mast cell progenitors migrate to atopic dermatitis-like skin then adapt locally

Yuki Honda Keith, et al., bioRxiv - Immunology 2022

Quote: ... and integrinβ7+ MCs from both ears of NT mice (n = 5) and MC903-treated mice (n = 6) were collected into CDS sorting solution (Takara Bio Inc, Shiga, Japan) using 96 well plate ...

The FW2.2/CNR protein regulates cell-to-cell communication in tomato by modulating callose deposition at plasmodesmata

Arthur Beauchet, et al., bioRxiv - Plant Biology 2023

Quote: ... The FW2.2 full-length coding sequence was amplified from cDNAs prepared from tomato (cv. AC) fruits at 5 DPA using PrimeSTAR MAX DNA polymerase (TAKARA BIO Inc., Kusatsu, Japan) and primers including the attB sites (Supplemental Table 1) ...

Missense mutations in Myc Box I influence MYC cellular localization, mRNA partitioning and turnover to promote leukemogenesis

Nancy BJ Arthur, et al., bioRxiv - Cancer Biology 2023

Quote: Retroviral supernatant was collected two and three days after transfection of GP2-293T cells and concentrated onto wells of a 6 well plates coated with Retronectin (Takara Bio, 5 ug/ml) by spinning at 2500g for 90 minutes at 32° C ...

The UBR box E3 ligases Poe and Hyd are required for efficient Pericentrin degradation

Ramya Varadarajan, et al., bioRxiv - Developmental Biology 2022

Quote: ... Primary antibodies were anti-PLP (N-terminus; 1:5000, Rogers, 2008) anti-GFP (JL8; 1:2000 – 5000; Clontech); anti-alpha-Tubulin (DM1A ...

Social deficits induced by pervasive environmental stressors are prevented by microbial or dopaminergic modulation

Caroline J. Smith, et al., bioRxiv - Neuroscience 2022

Quote: ... sections were incubated with primary antibody (anti-Th [mouse] 1:1500, Immunostar; anti-dsRed [rabbit] 1:1500, Clontech) overnight at RT shaking ...

Zebrafish Polycomb repressive complex-2 critical roles are largely Ezh2- over Ezh1-driven and concentrate during early embryogenesis

Gabriel A. Yette, Scott Stewart, Kryn Stankunas, bioRxiv - Developmental Biology 2021

Quote: ... Samples were incubated with chicken anti-GFP 1:1000 (Aves) and rabbit anti-DsRed 1:500 (Clontech/Takara) antibodies diluted in blocking solution overnight at 4°C ...

Zebrafish Polycomb repressive complex-2 critical roles are largely Ezh2- over Ezh1-driven and concentrate during early embryogenesis

Gabriel A. Yette, Scott Stewart, Kryn Stankunas, bioRxiv - Developmental Biology 2021

Quote: ... Samples were incubated with chicken anti-GFP 1:1000 (Aves) and rabbit anti-DsRed 1:500 (Clontech/Takara) antibodies diluted in blocking solution overnight at 4°C ...

Telomeric DNA breaks in human induced pluripotent stem cells trigger ATR-mediated arrest and telomerase-independent telomere length maintenance

Katrina N Estep, et al., bioRxiv - Cell Biology 2023

Quote: ... Cells were then induced for the indicated amount of time with 1 μM Shield-1 ligand (Takara bio) to stabilize the TRF1-FokI protein and 1 μM 4-OHT (Cayman Chemical ...

Arabidopsis ABCC4 encodes a cytokinin efflux transporter and is involved in root system development

Takuya Uragami, et al., bioRxiv - Plant Biology 2024

Quote: ... Transformed yeast cells were grown under selective conditions in a minimal medium (46.7 g L-1 Minimal SD Agar Base [TaKaRa], 0.78 g L-1 uracil dropout supplement [TaKaRa]). Cytokinin transport assays were performed according to the method described by Zhao et al ...

Defining totipotency using criteria of increasing stringency

Eszter Posfai, et al., bioRxiv - Cell Biology 2020

Quote: ... mouse anti-mCherry 1:500 (632543, Clontech); chicken anti-Gfp 1:500 (ab13970 ...

R7 photoreceptor axon targeting depends on the relative levels of lost and found expression in R7 and its synaptic partners

Jessica Douthit, et al., bioRxiv - Developmental Biology 2021

Quote: ... rabbit anti- dsRed (1:500; Takara Bio), guinea pig anti-Loaf (1:400 ...

Dynamic adult tracheal plasticity drives stem cell adaptation to changes in intestinal homeostasis

Jessica Perochon, et al., bioRxiv - Developmental Biology 2021

Quote: ... rabbit anti-DsRed 1:1000 (Clontech, #632496), mouse anti-Arm 1:3 (Hybridoma Bank ...

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